IndraLab

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Modified TP53 increases the amount of CDKN1A. 68 / 68
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"Overexpression of p53 increased p21 protein level in all cell lines (XREF_FIG)."
23300534
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"As expected ectopic expression of p53 increased p21 level in these cells, and interestingly we could not observe any significant effect of p53 expression on p21 activation in APE1 downregulated cells (XREF_FIG)."
23874636
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"Upon tet treatment, p53 levels rapidly increased, resulting in increased transcription of the p53 target genes p21, PIG3, and PUMA."
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"These results suggest that p53 significantly contributes to the expression of p21 in hMSCs, but the similar levels of p53 protein expression are not sufficient to induce the same level of p21 expression in hESCs."
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"(C) Western blot analysis demonstrated that the dominant negative p53 expression reduced the p21cip1/waf1 expression induced by p17 (lanes 3, 4, 5, and 6)."
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"Note that cells showing apoptotic signs (arrows) did not show high p21 WAF1 expression and that high p53 expression does not necessarily lead to p21 WAF1 expression and vice versa (arrows with attache[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"A significant decrease in p53 expression was found to be associated with an increased level of Mdm2 and decreased expression of p21 in HCV infected PHHs."
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"The results showed that the overexpression of p53 may increase the p21 levels (XREF_FIG right panel)."
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"Our data suggested that combined treatment of the C4-2B cell line with RES and DTX indicates that the up-regulation of p53 expression stimulates the expression of p21 WAF1 and CIP1 and p27 KIP1 and suppresses the expression of cyclin D1 and CDK4 complex thus blocks the progression of cells from G 1 to S phase."
28212547
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"Following exposure to 3 MEDs it was found that : (i) the maximum number of apoptotic cells occurred at 48 h; (ii) p53 protein expression was upregulated from 4 to 72 h preceding peak p21waf1 and Cip1 protein expression (9-48 h) and peak Bax protein expression (33 h)."
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"P21 protein levels are normally low, however in response to DNA damage, p53 activates transcription of the gene encoding p21 and hence increases p21 protein levels."
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"In a normal keratinocyte, DNA damage induces p53 expression that in turn up-regulates p21 expression."
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"In the KHOSR2 cell line (Mt-p53), overexpression of p53 up-regulates the expression of pro apoptotic protein p21 and Bax, while in the U-2OSR2 cell line (Wt-p53), overexpression of p53 down-regulates IGF-1r expression significantly."
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"Only when sunitinib was applied together with cisplatin or docetaxel, we observed an increase in the p53 protein level : the cytostatics activated DNA damage response mechanisms that in turn induced the upregulation of p53 protein expression; p53 was then translocated to the nucleus and induced p21 protein expression."
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"Ectopic expression of wild-type p53 induced both p21 expression and apoptosis."
9584145
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"We observed that the concurrent re-expression of DIRAS3 and p53 significantly induced the expression of p21 WAF1 and CIP1 and Bax compared to the treatment of cells with DIRAS3 or p53 individually."
33038921
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"Moreover, overexpression of a dominant negative p53 was shown to partially block the elevation in p21 transcription associated with increased HBV replication during doxorubicin treatment (XREF_FIG)."
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"The results suggested that high levels of p53 accumulation were associated with the expression of wild-type p53, which was able to activate the transcription of the p21 gene."
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"In the quiescence period, the increase in p53 expression activates p21 expression, which inhibits Rb phosphorylation and arrests the cell cycle in G1."
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"Compounds 10a and (-)-10a increase p53 protein levels, activate p53 dependent MDM2 and p21 transcription in MDM2 amplified cells, and show improved selectivity for growth inhibition in wild type p53 cell lines over the parent compound."
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"Loss of p53 increased the growth rate of cells and suppressed p21 expression."
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"Indeed, low levels of p53 have been demonstrated to induce p21 expression and cause cell cycle arrest XREF_BIBR XREF_BIBR."
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"In the KHOSR2 cell line with intrinsic mutant p53, overexpression of p53 up-regulated the expression of pro apoptotic protein p21 and Bax, while in the U-2OSR2 cell line with intrinsic wild type p53, overexpression of p53 significantly down-regulated IGF-1r expression (XREF_FIG)."
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"Pu-erh tea ethyl acetate extract blocked the progression of the cell cycle in HepG2 cells in G1 phase via the induction of p53 expression, which in turn up-regulated p21 expression."
28428754
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"Furthermore, the increased protein level of p53 was directly correlated with its transcriptional activity, indicated by S-18 phosphorylation of p53 in the spleens (p21 was undetectable in spleens; XREF_FIG), and increased p21 protein level in pBabe-c-Myc infected MEFs (XREF_FIG)."
25823025
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"Our results suggested that 95% hyperoxia could temporarily up-regulate the gene expression of p53, which induced the transcription of p21 (WAF and CIP1) mRNA."
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"The RNAi effect on hTERT caused an immediate increase in p53 mRNA levels, which in turn induced the transcription of p21 [23,24], suggesting that some specific telomeric effects of hTERT include the r[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"Loss of CDKN1A expression in response to loss of p53 and p73 is crucial for the induction of increased microtubule growth rates and W-CIN."
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"c-Myc overexpression in LoVo cells resulted in significantly increased p53 levels, which could lead to increased p21 Waf1 and Cip1 levels."
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"Similar to C4-2B cells, RES enhanced the expression of p53, which induces the increased expression of p21 WAF1 and CIP1 and p27 KIP1 in combination with DTX (22muM +10 nM) compared to DTX (31nM) and RES (35muM) only, which further inhibit the CDK4 and cyclin D1 complex by down-regulation of the protein expression."
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"In Hep G2, saikosaponin d blocked the progression of cell cycle at G1 phase by inducing p53 expression and further up-regulating p21 and WAF1 expression."
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"The elevated levels of p53 further up-regulate expression of p21 and that of the pro apoptotic p53 target genes BAX and PUMA in breast cancer cells."
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"As shown in XREF_FIG, overexpression of p53 increased Gadd45a and p21 transcript levels; however, CRM1 overexpression with p53 significantly suppressed p21 and Gadd45a induction, which was mediated by p53."
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"Co-expression of p53 and Agnoprotein further stimulated transcription of the p21 promoter."
12165856
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"Ectopical expression of p53 in tumor cells alone could induce p21 expression, followed by p21 cleavage and apoptosis."
9668108
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"Furthermore, one of the first effect of p53 expression, in nearly all mammalian cell types, has been reported to directly stimulate p21 expression for blocking the cell-division cycle XREF_BIBR."
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"Increased expression of p53 upregulates expression of CDKi (cyclin dependent kinase inhibitor) p21, which initially arrests the cell cycle."
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"Elevated p53 levels induce transcription of the gene encoding p21, an inhibitor of cyclin-Cdk complexes necessary for entry into S phase (reviewed by Hunter, 1993)."
7954799
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"We used the p53 plasmid to find that the overexpression of p53 increases the p21 protein level in ACC."
27351203
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"Radiation damage to DNA leads to elevation of p53 protein expression, which in turn induces the expression of the downstream regulatory factor, p21, and halts the cell cycle through the cyclin dependent kinase inhibitor (CDKI) mechanism."
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"The results showed that overexpression of p53 increased chemosensitivity in these drug resistant cells by up-regulating the expression of pro apoptotic protein p21 and Bax in KHOSR2, and down-regulating IGF-1r expression in U-2OSR2, respectively."
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"The increased p53 expression subsequently induced the expression of its downstream gene p21."
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"Suppression of p53 expression by small interfering RNA in RT4 cells restored Cdc25C expression and down-regulated p21 and WAF1 expression, which allowed Cdc25C and CDK1 activation, which then led to a G (2)-M arrest and an enhanced growth-inhibitory effect by flavokawain A. Consistently, flavokawain A also caused a pronounced CDK1 activation and G (2)-M arrest in p53 knockout but not in p53 wild-type HCT116 cells."
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"Over-expression of p53 markedly increased p21 levels in these cells."
26459178
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"Increased levels of p53 will, in turn, upregulate expression of the kinase inhibitor p21."
26437438
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"An increase in p53 expression induces the transcription of p21, thus initiating a growth arrest cascade."
12890637
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"Similar to C4-2B cells, RES enhanced the expression of p53, which induces the increased expression of p21 WAF1 and CIP1 and p27 KIP1 in combination with DTX (22muM +10 nM) compared to DTX (31nM) and RES (35muM) only, which further inhibit the CDK4 and cyclin D1 complex by down-regulation of the protein expression."
28212547
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"It is thus likely that APE1 overexpression and inactivation of p53, often observed in tumor cells, promote tumor cell proliferation by constitutively downregulating p21 expression."
23874636
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"We observed that the concurrent re-expression of DIRAS3 and p53 significantly induced the expression of p21 WAF1 and CIP1 and Bax compared to the treatment of cells with DIRAS3 or p53 individually."
33038921
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"In the present study, p21 expression may have been induced by p53 expression after DNA damage."
25754651
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"In A549, tetrazolium violet blocked the progression of the cell cycle at G1 phase by inducing p53 expression and further up-regulating p21 and WAF1 expression."
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"Elevations in p53 expression and p53 Ser15 phosphorylation enhance p53 transcriptional activity, resulting in increased MKP-1 and p21 levels, and increased gene expression of Wip1, p21, and Cdkn2d (Cdk4 inhibitor)."
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"Sakaida et al. observed that inhibiting endogenous NF-kappaB activity in Ras transformed NIH3T3 by over-expression of an IkappaBalphaSR or a dominant negative mutant of IKKbeta resulted in enhanced p21 expression, which may have been caused by elevated levels of p53."
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"Our experimental data presented here indicate that loss of TP53 and TP73 can trigger W-CIN by mediating a loss of CDKN1A."
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"The increased expression of p53, which is a DNA binding protein and functions as a tumor suppressor, can induce the expression of p21."
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"However, suppression of p53 expression in MDA MB 231 cells did not decrease p21 protein levels and did not reverse the effect of coculture on several cancer cell genes downregulation (XREF_FIG)."
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"The protein levels of Bax and p21, which are target molecules of p53, also increased under the same conditions, indicating that the increased p53 level was functionally linked to induce Bax and p21 expression in tCdc6 expressing HeLa cells (XREF_FIG)."
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"As shown in XREF_FIG, expression of p53 significantly increased the basal and PALA induced level of p21."
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"Overexpression of p53 by Ad-p53 infection induced p21 expression but no statistically significant difference was observed under normoxia (XREF_FIG)."
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"Expression of p21 and GADD45alpha after DNA damage typically is mediated by increased p53 levels, although p53 independent induction also occurs (Kaufmann and Paules, 1996; Kearsey et al., 1995; O'Rei[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15036396
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"As expected, expression of wildtype mouse p53 in p53-null human H1299 cells induced transcription of both p21 and PUMA (lane 2, XREF_FIG)."
22682249
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"Our data suggested that combined treatment of the C4-2B cell line with RES and DTX indicates that the up-regulation of p53 expression stimulates the expression of p21 WAF1 and CIP1 and p27 KIP1 and suppresses the expression of cyclin D1 and CDK4 complex thus blocks the progression of cells from G 1 to S phase."
28212547
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"Suppression of p53 expression by small interfering RNA in RT4 cells restored Cdc25C expression and down-regulated p21 and WAF1 expression, which allowed Cdc25C and CDK1 activation, which then led to a G (2)-M arrest and an enhanced growth-inhibitory effect by flavokawain A [XREF_BIBR]."
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"Here, we observed that in response to DOX, p21 level was induced by over-expression of p53 or p53 and p65."
22305266
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"Consistent with these findings, serine starvation increased p53 expression levels, which markedly induced p21 levels to trigger a transient cell cycle arrest, not observed in either p53 -\ - or p21 -\ - isogenic cancer cells."
23395165
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"Ectopic expression of wild-type p53 in WEHI 231 cells induced both p21 expression and apoptosis."
9584145
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"Our time-course of irradiation shows that both wtp53 and mutp53 accumulated quickly post irradiation; however, the high levels of p53 in both p53 +/- and p53 R172H/- testicular cells after irradiation did not induce p21 expression."
27869164
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"Importantly, in subconfluent 143B cells which are p53 mutant [XREF_BIBR, XREF_BIBR] induction of p21 transcription by ectopic expression of wild-type p53 did not upregulate FOXP2 and FOXP2 siRNA, demonstrating that this p21 induction by p53 was independent of the basal FOXP2 expression present in exponentially growing cells (XREF_FIG)."
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