IndraLab

Statements

EGF leads to the phosphorylation of STAT1 on S727. 9 / 9
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"Although EGF and IFNγ both phosphorylate Stat1 at S727, the difference is that IFNγ requires phosphorylation at tyrosine701 (Y701) before the S727 phosphorylation event, xref whereas EGF is able to directly phosphorylate Stat1 at S727 via EGFR. xref In detail, immunoblot analysis of lysates from MPHs treated with EGF or IFNγ for different time intervals displayed that IFNγ induced phosphorylation of Stat1 at Y701 within 5 min, which was followed by phosphorylation of S727 at between 10 and 30 min ( xref B)."
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"Indeed, we found that both EGF and IFN-γ strongly activated Stat1 phosphorylation at both Ser727 and Tyr701 in U87-EGFR cells."
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"Indeed, we found that both EGF and IFN-gamma strongly activated Stat1 phosphorylation at both Ser727 and Tyr701 in U87-EGFR cells."
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"It was observed that inhibition of ERK½ by U0126 in HTR-8/SVneo cells resulted in concomitant inhibition of EGF-induced increase in STAT1 and STAT3 phosphorylation at ser 727 along with inhibition of degradation of total STAT1 (Figs xref and xref )."
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"The effects of different cytokines on STAT1 S727 phosphorylation in WT and dKO 293 cells are shown in xref D. While the tested cytokines had little effect in the WT cells, the lower basal STAT1 S727 phosphorylation in the dKO cells (lacking CDK8/19) was increased by EGF, TNFα and serum. xref E shows that CDK8/19-independent STAT1 S727 phosphorylation in dKO 293 cells was also increased by cytotoxic drugs etoposide and Taxol; the time course of the induction of CDK8/19-independent STAT1 S727 phosphorylation by 50 nM Taxol in dKO 293 cells is shown in xref F."
31717492
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"Based on three lines of evidence, we elucidated that IFNγ- and EGF-induced STAT1 Ser727 phosphorylation are mechanistically independent events, as follows. (1) Tyr701 phosphorylation of STAT1 is necessary for IFNγ-induced STAT1 Ser727 phosphorylation. xref (2) EGF induced Ser727 phosphorylation alone without Tyr701 phosphorylation, xref which is consistent with our results ( xref B). (3) EGF treatment enhanced STAT1 binding to the Ecm1 promoter ( xref H, I; xref ), whereas IFNγ treatment impeded the EGF-promoted binding ( xref C, D)."
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"Both EGF and IFN-gamma are able to cause phosphorylation of Stat1 at both Tyr701 and Ser727 for its full activation."
22162832
sparser
"Both EGF and IFN-γ are able to cause phosphorylation of Stat1 at both Tyr701 and Ser727 for its full activation."
22162832
reach
"While the tested cytokines had little effect in the WT cells, the lower basal STAT1 S727 phosphorylation in the dKO cells (lacking CDK8/19) was increased by EGF, TNFα and serum."
31717492