"The PIP3, a product of PI3K, binds to AKT and leads to the membrane recruitment of the AKT, and it also binds to phosphoinositide dependent kinase 1 (PDK1) via their pleckstrin homology (PH) domains, then PDK1 phosphorylates AKT in the kinase domain (Thr 308 in AKT1)."
"PDK1 with its PH domain binds to either PIP3 or PIP2 and is translocated to the plasma membrane."
"PIP3 binds and activates PDK1 and PDK2 (3-phosphoinositide dependent protein kinases 1 and 2)."
"Akt and PDK1 bind PIP3 and translocate to the membrane [XREF_BIBR, XREF_BIBR]."
"In the membrane, the PDK1 and PIP3 complex phosphorylates AKT on thr308 XREF_BIBR."
"PIP3 binds and localizes the 3-phosphoinositide-dependent protein kinase-1 (PDK1) to the cell membrane, along with PDK1 's targets, Akt and atypical protein kinase C (aPKC)."
"To further examine the regulation of Akt1 activation, we studied PDK1, an upstream regulatory kinase, whose PH domain binds to the PI 3-K product PIP3 with high affinity."
"PDK1 and PKB and Akt bind to PIP3 and are recruited to the membrane XREF_BIBR, XREF_BIBR resulting in the phosphorylation of Akt by PDK1 and activation of Akt which leads to phosphorylation of both S6K and 4EBP1 XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR - XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR."
"PIP3 is bound by AKT which facilities its activation by PDK1."
"Second, ceramide blocks insulin stimulated Akt translocation to the PIP3 and PDK1 complex at the plasma membrane [XREF_BIBR, XREF_BIBR]."