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TGFB1 increases the amount of SERPINE1. 147 / 189
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"In addition, in prostate cancer cells TGF-beta1 stimulates the expression and activity of uPA, PAI-1, and MMP-9, resulting in a net increment of pericellular plasminogen activation, increased activation of MMP-9, and finally increased tumour cell invasion and metastasis [XREF_BIBR, XREF_BIBR]."

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"TGF beta 1 enhanced the expression of PAI-1 but, unlike previous reports, epidermal growth factor did not have any notable effect on PAI-1 in these cells."

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"We therefore wanted to test whether TGF-beta1 could induce PAI-1 expression in the AT84 cells, and thus inhibit uPA activity and uPAR cleavage."

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"Moreover, TGF-beta1 increases PAI-1 plasma levels and promotes the epithelial-mesenchymal transition (EMT), while PAI-1 small interfering RNA prevents the TGF-beta1-induced EMT in mouse lung epithelial cells 12."

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"TGFbeta1 treatment (5 ng/mL, 12 h) in LX-2 cells increased the expression of TGFbeta1 and PAI-1, and those were also significantly inhibited by 0.3 and 1 mg/mL RGE pretreatment (XREF_FIG C, D)."

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"As determined by qRT-PCR and Western blot assay, TGF-beta1 increased the expression of SERPINE1 in a dose dependent manner (XREF_FIG and XREF_FIG)."

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"TGFbeta-1 can induce SERPINE1 expression but is inversely controlled by SERPINE1 via plasmin action [XREF_BIBR]."

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"The aim of this study was to identify signaling pathways through which TGF-beta1 upregulates PAI-1 expression in endothelial cells (EC) and test interventions for blocking these pathways."

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"Our current understanding of the pathways utilized by the TGF-beta1 to stimulate the PAI-1 transcription (summarized in XREF_FIG) indicate that this growth factor activates a kinase cascade, at least partially as a function of epidermal growth factor receptor mobilization (either through the release of the appropriate ligands or the direct receptor transactivation), involving MEK, ERK1/2, and perhaps p38 [XREF_BIBR - XREF_BIBR]."

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"As shown in Fig. 5 A, TGF-beta1 alone induced PAI-1 mRNA expression at 6 h."

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"TGF-beta1 activates two distinct type I receptor and smad signaling pathways with opposite effects in endothelial cells; the increased PAI-1 expression, and its anti-angiogenic consequences are in fav[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"Moreover, by using semiquantitative RT-PCR and Western blotting, we observed that only the expression of PAI-1 was modulated by TGF-beta1 treatment via a TGF-beta-inducible element contained in the PAI-1 promoter (CAGA box)."

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"Administration of 1D11 also suppressed expression of PAI-1 mRNA which is known to be induced by TGF-beta 1 (XREF_FIG)."

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"By contrast, depletion of coronin 1B by siRNA transfection increased TGFbeta1 induced Smad2/3 phosphorylation and PAI-1 expression in VSMCs."

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"In contrast, while TGF-beta 1 also increased expression of PAI-1 mRNA in NHBE cells, the expression of PA mRNA decreased simultaneously."

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"(1) TGF-beta1, in a concentration dependent manner, upregulated ALK5 and PAI-1 expressions in the HTFs, which peaked between 24 and 36 h."

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"Indeed recombinant GDF-15 and TGF-beta1 were both seen to induce the expression of plasminogen activator inhibitor 1, an established TGF-beta responsive gene, in RAW 264.7 macrophages (XREF_FIG)."

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"These same pathways are involved in TGF-β1-induced expression of the promigratory SERPIN PAI-1."

"It seems that the tumor growth and invasion need both the profibrinolytic and procoagulant activities triggered simultaneously in different regions of the tumor or in different cells. PAI-1 expression in tumor cells is stimulated by TGF-beta which is itself activated by uPA-generated plasmin."

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"28 TGF-beta1 stimulates plasminogen activator inhibitor 1 (PAI-1) expression in the kidney through the Smad-p53-Usf2 pathway in the UUO mouse model."

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"Importantly, TGF-beta1 in addition to upregulating MMP-10 has been shown to upregulate PAI-1 expression in HaCaT cells."

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"Aldosterone or TGF-beta1 alone may slightly increase PAI-1 expression in HSCs, which can be inhibited by spironolactone."

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"In this and recent studies, we show that, associated with depletion of lung GSH, TGF-beta1 increased PAI-1 expression and induced airway epithelial cell apoptosis, which was accompanied by massive fibrotic responses in mouse lung [XREF_BIBR] (XREF_FIG)."

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"As expected, TGF-beta1 significantly induced the expression of p15, PAI-1, and p21 both at mRNA and protein levels, whereas TGF-beta1 inhibited the expression of c-Myc."

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"Consistent with the activation of TGF-beta1 signaling in UUO, a dramatic increase of SMAD2/3 phosphorylation and PAI-1 protein expression occurs in the obstructed kidney compared with contralateral controls (XREF_FIG)."

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"In primary mesothelial cells, we found that TGFbeta1 significantly induced gene expression of alphaSMA, PAI-1 and type 1 collagen (XREF_FIG)."

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"The activation of TGFbeta1 induces the expression of PAI-1, a major inhibitor of fibrinolysis derived from endothelial cells, and modulates vascular barrier function via promoting matrix metalloprotease production 19."

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"TGF-beta1 may activate the plasmin system by stimulating gene expression of PAI-1, the principal inhibitor of plasminogen activation [XREF_BIBR]."

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"TAZ is required for maximal TGF-β1-mediated expression of the SMAD target gene encoding plasminogen activator inhibitor-1 (PAI-1), a potent profibrotic clade E member of the serine protease inhibitor family (SERPINE1) [ xref , xref , xref , xref ], and a similar involvement of YAP in TGF-β1-induced PAI-1 expression is evident in lung tumor cells [ xref ]."

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"TGF-beta1 treatment significantly increased the level of the PAI and decreased the level of tPA in a time- and dose dependent manner compared with the control group (XREF_FIG)."

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"As shown in XREF_FIG, TGF-beta1 stimulation significantly increased PAI-1 levels in the supernatants and PAI-1 mRNA expression in A549 cells."

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"atRA pretreatment effectively reduced fibronectin expression in glomerular mesangial cells stimulated with TGF-beta 1 or Ang II for 48 h. TGF-beta 1 stimulated PAI-1 expression reached a maximum at 5 h. atRA did n't affect the early (5 h) PAI-1 induction by TGF-beta 1, but markedly attenuated the sustained (48 h) PAI-1 induction."

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"Our current study demonstrated that AA significantly downregulated PAI-1 protein expression and blocked TGF-beta1 stimulated PAI-1 expression in keloid fibroblasts."

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"Previous reports identified that the PAI-1 expression is induced by TGF-beta1 via EGFR signaling pathway in human cancers XREF_BIBR."

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"The results showed that TGF-beta1 could induce tubular CTGF and PAI-1 mRNA expression."

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"In HeKa, TGFbeta1 exposure strongly induced mRNA expression of PAI1, MMP2, p15 INK4b, p57 Kip2, and SMAD7 and suppressed expression of c-MYC, an NF- kappaB target gene (XREF_FIG, XREF_SUPPLEMENTARY for UM-SCC-11B and 22A, and data not shown for p57 Kip2)."

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"Transforming growth factor-beta 1 (TGF-beta 1) rapidly increases the expression of junB transcription factor and plasminogen activator inhibitor-1 (PAI-1) and prevents the cell cycle dependent phosphorylation of the RB retinoblastoma susceptibility gene product during late G1 phase in Mv1Lu lung epithelial cells."

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"Identification of intracellular pathways through which TGF-beta1 upregulates PAI-1 expression in endothelial cells."

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"In such 3D cultures, TGF-beta1 increases expression of fibronectin, urokinase plasminogen activator (uPA), and PAI-1, and promotes tube formation [92]."

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"Also, it is important to note that TGF-beta 1 induced the expression and secretion of PAI-1 in Car C cells without affecting the net uPA secreted activity."

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"To assess the effect of TAK1-DN expression on TGF-beta signal transduction, we first examined the type-1 plasminogen activator inhibitor (PAI-1) expression induced by TGF-beta1 stimulation."

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"TAZ is required for maximal TGF-β1-mediated expression of the SMAD target gene encoding plasminogen activator inhibitor-1 (PAI-1), a potent profibrotic clade E member of the serine protease inhibitor family (SERPINE1) [27,28,29,30], and a similar involvement of YAP in TGF-β1-induced PAI-1 expression is evident in lung tumor cells [31]."

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"Consistent with these findings, p53 -/- fibroblasts are not inducible for increased PAI-1 expression in response to TGF-beta1 and pretreatment of Mv-1Lu mink lung cells (stably expressing a PAI-1 promoter-luciferase reporter construct) with the p53 inhibitor pifithrin-alpha effectively suppressed TGF-beta1-dependent PAI-1 transcription (XREF_FIG)."

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"A higher intensity of pleural inflammation in loculated effusions may enhance the release of TNF-alpha, IL-1beta, and TGF-beta1, which may subsequently increase the levels of PAI-1."

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"In our study, TGF-beta1 administration dose-dependently increased the expression of SERPINE1 in HT22 cells."

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"Individually, both EGF and TGF-beta1 enhanced PAI-1 mRNA expression (10- and 74-fold, respectively)."

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"In isolated TbetaRI- and TbetaRII expressing peritoneal macrophages, the activated TGF-beta1 induces the expression of the plasminogen activator inhibitor 1 (PAI-1), whereas in the mast cells, the levels of TbetaRI, TbetaRII, and PAI-1 expression were below detection."

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"Microphthalmia associated transcription factor (MITF), a member of the basic helix-loop-helix leucine zipper family of tissue specific transcription factors predominantly expressed in mast cells, melanocytes and osteoclasts, also stimulated PAI-1 gene transcription, and TGF-beta1 did not increase PAI-1 mRNA levels in mast cells from mi/mi mice expressing dominant negative MITF."

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"On the other hand, TIMP-1 and PAI-1 levels can also be modulated by TGFbeta1 XREF_BIBR, XREF_BIBR."

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"Studies have shown that TGFbeta1 induces plasminogen activator inhibitor 1 (PAI1) expression and demonstrated the requirement for EGFR in this process [XREF_BIBR - XREF_BIBR]."

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"XREF_BIBR, XREF_BIBR, XREF_BIBR In rat mesangial cells and renal fibroblasts, TGF-beta1 and aldosterone act synergistically to increase PAI-1 expression and decrease extracellular degradation."

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"Similar to MMP-10, PAI-1 expression in HaCaT II-4 cells is increased in response to TGF-beta1 stimulation, whereas the combination of TGF-beta1 + EGF synergistically enhanced PAI-1 protein levels (XREF_FIG; XREF_SUPPLEMENTARY)."

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"TGF-beta 1 also increases ECM protein levels and PAI-1 expression in NRK52E and HK-2 cells."

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"[XREF_BIBR] Likewise, TGFbeta1 levels were increased after TNFalpha administration, consistent with findings that TGF-beta1 induces PAI-1 expression."

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"However, the effects of CGRP on PAI-1 expression induced by TGFbeta1 are unknown."

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"RT-PCR showed that ASODN 1, 7, 8, 14, and 15 decreased PAI-1 mRNA expression induced by TGF-beta1 and AO14 showed the best inhibitory effect."

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"Transforming growth factor-beta1 (TGF-beta1) increased the expression of plasminogen activator inhibitor-1 (PAI-1), TGF-beta1 and collagen IV."

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"Adding to this, lately, experimental in vitro study revealed that PAI-1 transcription is initiated by transforming growth factor-beta1 (TGF-beta1) XREF_BIBR."

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"However, in primary cortical neurons, blocking of the serine protease HtrA1 (high temperature responsive antigen 1), which is known to regulate several pathologies, such as Alzheimer 's disease, macular degeneration, and osteoarthritis, led to overexpression of neuronal PAI-1 mediated by TGF-beta1 signaling and eventually to neuronal death."

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"TGFbeta1 induces PAI-1 expression in human esophageal epithelial cells and EoE fibroblasts."

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"In contrast, the TGF-beta1 concentration of 10 ng/mL further increased the expression of PAI1, a highly TGF-beta1 responsive gene (XREF_FIG)."

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"TGF-beta1 up-regulated beta-gal expression at the mRNA level, congruently with TGF-beta1 up-regulation of expression of the endogenous PAI-1 gene."

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"SKIP regulates the expression of uPA, PAI-1, and MMP-9 stimulated by TGF-beta1 in PC-3 cells."

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"XREF_BIBR Similarly, TGF-beta1 failed to stimulate PAI-1 expression in mouse embryonic fibroblasts (MEFs) genetically deficient in three src family kinases (i.e. c-src, c-yes-, c-fyn- null fibroblasts; SYF -/-/-) compared to identically stimulated wild-type SYF +/+/+ cells."

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"A DN- pp60 c- src construct completely blocked TGF-beta1-initiated PAI-1 induction while TGF-beta1 failed to stimulate PAI-1 expression in SYF -/-/- fibroblasts; importantly, PAI-1 expression was restored in SYF -/-/- cells engineered to re-express a wild-type pp60 c-src construct."

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"The PAI-1 mRNA expression induced by TGF-beta1 was significantly inhibited by CTGF antisense ODN."

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"Recently, TGF-beta1 directed expression of PAI-1 in astrocytes following injury has been shown to protect neurons from glutamate excitotoxicity [XREF_BIBR]."

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"Moreover, stable TRAIL-R1 knockdown in Colo357 cells increased TGFβ1-induced SERPINE1 expression and this effect was partially reversed by transient expression of the miR-370 mimic."

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"Active TGFbeta1 in the test sample binds to the receptors of the transfectants and induces PAI-1 expression, resulting in a dose dependent increase in luciferase activity in the cell lysate."

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"Western blotting showed that SB203580 inhibited pSmad2 and the expression of PAI-1 induced by TGF-beta1 as compared to the RKO cells (XREF_FIG)."

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"In the present study, TGF-beta1 was shown to increase mRNA and protein levels of PAI-1 and decrease those of uPA, while APE significantly suppressed TGF-beta1-induced PAI-1 expression and increased uPA expression in the presence of TGF-beta1 in a dose dependent manner, therefore reversing the effects of TGF-beta1."

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"We further demonstrate that TGF-beta1 increases PAI-1 expression in primary human esophageal epithelial cells and fibroblasts from patients with EoE."

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"We previously reported that SAS and HO-1-N1 were TGF-beta1-responsive hOSCC cells : TGF-beta1 upregulated expression of fibronectin and plasminogen activator inhibitor-1."

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"However, the expression of plasminogen activator inhibitor-1, which is known to be transcriptionally induced by TGF-beta1, was strongly induced by TGF-beta1 despite the marked downregulation of TbetaRII."

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"Exposure to high glucose alone induced a significant increase in fibronectin, PAI-1, and TGF-beta1, and PCS extract significantly reduced the mRNA expression of fibronectin and TGF-beta1, but increased the mRNA expression of PAI-1."

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"The Ang II induced PAI-1 mRNA expression was markedly augmented by TGF-beta1."

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"TGFbeta1 rapidly increased PAI-1 mRNA expression in the endothelial cells, and this effect lasted at least 24 hours."

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"Invitro assays revealed that TGF-beta1 stimulated the upregulation of Serpine1 mRNA expression, which was inhibited by TM5275 treatment in cultured HSC-T6 cells, a rat HSC cell line."

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"TGF-beta1 could not induce the expression of alpha-SMA, vimentin, fibronectin and PAI-1 or reduce the expression of E-cadherin in HK2 cells expressing Smad3 3S-A in response to TGF-beta1."

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"PAI-1 expression was upregulated approximately 2-fold after only 6 hours by all three doses of TGF-beta1 (p < 0.05 - XREF_FIG)."

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"Taken together, these results show that TGF-beta1 can increase PAI-1 expression in the AT84 cells and thus reduce cleavage of uPAR, most likely via inhibition and internalization of uPA."

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"Transforming growth factor beta1 (TGF-beta1)-treated HUVEC increased the expression of PAI-1 compared with the normal HUVEC."

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"XREF_FIG, TGF-beta1 significantly elevated PAI-1 expression in keloid fibroblasts, while both AA (30 muM) and Ros (2 muM) suppressed the PAI-1 upregulation induced by TGF-beta1 in keloid fibroblasts, which was abrogated by GW9662 (1 muM)."

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"In vitro assays revealed that TGF-beta1 stimulated the upregulation of Serpine1 mRNA expression, which was inhibited by TM5275 treatment in cultured HSC-T6 cells, a rat HSC cell line."
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"Blockade of several TGF-beta1 downstream pathways such as p38 MAPK or JNK, but not phosphatidylinositol 3-kinase/Akt and ERK1/2, only partially inhibited PAI-1 expression."

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"Aldosterone and TGF-beta1 synergistically increase PAI-1 expression in hepatic stellate cells of rats."

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"XREF_BIBR, XREF_BIBR, XREF_BIBR Overexpressed TGF-beta1 may increase the expression of PAI and TIMP-1, and simultaneously decrease the level of MMP-1 in the peritoneal mesothelial cell, which may result in decreased peritoneal fibrinolytic capacity and excessive deposition of ECM, and finally organizes to form dense adhesions."

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"We first examined PAI-1 expression, which is commonly known to be induced by TGF-beta1."

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"Since PAI-1 is a highly upregulated gene in the TGF-beta1 response set, TGF-beta1 may activate Akt through PAI-1 or, at least, induce PAI-1 expression through an Akt dependent pathway."

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"If TRAIL-R1 inhibits TGFβ1-dependent PAI-1 expression through miR-370-3p-mediated down-regulation of TGFβ-RII, then the enhancement of the TGFβ1 effect on PAI-1 expression following TRAIL-R1 depletion (see xref ) should be rescued by transfection of a miR-370-3p mimic."

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"Similarly, TGF-beta1 failed to stimulate PAI-1 expression in cultured mouse embryonic fibroblasts (MEFs) genetically deficient in three src family kinases (i.e., c-src, c-yes-, c-fyn- null fibroblasts; SYF -/-/-) compared to identically stimulated wild-type SYF +/+/+ cells."

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"TGF-beta1 greatly enhanced the production of uPA and MMP-9, as determined by zymography assays (XREF_FIG), as well as the expression of PAI-1 mRNA transcript determined by RT-PCR (XREF_FIG)."

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"Furthermore, TGF-beta1 has been shown to increase gene and protein expression of PAI-1 in cultured mesothelial cells, which enhanced fibrin deposition [XREF_BIBR]."

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"Furthermore, PAI-1 expression was upregulated by TGF-beta1 in PASMC, both at the mRNA and protein level, and controlled the proliferation, migration and adhesion of human primary PASMC."

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"It has been demonstrated that activation of TGF-beta 1 signaling triggers a dramatic induction of Smad2/3 phosphorylation and PAI-1 protein expression in the obstructive kidney XREF_BIBR."

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"To elucidate the mechanism of the transforming growth factor-beta1 (TGF-beta1)-dependent up-regulation of PAI-1 expression, we investigated which signaling pathway transduced by TGF-beta1 is responsible for this effect."

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"Irradiation or TGF-beta1 alone increased expression of alpha-SMA, collagen 1, CTGF, PAI-1 and fibronectin."

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"[Transforming growth factor beta 1 stimulates the expression of plasminogen activator inhibitor 1 in hepatic stellate cells]."

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"We previously demonstrated that TGF-beta1 induced the expression of pSmad2C, pSmad2L and pSmad3L, and increased PAI1 expression and the invasiveness of HepG2 cells."

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"In addition, p38 MAPK was reportedly involved in the regulation of PAI-1 protein expression mediated by the TGF-beta1 signaling pathway [XREF_BIBR] (XREF_FIG C)."

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"The upregulation of PAI-1 mRNA expression induced by TGFbeta1 in endothelial cells was inhibited by rhein in a dose dependent manner."

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"EMT markers, N-cadherin, PAI-1 and vimentin levels were upregulated by TGF-beta1 in MCF10A cells."

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"In contrast, canonical TGF-beta1 signaling leads to increased expression of PAI-1, and we have hypothesized that upregulation of PAI-1 through activation of the canonical Smad2/3 pathway may be involved a negative feedback of TGF-beta1 signaling."

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"The alpha-SMA, fibronectin and PAI-1 mRNA expression induced by TGF-beta1 were significantly inhibited by CTGF antisense oligodeoxynucleotide (ODN) transfection."

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"TGF-beta1 at 6 ng/mL induced the increased phosphorylation of Smad2/3 and the upregulated expression of alpha-SMA, COL1A2, PAI-1 and TIMP-1."

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"Our results on the control of PAI-1 gene expression by MITF isoforms, TGF-beta1 and activin A suggest that discrete regulation of the plasminogen activator system occurs in a cell type specific manner."

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"TGF-beta 1 modulation of urokinase and PAI-1 expression in human bronchial epithelial cells."

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"Despite similar distortion of acinar morphogenesis, only TGF-beta1, but not Col-1 stimulates the expression of the MYC, LOX, and PAI-1."

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"TGF-beta1 also, induces PAI-1 expression in human lung fibroblasts where nuclear MAPK phosphatase MKP-1 is a molecular target of Nox4 generated ROS leading to a sustained activation of JNK and p38 MAPKs [XREF_BIBR]."

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"These results collectively suggest that there is a non-Smad4-dependent pathway for TGF-beta-mediated signaling.In addition, our results that TGF-beta1 induces betaig-h3 expression but not PAI-1 expres[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"However, in the oral cancer cell line SasL1, TGFbeta1 failed to stimulate hBD-2 expression, while the expression of PAI-1, a TGFbeta target gene [42], was induced by TGFbeta1."

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"Similarly, silencing endoglin expression significantly reduced TGFbeta1 induced Type I Collagen mRNA and protein expression, PAI-1 expression (XREF_FIG), and connective tissue growth factor (CTGF) in hCF (XREF_SUPPLEMENTARY)."

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"Importantly, TGF-beta1 failed to induce PAI-1 expression in caveolin-1-null cells, correlating with reductions in both Rho-GTP loading and SMAD2/3 phosphorylation."

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"This study further shows that knockdown of ILK decreased uPA to PAI-1 ratio in ovarian cancer SKOV3 cells as evidenced by a decrease in basal and TGFbeta1 stimulated uPA protein level and an increase [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

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"Administration of 10 -6 mol/L Ang II or 5 ng/mL TGFbeta1 for 24 h significantly induced the mRNA expression of PAI-1, Col I and FN in cultured mouse MCs when compared with medium-alone control."

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"Endogenous PAI-1 gene expression induced by transforming growth factor-beta1 was significantly attenuated in embryonic fibroblasts derived from Tg mice compared with those from WT mice."

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"It is apparent, therefore, that TGF-beta1 stimulates PAI-1 expression through two distinct but cooperating pathways that involve EGFR and pp60c-src --> MEK and ERK signaling and EGFR independent, but Rho and ROCK modulated, TGF-betaR-directed SMAD and ERK activation [XREF_BIBR]."

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"Clearly, colchicine mediated PAI-1 or CTGF induction does not involve an autocrine TGF-beta1 loop regardless of commonalities in signaling elements (e.g., EGFR, Rho and ROCK, MEK-ERK1/2, SMAD2/3) that mediate TGF-beta1- and colchicine stimulated PAI-1 expression."

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"TGF-beta1 increased the level of PAI-1 by 12- to 25-fold in the presence and absence of plasminogen and tPA (p < 0.0001)."

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"Firstly, the PD model was established by TGF-beta1 stimulation in cultured HPMCs invitro, we found that TGF-beta1 significantly increased the EMT markers (alpha-SMA, vimentin, and fibronectin) and plasminogen activator inhibitor type 1 (PAI-1) expressions, but decreased epithelial marker (E-cadherin)."

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"TGFbeta1 may activate SP1, AP1 and Smad3-Smad4 and thus increase the expression of PAI-1 and collagen I."

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"TGF-beta1 treatment increased expression of SERPINE1 in all tested cells, while chondrosarcoma cell lines showed a significantly higher basal expression level, suggesting that the canonical TGF-beta pathway was more active in chondrosarcoma cell lines."

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"Different regulation of PAI-1 gene expression upon TGF-beta1 and activin A treatment was also detected in B16 melanocytes; TGF-beta1 transiently increased the PAI-1 mRNA level, whereas activin A had prolonged effects on up-regulation of PAI-1."

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"TSP-1 and TGF-beta1 promoted a dose dependent upregulation of ASPC-1 and COLO-357 PAI-1 expression."

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"TGF-beta1, by activating epidermal growth factor receptor (EGFR) signaling, stimulates the expression of the potent profibrotic matricellular plasminogen activator inhibitor-1 (PAI-1), which encodes the structural elements of ECM such as fibronectin and collagen 1 [XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR]."

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"The levels of tPA and PAI induced by 9 pmol/L TGF-beta1 for 4 hours were the lowest."

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"As shown in Fig. 1 A, TGF-beta1 increased PAI-1 mRNA level by 2.3-fold after 8 h treatment."

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"Because there are several consensus AP-1 binding sites in PAI-1 promoter [30], TGF-beta1 may induce PAI-1 expression at least in part through activating MAPK pathways."

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"PPAR-gamma antagonist abrogated the inhibition of AA and Ros on PAI-1 expression induced by TGF-beta1 in keloid fibroblasts."

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"The qRT-PCR results in both A549 and human primary SAECs suggested that TGFbeta1 modulated mRNA expression of SERPINE1 and FOXA2 in pulmonary epithelial cells through SMAD3."

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"Finally, TGF-beta 1 substantially increased the amount of intracellular and matrix associated PAI-1."

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"Although TGF-beta1 is known to increase PAI-1 expression, whether TGF-beta1 requires the ERK or the JNK pathways for induction of PAI-1 has not been examined yet in HMC."

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"HG and TGF-beta1 significantly increased PAI-1 and fibronectin protein expression as well as decreased plasmin and MMP-2 activity."

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"The demonstration of a close correlation between the PAI-1 and TGF-beta 1 mRNA levels and the severity of lupus like glomerular lesions suggests that a pertubation of the glomerular PA/PAI balance, resulting from a marked TGF-beta 1 mediated induction of PAI-1 gene expression, plays an important role in the progression of lupus like glomerular lesions, leading to glomerulosclerosis."

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"Taken together, this shows that TGF-beta1 is able to induce PAI-1 expression in both AT84-EV and AT84-uPAR regardless of the presence of FBS."

"TGFbeta1 evoked gradual accumulation of PAI-1 protein in LX2 cells, with peak PAI-1 levels observed from 6?24h after stimulation (Fig 2A-B). Increases in PAI-1 protein content were preceded by increased expression of PAI-1 mRNA, and levels of PAI-1 mRNA remained about 3-fold increased for 24h (Fig 2C)."

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"In the control experiment, the amount of PAI-1 in culture supernatants of blank vector introduced control Mv1Lu transformants (pCOS1) increased more than 10-fold by the addition of TGF-beta1."

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"Consistent with this hypothesis, Otsuka et al. observed less intimal hyperplasia in WT mice than in PAI-1-deficient mice, whereas up-regulation of arterial PAI-1 expression by TGF-beta-1 promoted intimal hyperplasia [XREF_BIBR]."

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"As a first step toward identifying whether PAI-1 could be an important regulator of adhesion formation during flexor tendon healing, we tested whether TGF-beta1 significantly upregulates the expression of PAI-1 gene and protein expression in flexor tendon tenocytes."

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"Aldosterone and TGF-beta1 may synergistically act on HSCs to increase PAI-1 expression as compared to treatment with aldosterone or TGF-beta1 alone."

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"EGF, phorbol esters or TGF-beta1 predominantly increased PAI-1 protein expression in TSH treated cells."

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"In view of the similarities in the repertoire of participating signaling intermediates (e.g., EGFR, c-src, Rho and Rock, MEK/ERK1/2) between the microtubule- and TGF-beta1-initiated pathways of PAI-1 induction, and previous observations that TGF-beta1 expression is also regulated by cytoskeletal deformation [XREF_BIBR], it was important to determine if colchicine stimulated PAI-1 expression was mediated by an autocrine TGF-beta1 loop."

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"TGF-beta1 can induce PAI-1 gene expression by phosphorylation of extracellular signal regulated kinases (ERK1/2) and p53."

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"As expected, TGF-beta1 significantly induced alpha-SMA, connective tissue growth factor, collagen 1alpha1, and plasminogen activator inhibitor 1 messenger RNA expression."

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"TGF-beta1 enhanced uPA production, the number of uPA cell surface binding sites, and the expression of the plasminogen activator inhibitor PAI-1, in transformed PDV cells, but had no major effect on nontumorigenic MCA3D keratinocytes."

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"In contrast, while TGF-beta1 also increased expression of PAI-1 mRNA in NHBE cells, expression of PA mRNA decreased simultaneously."

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"PL, TSP-1, PLs and TSP-1s all caused a drop in the PAI-1 protein expression by HMEC-1 at 24 and 48 h (Figs. 7 A, B), whereas TGF-beta1 and TGF-beta1s raised the PAI-1 protein expression."