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EGF phosphorylates EGFR on tyrosine. 155 / 155
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"We found that EGF induced the sustained tyrosine phosphorylation of the EGF receptor and sustained the activation of mitogen activated protein kinase in the cultured cortical neurons."
8542070
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"The EGF induced increase in [3H]-thymidine incorporation was blocked by AG 1478 (an EGF receptor tyrosine kinase antagonist), genistein, and herbimycin A (tyrosine kinase inhibitors), suggesting a role in the activation and tyrosine phosphorylation of the EGF receptor."
16823772
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"Thus, both SLO and EGF specifically activate Tyr phosphorylation of EGFR in MDA-MB-231 cells, but in different patterns."
21787862
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"In the nontransformed intestinal cell line IEC-6, expression of EGFR, Src homology and collagen protein (SHC), phospholipase C gamma 1 (PLC gamma), and their tyrosine phosphorylation in response to EGF was assayed by immunoblot."
9207933
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"Immunoprecipitates of cell lysates using anti- 164 phosphotyrosine antibodies, followed by immuno- blotting with the same antibody demonstrated clearly that EGF causes phosphorylation of the EGF recep[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
8388736
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"The epidermal growth factor (EGF) stimulates rapid tyrosine phosphorylation of the EGF receptor (EGFR)."
19836242
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"Two types of modified GM3 strongly alter EGF dependent phosphorylation of the EGF receptor in opposite directions, i.e., de-N-acetyl-GM3 (amino-GM3; NeuNH2 alpha 2 ---- 3Gal beta 1 ---- 4Glc beta 1 ---- 1 Ceramide) strongly promotes tyrosine phosphorylation of the EGF receptor of A431 cells, while lyso-GM3 (NeuNAc alpha 2 ---- 3Gal beta 1 ---- 4 Glc beta ---- 1 Sphingosine) as well as GM3 inhibit tyrosine phosphorylation of the EGF receptor in the same cells under the same conditions."
3498483
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"In addition, down-regulation of AGK reduced EGF stimulated tyrosine phosphorylation of the EGFR."
15939762
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"It should also be noted that in GEC, EGF induces tyrosine phosphorylation of both EGF-R and Neu, a RTK that is related to EGF-R, but does not bind EGF directly."
10550326
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"It can intervene in EGF signaling, as it enhances Tyr phosphorylation of epidermal growth factor receptor (EGFR) in response to EGF.Pathway Effect EGF-EGFR-Ras-ERK Cell motility EGF-EGFR-Ras-PI3K-PKB Cell survivalEpigen (Epgn) 10 is a widely expressed transmembrane glycoprotein that undergoes cleavage to release a soluble EGF-like domain-containing fragment."
| PMC
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"In addition, EGF induced tyrosine phosphorylation of the EGF receptor (EGFR) and tyrosine and serine phosphorylation of extracellular signal regulated kinase (ERK) were also inhibited by infection with Ad-PKG II and treatment with the NO donor or precursor."
26171055
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"In addition, the EGF stimulation of tyrosine phosphorylation of the EGF receptor was not modified after C3 transferase pretreatment."
9990300
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"We showed that [D-Lys6]GnRH-II reduces EGF-induced auto-tyrosine-phosphorylation of EGF-receptors via activation of a PTP and that EGF-induced activation of mitogen-activated protein kinase was blocked in cells treated with [D-Lys6]GnRH-II."
17016655
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"At 0 EGF stimulated tyrosine phosphorylation of the EGF receptor (EGFR) and other proteins."
7919223
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"The epidermal growth factor (EGF) stimulates rapid tyrosine phosphorylation of the EGF receptor (EGFR)."
19836242
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"Tyrosine phosphorylation of the EGF receptor and Shc were rapidly increased by EGF."
7498649
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"Each mutation caused significant EGF independent tyrosine phosphorylation of EGFR, and allowed the receptor to promote Ba/F3 cell mitogenesis in the absence of EGF, arguing that these are oncogenic mutations."
16953218
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"Treatment of cells with ligand (EGF) induced tyrosine phosphorylation of the EGFR and caused decreased binding of ZPR1."
9298995
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"Stimulation of T47D cells with epidermal growth factor (EGF) results in the activation of the intrinsic tyrosine kinases of the receptor and the phosphorylation of multiple cellular proteins including the receptor, scaffold molecules such as c-Cbl, adapter molecules such as Shc, and the serine/threonine protein kinase Akt. "
11994282
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"Epidermal growth factor (EGF)-induced tyrosine phosphorylation of the EGF receptor was strongly attenuated by PDT but unimpaired by C2-ceramide."
25048553
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"All ErbB1 TKIs (Iressa, ZRBA4 and ZR2003) attenuated EGF induced ErbB1 tyrosine phosphorylation in U87MG as well as U87 and EGFRvIII cells (XREF_FIG)."
22523681
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"An EGF stimulated protein kinase phosphorylates endogenous membrane proteins as well as EGF receptor (EGF-R) at tyrosine residues (9,10)."
6322750
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"In vitro, ABX-EGF, formerly known as clone E7.6.3 [21], binds EGFr with an affinity of 5x10 -11 M, blocks the binding of both EGF and TGF-alpha to the receptor, and inhibits EGF activated EGFr tyrosin[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
11255078
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"This phosphorylation of EGF receptors results in decreased self phosphorylation of the EGF receptor at tyrosine residues both in vivo and in vitro and in decreased EGF stimulated tyrosine kinase activity in vivo."
6321473
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"EGF induced sustained tyrosine phosphorylation of the EGF receptor in PC12h-R cells, but not in the parent PC12h cells, which do not show neuronal differentiation in response to EGF."
8873086
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"The data show that EGF caused a rapid and strong increase in tyrosine phosphorylation of the EGF receptor, with a maximum 5-15 s after the beginning of the incubation followed by a decline."
9176240
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"Stimulation of calcium influx by thapsigargin in a calcium containing medium did not modify EGF stimulated EGF receptor tyrosine phosphorylation."
9990300
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"We show that EGF-induced EGFR autotransphosphorylation is reduced in PC3-AR cells compared to PC3 cells transfected only with the vector (PC3-Neo)."
15305378
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"Immunoprecipitates of cell lysates using anti- 164 phosphotyrosine antibodies, followed by immuno- blotting with the same antibody demonstrated clearly that EGF causes phosphorylation of the [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
8388736
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"In DU 145 cells, LHRH-A antagonizes the proliferative action of EGF, inhibits tyrosine phosphorylation of EGF receptor induced by EGF and significantly reduces the number of EGF binding sites, without altering the stimulation of c-fos expression induced by EGF."
9396187
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"EGF and TGF-alpha induced a rapid increase in tyrosine phosphorylation of the EGF receptor, in both fibroblasts and keratinocytes, but failed to induce tyrosine phosphorylation of PLC-gamma 1 or detectable phosphoinositide hydrolysis, as measured by two sensitive assays."
7690546
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"However, pretreatment of cells with 200 nmol/L PD169540, which specifically inhibits EGFR and related ErbB tyrosine kinase activities through covalent modification of a cysteine residue in the ATP bin[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16936259
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"Immunoprecipitation studies demonstrated a reduction of EGF-induced tyrosine phosphorylation of EGFR in PC3-AR cells."
15288768
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"These results suggest that EGFR phosphorylation at Tyr-1068 does not trigger the nuclear accumulation of EGFR and that EGF promotes phosphorylation of nuclear EGFR at their tyrosine residues to differ[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
19766894
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"Further, it was observed that PDGF mimicked the action of phorbol diesters to inhibit the EGF dependent tyrosine phosphorylation of the EGF receptor in [32P] phosphate labeled fibroblasts."
2987962
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"These results provide strong evidence that EGF induces tyrosine phosphorylation of EGFR and Jak2 via auto-phosphorylation of these kinases, and also demonstrate that AG-490 and AG-1478 were effective under our experimental conditions."
19341767
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"Induction of RPTP-kappa by TGF-beta significantly decreases basal and EGF stimulated EGFR tyrosine phosphorylation."
19864455
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"Effect of EGF concentration on EGF- induced EGF receptor tyrosine phosphorylation in intact cells."
8796482
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"EGF induces tyrosine phosphorylation of EGFR and ErbB2 in GH3 cells, and these effects can be blocked by gefitinib."
21917845
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"A mouse monoclonal anti-EGFR antibody (clone 225) binds to EGFR with affinity similar to EGF (1nM), blocks EGF induced activation of EGFR tyrosine phosphorylation, and induces internalization of EGFR without stimulating EGFR phosphorylation XREF_BIBR."
21687663
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"Immunoprecipitation studies demonstrated a reduction of EGF-induced tyrosine phosphorylation of EGFR in PC3-AR cells."
15288768
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"EGF (10 ng/ml) and UVB irradiation (40 mJ/cm 2) induced tyrosine phosphorylation of EGFR in intact human keratinocyte HaCaT cells."
16936259
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"These data demonstrate that intraluminal EGF stimulates rapid tyrosine phosphorylation of the EGF receptor in vivo and that c-neu is a major substrate of the EGF receptor in suckling jejunum."
8101698
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"Published and unpublished data indicate that the functional difference appears to be due to the fact that EGF stimulates greater EGFR tyrosine phosphorylation at EGFR Tyr1045 than does AREG, resulting in greater EGFR coupling to the ubiquitin ligase c-Cbl and greater EGFR ubiquitination and more rapid EGFR turnover."
21532939
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"Both apical and basolateral EGF activated tyrosine phosphorylation of EGF receptors (EGFR), beta-catenin, and cellular substrate as evident on confocal microscopy."
11208549
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"The high affinity ligand, EGF, triggered more robust tyrosine phosphorylation of Egfr compared to the low affinity ligand, AREG, as determined by immunoblotting of liver extracts; however, similar activation of downstream Erk and Akt was found with both ligands (XREF_SUPPLEMENTARY)."
28494873
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"These results suggest that EGFR phosphorylation at Tyr 1068 does not trigger the nuclear accumulation of EGFR and that EGF promotes phosphorylation of nuclear EGFR at their tyrosine residues to differ[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
19766894
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"However, in the presence of 1mM ATP, EGF induced phosphorylation of tyrosine residues of EGFR can be revitalized in lysates prepared from MNNG pretreated cells."
16488447
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"We show that EGF-induced EGFR autotransphosphorylation is reduced in PC3-AR cells compared to PC3 cells transfected only with the vector (PC3-Neo)."
15305378
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"Our studies of the mechanism underlying the functional differences between ErbB4 full and partial agonists have been inspired by the observation that the EGFR full agonist AR stimulates a pattern of EGFR tyrosine phosphorylation that is distinct from the pattern of EGFR tyrosine phosphorylation stimulated by the EGFR partial agonist EGF [XREF_BIBR]."
22216880
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"Efficiency and specificity of the neutralizing antibodies were demonstrated by the inhibition of EGF stimulated EGFR tyrosine phosphorylation."
16636149
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"XREF_FIG shows that CCK-8, CCK-8NS, gastrin-17 and EGF increased significantly EGFR tyrosine phosphorylation by 233, 149, 208 and 250%, respectively."
25761747
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"EGF caused much greater tyrosine phosphorylation of the EGF-R than Ang II and PMA."
11809859
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"As shown in Fig. 2 (lane 2), EGF caused a striking increase in the tyrosine phosphorylation of the pancreatic acinar EGF receptor which migrated as a broad band of M r 170 000-175 000."
9990300
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"At 0 EGF stimulated tyrosine phosphorylation of the EGF receptor (EGFR) and other proteins."
7919223
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"To further verify if polyQ-htt did not affect specific EGFR phosphorylation, we determined the pattern of EGFR tyrosine phosphorylation induced by EGF stimulation using a panel of site specific antibo[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
22974559
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"Stimulation of T47D cells with epidermal growth factor (EGF) results in the activation of the intrinsic tyrosine kinases of the receptor and the phosphorylation of multiple cellular proteins including the receptor, scaffold molecules such as c-Cbl, adapter molecules such as Shc, and the serine/threonine protein kinase Akt."
11994282
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"EGF treatment induces EGFR tyrosine phosphorylation and recruits c-Cbl as well as other signaling molecules binding to activated EGFR."
24735711
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"PD153035 is a potent (Ki = 6 pm) and specific inhibitor of the epidermal growth factor (EGF) receptor tyrosine kinase that suppresses tyrosine phosphorylation of the EGF receptor in A431 cells at nanomolar concentrations in cell culture."
8824347
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"As shown in Fig. 1, a time course of stimulation by these growth factors revealed that EGF strongly induced tyrosine phosphorylation of the EGF receptor, c-Jun protein expression and DNA synthesis."
18722344
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"NEU3 enhances cancer cell survival [XREF_BIBR, XREF_BIBR], cell migration and attachment [XREF_BIBR], whereby it stimulates Ras activation with a consequent influence on ERK1/2 and Akt and actually enhances the EGF stimulated tyrosine phosphorylation of EGFR [XREF_BIBR]."
25803810
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"Indeed, treatment of EGFR positive cancer cells with CL4 strongly inhibits both the EGF induced tyrosine phosphorylation of EGFR and ErbB2 and the Hrg dependent tyrosine phosphorylation of EGFR and ErbB3."
21915281
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"We used mass spectrometry-based proteomics to comprehensively compare proteins that were tyrosine phosphorylated in response to EGF and PDGF and their associated partners. More than 90% of these signaling proteins were used by both ligands, whereas the phosphatidylinositol 3-kinase (PI3K) pathway was exclusively activated by PDGF,"
15933201
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"In smooth muscle cells, activation and clustering of integrin beta 3 promoted the EGF dependent tyrosine phosphorylation of EGFR [XREF_BIBR]."
19267251
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"Ability of EGF, TGFalpha or the GI hormones to stimulate EGFR tyrosine phosphorylation in NET cell lines."
23220008
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"Consistent with this notion, it has been shown that PDGF-BB and EGF stimulate stronger tyrosine phosphorylation of PDGFR, EGFR, and the downstream Ras-MAPK pathway in cells apposed to ECM than cells i[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
16543902
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"The oncogenic role of miR-24-3p in breast carcinoma has been attributed to its ability to directly target and suppress the expression levels of protein tyrosine phosphatase non receptor type 9 (PTPN9)[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
27939727
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"We used mass spectrometry-based proteomics to comprehensively compare proteins that were tyrosine phosphorylated in response to EGF and PDGF and their associated partners. More than 90% of these signaling proteins were used by both ligands, whereas the phosphatidylinositol 3-kinase (PI3K) pathway was exclusively activated by PDGF,"
15933201
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"These piptides also caused apoptotic cell death, and antagonized EGF-induced phosphorylation of intracellular tyrosine residues in EGFR."
33319463
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"The EGF can induce phosphorylation of all five tyrosine phosphorylation sites of EGFR."
26442853
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"Treatment of UMR cells with epidermal growth factor (EGF), but not PTH, promoted tyrosine phosphorylation of the EGF receptor."
11108712
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"The effects of RC-160 on epidermal growth factor stimulated cell proliferation and tyrosine phosphorylation of epidermal growth factor receptor were examined by colorimetric assay and Western blotting, respectively."
10486467
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"We detected epidermal growth factor (EGF) induced tyrosine phosphorylation of EGF-R not occupied with ligand."
1696547
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"These results indicated that in epithelial cells CD82 and KAI -1 could selectively modulate EGF induced morphological responses related to cellular motility.EGF-induced activation of its receptor resu[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
10985391
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"EGF increased the tyrosine phosphorylation of several proteins (the EGF receptor,insulin receptor substrate (IRS)-1, IRS-2, and Grb2-associated binder 1)"
12138086
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"FLAG-EGFR became tyrosine phosphorylated in response to direct activation with EGF, as well as in response to activation of the â2 adrenergic or LPA receptors"
12627954
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"Second, in plasma membranes, EGF and the polar solvent dimethylsulfoxide (DMSO) selec- tively stimulate tyrosine-residue phosphorylation of the EGF receptor, while serine phosphorylation is unaffe[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
6325268
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"The EGFR ligand, EGF (5 nM), induced EGFR and p185 c-neu tyrosine phosphorylation, but did not result in ErbB3 phosphorylation."
19401448
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"It is known that EGF induces tyrosine phosphorylation and internalization of the EGF receptor in A-431 cells."
11601396
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"To probe Nox4 involvement in regulating EGFR phosphorylation, we initially transfected COS-7 cells with Nox4 and examined EGF stimulated EGFR tyrosine phosphorylation."
18573911
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"It is well established that EGF stimulates the phosphorylation of five major tyrosine residues at the EGFR C-terminus, including Y992, Y1068, Y1086, Y1148 and Y1173."
27463710
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"DiC8 inhibited the EGF dependent tyrosine phosphorylation of the EGF receptor in A431 cells in a similar manner to that observed with PMA."
2981862
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"Based on the observation that EGF induces Tyr phosphorylation of EGFR in the absence of B56 containing PP2As, it has been concluded that B56 containing PP2As regulate the pathway downstream of EGFR."
20934435
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"In DU 145 cells, LHRH-A antagonizes the proliferative action of EGF, inhibits the tyrosine phosphorylation of the EGF receptor induced by EGF, and significantly reduces the number of EGF binding sites."
8923840
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"EGF-induced phosphorylation of EGFR at tyrosine residues was augmented when the cells were pretreated with Y27632 or were subjected to gene silencing using ROCK-siRNA."
21722395
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"We found that both EGF and Cpd 5 induced tyrosine phosphorylation of EGF receptor (EGFR) and ERK."
12115721
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"EGFR tyrosine phosphorylation, however, was incrementally stimulated by EGF concentrations from 1 to 100 ng/ml."
11952645
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"However, the total EGF receptor tyrosine phosphorylation induced by EGF was not enhanced by thrombin prestimulation (not presented)."
11266444
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"we show that exposure to cigarette smoke over the indicated time points or doses results in an increase in total EGFR phosphorylation in a timeand dose-dependent manner (Fig. 1). As with EGF and H2O2, cigarette smoke is able to induce EGFR phosphorylation significantly compared to nontreated cells. Note: human airway epithelial (HAE; A549 or HBE1) were used in the paper"
17971399
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"Suppressing FUT1/4 expression also blocked EGF induced tyrosine phosphorylation of EGFR and mitogen activated protein kinases."
18023290
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"Co-clustering of CD45 and the EGF receptor with specific monoclonal antibodies failed to alter the EGF stimulated tyrosine phosphorylation of the EGF receptor."
1851241
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"The interplay between steroid receptors and EGF receptor results in the up-regulation of EGFR tyrosine phosphorylation by EGF in Cos cells transfected transiently with ERalpha and AR."
28415812
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"In A431 cells, there is spontaneous tyrosine phosphorylation of the EGF receptor that is increased by 1 ng/ml EGF."
7923365
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"Treatment of keratinocytes with TGF-beta suppressed both basal and EGF stimulated EGFR tyrosine phosphorylation, measured by Western blot (XREF_FIG a)."
19864455
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"RB200 inhibited EGF- and NRG1-beta1-stimulated tyrosine phosphorylation of HER1, HER2 and HER3 and also showed potency in cell proliferation assays [XREF_BIBR]."
21982514
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"FLAG-EGFR became tyrosine phosphorylated in response to direct activation with EGF, as well as in response to activation of the â2 adrenergic or LPA receptors"
12627954
sparser
"However, EGCG required neither an ubiquitin ligase (c-Cbl) binding to EGFR nor a phosphorylation of EGFR at tyrosine residues, both of which are reportedly necessary for EGFR degradation induced by epidermal growth factor."
19578043
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"Binding of EGF leads to the up-regulation of EGFR kinase activity, resulting in tyrosine phosphorylation of EGFR itself and its downstream targets (XREF_FIG a and Supporting Information Fig."
25523156
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"We examined the effect of CTTN depletion on EGFR ubiquitination and tyrosine phosphorylation induced by EGF."
27903975
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"Furthermore, siRNA mediated knockdown of RPTP-kappa increased basal and EGF stimulated EGFR tyrosine phosphorylation and augmented downstream Erk activation in human keratinocytes."
16263724
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"As expected, EGF treatment caused EGFR tyrosine phosphorylation, although, interestingly, densitometric analysis of multiple experiments (XREF_FIG) revealed that peak activation was observed in these cells 1 h after EGF exposure."
18762250
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"When the control + EGF sample was probed with an anti-PTyr antibody a large band of the same apparent molecular weight was present suggesting that the EGF receptor was highly tyrosine phosphorylated in response to the EGF treatment."
9722615
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"In all cases, as expected, EGF as a control induced EGF receptor tyrosine phosphorylation in a time dependent manner, while total amount EGF receptor remained unaltered."
11257459
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"We found that both EGF and Cpd 5 induced tyrosine phosphorylation of EGF receptor (EGFR) and ERK."
12115721
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"EGFR tyrosine phosphorylation in response to EGF initiates many signaling events, some of which lead to cell spreading and motility."
12878187
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"The epidermal growth factor (EGF) stimulates rapid tyrosine phosphorylation of EGF-receptor (EGFR)."
19836242
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"Reconstitution of IQGAP1 deficient MEFs with a non phosphorylatable IQGAP1 mutant significantly attenuates the ability of EGF to promote tyrosine phosphorylation of EGFR, while transfection of a phosphomimetic IQGAP1 construct enhances the levels of phosphorylated EGFR observed."
22182509
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"The EGF dependent tyrosine phosphorylation of EGF receptor is blocked by tyrhostin AG1478 and U733122."
11347469
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"we show that exposure to cigarette smoke over the indicated time points or doses results in an increase in total EGFR phosphorylation in a timeand dose-dependent manner (Fig. 1). As with EGF and H2O2, cigarette smoke is able to induce EGFR phosphorylation significantly compared to nontreated cells. Note: human airway epithelial (HAE; A549 or HBE1) were used in the paper"
17971399
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"There are at least three potential mechanisms : (1) LPA may activate a nonreceptor tyrosine kinase that can directly phosphorylate EGF receptor; (2) LPA may inhibit a protein tyrosine phosphatase of E[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12069816
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"EGF (5 nM) induced tyrosine phosphorylation of EGFR which was blocked by gefitinib pre-treatment (1 muM) (XREF_FIG)."
18676863
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"In contrast, EGF stimulation of ECC-1 cells caused tyrosine phosphorylation of EGFR (Y1068), activation of the downstream target ERK, and subsequent EGFR downregulation."
26568478
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"EGF stimulated tyrosine phosphorylation of the EGFR with rapid and transient kinetics."
12749846
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"Of all cell lines examined in our study, mAb ICR62 only inhibited the growth of BxPC-3 cells by 13%, but this was not statistically significant (P = 0.061, XREF_FIG) and blocked the EGF induced tyrosine phosphorylation of EGFR in these cells (XREF_FIG)."
21970876
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"It has been reported that the Tyr residue (position, 1173) in EGFR can be phosphorylated by EGF [ xref ]."
23736792
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"Support for this proposal is provided by our preliminary observations that concurrent treatment of A431 cells for 15 min with EGF and Cur inhibited EGF- mediated activation of EGF-R phosphorylation on[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
7803521
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"Next, we determined whether EGF induces phosphorylation of nuclear EGFR at specific tyrosine residues such as Tyr-845 and Tyr-1065."
19766894
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"Five biological effects were considered : inhibition of high affinity 125I-EGF binding, change in the phosphorylation state of the EGF receptor, inhibition of the EGF dependent tyrosine phosphorylation of the EGF receptor, inhibition of [3H] phorbol 12 beta, 13 alpha-dibutyrate binding, and stimulation of calcium- and phospholipid dependent protein kinase (C-kinase) in vitro."
2985588
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"Treatment with Iressa elicited similar decreases in IL-17E- and EGF induced Y1086-EGFR phosphorylation in the three cell lines and markedly decreased EGFR tyrosine phosphorylation induced by the combination of IL-17E and EGF in BT20 and MDA-MB468 cells and, to a lesser extent, in IJG-1731 cells."
27462789
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"We have thus evaluated the effect of the androgen on EGF mediated signalling pathways leading to invasion in PC3-Neo and PC3-AR cells.By Western blotting analysis, we examined tyrosine phosphorylation[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15288768
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"Therefore, we further examined the effect of pretreatment with AnxA2 antibodies on EGF-induced phosphorylation of individual EGFR tyrosine residues (Tyr-845, Tyr-1045, and Tyr-1068,) in MDA-MB-231 and JIMT-1 cells."
25321192
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"As shown in Fig. 6B, epidermal growth factor stimulation (1 ng/ml, 5 min) of human airway smooth muscle cells resulted in strong tyrosine phosphorylation of epidermal growth factor receptor that was c[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12921856
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"Notably, multiple studies have demonstrated that EGF can also stimulate tumor growth via several mechanisms : phosphorylation of EGFR tyrosine residues, ultimate activation of ERK (extracellular signal regulated kinase) pathways, and possible up-regulation of proliferative genes after intranuclear localisation of phosphorylated EGFR."
28272528
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"In addition, we showed that AR, like EGF or TGF-alpha stimulated the phosphorylation of the epidermal growth factor receptor (EGF-R) on tyrosine residues."
1333777
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"Ras activation by NEU3 was abrogated by PP2 (src inhibitor) or AG1478 (epidermal growth factor receptor (EGFR) inhibitor), and NEU3 actually enhanced EGF stimulated tyrosine phosphorylation of EGFR, suggesting that the upstream targets might be tyrosine kinases including src and EGFR, and the subsequent stimulation of Ras cascade leads to the inhibition of cell apoptosis."
17334392
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"We found that EGF continues to stimulate maximal tyrosine phosphorylation of EGFR following internalization, while, as expected, TGFalpha stimulates markedly less."
10085141
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"Indeed, cells lacking FAK are refractory to EGF- or PDGF induced tyrosine phosphorylation of EGFR and PDGFR, respectively, and cell motility."
19147560
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"In addition, EGF induced the sustained tyrosine phosphorylation of the EGF receptor, mitogen activated protein (MAP) kinases, and 46 and 52 kDa proteins, and the prolonged activation of MAP kinases in PC12h-R cells compared with the parent PC12h, which does not show EGF induced differentiation."
8714524
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"Second, in plasma membranes, EGF and the polar solvent dimethylsulfoxide (DMSO) selec- tively stimulate tyrosine residue phosphorylation of the EGF receptor, while serine phosphorylation is unaffected[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
6325268
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"Lactacystin did not alter the induction of EGF receptor tyrosine phosphorylation, triggered by EGF."
11347469
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"Moreover, EGF stimulation of EGFR tyrosine phosphorylation was not altered by pretreatment with the PKC inhibitor."
9990300
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"EGF can induce phosphorylation of all five known tyrosine phosphorylation sites of EGFR [ xref ]."
25342941
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"EGF stimulation of the transfectants induced dose dependent tyrosine phosphorylation of the EGFR and Jak2 chimera and Stat5, which correlated with the EGF dose dependence of cell proliferation."
8702638
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"In the presence of AS/WAF1, EGF induced the tyrosine phosphorylation of EGF receptor, but WAF1 mRNA was reduced to a half; accumulation of p21 and WAF1 protein and its downstream responses were no longer observed; A431 cells grew continuously."
9488044
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"Both were shown to inhibit growth, although neither of them caused down-regulation of the EGF-R or affected epidermal growth factor (EGF)-induced tyrosine phosphorylation of the EGF-R."
7531976
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"Moreover, EGF stimulation induced a sustained EGFR tyrosine phosphorylation in both cell lines (XREF_SUPPLEMENTARY)."
25062045
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"Curcumin has also been shown as selective inhibitor of phosphorylase kinase [14] and inhibits EGF induced increase in EGF-R tyrosine phosphorylation in a dose- dependent manner [15]."
8913274
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"To this purpose, the effects of LHRH agonist, Zoladex (LHRH-A), on the mitogenic action of EGF, on EGF activated intracellular signaling mechanisms (tyrosine phosphorylation of EGF receptor and c-fos proto-oncogene expression), and on the concentration of EGF receptors have been evaluated in both LNCaP and DU 145 cells."
9396187
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"EGF increased the tyrosine phosphorylation of several proteins (the EGF receptor,insulin receptor substrate (IRS)-1, IRS-2, and Grb2-associated binder 1)"
12138086
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"Next, we determined whether EGF induces phosphorylation of nuclear EGFR at specific tyrosine residues such as Tyr 845 and Tyr 1065."
19766894
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"It was later reported that the pattern and intensity of EGFR tyrosine phosphorylation elicited by AREG or EGF in breast cancer cells were also markedly different, suggesting that differential downstre[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
24463227
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"In KATO-III, a number of tyrosine phosphorylated proteins, including extracellular signal regulated protein kinases (ERKs) and epidermal growth factor receptor (EGFR), were observed irrespective of EGF stimulation and induction of ERK activity and EGFR tyrosine phosphorylation by EGF were less than that in MKN7."
15161014
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"This experiment proved that EGF strongly increased tyrosine phosphorylation of EGF-R without affecting the level of this protein."
7926013
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"We showed that [D-Lys6]GnRH-II reduces EGF-induced auto-tyrosine-phosphorylation of EGF-receptors via activation of a PTP and that EGF-induced activation of mitogen-activated protein kinase was blocked in cells treated with [D-Lys6]GnRH-II."
17016655
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"As shown in Fig. 3 aldosterone and EGF enhanced Tyr phosphorylation of the EGF-R."
11960627
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"Suppressing FUT1/4 expression blocked EGF-induced tyrosine phosphorylation of EGFR and MAPK and inhibited cancer growth (30); Yang et al (21) reported that FUT4 overexpression inhibited cyclophosphamide-induced apoptosis through activating the ERK/MAPK and PI3K/Akt signaling pathways."
26094873
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"EGF can induce phosphorylation of all five known tyrosine phosphorylation sites of EGFR [XREF_BIBR]."
25342941
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"Epidermal growth factor (EGF)-mediated tyrosine phosphorylation of EGFR was decreased in situ in a time- and dose dependent manner in P-Tyr-treated cells."
7679612
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"As depicted in XREF_FIG, 30-min pretreatment with PP2 reduced the level of EGFR tyrosine phosphorylation elicited by EGF applied to nontransfected cells, whereas 90-min incubation with the TKI did not appear to counteract ShcD induced phosphorylation."
24430869
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"As expected, the positive control, EGF, also induced a pronounced phosphorylation of the EGFR at all three Tyr sites."
12915106
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"In contrast, epidermal growth factor (EGF)-mediated tyrosine phosphorylation of the EGF receptor and activation of ERK1/2 were not interrupted by the peptide."
14665433
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"Western blotting with an antiphosphotyrosine antibody showed that TGF-beta1 did not inhibit the EGF initiated tyrosine phosphorylation of the EGF receptor."
9724265
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"We show that all the EGF agonists, but not NDF, stimulated tyrosine phosphorylation of ErbB-1."
8626392
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"The results suggest that PKGII inhibits the proliferation of gastric cancer cells through blocking EGF triggered MAPK signal transduction and the key blocking point is the tyrosine phosphorylation of the EGF receptor."
22012247
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"EGF (20 ng/ml) also increased tyrosine phosphorylation of the EGF receptor (Tyr 1068), as expected."
11728421