IndraLab

Statements

CHUK phosphorylates CYLD.

7 | 5 1

CHUK phosphorylates CYLD on S418. 4 / 4

1 | 2 1

reach

"Previous work demonstrated that TNF-alpha induces IKKalpha- and IKKbeta mediated serine phosphorylation of CYLD (Ser 418) [49]."

20347313

➶

signor

"Thus, serine 418 is phosphorylated in vivo. Cyld phosphorylation may serve as a mechanism to inactivate its traf2 deubiquitination activity."

24614225

➶

reach

"While it has been reported that IKKalpha and IKKbeta can phosphorylate Ser418 of CYLD, we demonstrated that CYLD is phosphorylated much more efficiently by IKKepsilon than by either of the canonical IKKs."

19481526

➶

sparser

"While it has been reported that IKKα and IKKβ can phosphorylate Ser418 of CYLD ( xref ), we demonstrated that CYLD is phosphorylated much more efficiently by IKKε than by either of the canonical IKKs."

19481526

CHUK phosphorylates CYLD. 2 / 2

| 2

➶

reach

"Interestingly, IKKalpha (I kappa B kinase alpha) and IKKbeta (I kappa B kinase beta) are also able to phosphorylate CYLD invitro, although invivo they require additional assistance of IKKgamma."

21480003

➶

reach

"Previous work demonstrated that TNF-alpha induces IKKalpha- and IKKbeta mediated serine phosphorylation of CYLD (Ser 418) [49]."

20347313

CHUK phosphorylates CYLD on S432. 1 / 1

1 |

➶

signor

"The phosphorylation of cyld was completely abolished by the combined mutations of the entire serine cluster (m4, lane 5). Similar results were obtained with the ikk holoenzyme (fig. 4c, panel 1), recombinant ikk_ (panel 2), and recombinant ikk_cyld phosphorylation may serve as a mechanism to inactivate its traf2 deubiquitination activity."

24614225