IndraLab
Statements
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"1,25(OH)(2)D(3)-regulated expression of the 25-hydroxyvitamin D, 24-hydroxylase (CYP24) gene (both natural gene and promoter construct) was strongly modulated by altering ERK activity (i.e., reduced by MEK inhibitors and dominant negative (dn) ERK1 and ERK2, activated by epidermal growth factor) but ERK inhibition had no effect on 1,25(OH)(2)D(3)-regulated expression of the transient receptor potential cation channel, subfamily V, member 6 (TRPV6)."
reach
"Over-expression of TNIP1 in osteosarcoma (Saos-2) cells [XREF_BIBR] inhibited the nuclear translocation of EGF activated ERK2 recalling the suggestion from Gupta and colleagues [XREF_BIBR] that increased levels of TNIP1 may titrate out cellular factors necessary for its ability to shuttle into the nucleus."
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"In the present study we found that the epidermal growth factor activated ERK2 in cerebellar granule neurons and that this activation prohibited glutamate-induced subcellular translocation of NGFI-B. Likewise, overexpressed active ERK2 resulted in a predominant nuclear localization of green fluorescent protein-tagged NGFI-B. Thus, activation of ERK2 may overcome apoptosis-induced subcellular translocation of NGFI-B. This finding represents a novel and rapid growth factor survival pathway that is independent of gene regulation."
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"Thus, although EGF and PDBu cause comparable maximal activation of ERK2, the PDBu-induced ppERK2 response is relatively sustained ( xref ), and it is associated with very high and sustained nuclear localization of ERK2-GFP in the dephosphorylated form (compare localization of active and total ERK2-GFP in xref )."
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"The overexpression or reduction of IQGAP1 expression by siRNA both caused the activation of Erk2 by EGF or IGF-1 in MCF-7 cells to be reduced, probably due to an optimal level of intracellular IQGAP1 being necessary for maximal level of activation of Erk by EGF or IGF-1 [ xref ]."
reach
"Here we show that in COS cells activation of both endogenous ERK2 and Ras by low, but not high, concentrations of epidermal growth factor (EGF) is suppressed by PI 3-kinase inhibitors; since Ras activation is less susceptible than ERK2 activation, PI 3-kinase-sensitive events may occur both upstream of Ras and between Ras and ERK2."
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"We have investigated the role of Rap1 in regulating 'normal' Ras function by studying the activation of the mitogen-activated protein (MAP) kinases ERK1 and ERK2 by two fundamentally different growth factors, epidermal growth factor (EGF) and 1-oleoyl-lyso-phosphatidic acid (LPA)."
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"It has been reported that transient overexpression of IQGAP1 in MCF-7 breast cancer cells significantly reduced activation of ERK1 and ERK2 by EGF, namely phospho-ERK1/2 was decreased. xref , xref Roy et al xref have reported that maximal activation of MEK and ERK by EGF was observed only when cellular IQGAP1 concentrations were close to normal levels."