IndraLab

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"HEK293T cells were seeded in 24-well plates and transfected with various combinations of plasmids DNA: the pEFneo-RIG-I, pEFneo-MDA5, pN1-IPS-1, pEFneo-TBK1, pEFneo-IKKɛ, pcDNA3-TRIF, or pCAGGS-IRF3 was mixed with a plasmid encoding the PRRSV protein (or empty pCAGGS), luciferase reporter plasmid and pSV40-RL."

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"The pEGFP-STAT1 and pN1-IPS-1 plasmids were constructed by PCR amplification of STAT1 and IPS-1 genes from the full-length cDNA clones (ATCC IMAGE clone ID 3627218 and ID 5751684, respectively), and subsequently cloned into pEGFP-N1 vector."