"Upon phosphorylation, PRAS40 dissociates from the mTOR complex and increases mTOR kinase activity."
"XREF_BIBR, XREF_BIBR Nevertheless, considerable evidence exists to show that insulin can enhance the activation of mTOR via stimulation of 4EBP1 binding to dimeric mTOR complex 1, XREF_BIBR and mediated by the Akt and PKB substrate PRAS40 (proline rich Akt and PKB substrate 40 kDa)."
"The induced phosphorylation of PRAS40 has been posited to increase mTOR activity [XREF_BIBR; XREF_BIBR]."
"Akt mediated phosphorylation of PRAS40 induces mTOR activation XREF_BIBR."
"Several substrates overlap with AKT and mTOR signaling, including PRAS40 [XREF_BIBR], TSC2 [XREF_BIBR], 4EBP1 [XREF_BIBR], and EIF4B [XREF_BIBR - XREF_BIBR]."
"In summary, our study identifies that phosphorylation of PRAS40 may lead to the activation of mTOR signaling pathway in CNI induced rapid progression of human renal cancer."
"Furthermore, PRAS40 phosphorylation by Akt and association with 14-3-3, a cytosolic anchor protein, are crucial for insulin to stimulate mTOR."
"Both kinases phosphorylate proline rich-AKT substrate 40 (PRAS40), which increases mTOR kinase activity on dissociation from mTORC1."
"Silencing of PRAS40 by small interfering RNA suppresses the mTOR pathway signaling in response to insulin treatment in HEK293 cells, adipocytes, liver cancer cells and ESFT cells [XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR]."
"In fact, the observed decreases in eIF4E and several other proteins necessary for mTOR signaling such as Raptor, PRAS40, cyclin D1, and stearoyl CoA desaturase (SCD) a key enzyme in fatty acid synthesis, suggest reduction in mTOR associated cell proliferation and growth [XREF_BIBR, XREF_BIBR]."