IndraLab

Statements

OTUB1 affects UBE2D3
8 | 3 3
OTUB1 binds UBE2D3.
8 | 1 3
UBE2D3 binds OTUB1. 10 / 10
8 | 2
biogrid
No evidence text available
26752685
biogrid
No evidence text available
24403071
biogrid
No evidence text available
24403071
sparser
"These E2 enzymes have EC 50 values in the single digit micromolar range and lower the K M of OTUB1 for its substrate to 6.6 – 14 μM. Since the concentration of UBE2D3 alone has been reported to be 1.7 μM in MEF cells xref and the effective concentration of UBE2D3 needed to stimulate the DUB activity of OTUB1 is 1.6 μM ( xref ), it is likely that OTUB1:UBE2D3 form a stimulated DUB complex in cells."
32049508
biogrid
No evidence text available
25416956
biogrid
No evidence text available
32296183
biogrid
No evidence text available
32049508
biogrid
No evidence text available
32842143
sparser
"For example, (a) Ubiquitin transfer blockade: In multiple myeloma, OTUB1 interacted with the E2 enzyme UBE2D3 to inhibit ubiquitination of the transcription factor c-Maf, stabilizing its expression and promoting tumor cell survival ( xref ). (b) Direct target protein binding: OTUB1 suppressed ubiquitination of proteins (e.g., HIF-1α and RACK1) through non-catalytic binding, thereby driving tumor progression ( xref , xref ). (c) Phosphorylation-dependent functional switching: Phosphorylation of the Tyr26 residue in OTUB1 enabled its interaction with the cell cycle regulator p27, modulating p27 stability and cell cycle progression ( xref )."
40469292
biogrid
No evidence text available
37024477
UBE2D3-E2S binds OTUB1. 1 / 1
| 1
reach
"To verify that the interactions observed in the crystal structure are also observed in solution, the binding of the E2s UbcH5c (closely related to UbcH5b and also targeted by OTUB1) and UBC13 to full-length OTUB1 was examined by NMR spectroscopy."
22325355
OTUB1 binds UBE2D3 and UBE2N. 1 / 1
| 1
sparser
"To verify that the interactions observed in the crystal structure are also observed in solution, the binding of the E2s UbcH5c (closely related to UbcH5b and also targeted by OTUB1) and UBC13 to full-length OTUB1 was examined by NMR spectroscopy."
22325355
OTUB1 ubiquitinates UBE2D3.
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OTUB1 leads to the ubiquitination of UBE2D3. 1 / 1
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reach
"OTUD1 inhibits innate antiviral immunity in response to RNA and DNA viruses ( Zhang et al., 2018 , 2020b , 2020c ), OTUD4 promotes antiviral response by deubiquitinating and stabilizing MAVS ( Liuyu e[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
35675783
OTUB1 inhibits UBE2D3.
| 1
OTUB1 inhibits UBE2D3. 1 / 1
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reach
"Interestingly, this non canonical mode of action by OTUB1 was found not to be unique to UBC13, as OTUB1 was also found to interact with and inhibit the E2 enzymes UBE2D2 and UBCH5B and UBE2D3 and UBCH[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
21130715
UBE2D3 affects OTUB1
8 | 2 3
UBE2D3 binds OTUB1.
8 | 1 3
UBE2D3 binds OTUB1. 10 / 10
8 | 2
biogrid
No evidence text available
26752685
biogrid
No evidence text available
24403071
biogrid
No evidence text available
24403071
sparser
"These E2 enzymes have EC 50 values in the single digit micromolar range and lower the K M of OTUB1 for its substrate to 6.6 – 14 μM. Since the concentration of UBE2D3 alone has been reported to be 1.7 μM in MEF cells xref and the effective concentration of UBE2D3 needed to stimulate the DUB activity of OTUB1 is 1.6 μM ( xref ), it is likely that OTUB1:UBE2D3 form a stimulated DUB complex in cells."
32049508
biogrid
No evidence text available
25416956
biogrid
No evidence text available
32296183
biogrid
No evidence text available
32049508
biogrid
No evidence text available
32842143
sparser
"For example, (a) Ubiquitin transfer blockade: In multiple myeloma, OTUB1 interacted with the E2 enzyme UBE2D3 to inhibit ubiquitination of the transcription factor c-Maf, stabilizing its expression and promoting tumor cell survival ( xref ). (b) Direct target protein binding: OTUB1 suppressed ubiquitination of proteins (e.g., HIF-1α and RACK1) through non-catalytic binding, thereby driving tumor progression ( xref , xref ). (c) Phosphorylation-dependent functional switching: Phosphorylation of the Tyr26 residue in OTUB1 enabled its interaction with the cell cycle regulator p27, modulating p27 stability and cell cycle progression ( xref )."
40469292
biogrid
No evidence text available
37024477
UBE2D3-E2S binds OTUB1. 1 / 1
| 1
reach
"To verify that the interactions observed in the crystal structure are also observed in solution, the binding of the E2s UbcH5c (closely related to UbcH5b and also targeted by OTUB1) and UBC13 to full-length OTUB1 was examined by NMR spectroscopy."
22325355
OTUB1 binds UBE2D3 and UBE2N. 1 / 1
| 1
sparser
"To verify that the interactions observed in the crystal structure are also observed in solution, the binding of the E2s UbcH5c (closely related to UbcH5b and also targeted by OTUB1) and UBC13 to full-length OTUB1 was examined by NMR spectroscopy."
22325355
UBE2D3 activates OTUB1.
| 1
UBE2D3 activates OTUB1. 1 / 1
| 1
reach
"Since the concentration of UBE2D3 alone has been reported to be 1.7 muM in MEF cells 19 and the effective concentration of UBE2D3 needed to stimulate the DUB activity of OTUB1 is 1.6 muM (XREF_FIG), it is likely that OTUB1 : UBE2D3 form a stimulated DUB complex in cells."
32049508
OTUB1 affects UBE2D1, UBE2D2, UBE2D3, UBE2E1, and UBE2N
| 1
sparser
"To see if differences in relative stimulation of DUB activity can be attributed to variations in binding affinities, we determined equilibrium dissociation constants (K d ) for OTUB1 binding to UBE2D1, UBE2D2, UBE2D3, UBE2N, and UBE2E1 using isothermal titration calorimetry (ITC)."
32049508