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EGFR increases the amount of EGFR. 114 / 162
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"The increased expression of EGFR in HPNE cells that expressed K-ras (GD12) was mediated by both stabilizing EGFR protein and by increasing EGFR transcription."

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"EGFR inhibition by gefitinib further decreased confluence-induced suppression of DcR3 mRNA expression, and, vice versa, knocking down DcR3 expression attenuated EGFR and EGFR ligand expression as well as EGFR activation."

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"The STAT3 activating kinase, JAK2, is activated in GBM cell lines and combined inhibition of JAK2 and EGFR and EGFRvIII abolishes STAT3 activation and synergistically suppresses the growth of EGFR- and EGFRvIII expressing cell lines of breast cancer [XREF_BIBR] and epidermoid carcinoma [XREF_BIBR, XREF_BIBR], and GBM [XREF_BIBR]."

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"2,3 Bladder and kidney tumor volume was analyzed by the unpaired Student 's t -test.In the first set of studies, we determined whether the in vitro exposure of human dermal endothelial cells to EGF or[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"Through this interaction, CCN2 may be functioning as a regulator of EGFR signaling independent of EGFR or EGFR ligand expression."

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"Elevated levels of EGFR are usually induced by amplification of the EGFR gene and correlate with tumorigenicity and an unfavorable prognosis."

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"PKP2 induced phosphorylation of EGFR and subsequent ERK activation was entirely inhibited by the EGFR inhibitor lapatinib, which suggests that PKP2 exclusively mediates EGFR signal activation in EGFR expressing cancer cells."

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"The EGFR can induce cancer via at least three major mechanisms : overexpression of EGFR ligands, amplification of EGFR, and mutational activation of EGFR."

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"As shown in Figure 1 A, EGFR tyrosine kinase inhibitor, AEE788 (Traxler et al., 2004) (5.0 muM), did not decrease the expression of EGFR but did completely inhibit its phosphorylation."

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"We describe a clinically-applicable method to propagate and modify T cells to transiently express EGFR specific CAR to target EGFR expressing tumor cells that may be used to limit on-target, off-tissue toxicity to normal tissue."

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"In breast cancer, EGFR silencing decreased NRG1 expression and invasion, while NRG1 silencing decreased EGFR expression and proliferation [59]."

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"However, inhibition of her1 gene function in the zebrafish eliminates the oscillations in her1 and deltaC expression."

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"Interestingly, our results showed that KD of BCAP31 increased the recycling of EGFR to the membrane and the loss of resident EGFR in endocytic vesicles, decreasing overall membrane EGFR expression and autophosphorylation.Via observations of the interaction of BCAP31 with EGFR and its intracellular distribution, we found that although BCAP31 is mainly anchored to the ER, it co-localizes with EGFR throughout the whole endocardium system (Figure 5A-C)."

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"In tumor cells, the TK activity of EGFR may be dysregulated by various oncogenic mechanisms, including EGFR gene mutation, increased gene copy number and EGFR protein overexpression."

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"XREF_BIBR EGFR induces cancer in three ways : overexpression of EGFR ligands, amplification of EGFR, and mutational activation of EGFR."

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"TGF-alpha and HB-EGF (EGFR ligands) induce the expression of EGFRvIII, suggesting that EGFRvIII has a role in creating an autocrine loop with wild-type EGFR."

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"Our interpretation of this result is that these patients did not have EGFR driven tumors and hence no elevated level of EGFR on sEVs isolated from the MPE fluid."

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"In this case, HaCaT EGFR knockdown yields an anticipated and statistically significant decrease in EGFR expression (XREF_SUPPLEMENTARY; p-value of 9x10 -6)."

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"On the contrary, suppressing EGFR decreased the expression of p-EGFR, p-PI3K, and p-AKT (XREF_FIG)."

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"XREF_BIBR The biomarkers considered were EGFR gene copy number by FISH, EGFR protein expression by IHC, EGFR and K-RAS mutational status (XREF_FIG)."

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"Consistent with earlier reports (Ahsan et al., 2012), the inhibition of HSP90 by geldanamycin caused an approximately two-fold reduction in total EGFR levels, while loss of CHORDC1 in A431 KO cells reduced EGFR levels only by 20% (Figure 7G)."

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"For example, a Bispecific T-cell Engager (BiTE) targeted to the epidermal growth factor receptor (EGFR) produced severe liver and kidney toxicities in non-human primates, in line with EGFR expression in these organs, and led to the termination of the animals (Klinger et al., 2016; Lutterbuese et al., 2010)."

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"Silencing of EGFR reduced protein level of EGFR and that of SAE2."

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"In this study, miR-133b overexpression and EGFR down-regulation suppressed anoikis resistance and anchorage-independent growth in ESCC cells via inhibiting EGFR expression."

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"EGFR blockade significantly decreased expression of p-EGFR, inhibited cell cycle progression of astrocytes, and reduced reactive astrogliosis in vitro and in vivo."

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"Interestingly, we found that irreversible inhibitors of the EGFR signaling pathway increased in resistant cell lines the expression of multiple EGFR ligands."

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"In this model, all hypothetical patients have driver mutations that activate the EGFR or copy number variations that increase amounts of EGFR, either of which results in increased proliferation and tumor formation."

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"The results showed that EGF treatment trigger EGFR and Erk signaling by increased the levels of p-EGFR and p-Erk1/2."

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"Different deubiquitinating enzymes may be critical in balancing c-Cbl and SMURF2 activity [XREF_BIBR], thus tightly regulating EGFR protein stability, alteration of which during oncogenesis leads to EGFR overexpression."

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"Mechanistically, EGFR rich EVs mediated EGFR overexpression down-regulated intracellular ROS levels through the PI3K and AKT pathway, thus promoting the metastatic potential of poorly metastatic NPC cells."

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"Acquired mutations in the EGFR and ERK pathway can cause the overexpression of EGFR on the surface of the cell, while others downregulate the inactivation of switched on intracellular proteins such as Ras and Raf."

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"Interestedly, we also found induced PD-L1 expression in radiated ESCC cells with EGFR high expression, which was directly regulated by activated EGFR signaling resulting from radiation exposure."

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"Real-time RT-PCR showed that EGFR siRNA transfection caused a specific downregulation of EGFR mRNA expression by 21.05% in the tumor."

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"EGFR overexpression driven by EGFR gene amplification, though rare, has been reported in colon cancer."

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"Silence of EGFR expression by si-EGFR significantly inhibited the expression of EGFR."

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"The mechanisms of acquired or de novo resistance to EGFR targeting in EGFR expressing tumors are incompletely understood."

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"These results support the hypothesis that 3 ' EGFR poly A sequence alterations contribute to EGFR over-expression in MSI CRC."
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"The STAT3 activating kinase, JAK2, is activated in GBM cell lines and combined inhibition of JAK2 and EGFR and EGFRvIII abolishes STAT3 activation and synergistically suppresses the growth of EGFR- and EGFRvIII expressing cell lines of breast cancer [XREF_BIBR] and epidermoid carcinoma [XREF_BIBR, XREF_BIBR], and GBM [XREF_BIBR]."

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"The EGFR targeting shRNAs significantly inhibited EGFR expression, as verified by Western blotting."

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"These findings were consistent with a previous report showing that the EGFR targeting peptide GE11 mediated target specific delivery of reporter genes to EGFR overexpressing tumor cells in vitro."

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"The delay in EGFR endocytosis effectively increased the levels of endosomal EGFR, which triggered activation of ERK1/2 and cell proliferation via upregulation of cyclin D1."

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"MEHD7945A demonstrated nonlinear pharmacokinetics, with faster clearance at lower doses consistent with pharmacokinetic profiles of approved EGFR targeting monoclonal antibodies and wide expression of EGFR."

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"We now demonstrate that ARID3B directly activates EGFR, demonstrating a feed-forward pathway where EGFR signaling through ARID3B leads to increased EGFR expression."

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"Standardization of EGFR assay methods for clinical trials, as proposed by the EORTC Breast Cancer Cooperative Group [339], may allow more accurate assessment of the clinical utility of EGFR expression[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"In the latter case, this is achieved by transfecting the COS-7 cells with a plasmid encoding EGFR which increases the level of EGFR expressed within the cell."

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"We and others have reported that EGFR gene amplification contributes to EGFR overexpression in HNSCC."

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"Two different EGFR siRNAs independently knocked down EGFR expression and inhibited autophagic flux (XREF_FIG), and loss of EGFR markedly reduced endogenous LC3 puncta formation (XREF_FIG), indicating a role for EGFR in autophagy initiation."

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"Stimulation of the epidermal growth factor receptor (EGFR) in HaCaT cells enhances the level of EGFR associated PAK1 and Grb2, although the PAK1-Grb2 association is itself independent of this stimulation."

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"Our results further suggest that mutated EGFR protein may be the main contributor to the higher expression of EGFR in advanced-stage SCC or NSCLC, and this issue deserves further attention."

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"In conclusion, the Z EGFR :03115 -IR700DX showed specific uptake in vitro and enabled imaging of EGFR expression in the orthotopic brain tumor model."

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"EGFR silencing or FMN incubation decreased p-EGFR and p-p38 levels and down-regulated inflammatory cytokine levels in IL-1β-stimulated cells or supernatants."

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"The STAT3 activating kinase, JAK2, is activated in GBM cell lines and combined inhibition of JAK2 and EGFR and EGFRvIII abolishes STAT3 activation and synergistically suppresses the growth of EGFR- and EGFRvIII expressing cell lines of breast cancer [XREF_BIBR] and epidermoid carcinoma [XREF_BIBR, XREF_BIBR], and GBM [XREF_BIBR]."

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"To achieve this goal, targeting EGFR by RNA interference strategy has been sought to reduce EGFR expression and thus control its effects on tumor development."

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"In addition, NONO knockout markedly reversed the expression of nuclear EGFR targets which were induced by EGFR overexpression (Fig. 7E)."

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"44 The association between EGFR amplification, high Ki-67 labeling index, and low T1-Gad and PET (and also low T1-gad/T2) volume ratios is generally consistent with the notion that EGFR amplification leads to EGFR overexpression that promotes angiogenesis and aggressive tumor growth leading to glioma cell infiltration."

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"The STAT3 activating kinase, JAK2, is activated in GBM cell lines and combined inhibition of JAK2 and EGFR and EGFRvIII abolishes STAT3 activation and synergistically suppresses the growth of EGFR- and EGFRvIII expressing cell lines of breast cancer [XREF_BIBR] and epidermoid carcinoma [XREF_BIBR, XREF_BIBR], and GBM [XREF_BIBR]."

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"The BT112 cell line was derived from a glioblastoma with high-level EGFR amplification and expression of the mutant receptor variant EGFRvIII, which is maintained in vitro."

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"Moreover, it has been recently reported that treatment of EGFR overexpressing, ER negative breast cancer cells with SAHA could decrease the EGFR levels through destabilizing EGFR mRNA [XREF_BIBR], suggesting that combining anti-EGFR agents and HDACIs may provide a promising strategy for dual targeted therapy."

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"In ovarian cancer, estimates of the frequency of EGFR overexpression range from 10 to 70%, with EGFR expression reported on average in 48% of ovarian tumors."

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"In tumor cells, the tyrosine kinase activity of EGFR may be dysregulated by EGFR gene mutation, increased EGFR gene copy number, or EGFR protein overexpression, leading to aberrant EGFR signaling and increased tumor cell survival, proliferation, invasion and metastasis."

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"In OS, EGFR has been found to be overexpressed [17] and in vitro studies have reported the expression of EGFR as well as effective inhibition of OS growth by EGFR inhibitors [18, 19], but few activating mutations in the EGFR TK domain in OS cells [16, 19]."

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"It was reported that Pan-HER and EGFR targeting monoclonal antibody inhibited binding of EGFR to its receptor and disrupted EGFR dimerization levels which were evaluated by PLA [XREF_BIBR]."

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"HER1 mediated targeting was achieved in all HER1 expressing models."

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"This is consistent with previous findings showing that prolonged activation of EGFR enhances EGFR transcription through proteins downstream in the EGFR signaling pathway [XREF_BIBR, XREF_BIBR]."

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"To further determine whether ES expressed EGFR is activated we evaluated the expression of pEGFR in ES samples : 95% of EGFR expressing specimens exhibited positive staining at varying levels, whereas no EGFR phosphorylation was observed in only one sample (XREF_FIG)."

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"CA-SSR1 is a dinucleotide CA repeat of the EGFR gene that can modulate EGFR transcription and is a potential target of the mismatch repair machinery in tumours with microsatellite instability (MSI)."

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"However, transcriptome level analysis showed increased levels of EGFR mRNA in SNX3 silenced cells, suggesting activation of a compensatory mechanism to restore EGFR protein levels."

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"The EGFR induces cancer via at least three major mechanisms : overexpression of EGFR ligands, amplification of EGFR, and activating mutations of EGFR [8,9]."

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"Enhancing EGFR signaling through overexpression of the EGFR prevents DWMI, promotes the generation of new OLs and prevents behavioral deficits in different WM related tasks."

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"In this study, we analyzed whether cetuximab induced skin rash was correlated with distinct polymorphisms within the EGFR gene known to modulate EGFR expression, ligand binding, or signaling activity."

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"EGFR gene amplification and expression of EGFRvIII are both correlated with poor prognosis and shorter survival of GB patients ( xref )."

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"Overexpression of the EGFR is commonly caused by EGFR gene amplification and is sometimes associated with expression of a variant EGFR (de2-7 EGFR or EGFRvIII) bearing an internal deletion in its extracellular domain."

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"In addition, exploratory biomarker analyses, including the assessment of EGFR gene copy number by FISH, EGFR and p-AKT protein expression by IHC, EGFR, K-RAS and D-RAF mutational status, showed a trend towards a better survival outcome for gefitinib in patients with high EGFR-gene-copy number (HR 0.61 for high copy number and HR 1.16 for low copy number, P =.045), while patients with EGFR mutations obtained higher RR than wild-type patients (37.5% versus 2.6%) [XREF_BIBR]."

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"Pharmacological blockade of EGFR using erlotinib from Day 4 to Day 14 after induction of NTN was shown to reduce the expression of EGFR in the renal cortex, the proportion of crescentic glomeruli and blood urea nitrogen [XREF_BIBR]."

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"We have also shown that dual EGFR and HER2-TKI lapatinib enhanced the expression of EGFR or HER2, however, the EGFR-TKI gefitinib and pan-HER TKI afatinib did not enhance either receptor in MPM cells."

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"The present study has demonstrated that a LPEI-complexed EGFR-specific siRNA delivery system can efficiently and effectively inhibit EGFR expression in SPC-A1 cells in vitro."

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"As shown in Figure XREF_FIG, the combined PAFR and EGFR targeting decreased the phosphorylation levels of EGFR and beta-arrestin2 compared with AG1478 or WEB2086 treatment alone, without affecting the total amount of EGFR and PAFR."

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"Response to EGFR targeted therapy and EGFR expression do not correlate well in colorectal cancer (even in the context of KRAS mutation) and confirmed reports of significant disease response has been observed in colorectal cancer patients whose tumors express little to no EGFR as detected by immunohistochemistry."

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"We identified an EGFR-specific siRNA that reduced EGFR expression in A549 cells to 15% at the protein level compared to Neg."

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"EGFR activation promotes Sox2 expression, which in turn binds to the EGFR promoter and directly up-regulates EGFR expression."

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"Our developed HER1 gene driven luciferase reporter system and Western blot analysis of HER1 and NRF2 levels were used to evaluate and delineate the role of NRF2 in the regulation of HER1 receptor expression."

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"GKR and down-regulation of EGFR expression by antisense EGFR resulted in significant suppression of cell proliferation and induction of cell apoptosis in vitro."

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"Transduction of EGFR into D492 HER2 (D492 HER2 and EGFR) partially restored EGFR expression (XREF_FIG)."

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"In the present study, we report that the dual EGFR and HER2-TKI lapatinib may upregulate the expression of both EGFR and HER2, while neither the EGFR-TKI gefitinib nor the pan-HER-TKI afatinib demonstrate the same effect."

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"Normal hepatocytes express high levels of EGFR, EGFR activation contributes significantly to hepatocellular survival and proliferation during injury and regeneration, and also plays a fundamental role in the negative regulation of liver acute phase response triggered by inflammatory cytokines [XREF_BIBR]."
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"Combined PAFR and EGFR targeting synergistically diminished the expression of PAFR and EGFR phosphorylation and downstream signaling."

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"In addition, the effect of PDT on EGFR signaling in EGFR overexpressing A431 cells versus SK-ChA-1 cells is shown in more detail in Fig."

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"We demonstrated that Perp upregulation by cell detachment is driven by detachment induced loss of epidermal growth factor receptor (EGFR)."

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"In any event, our work showed that targeting EGFR levels and/or signaling network by restoring miR-124 augments the efficacy of EGFR and Her2 TKIs."

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"Taken together, most of the currently available datasets imply that radiosensitization of EGFR overexpressing tumour cells by EGFR specific antagonists affects EGFR signalling function in DNA-double s[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"Our study shows that 99m Tc-D10 represents a versatile tool for the specific detection of small EGFR overexpressing tumour lesions and the assessment of EGFR expression in tumours by in vivo SPECT."

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"We confirmed that siRNA knockdown of EGFR significantly reduced EGFR surface expression (XREF_FIG) and ADCC (XREF_FIG)."

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"Overexpression of the EGFR is commonly caused by EGFR gene amplification and is sometimes associated with expression of a variant EGFR (de2-7 EGFR or EGFRvIII) bearing an internal deletion in its extracellular domain."

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"These results suggest that the EGFR signaling pathway, including EGFR mRNA variant expression, could be involved in meningiomas oncogenesis."

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"The most frequent alteration is the amplification of the gene encoding the epidermal growth factor receptor (EGFR), causing a massive overexpression of EGFR."

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"Overexpression of the epidermal growth factor receptor (EGFR) is caused by EGFR gene amplification and is sometimes associated with expression of a variant EGFR (deletion exon 2-7 or EGFRvIII)."

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"These results suggested that EGFR-specific siRNA complexed with LPEI could efficiently reduce EGFR expression at both mRNA and protein levels in SPC-A1-xenografted tumors, with no non-specific RNAi effect being observed."

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"The absence of EGFR immunoreactivity in tumor cells does not support the use of EGFR expression as a prognostic indicator in patients with uveal melanoma."

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"23 Thus, it is possible that the EGFR SNP rs884419 may modulate expression of the EGFR gene."

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"IgA2 EGFR induces cytotoxicity of EGFR expressing Ba/F3 cells in a syngeneic intraperitoneal model."

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"Notably, the EGFR gene is located in the 7p11 region in the human genome, indicating that CCAT1 promotes EGFR transcription in trans."

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"In our previous report, the EGF signaling enhanced the transcriptional activity of Aurora-A gene through the nuclear EGFR signaling pathway in EGFR overexpressed A431 cell was demonstrated [XREF_BIBR]."

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"Lentivirus mediated EGFR SiRNA can inhibit the growth of HIPC in vivo and in vitro by effectively suppressing the expression of EGFR and its intracellular proteins."

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"The Egfr expression in the cells transfected with miR-sc8 inhibitor plus siRNA control was increased compared to that transfected with inhibitor control plus siRNA control, and the Egfr expression in the cells transfected with Egfr siRNA plus miR-sc8 inhibitor was similar to that transfected with inhibitor control plus siRNA control (XREF_FIG), suggesting that Egfr siRNA might attenuate miR-sc8 inhibitor induced increase in the Egfr expression to a certain degree."

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"Collectively these findings indicate that EGFR overexpression driven by EGFR 3 ' UTR mutations provides a growth advantage specifically in colon cancer cells devoid of constitutively activating downstream mutations."

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"Here we show that Egfr mediated signaling promotes Sox2 expression, which in turn binds to the Egfr promoter and directly upregulates Egfr expression."

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"Furthermore, the overexpression of LINC01225 promotes HCC proliferation by binding to EGFR, which subsequently increases the protein level of EGFR and activates a network of intracellular transduction of proliferative signals [XREF_BIBR]."

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"However, re-expression of EGFR rescued EGFR expression in EGFR-KD and BCAP31-KD cells, but neither EGFR Y845 nor Y1068 sites showed autophosphorylation or downstream activation (Figure 4G), indicating that the role of BCAP31 in EGFR signalling is kinase-independent and Y845-dependent."

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"Therefore, we speculated that the ligand independent activation of EGFR in cells expressing HER2 YVMA can also induce expression of multiple EGFR ligands."

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"Our results showed that Nimotuzumab treatment alone did not result in any changes of the total EGFR levels in both EGFR overexpressed KYSE30 and EGFR low expressed TE-1 ESCC cell lines."

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"As shown in XREF_FIG, EGFR siRNA significantly reduced miR-202-suppressed EGFR protein level in BIU-87 and EJ cells."

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"EGF induced EGFR activation and mTOR activation in EGFR expressing H460 and A549 NSCLC cell lines was inhibited."

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"The predominant green fluorescence demonstrates higher binding of EGFR antibody supporting a higher expression of EGFR in OVCAR-5 cells."

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"Crews’ group also reported a VHL-recruiting EGFR degrader 5 (PROTAC 4) derived from the second-generation covalent inhibitor afatinib, which effectively depleted the EGFR protein level in gefitinib-resistant cell lines harboring the EGFR double mutant."