IndraLab

Statements

USP30 activates PRKN. 10 / 10
| 10
reach
"Knockdown of the mitochondrial deubiquitinase, USP30, rescues mitophagy defects and disease in flies with pathogenic mutations in Parkin, suggesting a potential role for the inhibition of DUBs that target selective autophagy E3 ligases in the treatment of Parkinson 's and other diseases."
30633901
reach
"These results demonstrated that USP30 could promote d-gal-induced mitochondrial damage and cell senescence through antagonizing Parkin."
34290230
reach
"Although SILAC provides a rigorous way for performing quantification, other studies used label-free quantification to examine targeted mitochondrial outer membrane ubiquitylation by PARKIN in the presence or absence of the mitochondrial deubiquitylating enzyme USP30, leading to the identification of a dozen mitochondrial PARKIN targets that are regulated by USP30 (see below)."
26000850
reach
"Together, these data highlight the specificity of ntc and USP30 as important and opposing regulators of basal mitophagy.Given the previously established links between FBXO7 and USP30 with toxin-induced PINK1/Parkin mitophagy in cultured human cells, we next analysed whether the induction of basal mitophagy by ntc overexpression or USP30 knockdown involved the Pink1/parkin pathway in vivo."
37535686
reach
"Knockdown of USP30 rescues the defective mitophagy caused by pathogenic mutations in parkin and improves mitochondrial integrity in parkin- or PINK1 deficient flies."
24896179
reach
"More specifically, USP30 has been reported to mediate Parkin and PINK1-dependent mitophagy following the acute depolarization of the mitochondria [9] via the deubiquitylating Parkin substrates in the mitochondria [10]."
34290230
reach
"Knockdown of the Drosophila homologs of USP15 (CG8334, hereafter called dUSP15) and USP30 (CG3016, hereafter dUSP30) largely rescues the mitochondrial defects of parkin deficient fly muscle in vivo."
29809156
reach
"USP30 knockdown also increases the ubiquitination level on multiple Parkin substrates, thus confirming that USP30 antagonizes Parkin function."
30126257
reach
"Nevertheless, deletion of USP30 from cells with endogenous Parkin only results in a modest effect on mitophagic flux, and Parkin can rapidly overcome USP30’s activity to allow maximal activation of the Parkin dependent mitophagy ."
33890401
reach
"Silencing USP30 alleviates d-gal-induced mitochondrial damage and consequently suppresses myocardial cell senescence by activating Parkin."
34290230