IndraLab

Statements

TP53 binds GNL3 and MDM2. 7 / 7
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"By contrast, the NS-MDM2 and MDM2-p53 interactions were unaffected by the coexpression of p53 or NS, respectively ( xref and xref )."
19033382
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"Although it was previously reported that NS could directly interact with p53 xref , the functional meaning of this interaction had been obscure until the binding of NS to the p53 repressor, MDM2, was revealed later on xref ."
20703089
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"Co-immunoprecipitations revealed that I3C treatment of 10AT-Her2 cells strongly promoted nucleostemin binding to the MDM2 inhibitor of the p53 tumor suppressor, and thereby sequestered MDM2 into the nucleolus."
25209720
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"To assess whether the I3C regulation of MDM2 protein interactions with p53 and/or nucleostemin occurs in other indole-carbinol-sensitive breast cancer cells, three well-established cell lines, SKBR3, MCF-7 and MDA-MB-231, were treated with or without I3C for 48 hours and MDM2–p53 and nucleostemin–MDM2 co-immunoprecipitations carried out as described above for 10AT-Her2 cells."
25209720
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"Therefore, the effects of I3C on nucleostemin–MDM2 and MDM2–p53 interactions that we observed with 10AT-Her2 cells is not limited only to this newly developed breast cancer cell line."
25209720
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"The MDM2 interaction with p53 was partially reversed in I3C-treated cells after knockdown of nucleostemin, suggesting that in the absence of nucleostemin, MDM2 can still interact with p53, although at lower efficiency."
25209720
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"To define the interaction between NS, MDM2, and p53, HEK293 cells were triple-transfected with HA-tagged NS, FLAG-tagged MDM2, and/or Myc-tagged p53 expression plasmids, and immunoprecipitated with anti-tag antibodies."
19033382