IndraLab

Statements

ESR1 binds PSMD14. 11 / 11
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"Besides, due to the difficulties to acquire purified PSMD14 protein, we failed to perform pulldown assay to show the direct interactions between PSMD14 and ERα."
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"The ChIP-qPCR assay confirmed that ERα depletion resulted in a decrease in ERα binding to the PSMD14 gene (Fig. xref )."
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"Finally, the ChIP-qPCR data indicated that estradiol treatment enhanced ERα binding to the promoter region of PSMD14 in MCF-7 and T47D cells (Fig. xref )."
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"Thus, the further biochemical and structural biology work could be carried out for cocrystal structure of PSMD14-ERα for drug development."
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"PSMD14 associates with ERα in breast cancer cells."
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"The further endogenous immuno-precipitation assay showed that PSMD14 could interact with ERα in MCF-7 cells (Fig. xref )."
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"To analyze the interaction between PSMD14 and ERα, we created deletion constructs."
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"PSMD14 interacted with ERα protein, inhibited ERα poly-ubiquitination and proteasome-dependent degradation in breast cancer cells."
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"The results revealed that the UBD domain of PSMD14 is necessary for its interaction with ERα, while the interaction between PSMD14 and ERα is mediated by the AF1 domain of ERα (Fig. xref )."
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"Since PSMD14 associates with ERα, the biological effect on ERα protein was further investigated."
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"Interestingly, ChIP assay shows that ERα could bind to the promoter region of PSMD14 and facilitate its gene transcription, which indicates PSMD14 is both the upstream modulator and downstream target for ERα signaling in breast cancer."
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