IndraLab

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USP15 inhibits IFNB1. 14 / 14
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"Depletion of endogenous USP15 reduced the extent of IFN-beta promoter activation stimulated by GST-RIG-I (2CARD) alone or together with TRIM25 (XREF_FIG)."
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"The result showed that USP15 caused the dose-dependent inhibition of IFN-β promoter activity (Fig. 2a)."
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"Cellular ubiquitin specific proteases, USP21, USP3 and USP15, a subfamily of deubiqutinase, remove K63 linked polyubiquitin chains from RIG-I and block it to induce IFN-beta."
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"We found both USP15-HA and USP15-Myc dose-dependently inhibited the SEV- and RIG-I-N-induced activation of IFN-β (Supplementary Fig. S5)."
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"HEK293T cells were transfected with USP15-specific siRNAs or control siRNAs for 24 h. Cells were further infected with SEV or mock infected for 16 h before immunoblots with the indicated antibodies were performed.Figure 2: (a) USP15 inhibited the SEV-induced activation of the IFN-β promoter."
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"(b) USP15 inhibited SEV-induced IFN-β mRNA and protein levels."
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"In conclusion, our results provide clear evidence that USP15 deconjugates the Lys63-linked polyubiquitin chains from RIG-I.Since the activation of RIG-I signaling requires Lys63-linked ubiquitination, then we investigated whether the overexpression of USP15, which removes the Lys63-linked polyubiquitin from RIG-I, will decreases the RIG-I-mediated activation of IFN-β promoter activity and the activation mediated by its constitutively active mutant, RIG-I-N."
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"(c) Effects of USP15 knockdown on SEV-induced activation of the IFN-β promoter, IFN-β mRNA and protein levels."
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"We found both USP15-HA and USP15-Myc dose-dependently inhibited the SEV- and RIG-I-N-induced activation of IFN-beta."
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"In addition, USP15 dose-dependently inhibited RIG-I-N-triggered IFN-beta-Luc, ISRE-Luc, NF-kappaB-Luc, and IRF3-Luc reporter activities."
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"In addition, USP15 dose-dependently inhibited RIG-I-N-triggered IFN-β–Luc, ISRE–Luc, NF-κB–Luc, and IRF3–Luc reporter activities (Supplementary Fig. S3)."
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"As observed in HEK293T cells, ectopic expression of USP15 inhibits SEV induced IFN-beta promoter activation, while knockdown of USP15 by siRNA results in upregulation of the activation of IFN-beta, NF-kappaB and IRF3 promoters in A549 cells, as well as the production of IFNB1 and ISGs."
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"The result showed that USP15 caused the dose dependent inhibition of IFN-beta promoter activity (XREF_FIG)."
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"As observed in HEK293T cells, ectopic expression of USP15 inhibits SEV-induced IFN-β promoter activation, while knockdown of USP15 by siRNA results in upregulation of the activation of IFN-β, NF-κB and IRF3 promoters in A549 cells, as well as the production of IFNB1 and ISGs (Supplementary Fig. S2)."
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