IndraLab

Statements

UCHL5 is ubiquitinated. 20 / 20
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"In this study we find that, while incorporated in the 26S proteasome complex, Adrm1, S5a, Rpt5, and Uch37 are ubiquitinated in situ by proteasome-associating ubiquitination enzymes."
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"S5a, Adrm1, Uch37, and Rpt5 are ubiquitinated on the 26S proteasome."
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"Ubiquitinated UCHL5 was found to be enriched in UCHL5 C88A samples by mass spectrometry analysis ( xref , xref )."
31703099
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"Only the four members of the UbE2D family were able to strongly promote ubiquitination of Adrm1, S5a, Rpt5, and Uch37 on the 26S proteasome ( xref )."
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"In the control assays, overexpression of Myc-Ub promoted ubiquitination of Adrm1, S5a, Rpt5, and Uch37 but not Rpn2 (compare lanes 1 and 2 in xref )."
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"Thus members of the UbE2D family of E2s are capable of promoting ubiquitination of S5a, Adrm1, Uch37, and Rpt5 on the 26S proteasome both in vitro and in cells."
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"Ubiquitination of S5a and Uch37 on PA700 was strongly promoted by addition of UbE3A, whereas Rpt5 ubiquitination was only mildly increased ( xref , lane 6)."
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"The robust accumulation of ubiquitin conjugates, ubiquitinated β-catenin, and ubiquitinated UCHL5 were clearly observed in this set of experiments as seen previously (Figs xref – xref )."
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"We next examined the effect of these polyUb chains on S5a, Adrm1, Uch37, and Rpt5 ubiquitination by supplementing increasing concentrations of these chains into in vitro ubiquitination assays."
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"In situ ubiquitination of Adrm1, S5a, Rpt5, and Uch37 is accomplished by proteasome-associating UbE1 and E3 Ub ligases together with the UbE2D family of E2s."
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"Our study also shows that binding of polyUb chains on the 26S proteasome inhibits ubiquitination of Adrm1, S5a, Rpt5, and Uch37 in vitro."
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"In support of this, we found that Adrm1 preferred to bind ubiquitinated Uch37 compared with nonubiquitinated Uch37 (Tian and Liu, unpublished result)."
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"Presumably, ubiquitinated Uch37 offers an additional binding event, in which the Ub moieties conjugated on Uch37 directly interact with the N-terminal Pru domain of Adrm1, as the Pru domain is capable of binding monoUb and polyUb chains ( xref ; xref )."
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"Furthermore UCHL5 C88A expression led to a high molecular weight laddering of UCHL5 itself as detected by IB with a UCHL5 antibody, presumably representing ubiquitinated UCHL5 ( xref ) [ xref – xref ]."
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"While ubiquitinated UCHL5 was identified to be more abundant by mass spectrometry in UCHL5 C88A but not USP14 C114A samples ( xref )."
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"While the IP-MS data suggest USP14 is required for deubiquitination of multiple proteasomal subunits ( xref ), USP14 C114A did not affect UCHL5 ubiquitination as shown by the lack of the UCHL5 laddering ( xref )."
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"The ubiquitin-conjugating enzyme, UBE2W catalyzes ubiquitination on the N-terminal end of UCHL1 and UCHL5, altering their DUB activity ( xref )."
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"Moreover, ubiquitinated UCHL5 was indeed enriched in UCHL5 C88A samples but not in USP14 C114A samples from mass spectrometry, which is consistent with that the high molecular weight laddering of UCHL5 represented ubiquitinated species and suggests UCHL5 auto-deubiquitinates itself ( xref )."
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"UCHL5, associated with the 19S regulatory subunit of the 26S proteasome, was ubiquitinated and oxidized in the DB4h sample."
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"Here we report that the human 26S proteasome is ubiquitinated, by which the ubiquitin receptors Adrm1 and S5a, the ATPase subunit Rpt5, and the deubiquitinating enzyme Uch37 are ubiquitinated in situ by proteasome-associating ubiquitination enzymes."
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