IndraLab

Statements

USP10 binds Circulin-C. 28 / 28
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"Furthermore, a subnetwork regulating microglial/macrophage activation that contained circ_0014637 (circ-Usp10), 5 miRNAs (miR-149, miR-152, miR-7033, miR-709 and miR-6963) and 8 target genes (Sbno2, Thbs1Cx3cr1, Cd300a, Il4ra, Syk, Ctsc, Cd84, and Sbno2) was further screened ( xref ))."
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"qRT–PCR, ELISA, flow cytometry, and luciferase reporter assays were carried out to validate the role of circ_0014637 (circ-Usp10) and microRNA(miR)-152-5p /CD84 in microglia."
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"A concurrent decrease in circ-Usp10 expression occurred with elevated expression of miR-152 and a decrease in CD84 in BV2 cells."
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"Nevertheless, the function of circ-Usp10 in SCI is still poorly understood."
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"These results revealed that circ-Usp10 promoted microglial activation and induced neuronal death by targeting miR-152-5p/CD84, which might provide a new potential therapy for SCI."
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"We found that circ-Usp10 inhibited the secretion of proinflammatory factors in microglial BV2 cells."
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"Three siRNAs were designed to influence the expression of circ-Usp10 in microglial BV2 cells stimulated by LPS."
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"The circ-Usp10 may be a diagnostic biomarker and potential target for SCI therapy."
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"To investigate and verify the function of circ-Usp10 in SCI in vitro, we performed a cellular experiment in vitro."
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"Nevertheless, the effect of circ-Usp10 on SCI has not yet been reported."
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"These results suggest that circ-Usp10 may be used as a miR-152 sponge to regulate CD84 expression."
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"We also found that circ-Usp10 regulated the expression of the inflammation-related gene CD84."
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"Circ-Usp10 promotes microglial activation and induces neuronal death by targeting miRNA-152-5p/CD84."
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"These results revealed that circ-Usp10 is a potential biomarker and therapeutic target in SCI."
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"In addition, silencing circ-Usp10 significantly reduced the death of the neuronal cell line HT22."
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"These results indicated that circ-Usp10 promotes microglial/macrophage activation via miR-152/CD84 in microglia."
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"Taken together, we concluded that circ-Usp10 may function as a competing endogenous RNA (ceRNA) to promote microglial activation and induce neuronal death by targeting miR-152-5p/CD84."
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"Supernatants treated with the neuronal cell line HT22 and circ-Usp10 silencing exhibited significantly induced neuronal death ( xref ) and xref ))."
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"Thereafter, we found that circ-Usp10 siRNA-2 significantly inhibited the expression of the proinflammatory factors IL-6 and TNF-α in microglia treated with LPS ( xref ))."
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"As shown in xref ), the expression of miR-152 was significantly enhanced and CD84 was significantly decreased with treatment with circ-Usp10 siRNA-2 in BV2 cells."
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"In addition, circ-Usp10 silencing significantly reduced the death of the neuronal cell line HT22."
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"Hence, we chose circ-Usp10 siRNA-2 for the following experiments."
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"The circ-Usp10 significantly induced neuronal death."
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"In the microglial/macrophage-activated ceRNA subnetwork, our results revealed a concurrent decrease in circ-Usp10 expression with elevated expression of miR-152 in BV2 cells."
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"We also found that circ-Usp10 inhibited the secretion of proinflammatory factors in microglial BV2 cells."
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"Furthermore, a circRNA-mediated ceRNA network was constructed, and a subnetwork including circ_0014637 (circ-Usp10) that regulates microglial/macrophage activation was screened."
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"As shown in xref ), circ-Usp10 siRNA-1 and circ-Usp10 siRNA-2 significantly decreased the expression of circ-Usp10 in microglial BV2 cells compared to the control group."
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"Circ-Usp10 promotes microglial/macrophage activation via miR-152/CD84 in microglia."
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