
IndraLab
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"For instance, protein levels of p-P70S6K and p-S6 in the mTOR pathway were shown to be decreased in PRAS40 KO mice (XREF_FIG), but these two proteins were not examined in PRAS40 transfected brains, even though we have shown that PRAS40 vector transfection promoted mTOR activity (XREF_FIG)."
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"XREF_BIBR, XREF_BIBR Nevertheless, considerable evidence exists to show that insulin can enhance the activation of mTOR via stimulation of 4EBP1 binding to dimeric mTOR complex 1, XREF_BIBR and mediated by the Akt and PKB substrate PRAS40 (proline rich Akt and PKB substrate 40 kDa)."
"Furthermore, PRAS40 phosphorylation by Akt and association with 14-3-3, a cytosolic anchor protein, are crucial for insulin to stimulate mTOR. -- (From Results Section) Because insulin stimulates Akt-mediated phosphorylation of PRAS40 at Thr 246 (ref. 26), we considered the possibility that PRAS40 phosphorylation may influence insulin activation of mTOR. Both wild-type PRAS40 and mutant PRAS40T246A expressed in 293T cells inhibited S6K1 phosphorylation and this inhibition was reduced on Akt1 coexpression with wild-type PRAS40 but not PRAS40T246A--"