IndraLab

Statements

BRAF-V600E phosphorylates MAP2K1. 4 / 5
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"In the RAF-MEK-ERK pathway, BRAF V600E results in constitutive phosphorylation of MEK1 (encoded by MAP2K1), which then activates ERK by phosphorylation, leading to malignant proliferation [4,6,98]."
| PMC
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"Immunoprecipitated BRAF (V600E) phosphorylated MEK1 protein in vitro (XREF_FIG, lane 5), however, treatment with 17-DMAG clearly inactivated the kinase activity of BRAF (V600E) (XREF_FIG, lane 6), even though equivalent levels of BRAF (V600E) protein were utilized from treated and non treated cell lysates (XREF_FIG, lanes 3 and 4)."
18676857
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"BRAF V600E increases 3-hydroxy-3-methylglutaryl-CoA lyase, intracellular levels of acetoacetate, and MEK1 phosphorylation, suggesting that there is crosstalk between metabolic pathways and cell signaling networks."
28828350
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"Although both BRAF V600E or BRAF WT phosphorylates MBP but such phosphorylation was not affected in the presence of increasing concentrations of acetoacetate or 3-HB (XREF_FIG and XREF_SUPPLEMENTARY), suggesting that acetoacetate may specifically affect BRAF V600E dependent phosphorylation of MEK1."
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