IndraLab

Statements

ZRANB1 binds HECTD1.
4 | 3 8
ZRANB1 binds HECTD1. 10 / 14
4 | 2 8
biogrid
No evidence text available
23277359
biogrid
No evidence text available
23277359
reach
"GST pull-down was also used to further validate the interaction between TRABID and HECTD1."
| PMC
sparser
"Together, our data identify TRABID-HECTD1 as the first DUB-E3 pair regulating K29-linked polyubiquitin chains."
| PMC
sparser
"Although we attempted to validate the interaction between endogenous TRABID and endogenous HECTD1, none of the four TRABID antibodies that we tried could detect endogenous TRABID (Data not shown)."
| PMC
sparser
"Thus, this study identifies HECTD1 as a mammalian E3 ligase that assembles branched K29/K48 chains and also establishes TRABID-HECTD1 as a DUB/E3 pair regulating K29 linkages."
| PMC
reach
"Although we attempted to validate the interaction between endogenous TRABID and endogenous HECTD1, none of the four TRABID antibodies that we tried could detect endogenous TRABID (Data not shown)."
| PMC
biogrid
No evidence text available
19615732
sparser
"Thus, this study identifies HECTD1 as a mammalian E3 ligase that assembles branched K29/K48 chains and also establishes TRABID-HECTD1 as a DUB/E3 pair regulating K29 linkages."
33853758
sparser
"Thus, this study identifies HECTD1 as a mammalian E3 ligase which assembles branched K29/K48 chains and also establishes TRABID-HECTD1 as a DUB/E3 pair regulating K29 linkages."
33380427
ZRANB1 binds HECTD1 and APC. 1 / 1
| 1
reach
"Additionally, it was shown that the Trabid, Hectd1, and APC complex interacts with proteins composing the striatin interacting phosphatase and kinase (STRIPAK) complex that regulates cortical actin cytoskeleton dynamics [XREF_BIBR]."
29299163
ZRANB1 ubiquitinates HECTD1.
| 1
ZRANB1 ubiquitinates HECTD1. 1 / 1
| 1
reach
"Having established that TRABID NZF 1 to 3 is required for binding HECTD1 in cells and that catalytic dead TRABID traps polyubiquitinated HECTD1, we then validated this data in the context of HECTD1 's newly identified ubiquitin ligase activity using a pull-down approach (XREF_FIG, C and D; Fig.S3)."
| PMC
ZRANB1 increases the amount of HECTD1.
| 1
ZRANB1 increases the amount of HECTD1. 1 / 1
| 1
reach
"Importantly, this decrease in HECTD1 protein levels could be rescued by re-expression of pEGFP-TRABID WT but not pEGFP-Empty vector alone, indicating that TRABID directly regulates HECTD1 levels (XREF_FIG D)."
| PMC