IndraLab
Statements
TGFB1 activates epithelial to mesenchymal transition. 1000 / 2682
|
130
2546
6
reach
"Our findings establish an obligatory role for upstream translational control of downstream Snail1 mediated transcriptional events in TGF-beta1 induced EMT, and provide proof of concept for efforts to pharmacologically modulate the eIF4E-cap interaction as a means to inhibit pathological EMT in the setting of cancer and organ fibrosis."
reach
"First, our study demonstrated that TGF-beta1 made hepatoma cells became mesenchymal cells and adopted a fibroblast like morphology, and upregulated vimentin and fibronectin, downregulated E-cadherin significantly, which indicated that TGF-beta1 could induce hepatoma cells occurs EMT."
reach
"Previous EoE studies have shown that TGF-beta1 plays a role in esophageal remodeling, participates in esophageal smooth muscle contraction, stimulates fibroblast secretion of extracellular matrix, and contributes to esophageal epithelial to mesenchymal transition (EMT) XREF_BIBR, XREF_BIBR, XREF_BIBR - XREF_BIBR."
reach
"Li et al suggested the existence of the TGF-beta1/Smad/ILK/EMT axis, in which TGF-beta1 induces ILK to be expressed in tubular epithelial cells by means of Smad signaling, and if transduction pathways were restrained, ILK expression and the EMT process induced by TGF-beta1 were effectively prevented."
reach
"On the one hand, the induction of an EMT phenomenon that desensitizes delE746-A750-mutated PC-9 cells to erlotinib was found to proceed, at least in part, through the activation of IGF-1R -i.e., TGFbeta1 induced EMT is accompanied by IGF-1R activation at newly formed EMT related sub-cellular structures and the EMT process triggered by TGFbeta1 can not fully occur in the presence of IGF-1R inhibitors."
reach
"Cells were transfected with either control siRNA or Pak1 siRNA for 48 h then treated with either TGFbeta1 (5 ng/ml) or vehicle (sterile PBS) for additional 24 h. siRNA mediated Pak1 knock down blunted TGFbeta1 mediated increase in EMT markers N-Cadherin and Snail in PC3 cells (XREF_FIG)."
reach
"Transforming growth factor beta1 (TGFbeta1) is a key modulator of EMT in a variety of epithelial cells, but is also capable of inducing the myofibroblast phenotype, i.e. the acquisition of alpha smooth muscle actin (alphaSMA) microfilaments in fibroblasts during wound healing, in mesangial cells in culture and in renal tubular cells [XREF_BIBR]."
reach
"The concept that other factors (in this case, HCMV infection) might amplify the fibrogenic phenotype of HK-2 cells undergoing TGF-beta1 induced EMT has been validated by others, who have shown that epidermal growth factor enhances the migratory phenotype of HK-2 cells and synergistically increases MMP-9 production after TGF-beta1 induced EMT XREF_BIBR."
eidos
"The injured lung epithelium releases profibrotic growth factors and cytokines such as TGFbeta1 that promote epithelial-mesenchymal transition , recruitment , and activation of fibroblasts that cause aberrant lung tissue repair and excessive matrix deposition 33 CT fibrosis score * * * * * * * * WT / SAL / control IgG WT / SAL / anticorisin leads to denudation of the alveolar lining epithelium and replacement by the overgrowing fibroblasts ( Fig. 10 ) 32 ."
reach
"Snail1, which mediates TGFbeta1 induced EMT, was found to suppress the expression of the key lipogenic transcription regulators, carbohydrate response element binding protein (ChREBP) and sterol regulatory element binding protein (SREBP), in A549 lung adenocarcinoma cells, leading to downregulation of the fatty acid synthase, FASN [XREF_BIBR]."
| PMC
reach
"In agreement with previous reports, the present study demonstrated that TGFbeta-1 induces EMT in pancreatic cancer cells by acquisition of mesenchymal morphology, increased expression of the mesenchymal markers vimentin and N-cadherin, and decreased expression of the epithelial marker E-cadherin."
reach
"After optimization of the test with spiking experiments of A549 cells undergoing TGF- beta 1 induced EMT (A549 EMT), the CTCs EMT were enriched by immunomagnetic depletion of leukocytes and then characterized by a RT-PCR assay based on the retrieval of epithelial and EMT related genes."
reach
"Primary cholangiocytes isolated from our reporter strain were able to undergo EMT in vitro when treated with transforming growth factor-beta1 alone or in combination with tumor necrosis factor-alpha, as indicated by adoption of fibroblastoid morphology, intracellular relocalization of E-cadherin, and expression of alpha-smooth muscle actin (alpha-SMA)."
reach
"In agreement with previous reports [XREF_BIBR, XREF_BIBR], we showed that TGF-beta1 could induce EMT in MDA-MB-468 and MCF-7 cells, which included acquisition of mesenchymal phenotype, decreased expression of epithelial markers E-cadherin and increased expression of mesenchymal marker Vimentin and N-cadherin, as well as increased expression of CSC markers such as CD44."
reach
"TGF-beta1 treatment increased EMT in HK-2 cells in a time dependent manner, which resulted in the gradual increase in expression of alpha-SMA (XREF_FIG), an important marker of myofibroblast, and decrease in expression of E-cadherin, a typical phenotypic marker of epithelial cell (XREF_FIG)."
reach
"Since oxidative stress has been recognized as one of the contributors to this TGFbeta1 mediated pathology, we investigated the potential involvement of nuclear factor (erythroid derived 2)-like 2 (Nrf2), which is a key transcription factor for the regulation of multiple antioxidant genes, in TGFbeta1 stimulated EMT gene changes using the rat proximal tubular epithelial cell line NRK52E."
eidos
"However , TGFbeta1 induced EMT was unable to confer JQ1 resistance , with IC50 values for these cell lines remaining similar to those of H1975 parental cells ( 0.9 muM + / - 0.2 muM and 1.2 muM + / - 0.3 , respectively , Fig. 3C ) and far from the IC50 of H1975 JQ1 resistant cells ( > 10 muM , Fig. 3D ) ."
reach
"In line with these results, we observed that TGF-beta1 treatment significantly promoted the migration and invasion and EMT progress in bladder cancer cells, and ARMc8 silencing inhibited the TGF-beta1-induced migration and invasion, as well as suppressed the EMT progress in bladder cancer cells."
reach
"This conclusion is supported by studies showing that : 1) activation of EMT programming by TGF-beta1 increases LINE-1 mRNAs and protein; 2) the lung carcinogen benzo (a) pyrene coregulates TGF-beta1 and LINE-1 mRNAs, with LINE-1 positioned downstream of TGF-beta1 signaling; and, 3) forced expression of LINE-1 in BEAS-2B cells recapitulates EMT programming and induces malignant phenotypes and tumorigenesis in vivo."
reach
"The clinical relevance of EMT and drug insensitivity comes from recent studies showing an association between epithelial markers and sensitivity to erlotinib in NSCLC cell lines XREF_BIBR - XREF_BIBR, suggesting that EMT-type cells are resistant to erlotinib, however the role of signaling molecules in mediating the induction of EMT by TGF-beta1 is lacking."
reach
"Everolimus has been studied in the context of lung transplantation and has been of interest not only for its immunosuppressive, but also its antifibrotic and antitumor effects XREF_BIBR, XREF_BIBR Although the antitumor effects of everolimus, are mediated in part through effects on the PI3 and Akt TGFbeta1 signalling pathway XREF_BIBR, Smad phosphorylation XREF_BIBR and Snail expression XREF_BIBR, we found no effect of everolimus on TGFbeta1 induced EMT."
reach
"The results of early animal experiments indicated that febuxostat could alleviate kidney dysfunction in patients, and improve renal interstitial fibrosis in a rat model of UUO; however, whether febuxostat serves a role in preventing renal tubular EMT induced by TGF-beta1 remains unknown."
reach
"Indeed, in NaK-beta 1 siRNA cells, activated Erk1/2 levels were 3- and 4-fold higher compared to parental or scrambled oligo transfected LLC-PK1 cells, respectively (XREF_FIG), suggesting that NaK-beta 1 levels are at least in part involved in sustained activation of Erk1/2 in TGF-beta 1 mediated EMT."
reach
"Although TNF-alpha accentuates TGF-beta1 driven EMT in primary human bronchial epithelial cells (PBECs), we hypothesized that other acute pro inflammatory cytokines elevated in the airways of patients with OB may also accentuate EMT and contribute to dysregulated epithelial wound repair."
reach
"XREF_BIBR Another study in MAL-12 murine hepatocytes showed that transforming growth factor beta1, a known promoter of tumor aggression, induces epithelial to mesenchymal transition (EMT) through inhibition of ferritin heavy chain, increasing the labile iron pool and generating reactive oxygen species (ROS)."
reach
"TGF-beta1 mediates the EMT process via numerous intracellular signal transduction pathways, including the canonical Smad pathway, mitogen activated protein kinases (MAPK), PI3K and Akt and small GTPases that control the activity or expression of factors related to EMT [XREF_BIBR]."
reach
"Taken together, our study provided the first line of evidence that ursolic acid could attenuate the EMT process induced by TGF-beta1 in human NSCLC H1975 cells associated with the inhibition of the TGF-beta1-integrin signaling pathway in the absence of regulatory effects on cell morphology."
reach
"It was demonstrated that HDAC6 dependent deacetylation of alpha-Tub in a human lung type II epithelial cell line (A549) exposed to TGF-beta1 induced EMT via Smad signaling, and disparate responses to alpha-Ac-Tub were observed in primary cultures of human pulmonary arterial endothelial cells and fibroblasts stimulated with TGF-beta1 XREF_BIBR."
reach
"In both models of TGF-beta1 driven EMT, three TF reporters corresponding to TFs with well-known roles in physiologic EMT had increased activation (BRACHYURY-r, SRF-r, and HIF1-r, which had dynamic increases and decreases in activity as noted above), while apoptosis related E2F1-r had decreased activity."
reach
"TGF-beta1 induced a typical EMT in HK-2 cells, while it was blocked by Sal B and SB-431542, as evidenced by blocking morphologic transformation, restoring E-cadherin and CK-18 expression, inhibiting alpha-SMA expression and F-actin reorganization, and down-regulating MMP-2/9 activities in TGF-beta1 mediated HK-2 cells."
reach
"We demonstrate that MN treatment alone, or a combination of MN and TGF-beta1 induces reactive oxygen species (ROS), cell death, and EMT in HCEnCs during rosette formation, resulting in upregulation of EMT- and FECD associated markers such as Snail1, N-cadherin, ZEB1, and transforming growth factor-beta-induced (TGFbetaI), respectively."
reach
"In a word, this study found that TGF-beta1 can induce EMT in GC cells, and up-regulate miR-21 while down-regulate PTEN; and miR-21 expression inhibition can promote the phosophorylation of AKt and inhibit EMT through up-regulating target gene PTEN, which was further verified by an in vivo experiment using a xenograft model in nude mice."
reach
"In BC patient samples, the tumor invasiveness is driven by the TGFbeta1 signaling pathway that promotes the EMT through cancer associated fibroblasts (a major component of the cancer stroma); in this system, TGFbeta1 is overexpressed in the presence of an upregulated ZEB2-AS1 (together with ZEB2) [XREF_BIBR]."
reach
"Using quantitative reverse transcription-PCR (RT-PCR), immunofluorescence, immunohistochemistry, western blotting, as well as gain- and loss-of-function studies and functional assays, the role of SNAI1 and SNAI2 in TGFbeta1 induced EMT in ATII cells in vitro was assessed; and the expression of SNAI transcription factors was analysed in experimental and human IPF in vivo."
reach
"Given the known regulatory role of 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase-3 (PFKFB3) in glycolysis and association of glycolytic activity with malignant features such as invasion, we sought to investigate whether TGFbeta1 regulates PFKFB3 expression and if PFKFB3 is involved in the TGFbeta1 mediated increase in the invasive ability of the Panc1 cell cline-a well established model of TGFbeta1 initiated EMT."
eidos
"While increasing the density of RGD adhesion ligands at a constant stiffness prompted metastatic carcinoma cells to self-assemble into structures that resembled healthy acini ( 177 ) , increasing adhesion ligand density in the presence of TGFbeta1 stimulated epithelial-mesenchymal transition ."
reach
"In our group, we sought to examine the effects of Englerin A in preventing the migration and invasion of RCC cell lines as well as to investigate whether Englerin A may inhibit the molecular changes associated with EMT induced by transforming growth factor-beta1 (TGF-beta1) [XREF_BIBR]."
reach
"Likewise, a previous study of EMT induction by TGF-beta1 on rat AECs reported common EMT hallmarks (i.e., down-regulation of epithelial markers, a fibroblast like morphology, and an up-regulation of mesenchymal markers, including vimentin, type I collagen, and alpha-SMA) that are reminiscent of our observed EMT induction elicited by TGF-beta1 in selected cell lines (A549 and H1975)."
reach
"We have shown earlier that EGCG and curcumin inhibit EMT induced by TGF-beta1 in RPE cells through inhibiting Smad-3 phosphorylation, while there is a report of EGCG inhibiting acetylation of Smad-3 in renal tubular cells XREF_BIBR, indicating varying mechanisms of EMT based on the cell type."
reach
"Collectively, these results demonstrate that i] radiation induced EMT in RLE-6TN cells is mediated by ROS/MEK/ERK and ROS and TGF-beta 1 signaling pathways and ii] the inhibitory role of TSA in radiation induced EMT appears to be due, at least in part, to its action of blocking ROS and TGF-beta1 signaling."
eidos
"The balance between cell-ECM and cell-cell adhesion regulates the switch between EMT and apoptosis downstream of TGFbeta1 Since shILK cells show enhanced cell-cell adhesion and preferentially undergo apoptosis instead of EMT in response to TGFbeta1 , we hypothesized that experimentally decreasing cell-cell adhesion may permit TGFbeta1 to induce EMT in these cells ."
eidos
"Even though it is common knowledge that TGF-beta1 induces EMT by activating the Smad pathway , TGF-beta1 could activate other non-Smad-dependent signalling pathway as well , such as the Akt / mTOR pathway ( Zhu et al. 2017 ) , the ERK-dependent and PPARgamma-dependent pathways ( Li et al. 2013 ) , and the PI3K / Akt / ERK signalling pathway ( Zhang et al. 2019 ) ."
reach
"We recently observed that HPMC constitutively express BMP-7, that high glucose, glucose based PD solution, and TGF-beta1 downregulated BMP-7 expression in HPMC, and that overexpression of BMP-7 in HPMC prevented EMT induced by TGF-beta1, suggesting BMP-7 as a potential therapeutic strategy for preventing EMT of HPMC."
reach
"Even though it is common knowledge that TGF-beta1 induces EMT by activating the Smad pathway, TGF-beta1 could activate other non-Smad-dependent signalling pathway as well, such as the Akt and mTOR pathway, the ERK dependent and PPARgamma dependent pathways, and the PI3K/Akt/ERK signalling pathway."
reach
"Cell treatment with the PKA inhibitor, 5muM Myr-PKI for 48h, added to culture medium 30min before TGF-beta1 treatment or co-treatment with TGF-beta1 and cAMP analogues until the end of the experiment reverted the effect of both cAMP analogues on all analyzed EMT markers induced by TGF-beta1."
reach
"To investigate the inhibitory effect of VPA on EMT induced by TGF-beta1 or irradiation in TE9 cells, we examined the expression pattern of the epithelial marker E-cadherin and the mesenchymal marker vimentin with or without VPA treatment under TGF-beta1 or irradiation stimulation by immunocytochemistry and western blot analysis (XREF_FIG)."
reach
"As previously shown [XREF_BIBR], TGFbeta1 treatment induced EMT in both A2780s and A2780cp cells, resulting in the acquisition of a mesenchymal (spindle like) phenotype, associated with the suppression of the epithelial marker E-cadherin, and strong expression of the mesenchymal marker N-cadherin."
reach
"Our previous work showed that Snail transcription factor was activated in transforming growth factor beta1 (TGF-beta1) induced EMT in retinal pigment epithelial (RPE) cells and may contribute to the development of retinal fibrotic disease such as proliferative vitreoretinopathy (PVR)."