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5-(1H-indol-3-ylmethyl)-3-methyl-2-sulfanylidene-4-imidazolidinone inhibits RIPK1. 191 / 194
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"The role of necroptosis in cardiac I/R injury was first demonstrated using the small molecule inhibitor necrostatin-1 and its optimized analog necrostatin-1 s, which allosterically inhibit RIPK1 kinase activity [54,109]."
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"Inhibition of RIP1 by necrostatin-1 attenuates alcohol-mediated inflammation but not hepatocyte injury ( xref ), while Rip3 − / −mice are protected from chronic ethanol-induced liver injury ( xref , xref )."
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"Necrostatin-1 also prevents neurodegeneration by blocking RIPK1-induced necroptotic and apoptotic pathways induced by Aβ exposure in vitro 69 and in vivo 83–85 and has also shown improved learning and[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"20 Necrostatin-1 and an optimized derivative of necrostatin-1 (7-Cl-O-Nec-1) are small molecules inhibitors of RIP1."
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"Inhibition of RIP-1 by Necrostatin-1 also did not alter cell death in male or female cells (not shown)."
23928697
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"Conversely, inhibition of RIPK1 by necrostatin-1 marginally reduced LDH release from RSV infected macrophages (data not shown)."
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"The LPS or bacterial induced necroptosis in wild type BMDMs was also inhibited by Necrostatin-1 (Nec-1), indicating a RIPK1 kinase dependency (XREF_SUPPLEMENTARY)."
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"Necroptosis is also characterized by the fact that RIPK1 activity can be inhibited by necrostatin-1 (Nec-1) (Degterev et al., 2008; Christofferson and Yuan, 2010)."
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"Necrostatin-1 (Nec-1) is an ATP-competitive small molecule and allosterically inhibits RIP1, an upstream kinase of MLKL (Degterev et al., 2008)."
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"Though Necrostatin-1 has been widely used to inhibit RIPK1 kinase activity, off-target effects have been reported."
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"The pharmacologic small molecule necrostatin 1 inhibits RIPK1 and prevents necroptosis in most cellular models -- a very useful experimental tool."
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"Necrostatin-1 (Nec-1) is an inhibitor of RIP1 [12], which is a key intermediate protein involved in the activation of necroptosis [13]."
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"In addition, it was identified that the increased RIPK1 and RIPK3 can be inhibited by necrostatin-1 in both in vivo and in vitro ."
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"The administration of 7 N-1, an analog of necrostatin-1 which inhibits RIPK1 [ xref ], reduces the density of RIPK1 + oligodendrocytes [ xref ]."
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"The apoptosis component is RIPK1 dependent, which is sensitive to RIPK1 kinase inactivation by necrostatin-1."
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"In the second approach, we turned to a fragment based strategy, combining a hinge binding fragment of ponatinib with a highly selective necrostatin-1 inhibitor of RIPK1."
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"Rotenone induced mitochondrial ROS activate necroptosis via RIP1, which is totally abolished by necrostatin-1."
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"For example, after cerebral ischemia–reperfusion (I/R), necrostatin-1 (an inhibitor of RIP1 [7]) increased the survival rate of hippocampal neurons and attenuated the impairment of learning and memory."
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"Furthermore, in various injury models, Necrostatin-1 (Nec-1) has been shown to inhibit necroptosis by blocking the activity of RIPK1 kinase, suggesting its potential therapeutic role in disease management (Rosenbaum et al., 2010; Kaczmarek et al., 2013)."
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"However, it is certain that Ser161 contributes to necrostatin-1 (Nec-1)-mediated inhibition of RIP1 kinase activity."
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"Inhibition of RIP-1 by Necrostatin-1 (Nec-1) in SW620 cells ameliorated cell death and AIF translocation after AA005 treatment (Figure  xref B, C), whereas AIF knockdown failed to affect the increase in RIP-1 evoked by AA005 (Figure  xref D)."
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"Inhibition of RIPK1 by necrostatin-1 results in decreased cell death and delayed loss of intracellular ATP levels, compared to Z-IETD-FMK or non-treated L929 cells."
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"Additionally, while the effect of Y100 was abrogated by necrostatin-1 (an inhibitor of the necroptosis effector RIPK1), neither siRNA mediated knockdown of RIPK1 nor inhibition of RIPK3 with GSK ' 872 was sufficient to prevent Y100 mediated cell death (XREF_SUPPLEMENTARY and XREF_SUPPLEMENTARY)."
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"This TNF-alpha and Fas-induced cell death is a type of receptor interacting protein-1 (RIP-1)-dependent programmed necrosis called necroptosis, which can be prevented by necrostatin-1, a specific RIP-1 inhibitor."
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"Furthermore, the inhibition of RIPK1 by the necroptosis inhibitor necrostatin-1 (Nec-1) inhibits necroptosis by preventing the formation of complex IIb, suggesting an essential role of RIPK1 in necroptosis [ xref ]."
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"Z-VAD-FMK (an inhibitor of pan-caspase) and necrostatin-1 (a specific inhibitor of RIPK1) are ineffective in blocking ferroptosis ."
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"In addition, necroptosis can be manipulated by inhibitors such as Necrostatin 1, which blocks RIP1 kinase activity."
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"The administration of 7 N-1, an analog of necrostatin-1 which inhibits RIPK1 [300], reduces the density of RIPK1 oligodendrocytes [301]."
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"Necrostatin-1 (Nec-1) inhibits RIP1 and acts as a specific inhibitor of necroptotic cell death [29] ."
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"Treatment with RIPK1-specific inhibitors such as necrostatin-1 (Nec-1), GSK963 and 5-(3′,5 dimethoxybenzal)-2-thio-imidazole-4-ketone (DTIO) also reduced infarct volume and neurological deficit scores and significantly decreased ischemia-induced neuroinflammation and generation of reactive oxygen species (ROS) [15,25–27]."
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"RIPK1 inhibition by necrostatin-1 (10) and MLKL inhibition by necrosulfonamide protect against ischemic brain injury (8,11)."
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"For this, we used necrostatin-1 (Nec1) to inhibit RIPK1, necrosulfonamide (NSA) to inhibit MLKL and siRNA to silence RIPK3."
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"RIPK1 inhibition by necrostatin-1 (10) and MLKL inhibition by necrosulfonamide protects against ischemic brain injury (8,11)."
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"Mechanistically, we show that inactivation of RIP1 by necrostatin-1 suppressed nuclear factor kappaB (NF-kappaB) cascade signals, including activation of IKKalpha, nuclear factor kappa B inhibitor protein alpha (IkappaBalpha), accumulation of nuclear p65 and NF-kappaB promoter activity."
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"Necrostatin-1 (Nec-1) specifically blocks the kinase activity of RIPK1 [XREF_BIBR]."
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"Necrostatin-1 blocks necroptosis in many settings by interacting and inhibiting RIPK1 kinase activity, which is required for activation of RIPK3."
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"In addition, it was identified that the increased RIPK1 and RIPK3 can be inhibited by necrostatin-1 in both in vivo and in vitro."
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"Furthermore, inhibition of RIP1 by Necrostatin-1 has been shown to prevent necroptosis in addition to significantly decreasing intracellular ROS levels."
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"We found that RIP-1 expression was upregulated in the lung of ALI mice and inhibition of RIP-1 by necrostatin-1 significantly prolonged the survival time of mice, which was accompanied by less serve lung injury."
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"We found that RIP-1 expression was upregulated in the lung of ALI mice and inhibition of RIP-1 by necrostatin-1 significantly prolonged the survival time of mice, which was accompanied by less serve lung injury."
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"Necroptosis is another pathway leading to programmed cell death; necroptosis is inhibited by necrostatin-1 which blocks RIP1 kinase, an essential component of the pathway [XREF_BIBR, XREF_BIBR]."
28622381
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"In vitro, human neutrophils fed CA-MRSA lyse by an unknown mechanism that is inhibited by necrostatin-1, an allosteric inhibitor of receptor interacting serine/threonine kinase 1 (RIPK-1)."
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"Necrostatin-1 specifically inhibits RIP1 kinase and disrupts regulated necrosis in tissue culture XREF_BIBR - XREF_BIBR."
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"Necroptosis is a caspase independent cell death and necrostatin-1 inhibits the activity of RIP1 and blocks the necroptosis pathway."
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"Further studies have revealed that Necrostatin-1 specifically inhibits receptor-interacting protein 1 (RIP1)."
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"Further studies have revealed that Necrostatin-1 specifically inhibits receptor-interacting protein 1 (RIP1)."
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"The initial small-molecule inhibitor of RIPK1, necrostatin-1 (Nec-1), was shown to trigger necroptosis in cells."
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"Necroptosis was significantly reduced when RIP1 kinase was inhibited by necrostatin-1, whereas inhibition of caspases with z-vad-fmk, an irreversible pan-caspase inhibitor, did not prevent necroptosis."
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"Necroptosis was significantly reduced when RIP1 kinase was inhibited by necrostatin-1, whereas inhibition of caspases with z-vad-fmk, an irreversible pan-caspase inhibitor, did not prevent necroptosis."
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"Necrostatin-1 specifically inhibits the pro necrotic RIP1 kinase activity, but not the kinase activities of other kinases including RIP2 and RIP3 [XREF_BIBR, XREF_BIBR]."
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"A key inhibitor of RIPK1 kinase in the necroptosis pathway, necrostatin-1 (Nec-1), inhibits RIPK1/RIPK3/MLKL signaling, and thus might inhibit alcohol-induced osteopenia through decreased activation of osteoblast necroptosis (28)."
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"By using a chemical approach, we showed that pretreatment of mice with Necrostatin-1 (Nec-1) (inhibitor of RIPK1) and/or PJ34 (inhibitor of PARP-1) significantly protected mice against concanavalin A (ConA) liver injury (aspartate amino-transferase (AST)/alanine amino-transferase (ALT)) associated with down-regulated hepatocyte specific IL-33 expression."
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"Of note, concomitant inhibition of RIP1 by Necrostatin-1 and caspases by zVAD.fmk cooperated to support cellular survival following radiotherapy [74] ."
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"These results indicate that necrostatin-1 blocks the RIP3 activation by blocking the RIP1 kinase activity, followed by inhibiting necrosome formation in the THLE-2 cells."
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"Here, we investigated whether inhibiting RIPK1 by necrostatin-1 (Nec-1) could limit neuroinflammation and attenuate POCD in D-Galactose (D-Gal)-induced aged mice."
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"Inhibiting RIPK1 by necrostatin-1 strikingly mitigated cognitive impairment and alleviated postoperative amplified neuroinflammation, necroptosis and GluA1 loss in hippocampus."
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"Thus, we here induced aged mice via intraperitoneal injection of D-Gal for 2 months and detected the effects of inhibiting RIPK1 by necrostatin-1 (Nec-1; Yang et al., xref ) on POCD in the aged mice."
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"RIPK1 plays a key role in necroptosis and can be inhibited by necrostatin-1 (Nec-1), which was defined for the first time as a specific inhibitor of necroptosis (11)."
35663937
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"However, RIPK1 can selectively be inhibited by necrostatin-1 (Nec-1), which can significantly reduce necrotic cell death (necroptosis) [13,14]."
32345086
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"Interestingly, inhibition of RIPK3 by GSK’872 reduces necroptotic astrocyte death in Zika virus, whereas necrostatin-1 (an inhibitor of RIPK1) has no protective effect, suggesting that ZIKV-induced necroptosis in astrocytes is independent of RIPK1."
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"Inhibition of RIP1 by necrostatin-1 did not inhibit A1-IRS activity, thereby excluding necroptosis ( xref ). xref We also looked for mitochondrial perturbation and did not observe any cytochrome C redistribution ( xref ) or ROS increase ( xref ) after incubation with A1-IRS, and mitochondria appeared normal when observed under electronic microscopy (data not shown)."
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"Necrostatin-1 blocks the association of RIP1 with RIP3, leading to RIP3 instability, and cleavage from a 57-kDa to a 55-kDa protein."
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"Necrostatin-1 (Nec-1) prevents necroptosis by blocking RIPK1 kinase activity in various injury models (49,50)."
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"Conversely, necroptosis is dependent on RIP1 kinase activity, which can by inhibited by necrostatin-1 (nec-1)."
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"The pan caspase inhibitor zVAD, which inhibits caspase-8, or necrostatin-1s, which inhibits RIPK1, minimally affected RIPK3 activation; in contrast, inhibition of RIPK3 using its inhibitor GSK872 completely removed its phosphorylation ( xref , xref )."
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"For this reason, RIPK1 is often mistaken as the key to necroptosis, and as long as necrostatin-1 (a specific inhibitor for RIPK1) is given to the cells, necroptosis would be impossible to happen."
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"Inhibition of RIP-1 by Necrostatin-1 (Nec-1) in SW620 cells ameliorated cell death and AIF translocation after AA005 treatment, whereas AIF knockdown failed to affect the increase in RIP-1 evoked by AA005."
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"Recently, receptor interacting protein-1 (RIP1)-dependent necrosis, which is inhibited by necrostatin-1, an inhibitor of RIP1, has emerged as a form of regulated necrosis."
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"Inhibition of RIP1 by necrostatin-1 clearly protected from TNF induced necroptosis in all three L929 sublines, but had no effect on the course of MNNG induced necrosis over 16h."
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"It is possible that LPS priming (which contributes to ripoptosome activation) induces RIP1 kinase activity, and that this pool of “preactivated” RIP1 is not inhibited by subsequent Necrostatin-1 and S[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Upregulation of the inflammatory response during necroptosis stimulation by IFNβ+zVAD treatment was significantly reduced in cells treated with Necrostatin-1 (Nec-1), which blocks the kinase activity of RipK1 and inhibits RipK1-RipK3 interaction [28] (Figs."
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"Moreover, RIP1 inhibition by necrostatin-1 only reduces mitochondrial but not cytosolic ROS elevation (Fig.  xref )."
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"Necrostatin-1 (Nec-1) selectively inhibits RIPK1 in various pathological conditions."
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"The assembly of necrosomes requires the kinase activity of RIPK1, which is inhibited by necrostatin-1; this inhibitory effect is specific to necroptosis (104, 105)."
38164133
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"Moreover, it is crucial to highlight that Necrostatin-1 is a specific inhibitor of RIPK1."
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"Inhibition of RIPK1 by necrostatin-1 or RIPK1-siRNA alone did not influence the proliferation of HCC, so the synergism between pirarubicin and RIPK1 inhibition is dominated by pirarubicin (Figure 1D)."
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"Inhibition of necroptosis effectors RIPK1 by the small molecule inhibitor Necrostatin-1 was sufficient to rescue multiple CRC cell lines from ddC, AZA, and combination ddC+AZA mediated toxicity ( xref )."
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"XREF_BIBR and XREF_BIBR Although inhibition of necroptosis by RIP3 deficiency, RIP1 blockade by necrostatin-1, or MLKL deficiency all reduce cell death at early time points, XREF_BIBR and XREF_BIBR RIP3 deficiency was unable to abrogate acetaminophen induced liver injury at 24 hours."
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"It was reported that necrostatin-1 inhibited the increase of p-AKT , confirming that phosphorylation of AKT in response to TNFa/zVAD were dependent on RIPK1 in necroptosis 19,20."
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"Necrostatin-1 (A selective inhibitor of RIPK1) and PD98059 (an ERK1/2 inhibitor) were procured from Sigma-Aldrich and dissolved in 20% DMSO."
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"Furthermore, necrostatin-1, which inhibits RIPK1, a central regulator of necroptosis ( xref ), did not improve cell viability after combination treatment with lapatinib and FTY720, although necrostatin-1 significantly improved cell viability after gefitinib treatment under starvation conditions that induce necroptosis. ( xref and xref )."
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"This led to the identification of necrostatin-1 (Nec-1), which was shown to inhibit RIPK1."
29598451
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"Furthermore, inhibition of RIP1 by Necrostatin-1 has been shown to prevent necroptosis in addition to significantly decreasing intracellular ROS levels."
39611918
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"Necrostatin-1, as an inhibitor of receptor interacting protein 1 kinase, inhibits receptor interacting protein 1 kinase activity and plays an anti-inflammatory role."
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"We used Z-VAD as a general caspase inhibitor to inhibit apoptosis, necrostatin-1 to inhibit RIP1, CRID3, and Y-VAD to inhibit the inflammasome capacity, and we found that the inhibition of caspases and RIP1 did not affect the cell mortality in infected cells."
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"Necrostatin-1 blocks both RIPK1 and IDO : consequences for the study of cell death in experimental disease models."
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"Necrostatin-1 inhibits receptor-interacting serine/threonine-protein kinase 1 (RIPK1) to initiate necroptosis; it also inhibits indoleamine 2,3-dioxygenase (IDO) to regulate tumor immunity."
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"While the expression of RIP1 in mouse liver tissues had no change following ethanol feeding, and inhibition of RIP1 kinase by necrostatin-1 did not mitigate ethanol-induced hepatocyte injury [ xref ]."
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"Necroptosis is a well-regulated mechanism inhibited by Necrostatin-1 (Nec-1), which abrogates the kinase activity of RIPK1 [47]."
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"These suggested that necrostatin-1 limited the upregulation of RIP1 and NF-κB induced by chronic stress in D-galactose-induced aging mice."
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"9 Generation of the kinase receptor interacting protein 1 (RIP1)/RIP3 containing ' necrosomes ' activates downstream necroptotic signaling, which can be inhibited by necrostatin 1 (XREF_FIG)."
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"Timing dependent, necrostatin-1 diminished RIPK1 (P < 0.001), and aborted the necroptosis induced renal cell injury."
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"In the other hand, the inhibition of RIPK1 by necrostatin-1 is still controversial: protective xref and deleterious xref , xref roles have been reported."
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"These results show that degradation of cIAP1/2 by LCL161 and inactivation of RIPK1 by necrostatin-1 results in death of normal macrophages and suggest that cIAP1/2 and RIPK1 play an important role in regulating viability of human macrophages."
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"Of note, researches have demonstrated that necrostatin-1 (Nec-1), a small molecule inhibitor of necroptosis, can effectively impede RIPK1 kinase activity and mitigate the associated inflammatory response [11]."
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"Generally, necroptosis is defined as cell death mediated through a pathway that depends on the RIPK1-RIPK3 complex that may be inhibited by necrostatin-1 (Nec-1) ( xref )."
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"Necrostatin-1 inhibits RIPK1 by blocking its association with RIP3."
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"Since necrostatin-1 has been shown to block not only RIPK1 but also indoleamine 2,3-dioxygenase (IDO), the IDO2 specific inhibitor 1-methyl-D-tryptophan (1-MT) was added to BMDC cultures as a control."
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"The addition of necrostatin-1 inhibited the cell death induced by T+S+V in both the mock and VZV infected populations, confirming the involvement of RIPK1 in this cell death pathway."
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"Necrostatin-1 (Nec-1) is an ATP-competitive small molecule and allosterically inhibits RIP1, an upstream kinase of MLKL ( xref )."
37601059
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"The necrostatin-1 inhibits RIP1 kinase activity, which is required to trigger necroptosis pathway."
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"In an OS model, RIPK1 mediated necroptosis was confirmed as the main cell death mechanism involved in Shikonin therapy, as only Necrostatin-1 (an inhibitor of RIPK1) was able to induce treatment reversion."
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"The assembly of necrosomes requires the kinase activity of RIPK1, which is inhibited by necrostatin-1; this inhibitory effect is specific to necroptosis ( xref , xref )."
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"Necrostatin-1, which suppresses necroptosis by inhibiting receptor-interacting protein kinase 1 (RIP1), significantly and partly reduced the necrotic/necroptotic DRG neurons (Figure 1B), suggesting that methylmercury causes necrosis, necroptosis, and apoptosis."
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"The apoptosis component is RIPK1 dependent, which is sensitive to RIPK1 kinase inactivation by necrostatin-1."
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"It was used different inhibitors of cell death : Z-VAD, a pan-caspase inhibitor that blocks apoptosis; Necrostatin-1, which inhibits RIPK1 activity and blocks necroptosis; and the iron chelator, deferoxamine, that chelates iron and prevents ferroptosis."
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"Necrostatin-1 specifically inhibits RIP1 kinase and disrupts regulated necrosis in tissue culture xref – xref ."
23145018
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"Inhibition of RIP-1 by Necrostatin-1 also did not alter cell death in male or female cells (not shown)."
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"In line with its essential role in programmed necrosis, pharmacological inhibition of RIPK1 by necrostatin-1 protected the same subset of sensitive tumor cell lines that we had used for analysis in Figure  xref d (resistant KNS-62 cells were again included as control) from both TRAIL/zVAD/CHX- and TNF/zVAD/CHX-induced programmed necrosis (Figure  xref a)."
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"Inhibition of RIPK1 by necrostatin-1 or RIPK1-siRNA alone did not influence the proliferation of HCC, so the synergism between pirarubicin and RIPK1 inhibition is dominated by pirarubicin (Figure xref D)."
30588188
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"Necrostatin-1 inhibits the activity of receptor-interacting protein kinase 1 (RIPK1), highlighting the activation of the serine/threonine kinase RIPK1 as an important key molecular step within the activation cascade of necroptosis [101,102,103,104]."
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"RIP1, but not its kinase activity, functions as a scaffold in the survival-promoting activity mediated by complex I. Inhibition of RIP1 kinase by necrostatin-1 (Nec-1) has no effect on the activation [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"In contrast, RIP1 expression in mouse liver remained unchanged following ethanol feeding and inhibition of RIP1 kinase by necrostatin-1 did not attenuate ethanol-induced hepatocyte injury."
23319235
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"Necrostatin-1 (an inhibitor of RIPK1, RIPK3-KO, and MLKL-KO) mice were recently used to demonstrate the role of necroptosis in IR- and cisplatin induced AKI."
26924060
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"Moreover, RIP1 inhibition by necrostatin-1 only reduces mitochondrial but not cytosolic ROS elevation."
26381601
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"Furthermore, in various injury models, Necrostatin-1 (Nec-1) has been shown to inhibit RIPK1 kinase activity, thereby suppressing necroptosis (61, 62)."
39544947
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"In particular, necrostatin-1 has been identified to specifically inhibit the kinase activity of RIP1, thereby undermining its interaction with RIP3 and antagonizing necroptosis, without affecting NF-kappaB XREF_BIBR."
24714696
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"Functional analysis showed that the inhibition of RIP1 caused by necrostatin-1 (Nec-1) inhibited the proliferation, simultaneously facilitate both the apoptosis and differentiation of AML cells."
35370472
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"RIPK1 can be regulated and pharmacologically inhibited by necrostatin-1 (Nec-1)."
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"6 Necrostatin-1 (Nec-1) prevents necroptosis by binding to and inactivating RIP1."
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"In contrast, necroptosis inhibitors necrosulfonamide (NSA) and necrostatin-1s (Nec-1s) which inhibits MLKL and RIPK1 respectively, partially (to 68–72% of control cell viability) prevented FAC and erastin-induced cell death in the IB3-1 CF cells (Fig.  xref A)."
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"Previous studies have reported that pharmacological inhibition of RIPK1 by necrostatin-1 (Nec-1) or Nec-1 stable (Nec-1s) showed neuroprotective effects in 6-hydroxydopamine (OHDA)-treated PC12 cells, neural cells derived from induced pluripotent stem cells of PD patients with OPA1 gene mutations, and in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD mice ."
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"Notably, Previous studies have revealed that RIPK1 expression in mouse liver remains unchanged after ethanol feeding, and inhibition of RIP1 kinase by necrostatin-1 does not alleviate ethanol-induced hepatocyte injury ( xref )."
39669199
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"Indeed, inhibition of RIP1 by necrostatin-1 abolishes both apoptosis and necrosis in RPE cells."
28615451
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"Necrostatin-1 (Nec-1) inhibits receptor-interacting protein 1 (RIP1) kinase and programmed necrosis."
24198446
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"Indeed, inhibition of RIP1 by necrostatin-1 abolishes both apoptosis and necrosis in RPE cells."
28615451
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"Necrostatin-1 (Nec-1) is a potent inhibitor for RIPK1, which demonstrates benefits to the brain during cerebral ischemia and I/R injury such as reductions in infarct volume and neurological deficits [[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"However, it should be noted that, although inhibition of RIP1 kinase by necrostatin-1 did not have an effect on the cytotoxic activity of NaD1, the ability of NaD1 to effect the necroptotic pathway downstream of RIP1 kinase activity, such as via direct modulation of MLKL-mediated membrane disruption, xref not addressed in our current study should be determined to eliminate definitively necroptosis as having a role."
28179997
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"Necrostatin-1 inhibits the kinase activity of RIP1 26 while necrosulfonamide targets MLKL, which has recently emerged as a key signaling protein that mediates binding to downstream targets."
23985697
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"In line with its essential role in programmed necrosis, pharmacological inhibition of RIPK1 by necrostatin-1 protected the same subset of sensitive tumor cell lines that we had used for analysis in Figure XREF_FIG d (resistant KNS-62 cells were again included as control) from both TRAIL/zVAD/CHX- and TNF/zVAD/CHX induced programmed necrosis."
24507727
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"Inhibition of RIP1 by Necrostatin-1 and even more effectively p38-MAPK inhibition by the p38 inhibitor III rescued cells from imatinib deprivation induced cell death (XREF_FIG, right panel), indicating that these proteins play a major role for cell death upon oncogenic stress."
21949869
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"Further, Necrostatin-1 (Nec-1) -- an inhibitor of RIP1 kinase activity -- dramatically increased baculoviral transgene expression in RIP1 silenced cells."
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"Previous studies have reported that pharmacological inhibition of RIPK1 by necrostatin-1 (Nec-1) or Nec-1 stable (Nec-1s) showed neuroprotective effects in 6-hydroxydopamine (OHDA)-treated PC12 cells, neural cells derived from induced pluripotent stem cells of PD patients with OPA1 gene mutations, and in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD mice xref , xref ."
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"This is consistent with the notion that Necrostatin-1 inhibits RIPK1 kinase activity, which is required for the formation of RIPK1/FADD/caspase -8 complex (Degterev et al., 2008; Wang et al., 2008)."
19524512
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"Necrostatin-1 (Nec-1), a small molecule compound, inhibits the activity of RIP1 and is a specific inhibitor of necroptosis (Degterev et al., 2005)."
35662218
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"Necrostatin-1 administration inhibited the upregulation of RIP1 and RIP3 and enhanced the expression of caspase-8 in DSS induced colitis."
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"RIPK1 inhibition by necrostatin-1 ( xref ) and MLKL inhibition by necrosulfonamide protect against ischemic brain injury ( xref , xref )."
30394012
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"RIPK1 inhibition by necrostatin-1 ( xref ) and MLKL inhibition by necrosulfonamide protects against ischemic brain injury ( xref , xref )."
30394012
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"We have previously demonstrated the role of microglial necroptosis in retinal degeneration and neuroinflammation, focusing on TLR signaling and the specific inhibition of RIP1 by necrostatin-1 [47]."
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"26 Here, we show that LTX-315 also can induce ICD in the context of a necrotic pathway that is independent of RIP3 (and is not inhibited by necrostatin 1, which inhibits RIP1), XREF_BIBR, XREF_BIBR suggesting that different pathways leading to necrosis can cause ICD."
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"Inhibition of RIP1 by necrostatin-1 did not inhibit A1-IRS activity, thereby excluding necroptosis (XREF_SUPPLEMENTARY)."
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"Necrostatin-1 treatment abrogated association of RIPK1 and MLKL with ATG5 (XREF_FIG), indicating a requirement for RIPK1 kinase activity for complex formation."
27219062
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"The RIP1 antagonist necrostatin-1 prevented the loss of ES-MNs exposed to either mutSOD1 or sALS (XREF_FIG) astrocytes."
24508385
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"Inhibition of RIPK1 by Necrostatin-1s (Nec1s) resulted in reduced, but not totally inhibited, caspase activation and cell death, demonstrating simultaneous occurrence of RIPK1 kinase-dependent and -independent apoptosis (Fig. xref )."
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"Inhibition of RIP1 by necrostatin 1 partially inhibited TNF-α/ZVAD-induced necrosis."
27616656
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"Inhibition of RIP1 by Necrostatin-1 and even more effectively p38-MAPK inhibition by the p38 inhibitor III rescued cells from imatinib deprivation induced cell death ( xref , right panel), indicating that these proteins play a major role for cell death upon oncogenic stress."
21949869
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"Necrostatin-1 inhibited RIP1 dependent necrosis in murine hearts, leading to infarct size reduction and preservation of cardiac function in magnetic resonance imaging (MRI) scans 28 days after I/R injury [XREF_BIBR]."
| PMC
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"In rodents, it has previously been shown that necrostatin-1 (Nec-1) inhibits RIP1, a central regulator of programmed necrosis, thereby decreasing cell death and reducing infarct size (IS) after ischaemia and reperfusion (I/R) injury."
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"Necrostatin-1 pretreatment markedly reduced IR-induced muscle damage and RIPK1, Bok, and cleaved caspase-3 expression, whereas upregualted Bcl-2 expression (p < 0.05)."
36599139
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"The first stage of the work was to study the effect of RIPK1 inhibition by necrostatin-1 under physiological conditions."
35054920
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"Inhibition of RIP1 by Necrostatin-1 partially rescued cells from imatinib deprivation-induced cell death, indicating that this necrotic/necroptotic signaling pathway substitutes for the blocked apoptosis at least partially."
21949869
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"RIPK1 can be inhibited by Necrostatin-1, and, with higher affinity by 7-Cl-O-Nec-1 (Nec-1s), which is also suitable for animal models. xref Following treatment ofFmr1 null mice with Nec-1s, we observed reduced phosphorylation of RIPK1 ( xref )."
31409605
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"Interestingly, Necrostatin-1 which inhibits activation of Receptor Interacting Protein 1 (RIP1), a key mediator of necroptosis, can significantly improve the cryopreservation of spermatogonial stem cells [57]."
| PMC
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"Addition of necrostatin-1 to block RIPK1 kinase also partially inhibited cell death induced by the two IKK inhibitors (Fig. 4a) with a modest effect on Caspase-8, Caspase-3, and PARP cleavage (Fig. 4b)."
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"S. aureus can also trigger necroptosis, cell death dependent on RIPK1, RIPK3, and MLKL that can be blocked by necrostatin-1 and necrosulfonamide (NSA)."
27460814
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"Furthermore, heme mediated cell death is unaffected by the addition of necrostatin-1 (a specific inhibitor of RIP1) (results not shown)."
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"We found that RIP1 inhibition by necrostatin-1 or necrostatin-1s prevented oxytosis in HT-22 cells (Fig. 4g, Supplementary Fig. S3b, c), and depletion of either RIP1 or RIP3 using siRNA or geldanamycin ameliorated glutamate toxicity."
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"Necroptosis has been reported to drive systemic inflammatory response syndrome (SIRS) [131], while necroptosis inhibition by genetic means (e.g., deletion in RIPK3 gene) or by pharmacological means (RIPK1 inhibition by necrostatin-1) protects against SIRS [131]."
36611990
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"Z-VAD-FMK (a caspase inhibitor) and necrostatin-1 (a specific inhibitor of RIPK1) were utilized to determine whether the cell death was mediated by RIPK1 or caspases."
29760660
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"Inhibition of RIP1 by Necrostatin-1 partially rescued cells from imatinib deprivation induced cell death, indicating that this necrotic and necroptotic signaling pathway substitutes for the blocked apoptosis at least partially."
21949869
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"Moreover, necrostatin-1, an inhibitor that targets RIP-1, has been shown to exert neuroprotective functions and to shield neurons from cell death following CCI-TBI."
22437344
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"Necrostatin-1 (Nec-1) inhibits receptor interacting protein 1 (RIP1) kinase and programmed necrosis."
24198446
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"Of note, concomitant inhibition of RIP1 by Necrostatin-1 and caspases by zVAD.fmk cooperated to support cellular survival following radiotherapy [74] ."
25065969
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"Motor neuron survival could be rescued by inhibition of RIP1 by either necrostatin-1 or RIP-1 shRNA."
26505672
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"We also included necrostatin-1, which inhibits RIP1 kinase activity and therefore necroptosis."
27302486
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"Necroptosis induction requires the activity of RIP-1 kinase, which can be repressed by necrostatin-1 (Nec-1)."
25725104
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"Necroptosis has been reported to drive systemic inflammatory response syndrome (SIRS) [ xref ], while necroptosis inhibition by genetic means (e.g., deletion in RIPK3 gene) or by pharmacological means (RIPK1 inhibition by necrostatin-1) protects against SIRS [ xref ]."
36611990
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"Importantly, treatment with necrostatin-1 (nec-1) (78) ( Figure S4 ) or GSK-872 (79) ( Figure 5F ), inhibitors of RIPK1 and RIPK3, respectively, reversed the effects of FAK inhibition and restored cell viability to levels comparable to control macrophages."
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"Furthermore, necrostatin-1, which inhibits RIPK1, a central regulator of necroptosis (47), did not improve cell viability after combination treatment with lapatinib and FTY720, although necrostatin-1 significantly improved cell viability after gefitinib treatment under starvation conditions that induce necroptosis."
34958115
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"In a previous study, we showed that expression of the tnt gene in Jurkat T cells (Jurkat 655-TNT) induced a necrotic like cell death that was not prevented by necrostatin-1, indicating RIPK1 independent necrosis."
29996103
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"Further studies have revealed that Necrostatin-1 specifically inhibits receptor-interacting protein 1 (RIP1). xref investigated the pharmacokinetics and bioavailability of Necrostatin-1 using an LC–MS/MS method, reporting an absolute bioavailability of 54.8%."
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"We have previously demonstrated the role of microglial necroptosis in retinal degeneration and neuroinflammation, focusing on TLR signaling and the specific inhibition of RIP1 by necrostatin-1 [ xref ]."
36991017
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"Necrostatin-1 (Nec-1) inhibits RIP1 and acts as a specific inhibitor of necroptotic cell death [29] ."
21669190
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"To confirm that the cell death observed in MIA40C55S cells is AIFM1-dependent, the cells were treated with either Necrostatin-1 or DPQ, inhibitors of RIP1 and PARP-1 respectively."
40055465
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"Furthermore, we tested an inactive analog of necrostatin-1, necrostatin-1I, which does not inhibit RIPK1 or necroptosis in other settings."
23386613
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"Elevated RIPK3 levels in plasma were associated with ARDS in septic patients, and inhibition of RIPK1 by necrostatin-1 decreased lung injury in neonatal septic mice [42, 43]."
39856779
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"Necrostatin-1 (Nec-1) has been used in various solid organ transplantation models to block RIPK1 activity [20,23,24,40,42,43,45]."
37759518
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"We further explored whether necrostatin-1 treatment could inhibit RIPK1 kinase and investigated its effects on the downstream signaling pathway of RIPK1 with EtOH intervention."
34745415
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"The RIPK1 antagonist necrostatin-1 prevented this motoneuronal loss [38], thus the authors concluded that ALS astrocytes induced motoneuronal necroptosis in their model."
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"64 Indeed, genetic deletion of RIPK3 in Casp8 -/- dendritic cells rescues LPS induced IL-1beta production from Casp8 -/- dendritic cells, and necrostatin-1 (a chemical inhibitor of RIPK1 kinase activi[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
25451151
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"To determine if necroptosis was involved in the neurotoxicity induced by miR-21, we treated the cultures with necrostatin-1, which specifically inhibits RIPK-1."
26154133
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"A necrostatin-1 dose response for the concentration of necrostatin-1 required to inhibit the RIP1 activity in U1 cells was determined by EGFRvIII driven phosphorylation of phospho-p65 (XREF_SUPPLEMENTARY)."
23972990
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"Inhibition of RIPK1 by necrostatin-1 results in decreased cell death and delayed loss of intracellular ATP levels, compared to Z-IETD-FMK or non-treated L929 cells."
36768641
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"Another study showed that targeting necroptosis signalling with the specific RIPK1 antagonist Necrostatin-1 or genetic deletion of Mlkl systemically in vivo, partially protected mice from inferior vena cava ligation induced venous thrombosis 62."
33510145
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"In 2008, Degterev et al. (2008) reported that necrostatin-1 inhibits RIPK1 kinase activity."
32320653
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"It can be inhibited either with Necrostatin-1 (Nec-1), by blocking the activity of the RIPK1 receptor, or with GSK872 which inhibits RIPK3 [261,262]."
| PMC
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"Necrostatin-1 (Nec-1) selectively inhibits RIPK1 in various pathological conditions."
39465486
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"Accordingly, inhibition of RIP1 by the pharmacological inhibitor Necrostatin-1 or by genetic knockdown resulted in inhibition of caspase activation and apoptosis upon combined treatment with Smac mime[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
25550219
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"Necrostatin-1 (Nec-1, a specific inhibitor of RIP1) can inhibit RIP1 kinase activity and stop necroptosis [8] ."
25907058
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"ZVAD-FMK (an inhibitor of pan-caspase), necrostatin-1 (a selective inhibitor of RIPK1), and chloroquine (an inhibitor of autophagy by raising the lysosomal pH) can not block ferroptosis [XREF_BIBR]."
29038744
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"Inhibition of RIP1 by necrostatin-1 clearly protected from TNF-induced necroptosis in all three L929 sublines, but had no effect on the course of MNNG-induced necrosis over 16 h (Fig.  xref b)."
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