IndraLab

Statements

SHC binds EGFR and GRB2. 27 / 27
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"We first describe our strategy to inhibit Grb2/Sos interaction at the level of its SH3 domains and then report the results obtained in blocking Grb2 SH2 domain interaction with EGF-R or Shc."
11007954
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"The Src kinase, Yes, is activated by CCK and mediates EGFR Shc-Grb2 interactions ( xref )."
25761747
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"The concentrations of EGF for which PI 3-kinase inhibitors block Ras activation induce formation of Shc-Grb2 complexes but not detectable EGF receptor phosphorylation and do not activate PI 3-kinase."
10330169
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"By contrast, Shc and Grb2 were inducibly associated with the EGFR in response to EGF stimulation of glioma cells."
10595738
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"Although phosphorylated EGFR can bind to both Shc and Grb2, the predominant linkage was observed between EGFR and Shc."
7798267
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"Grb2 may bind to EGFR with or without Shc."
11583913
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"Additionally, Grb2 and Shc also bind to the activated EGFR via their phosphorylated tyrosine residues, which then serve as an adaptor for the downstream Ras signaling and STAT signaling, respectively ( xref )."
28121014
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"In streptolysin O permeabilised cells, a tyrosine phosphopeptide, EGFR-Y1068P, which binds to the SH2 domain of Grb2, totally blocks growth factor induced formation of complexes between Grb2 and Shc or EGF receptor, and also blocks activation of nucleotide exchange on Ras."
7970708
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"In xref , formation of multi-protein complexes associated with the ErbB1-Shc (panel A), ErbB1-Grb2 (panel B), and ErbB1-Irs-1 (panel C) edges are shown as a function of ErbB1 and Irs-1 expression."
20179207
sparser
"EGFR binding and phosphorylation of Shc and Grb2 initiate this pathway, either by association directly with Grb2, or with a Shc-Grb2 complex (Rozakis-Adcock et al., 1992; Batzer et al., 1994)."
11370743
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"Whereas Grb2 bound to Shc and EGFR in response to EGF, both Shc and Cbl were constitutively associated with the receptor."
11370743
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"By contrast, Shc and Grb2 were inducibly associated with the EGFR in response to EGF stimulation of glioma cells."
10595738
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"An earlier study has shown that Grb2 can interact with EGFR indirectly through Shc ( xref ; xref ), enabling the SH2 domain of Grb2 to interact with other proteins."
19798056
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"The results showed that during mitosis, GRB2 and SHC also bind to EGFR following EGF stimulation ( xref B)."
30544639
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"Sem-5 is homologous to the mammalian protein Grb2, which binds the autophosphorylated EGF receptor and other phosphotyrosine-containing proteins such as Shc through its SH2 domain."
8479540
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"The phosphorylation of these receptors, such as EGFR, also induces the tyrosine phosphorylation of the adapter protein SHC and the association of SHC with Grb2."
22545017
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"Experiments on the EGF receptor, which can also bind directly to either Grb2 or Shc, suggest that both signaling components can contribute to Ras activation34."
8755247
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"Expectedly, PD168393 treatment inhibited serum-stimulated EGFR-Y1086 and AKT phosphorylation and reduced the association of Shc and Grb2 with EGFR."
19935697
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"These analyses reveal a hierarchy of interactions between Grb2 and Shc with the EGFR and indicate that Grb2 can bind the tyrosine-phosphorylated EGFR directly, as well as indirectly via Shc."
7518560
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"The existence of interactions between Shc and Grb2 with the EGFR, with each other, and with a subset of cellular proteins raises the questions of how interactions are controlled: Do all possible inter[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12648464
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"We detected a clear threshold effect in the EGF-induced co-immunoprecipitation (co-IP) between the two proteins, an effect that was not evident when the physical association of EGFR with other known interactors, such as Grb2 or Shc, was analysed ( xref , see also xref for additional experiments relevant to the interaction between Grb2 and the EGFR)."
23799367
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"Notably, activated EGFR interacts with Shc and Grb2 not only at the plasma membrane but also in the endosomal compartment, where EGF induces augmented recruitment of Shc and Grb2."
19489726
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"Surprisingly, EGF and TGF-alpha induced full activation of MAPK in duration and intensity and stimulated association of the EGFR with Shc and Grb2."
11934884
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"After arsenical treatment, Shc immunoprecipitates contained coprecipitated EGFR and Grb2, suggesting that both arsenicals induce the assembly of EGFR-Shc-Grb2 complexes."
14674888
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"Future investigations will assess the precise interaction between IRTKS and EGFR in relation with the EGFR, Shc, and GRB2 complex [46]."
23693078
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"Furthermore, the assembly of EGFR–Grb2 and EGFR–Shc signaling complexes have followed the same pattern ( Figure 3 , middle and bottom panels)."
10985391
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"Accordingly, CGP78850 blocks epidermal growth factor receptor (EGFR)-Grb2 and Shc-Grb2 interactions in living cells."
10471533