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USP10 is phosphorylated. 48 / 48
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"However, the relationship between USP10 translocation and stabilization as well as how USP10 phosphorylation affects its stability and localization have not yet been understood."
32512887
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"Comparing to the unphosphorylated USP10, phosphorylated USP10 showed much higher activity towards Ub-AMC ( xref )."
26876938
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"These results suggest that ATM regulates USP10 phosphorylation after DNA damage."
20096447
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"We first phosphorylated USP10 using active AMPK in vitro and checked its activity toward Ub-AMC."
26876938
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"DNA damage promotes ataxia telangiectasia mutated (ATM)-dependent phosphorylation of USP10, which undergoes nuclear translocation."
33919439
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"Therefore, phosphorylated USP10 can inhibit the growth of tumour cells without inducing mutations in p53 ( xref ), which implies that phosphorylation of USP10 has potential therapeutic effects in tumours."
34177595
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"Given USP10 also regulates p53 expression and suppresses tumor cell growth ( Yuan et al., 2010 ), phosphorylation of USP10 under energy stress might contribute to p53 level increase and tumor suppress[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26876938
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"Conversely, MEK-ERK inhibition blocked USP10 phosphorylation and enhanced the USP10-ZEB1 interaction shown to suppress ZEB1-mediated tumor cell migration and metastasis."
36940483
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"We further confirmed the role of ATM in USP10 phosphorylation using ATM +/+ or ATM −/− cells."
20096447
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"Compared to the unphosphorylated USP10, phosphorylated USP10 showed much higher activity toward Ub-AMC ( Figure 5 A)."
26876938
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"As shown in xref and xref , both T42 and S337 sites were phosphorylated following DNA damage and Ku55933 or T42/S337 mutations inhibited USP10 phosphorylation after DNA damage."
20096447
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"Currently, we do not know how the phosphorylation of USP10 affects its stability and localization."
20096447
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"As shown in xref , the phosphorylation of USP10 increased significantly after glucose starvation, suggesting that USP10 might be a substrate of AMPK."
26876938
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"The initial rate of phosphorylation of USP10 peptide was 155±36 pmol/min/reaction mix, which was higher than that of SAMS (95±18 pmol/min/reaction mix) ( xref )."
26876938
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"Furthermore, phosphorylated USP10 can deubiquitinate and stabilize the tumour suppressor protein p53 by reversing its nuclear export and degradation via Mdm2."
34177595
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"These results suggested that the phosphorylation of USP10 is important for its function in AMPK activation and cellular metabolism."
26876938
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"We further examined the functional significance of USP10 phosphorylation by ATM."
20096447
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"To further confirm the phosphorylation of USP10 by AMPK, we compared the phosphorylation of a synthesized USP10 S76 peptide with the SAMS peptide, a known AMPK substrate in an in vitro kinase assay ( [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26876938
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"USP10 phosphorylation by ATM is required for its stabilization and translocation following DNA damage."
20096447
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"Moreover, a special detection with the phospho-(Ser/Thr) Akt substrate antibody confirmed that USP10 phosphorylation was directly related to AKT (Supplementary Fig. 4f) ."
34983926
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"Therefore, phosphorylated USP10 can inhibit the growth of tumour cells without inducing mutations in p53, which implies that phosphorylation of USP10 has a potential therapeutic effect against tumours ( xref ; xref ; xref )."
34177595
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"On the other hand, USP10 is phosphorylated and activated by AMPK under energy stress."
26876938
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"As shown in Figure 4 B, the phosphorylation of USP10 increased significantly after glucose starvation, suggesting that USP10 might be a substrate of AMPK."
26876938
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"Upon DNA damage and ATM activation, USP10 is phosphorylated and accumulates in the nucleus, where it joins forces with USP7 to stabilize p53 via p53 deubiquitination ( Figure 1 , right panel)."
20144755
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"Overall, our results establish the important role of USP10 phosphorylation in p53 activation following DNA damage."
20096447
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"AMPKα AMPKα increases its activity by mediating phosphorylation of Ser76 at the USP10 N-terminus. [15] ATM ATM mediates phosphorylation of USP10 at Thr42 and Ser337 and causes USP10's migration into the nucleus. [27] TRAF4 TRAF4 and p53 competitively bind to USP10 and inhibit usP10-mediated p53 deubiquitination. [73] AKT Co-stimulation by BCR and TLR1/2 initiates Akt-dependent phosphorylation of T674 in the USP10 NLS domain. [72] USP13/beclin-1 When USP10 and USP13 interact with beclin-1, the deubiquitination activity of USP10 can be increased. [53] MiR-138 MiR-138 binds to a conserved region of USP10's 3 -UTR and inhibits the accumulation of USP10 mRNA and protein expression level. [31] MicroRNA-191 MicroRNA-191 binds to the 3 -untranslated region of USP10 mRNA, reducing USP10 protein levels. [28] MiR-34a-5p MiR-34a-5p binds to the 3 -untranslated region of USP10 and reduces the expression of USP10. [79] G3BP Direct binding of G3BP to USP10 inhibits its ability to decompose ubiquitin chains. [56] HTLV-1 Tax The central region of Tax interacts with amino acids 727-798 in USP10, inhibiting the activity of USP10. [78] Daam1 negatively regulates USP10's DUB activity. [80] Estrogen Estrogen induces p53 degradation by regulating USP10 activity. [81] Overexpressed FOXO4 inhibits USP10 transcription and protein expression by binding to the bases 1771-1776 in the promoter region TSS of USP10. [82] Zhang et al. indicated that miR-34a-5p acted as a negative regulator of USP10, but the underlying mechanism of the microRNA's action remains largely unclear [79] ."
35627217
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"More evidence is needed to establish the physiological role of USP10 phosphorylation in tumorigenesis."
32512887
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"Indeed, following various genotoxic stresses (IR, UV or etoposide treatment), USP10 became phosphorylated at SQ/TQ motifs ( xref and xref , USP10 protein levels were equalized to specifically examine USP10 phosphorylation in experiments of xref , xref ), which are consensus phosphorylation sites for PI3-kinase like kinases (PIKKs), such as ATM, ATR, and DNA-PK ( xref )."
20096447
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"Mutation of both T42 and S337 (TS/AA) also abolished USP10 phosphorylation by ATM ( xref )."
20096447
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"In particular, the phosphorylation of USP10 in its NLS domain by BCR-TLR1/2-PI3K-Akt integrates the dual signals from the interaction between TLR and its ligand, or BCR and antigen on the cell surface, facilitating its translocation into the nucleus and transmitting signals into the nucleus to regulate the abundance of AID."
34983926
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"It is possible that USP10 phosphorylation by ATM hinders USP10 nuclear export and shields it from degradation in the cytoplasm."
20096447
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"Both USP7 and USP10 are subjected to phosphorylation-dependent regulation. xref , xref Particularly, phosphorylation of USP7 by the protein kinase CK2 is shown to stabilize USP7. xref Although USP7 or USP10 expression was not altered by acute FSK treatment, any effect on USP7 and USP10 phosphorylation remains to be determined."
33095475
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"In cells reconstituted with USP10 S76A, the phosphorylation of AMPK and ACC1 was much lower than cells reconstituted with WT USP10 ( Figure 5 D), indicating that the phosphorylation of USP10 is import[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
26876938
sparser
"To further confirm the phosphorylation of USP10 by AMPK, we compared the phosphorylation of a synthesized USP10 S76 peptide with the SAMS peptide, a known AMPK substrate in an in vitro kinase assay ( xref )."
26876938
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"Upon DNA damage, Usp10 is phosphorylated which is essential for Usp10 to relocate to the nucleus, allowing it to stabilise p53 [ xref ]."
25962961
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"A double-labeling immunofluorescent study demonstrated colocalization of phosphorylated α-synuclein and USP10 in Lewy bodies ( xref K, 7L, 7N, and 7O), indicating the possible association of both proteins."
30469013
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"Deng et al. revealed that AMPKα directly mediated the phosphorylation of Ser76 at the USP10 N-terminus and increased its activity."
35627217
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"The initial rate of phosphorylation of USP10 peptide was 155 ± 36 pmol/min/reaction mix, which was higher than that of SAMS (95 ± 18 pmol/min/reaction mix) ( Figure 4 D)."
26876938
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"For example, phosphorylated USP10 can deubiquitinate and stabilize p53 by reversing its nuclear export and Mdm2-induced degradation."
34177595
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"Both pan-PIKK inhibitor caffeine and ATM inhibitor Ku55933 inhibited USP10 phosphorylation after DNA damage ( xref )."
20096447
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"Given USP10 also regulates p53 expression and suppresses tumor cell growth ( xref ), phosphorylation of USP10 under energy stress might contribute to p53 level increase and tumor suppression."
26876938
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"DNA damage-induced phosphorylation of USP10 moves it into the nucleus, allowing it to stabilize p53 xref and USP28 stabilizes Chk2 and 53BP1 in response to DNA damage xref ."
32377397
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"We further determined the effect of USP10 phosphorylation on lipogenesis and glycolytic gene expression."
26876938
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"The phosphorylation of USP10 may regulate its activity in a similar manner."
26876938
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"Here we show that phosphorylation of the human USP10 at a single residue, Ser76, is necessary to activate the enzyme."
26876938
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"Here we identified phosphorylated USP10, HDAC2, and β-catenin, which interact with AR."
22699815
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"Moreover, a special detection with the phospho-(Ser/Thr) Akt substrate antibody confirmed that USP10 phosphorylation was directly related to AKT (Supplementary Fig. xref )."
34983926
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"These results suggest that ATM-mediated phosphorylation of USP10 is required for USP10 translocation and stabilization."
20096447