IndraLab

Statements

UBE2D2 affects OTUB1
8 | 15 4
UBE2D2 binds OTUB1.
8 | 9 4
UBE2D2 binds OTUB1. 10 / 22
8 | 9 4
reach
"Addition of FAT10 also led to an increased interaction between OTUB1 and its cognate E2 UbcH5B implying a function of FAT10 in the inhibition of polyubiquitylation."
30718280
sparser
"These results indicated that EN523 does not interfere with binding of UBE2D2 to OTUB1."
35210618
sparser
"Binding of UBE2D2 to OTUB1, however, lowers the K M for of OTUB1 K48 chains by more than an order of magnitude to within range of physiological substrate concentrations xref ."
32049508
reach
"Resolution was extended by refinement at 3.3 A resolution against the P2 1 X-ray data set to an R factor / R free of 22.1/28.8%, with a doubling of the contents of the asymmetric unit The fold architectures of UbcH5b, OTUB1 and Ub have been described previously and do not deviate significantly in the Ub ~ UbcH5b and OTUB1-Ub complex (see XREF_SUPPLEMENTARY for sequence alignments and secondary structure assignments)."
22325355
biogrid
No evidence text available
28514442
reach
"To assess if free Ub could influence binding of OTUB1 to UbcH5b ~ Ub, we employed a time resolved (TR)-FRET-based assay where UbcH5b ~ Ub was labelled with Tb 3+ cryptate and OTUB1 was labelled with Alexa 488."
22325355
reach
"A near-complete model of the Ub ~ UbcH5b and OTUB1-Ub complex was refined at 3.8 A resolution to an R factor / R free of 21.7/27.9% with good geometry statistics (XREF_TABLE)."
22325355
sparser
"OTUB1 residue Glu28 is sandwiched between UBCH5B residues Lys66 and Arg90 and OTUB1-Asp35 hydrogen bonds with UBCH5B-Lys63, while OTUB1-Leu24 forms hydrophobic interactions with UBCH5B-Leu 119 ( xref )."
23955022
reach
"Compared with a previously published Ub ~ UbcH5b and OTUB1-Ub complex structure, Ubv.B1.1 is rotated by 22degrees relative to the distal site Ub.wt (XREF_FIG)."
23287719
reach
"These results indicated that EN523 does not interfere with binding of UBE2D2 to OTUB1."
35210618
UBE2D2 activates OTUB1.
| 5
UBE2D2 activates OTUB1. 5 / 8
| 5
reach
"To confirm that the observed stimulation was not assay dependent, we used a gel based assay to verify that UBCH5B stimulates OTUB1 cleavage of native Lys48 linked diubiquitin (XREF_FIG and XREF_SUPPLEMENTARY)."
23955022
reach
"Thes results indicate that UBCH5B stimulates OTUB1 by increasing its affinity for the Lys48 diubiquitin substrate."
23955022
reach
"We assayed the ability of UBCH5B ~ Ub to stimulate OTUB1 (XREF_FIG) and found that both charged and uncharged UBCH5B stimulate OTUB1 to a similar degree."
23955022
reach
"The importance of the OTUB1 N-terminal helix to its ability to be stimulated by UBCH5B, together with the role of these N-terminal residues in forming the proximal ubiquitin binding site of OTUB1 XREF_BIBR, XREF_BIBR, XREF_BIBR, suggested that UBCH5B may increase OTUB1 affinity for Lys48 diubiquitin via the proximal ubiquitin binding site."
23955022
reach
"However, assays of OTUB1 stimulation by UBCH5B or UBCH5B ~ Ub over a range of ubiquitin concentrations (XREF_FIG) (XREF_SUPPLEMENTARY) showed that stimulation by UBCH5B is relatively insensitive to free ubiquitin, but that stimulation by UBCH5B ~ Ub decreases as a function of increasing concentration of free ubiquitin, dropping to low levels below ~ 1 microM free ubiquitin."
23955022
UBE2D2 inhibits OTUB1.
| 1
UBE2D2 inhibits OTUB1. 1 / 1
| 1
reach
"A previous study 37 reported that the binding of UBE2D2 lowered the K M of OTUB1 for K48 diubiquitin by 34-fold, from 120 muM to 3.4 muM, with no significant change in k cat."
32049508
OTUB1 affects UBE2D2
8 | 14 6
OTUB1 binds UBE2D2.
8 | 9 4
UBE2D2 binds OTUB1. 10 / 22
8 | 9 4
reach
"Addition of FAT10 also led to an increased interaction between OTUB1 and its cognate E2 UbcH5B implying a function of FAT10 in the inhibition of polyubiquitylation."
30718280
sparser
"These results indicated that EN523 does not interfere with binding of UBE2D2 to OTUB1."
35210618
sparser
"Binding of UBE2D2 to OTUB1, however, lowers the K M for of OTUB1 K48 chains by more than an order of magnitude to within range of physiological substrate concentrations xref ."
32049508
reach
"Resolution was extended by refinement at 3.3 A resolution against the P2 1 X-ray data set to an R factor / R free of 22.1/28.8%, with a doubling of the contents of the asymmetric unit The fold architectures of UbcH5b, OTUB1 and Ub have been described previously and do not deviate significantly in the Ub ~ UbcH5b and OTUB1-Ub complex (see XREF_SUPPLEMENTARY for sequence alignments and secondary structure assignments)."
22325355
biogrid
No evidence text available
28514442
reach
"To assess if free Ub could influence binding of OTUB1 to UbcH5b ~ Ub, we employed a time resolved (TR)-FRET-based assay where UbcH5b ~ Ub was labelled with Tb 3+ cryptate and OTUB1 was labelled with Alexa 488."
22325355
reach
"A near-complete model of the Ub ~ UbcH5b and OTUB1-Ub complex was refined at 3.8 A resolution to an R factor / R free of 21.7/27.9% with good geometry statistics (XREF_TABLE)."
22325355
sparser
"OTUB1 residue Glu28 is sandwiched between UBCH5B residues Lys66 and Arg90 and OTUB1-Asp35 hydrogen bonds with UBCH5B-Lys63, while OTUB1-Leu24 forms hydrophobic interactions with UBCH5B-Leu 119 ( xref )."
23955022
reach
"Compared with a previously published Ub ~ UbcH5b and OTUB1-Ub complex structure, Ubv.B1.1 is rotated by 22degrees relative to the distal site Ub.wt (XREF_FIG)."
23287719
reach
"These results indicated that EN523 does not interfere with binding of UBE2D2 to OTUB1."
35210618
OTUB1 inhibits UBE2D2.
| 5 2
OTUB1 inhibits UBE2D2. 7 / 9
| 5 2
reach
"The OTUB1 double E28A D35A substitution, which affects OTUB1 contacts with UBCH5B, greatly decreased the ability of UBCH5B to stimulate OTUB1 DUB activity without affecting OTUB1 activity in the absence of UBCH5B (XREF_FIG)."
23955022
reach
"Similar interactions could form between OTUB1 and UBE2D2 (UBCH5b) (XREF_FIG), but clashes due to an insertion and a non conserved lysine would arise with UBE2L3 (UBCH7), consistent with the observation that OTUB1 inhibits UBCH5b but not UBCH7 4."
22367539
reach
"Interestingly, this non canonical mode of action by OTUB1 was found not to be unique to UBC13, as OTUB1 was also found to interact with and inhibit the E2 enzymes UBE2D2 and UBCH5B and UBE2D3 and UBCH[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
21130715
reach
"Competition with E3 binding is likely to be particularly important for OTUB1 inhibition of UBCH5b, which, unlike UBC13, is strictly dependent upon an E3 ligase for activity."
22367539
reach
"Since OTUB1 also inhibits UBCH5b 4, which does not function with a UEV, we speculate that the OTUB1 N-terminus may also interfere with acceptor ubiquitin binding for other E2s."
22367539
sparser
"Competition with E3 binding is likely to be particularly important for OTUB1 inhibition of UBCH5b, which, unlike UBC13, is strictly dependent upon an E3 ligase for activity."
22367539
sparser
"Similar interactions could form between OTUB1 and UBE2D2 (UBCH5b) ( xref ), but clashes due to an insertion and a non-conserved lysine would arise with UBE2L3 (UBCH7), consistent with the observation that OTUB1 inhibits UBCH5b but not UBCH7 xref ."
22367539
OTUB1 affects UBE2D1, UBE2D2, UBE2D3, UBE2E1, and UBE2N
| 1
sparser
"To see if differences in relative stimulation of DUB activity can be attributed to variations in binding affinities, we determined equilibrium dissociation constants (K d ) for OTUB1 binding to UBE2D1, UBE2D2, UBE2D3, UBE2N, and UBE2E1 using isothermal titration calorimetry (ITC)."
32049508