IndraLab

Statements

USP50 affects PYCARD
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USP50 deubiquitinates PYCARD. 7 / 7
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"USP50 deubiquitinates the ASC protein."
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"The deubiquitinating enzyme, ubiquitin-specific peptidase 50, regulates inflammasome activation by targeting the ASC adaptor protein"
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"To further confirm our finding that USP50 deubiquitinates the ASC protein, we performed in vitro deubiquitination assays."
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"In conclusion, we here demonstrate a novel regulatory mechanism regarding the ASC protein which is deubiquitinated by USP50 and provide experimental evidence emphasizing the importance of a regulatory mechanism mediated by ubiquitination and deubiquitination in inflammasome activation."
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"However, the Flag-USP50 (C53S) mutant, did not affect polyubiquitination of the ASC protein, indicating that the Cys residue at position 53 is critical for the catalytic activity of USP50 deubiquitination of the ASC protein."
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"Ubiquitination of Flag-ASC proteins was significantly decreased in the presence of Flag-USP50 protein, indicating that USP50 directly deubiquitinates the polyubiquitination of the ASC protein."
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"Wild-type USP50 and the Flag-USP50 (H322A) mutant significantly deubiquitinated the ASC protein."
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USP50 deubiquitinates PYCARD on lysine. 1 / 1
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"Lee et al.66 showed that the deubiquitinating enzyme, USP50, binds to and deubiquitinates lysine 63‐linked polyubiquitin chains of ASC and that this is required for NLRP3 inflammasome activation, since USP50‐deficient cells show impaired inflammasome activation."
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USP50 deubiquitinates PYCARD at position 63. 1 / 1
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"Lee et al.66 showed that the deubiquitinating enzyme, USP50, binds to and deubiquitinates lysine 63‐linked polyubiquitin chains of ASC and that this is required for NLRP3 inflammasome activation, since USP50‐deficient cells show impaired inflammasome activation."
31679183
USP50-H322A deubiquitinates PYCARD. 1 / 1
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"Wild-type USP50 and the Flag-USP50 (H322A) mutant significantly deubiquitinated the ASC protein."
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USP50 affects WEE1
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USP50 deubiquitinates WEE1. 1 / 1
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"USP50 regulates HSP90-dependent Wee1 stability preventing mitotic entry, acting as another G2/M checkpoint"
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