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EGF phosphorylates MAPK1. 65 / 73
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"Third, preincubation of cells with the specific MEK1 inhibitor PD098059 attenuated the phosphorylation of ERK2 and ERK1 by EGF."
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"Both EGF- and NGF induced phosphorylation of ERK2 in PC12 cells, as manifested by a shift in the electrophoretic mobility of ERK2 [10,11]."
10913625
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"Here we report that expression of p21rasAsn-17 does not abolish epidermal growth factor (EGF)-induced phosphorylation of ERK2 in fibroblasts."
8246947
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"50 Since EGF induces phosphorylation of ERK1 and ERK2 in U1242 MG cells 37 and the phosphorylation of mitogen-activated protein kinase (MAPK/ERK) increases in glioblastoma specimens compared with no[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
20413683
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"In rat-1 cells, inhibition of either PKC, by prolonged 12-O-tetradecanoylphorbol-13-acetate (TPA) pretreatment, or calcium influx, by ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) pretreatment, did not abolish EGF-induced ERK2 phosphorylation."
8246947
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"However, a combined inhibition of both p21ras and calcium influx, but not PKC, resulted in a complete inhibition of EGF-induced ERK2 phosphorylation."
8246947
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"In addition, it was shown that epidermal growth factor, which induces the MAP kinase cascade in NIH 3T3 cells, also activated endogenous Erk1 and Erk2 and NF-M tail domain phosphorylation in the transfected cells."
10231383
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"In contrast, in Swiss 3T3 cells, inhibition of both p21ras activation and TPA-sensitive PKC, but not calcium influx, inhibited EGF-induced ERK2 phosphorylation."
8246947
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"We found that substitution of residues 173-175 and particularly Pro(174) to alanines reduces the EGF-induced ERK2 phosphorylation, without modifying its in vitro phosphorylation by MEK1."
17597065
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"ERK1 and ERK2 phosphorylation and activity were initiated by addition of exogenous epidermal growth factor (EGF) and transforming growth factor-alpha, but not by isoforms of platelet derived growth factor or insulin like growth factor-1 in mesothelial cells."
8968079
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"Epidermal growth factor induces phosphorylation of extracellular signal-regulated kinase 2 via multiple pathways."
7888635
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"Also, transient upregulation of ATIP1 in cells stably expressing AT2 receptor has been shown to lessen EGF-induced ERK2 phosphorylation compared to wild type cells transfected with ATIP1 ( Nouet et al[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
28499941
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"Interestingly, EGF causes phosphorylation of ERK2 only in K-Ras G12D mutated PDECs (XREF_SUPPLEMENTARY)."
22160930
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"50 Since EGF induces phosphorylation of ERK1 and ERK2 in U1242 MG cells 37 and the phosphorylation of mitogen activated protein kinase (MAPK and ERK) increases in glioblastoma specimens compared with [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
20413683
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"Expression of wild-type Dok-R in COS1 cells resulted in a marked reduction in phosphorylation of Erk2 following EGF stimulation when compared with cells expressing the vector control."
10508618
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"Epidermal growth factor induces phosphorylation of extracellular signal-regu- lated kinase 2 via multiple pathways."
7781062
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"Moreover, HRas silencing reduced EGF induced ERK1 and ERK2 phosphorylation, supporting a role of HRas in EGF induced MAPK activation in hepatocytes."
23498955
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"Nevertheless, this effect cannot be generalized for all growth factor signaling pathways since first, a dominant-negative Raf mutant does not inhibit the EGF- or TPA-induced MAPK activation although t[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
7888635
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"EGF stimulates phosphorylation of mitogen-activated protein kinase 1 (ERK2), thereby altering its structure and making Cys65 solvent-exposed."
37248656
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"For instance, both EGF and TGFα directly induce phosphorylation of ERK1 and ERK2 in cultured granulosa cells, and the loss of MAP kinase signaling has been associated with granulosa cell apoptosis fol[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12818644
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"Other recent studies show that blockade of endocytosis of EGF receptors by expression of mutant dynamin causes inhibition of EGF-dependent phosphorylation of ERK1, ERK2, and PI3K ( xref )."
9182681
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"EGF treatment promoted phosphorylation and activation of Erk1 and Erk2 in cultured astrocytes (XREF_FIG), as expected because the Ras-MAP kinase pathway is a major pathway known to be activated by the EGF receptor."
19176818
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"Conversely, immunoblot analyses of whole-cell extracts of these cells indicated that EGF-induced phosphorylation of extracellular signal-regulated kinase proteins, ERK1 and ERK2, was not affected by TGFbeta."
12880367
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"Epidermal growth factor induces phosphorylation of extracellular signal regulated kinase 2 via multiple pathways."
8246947
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"Epidermal growth factor induces phosphorylation of extracellular signal regulated kinase 2 via multiple pathways."
20126471
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"Phosphorylation of both ERK1 and ERK2 by EGF was apparent after 5 min and had not diminished by 30 min in the vehicle treated cells."
15642133
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"Similar to what was shown when endogenous Erks were examined, the late phosphorylation of GFP-Erk2 was stimulated by NGF, but not EGF."
12706116
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"Incubation of serum-starved Swiss 3T3 cells with LT prevents the epidermal growth factor-induced phosphorylation of mitogen-activated protein kinases ERK1 and ERK2, indicating that the toxin blocks Ras function in vivo."
8626586
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"Epidermal growth factor induces phosphorylation of extracellular signal–regulated kinase 2 via multiple pathways."
20126471
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"For instance, both EGF and TGFα directly induce phosphorylation of ERK1 and ERK2 in cultured granulosa cells, and the loss of MAP kinase signaling has been associated with granulosa cell apoptosis fol[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12818644
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"Epidermal growth factor (EGF), which has been shown to enhance the phosphorylation of ERK1 and ERK2 [10] , was used as a positive control (Fig. 5) ."
15177938
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"Western blot analysis with the active MAPK antibody showed that treatment with EGF and PMA both stimulated the phosphorylation of ERK2 and ERK1."
12644307
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"C., T ertoolen, L. G. J. and Bos, J. L., 1993, Epidermal growth factor induces phosphorylation of extracellular signal regulated kinase 2 via multiple pathways."
11258851
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"In keeping with changes in ERK2 activity ( Figure 3 ), EGF induced tyrosine phosphorylation of ERK2 in parental GECs adherent to collagen, but there was little change in cells on plastic ( Figure 7A [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
10079268
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"Burgering B., De Vries-Smits A. M., Medema R. H., Van Weeren P. C., Tertoolen L. J. and Bos J. L. (1993) Epidermal growth factor induces phosphorylation of extracellular signal-regulated kinase 2 via[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
8649450
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"EGF significantly ( P <0.01) stimulated Elk-1 phosphorylation in both ERK2 and ERK1 immunoprecipitates."
12644307
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"In pcDNA3-transfected PC3 cells (negative control), 10 ng/ml EGF significantly increased ERK2 phosphorylation ( P < 0.05) (represented by an increase in phospho-ERK2 in xref )."
20687230
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"Phosphorylation of both ERK1 and ERK2 by EGF was apparent after 5 min and had not diminished by 30 min in the vehicle-treated cells."
15642133
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"Treatment of these cells with the MEK1 inhibitor blocked the EGF-induced migration of these cells and selectively prevented EGF-induced ERK1 and ERK2 phosphorylation without impacting other EGFdependent phosphorylation events, including the autophosphorylation of the EGF receptor (data not shown)."
9128257
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"Third, preincubation of cells with the specific MEK1 inhibitor PD098059 attenuated the phosphorylation of ERK2 and ERK1 by EGF."
12644307
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"Treatment of these cells with the MEK1 inhibitor blocked the EGF induced migration of these cells and selectively prevented EGF induced ERK1 and ERK2 phosphorylation without impacting other EGFdependent phosphorylation events, including the autophosphorylation of the EGF receptor (data not shown)."
9128257
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"When analyzed with the phospho-specific MAPK antibody, we observed similar profiles of time-courses and concentration-responses of EGF-induced phosphorylation of ERK2 and ERK1 as compared to those of [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12644307
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"As shown in Fig. 2 b, dose–response studies revealed that EGF-induced phosphorylation of ERK2 and ERK1 was maximal when the cells were treated with 1–10 ng/ml of EGF for 5 min."
12644307
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"Binding of NS5A non-structural protein of HCV to the growth factor receptor-binding protein 2 (Grb2) adaptor protein inhibits the EGF-induced phosphorylation of ERK1 and ERK2 in infected cells and disrupts the signal transduction pathways."
38526562
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"Time-course studies ( Fig. 3 b) revealed that EGF-induced phosphorylation of ERK2 and ERK1 was maximal at 2–30 min, declined at 60 min and returned to basal levels at 120 min after treatment with EGF [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12644307
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"Importantly, EGF and EST used alone or in combination with SSTR3 agonist induced ERK2 phosphorylation approximately 4-fold over the basal levels."
26112183
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"To test this, we first examined the effects of the specific MEK1 inhibitor PD098059 on phosphorylation of ERK2 and ERK1 by EGF in bovine luteal cells."
12644307
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"Western blot analysis with the active MAPK antibody showed that following pretreatment with 20 or 50 μM of PD098059 for 60 min, EGF-induced ERK2 and ERK1 phosphorylation was completely attenuated ( Fi[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12644307
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"As shown ( xref ), we found 12 phosphatases that had a significant effect on PDBu and/or EGF-stimulated ERK2 phosphorylation and/or compartmentalization ( xref )."
18650424
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"Epidermal growth factor induces phosphorylation of extracellular signal-regu- lated kinase 2 via multiple pathways."
7834738
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"In contrast, EGF driven ERK1 and ERK2 phosphorylation in BEAS-2B AECs was unaffected by inhibition of TPL2 with C1 (XREF_FIG)."
23527104
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"EGF stimulates phosphorylation of mitogen-activated protein kinase 1 (ERK2), thereby altering its structure and making Cys65 solvent-exposed."
37248656
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"Here we report that expression of p21rasAsn-17 does not abolish epidermal growth factor (EGF)-induced phosphorylation of ERK2 in fibroblasts."
8246947
sparser
"TGF-beta isoform-specific signaling also significantly attenuated EGF-induced phosphorylation of the mitogen-activated protein (MAP) kinase extracellular signal-regulated kinase 2."
9277449
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"These results demonstrate that in fibroblasts, EGF induces alternative pathways of ERK2 phosphorylation in a cell-type-specific manner."
8246947
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"ERK2 phosphorylation and activation are generally induced by EGF through EGFR and the cytoplasmic RAS/RAF/MEK pathway, with transient responses observed within a period of less than 1 h [18] ."
24735981
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"Burgering, B. M. T ., de Vries-Smits, A. M. M., Medema, R. H ., van Weeren, P . C., T ertoolen, L. G. J. and Bos, J. L., 1993, Epidermal growth factor induces phosphorylation of extracellular signal-regulated kinase 2 via multiple pathways."
11258851
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"In one example, EGF-induced ERK2 phosphorylation of CK2α followed by CK2 phosphorylation of α-catenin, thus releasing β-catenin for transactivation to the nucleus [ xref ]."
36114992
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"Interestingly, EGF causes phosphorylation of ERK2 only in K- Ras G12D mutated PDECs ( xref )."
22160930
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"Mitogen-activated protein kinase (MAPK) analysis revealed that in Hep2 cells, but not in CO-K3 cells, EGF induced a time-dependent phosphorylation of p42, p44, p38, and p46."
14649542
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"Despite the absence of significant elevation of basal ERK1/2 phosphorylation in P-UAEC-adEGFR, treatment with EGF induced robust phosphorylations of ERK1 ( P = 0.001) and ERK2 ( P = 0.001) at several times the levels observed in control cells, thus confirming a functional coupling to the MAPK pathway."
31550517
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"Epidermal growth factor induces phosphorylation of extracellular signal regulated kinase 2 via multiple pathways."
7888635
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"On the contrary, neither the Erk1 and Erk2 phosphorylation induced by EGF, nor their phosphorylation stimulated by protein kinase C in phorbol ester treated cells were sensitive to the effect of LY294[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
10659996
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"Functional AT (2)-receptors together with Ang II were identified in both cell lines and stimulation of these receptors inhibited EGF induced DNA synthesis and ERK2 phosphorylation."
18288685
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"Other recent studies show that blockade of endocytosis of EGF receptors by expression of mutant dynamin causes inhibition of EGF dependent phosphorylation of ERK1, ERK2, and PI3K."
9182681