IndraLab

Statements


EGF activates MAPK1. 143 / 205
| 4 74 65

reach
"These results suggest that direct or indirect activation of PKA represents a positive regulatory mechanism for EGF stimulation of ERK2 induction."

reach
"Pretreatment of cells with 30 muM TT-232 did not influence subsequent EGF induced ERK2 activation at the early time points, but drastically blocked this event after the 3- to 4-h preincubation."

reach
"The latter conclusion also applies to EGF stimulated expression of cMG1, since the addition of IBMX plus forskolin did not affect the stimulation of ERK-2 by EGF (A.N.C., unpublished data)."

sparser
"Whereas the PH domain was required for Gab1 mediation of ERK2 activation by LPA, it was not essential for EGF-induced ERK2 activation."

sparser
"For instance, EGF-induced activation of Erk2 is mediated by p21'" in PC12 pheochromocytoma cells, in rafl fibroblasts it is mediated by p21'"' and a calcium dependent pathway,[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"As expected, EGF induced pronounced activation of both Erk1 and Erk2."

sparser
"In U343 glioma cells we could now show that SS inhibits the EGF-activated MAPKs ERK 1 and ERK 2 after 5–20 min stimulation time."

reach
"In the presence of actinomycin D (Act D), an inhibitor of RNA synthesis, ERK-2 activity induced by ET-1 and PDGF-BB but not by ET-1 and EGF remained elevated more than sixfold throughout the whole incubation period of 6 h."

sparser
"In all these cells, EGF-induced activation of ERK2 was not affected by the mutant Rac proteins, indicating the presence of functional EGF receptors (data not shown)."

sparser
"EGF-induced Erk-2 activation reached a maximal level at 15 minutes, followed by a decline toward the control level after 3 hours."

reach
"PKC mediated ERK2 activation is associated with prolonged nuclear localization in the dephosphorylated form, whereas EGF stimulated ERK2 activation mediates only transient nuclear accumulation."

reach
"We found that addition of EGF stimulated the activity of ERK2."

reach
"These results show that, in human dermal fibroblasts, EGF stimulates p42mapk and p44mapk isoforms in a protein kinase C independent manner."

sparser
"Overexpressed p74raf-1 potentiates activation of ERK2 by EGF and TPA."

reach
"For instance, EGF induced activation of Erk2 is mediated by p21 ' " in PC12 pheochromocytoma cells, in rafl fibroblasts it is mediated by p21 ' " ' and a calcium dependent pathway, while in Swiss 3T3 [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"On the other hand, either inhibition of PGE2 production with AACOCF3 or H-89, a PKA inhibitor, enhanced EGF induced Erk-2 activity."

reach
"As expected, AG1478 inhibited EGF dependent activation of ERK2."

reach
"Epidermal growth factor (EGF) increased ERK1 and ERK2 associated kinase activity 7-8-fold in the cytoplasm of PGC, while kinase activity of cytoplasmic ERK1 was enhanced 3-4-fold by LH, FSH, or cAMP."

reach
"In keratinocytes, Ca 2+ addition inhibits EGF induced stimulation of ERK2 activity [56] and in mouse embryo fibroblasts chelation of basal intracellular Ca 2+ induces an increased and prolonged ERK1/2[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"EGF-induced ERK2 Activation Promotes β-Catenin Transactivation and Tumor Cell Invasion by Enhancing CK2-phosphorylated α-Catenin at S641."

reach
"In RGM1 cells, EGF significantly increased Ras and ERK2 activities without affecting PKC activity."

reach
"EGF induced Erk-2 activation reached a maximal level at 15 minutes, followed by a decline toward the control level after 3 hours."

reach
"Prolonged activation of ERK2 by epidermal growth factor and other growth factors requires a functional insulin like growth factor 1 receptor."

reach
"In U343 glioma cells we could now show that SS inhibits the EGF activated MAPKs ERK 1 and ERK 2 after 5-20 min stimulation time."

reach
"This EGF induced ERK2 activation was abolished by treatment with the EGF-receptor kinase inhibitor AG1478 (10 (-7) M) or the MEK (MAPK kinase) inhibitor PD98059 (10 (-6) M)."

reach
"Whereas the PH domain was required for Gab1 mediation of ERK2 activation by LPA, it was not essential for EGF induced ERK2 activation."

reach
"In the present study we found that the epidermal growth factor activated ERK2 in cerebellar granule neurons and that this activation prohibited glutamate induced subcellular translocation of NGFI-B."

sparser
"Co-expression of ERK2 with MKP3 wild type resulted in complete abrogation of ERK2 activation by EGF (pidermal rowth actor)."

sparser
"COOK SJ, RUBINFELD B, ALBERT A, MCCORMICK F: RapV12 Antagonizes Ras-Dependent Activation of ERK-1 and ERK 2 by LPA and EGF in Rat-1 Fibroblasts."

sparser
"Cook, S., Rubinfeld, B., Albert, I. and McCormick, F. (1993) RapVl2 antagonizes Ras-dependent activation of ERKI and ERK2 by LPA and EGF in Rat-l fibroblasts."

sparser
"EGF-activated ERK2 binds directly CK2a enhancing its activity (189) ."

sparser
"1,25(OH)(2)D(3)-regulated expression of the 25-hydroxyvitamin D, 24-hydroxylase (CYP24) gene (both natural gene and promoter construct) was strongly modulated by altering ERK activity (i.e., reduced by MEK inhibitors and dominant negative (dn) ERK1 and ERK2, activated by epidermal growth factor) but ERK inhibition had no effect on 1,25(OH)(2)D(3)-regulated expression of the transient receptor potential cation channel, subfamily V, member 6 (TRPV6)."

reach
"In addition, vanadate also unmarked an ET-1- and EGF induced ERK-2 activity after 6 h."

reach
"A short EGF treatment after serum starvation activates MAPK1 as manifested by higher phosphorylation level."

reach
"Nonetheless, these cells were capable of MAPK activation, as underscored by the fact that EGF (1 nmol/L) strongly activated ERK-1 and ERK-2 in COLO-357 cells after a 10-minute incubation."

sparser
"Organization of the cytoskeleton may enable growth-factor-induced activation of endogenous Ras and ERK2 and cell proliferation."

reach
"Fetal lung mesenchymal cells were isolated from E16 Swiss-Webster mice, stimulated with oligopeptide extracellular matrix analogs or anti-integrin antibodies, and subjected to ShcA Western analyses and EGF stimulated Erk1 and Erk2 kinase assays."

sparser
"As expected, AG1478 inhibited EGF-dependent activation of ERK2 ( and )."

reach
"Co-expression of ERK2 with catalytically active PTP-SL in COS-7 cells impaired the EGF induced activation of ERK2, whereas a PTP-SL mutant, lacking PTP activity, increased the ERK2 response to EGF."

reach
"The activated SHP-2 is associated with Gab1 to mediate EGF stimulated ERK2 activation, and Gab1 is the SHP-2 activator for the ERK MAP kinase pathway in EGF stimulated cells XREF_BIBR."

reach
"The addition of either betaVLDL or EGF stimulates a robust activation of ERK1 and ERK2, but the addition of both agents simultaneously sustains the activation for a longer time period."

sparser
"The time courses of activation of ERK2 by Ang II and EGF are shown in ."

reach
"Recent studies indicate that cisplatin up-regulates MAPK pathway and EGF mediated ERK2 activation increases expression of ERCC1 through GATA-1."

sparser
"Expression of Gab1DeltaPI3K had no apparent effect on ERK2 activation by LPA and EGF in the cells that we have examined."

sparser
"Figure 1 shows that mutants Ala-217 and Ala-221 inhibited epidermal growth factor (EGF)-induced activation of ERK2 by 42% and 60%, respectively."

sparser
"At concentrations of 25–100 ng ml, LLZ16402 inhibited JNK and p38 activation by anisomycin but did not inhibit ERK1 and ERK2 activation by EGF ."

reach
"Here, we show that exogenous expression of vinexin beta, a novel focal adhesion protein, allows anchorage independent ERK2 activation stimulated by epidermal growth factor."

reach
"In contrast, EGF activated ERK2 from 10 min to 60 min continuously."

sparser
"Cook SJ, Rubinfield B, Albert I, McCormick F: RapV12 antag- 59. onizes Ras-dependent activation of ERK 1 and ERK 2 by LPA and EGF in Rat-1 fibroblasts."

reach
"Certain cholangiocarcinoma cell lines express several alpha-adrenergic receptor subtypes, and stimulation of the alpha2-adrenoreceptors in vitro up-regulates cAMP, inhibits EGF induced MAPK1 activity, and reduces cell proliferation."

reach
"FAK 925 Tyr P and ERK2 204 Tyr P were adhesion dependent, required an intact actin cytoskeleton, and were mediated by integrins, Flk-1 vascular endothelial growth factor receptors, and epidermal growth factor receptors."

sparser
"4 In the present study, we confirmed that collagen IV can support GEC proliferation, and we showed that EGF can also activate ERK2 in collagen-IV-adherent GECs."

reach
"Finally, comparisons between normal, FAK-null, and FAK reconstituted fibroblasts revealed that FAK enhanced EGF stimulated JNK and ERK2 kinase activation that was required for cell motility."

reach
"These results show that, in human dermal fibroblasts, EGF stimulates p42mapk and p44mapk isofornls in a protein kinase C independent manner."

sparser
"Ectopic expression of ATIP1 in eukaryotic cells leads to inhibition of insulin, basic fibroblast growth factor, and epidermal growth factor-induced ERK2 activation and DNA synthesis, and attenuates insulin receptor autophosphorylation, in the same way as the AT2 receptor."

reach
"In particular, EGF induced activation of Erk2 induces the phosphorylation of Smad1, Smad2, and Smad3, preventing their translocation to the nucleus and thereby interrupting TGF-beta1 signaling [33]."

reach
"Also in these cells, DEL-22379 abolished EGF induced co-immunoprecipitation of ectopic ERK2 molecules tagged with hemagglutinin (HA) or FLAG epitopes, with an estimated half-maximal inhibitory concent[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Consistent with these findings, TPA and EGF also activated the MAPK family members ERK1 and ERK2 measured in the same lysates, and the phosphorylation of these enzymes was also blocked by preincubatio[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Over-expression of TNIP1 in osteosarcoma (Saos-2) cells [XREF_BIBR] inhibited the nuclear translocation of EGF activated ERK2 recalling the suggestion from Gupta and colleagues [XREF_BIBR] that increased levels of TNIP1 may titrate out cellular factors necessary for its ability to shuttle into the nucleus."

reach
"Elevation of cAMP partially inhibited the epidermal growth factor stimulated DNA synthesis and the intrinsic MAPK (ERK-1 and ERK-2) of serum starved MCF-7 cells."

reach
"In- deed, in Swiss 3T3 cells, neither EGF induced activation of ERK2 nor EGF induced leukotriene synthesis is abol- ished by Ras N17, whereas in A14 cells, both insulin- induced activation of ERK2 and[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"In acutely isolated, 90-98% enriched parietal cells, EGF biphasically activated ERK-1 and ERK-2, with peak response occurring at approximately 5 min followed by a sustained lower level of activation for at least 2 h."

reach
"Whereas infection with VV-Ras N17 abolished the insulin induced activation of ERK2, expression of Rac N17 had no effect on either insulin induced or EGF- induced activation of ERK2."

sparser
"In contrast, EGF-induced activation of Erk1, Erk2, and Jun kinase was dependent on Ras but independent of RalA, indicating divergent pathways activated by EGF and mediated by Ras."

reach
"Ectopic expression of ATIP1 in eukaryotic cells leads to inhibition of insulin, basic fibroblast growth factor, and epidermal growth factor induced ERK2 activation and DNA synthesis, and attenuates insulin receptor autophosphorylation, in the same way as the AT2 receptor."

sparser
"In the presence of cytochalasin D, EGF-induced ERK2 activation was inhibited significantly in parental GECs adherent to collagen and was abolished completely in V 12 Ras-transfected GECs on plastic (c[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"ERK2 phosphorylation and activation are generally induced by EGF through EGFR and the cytoplasmic RAS/RAF/MEK pathway, with transient responses observed within a period of less than 1 h [18] ."

reach
"The effects of exogenous PGE2, inhibition of PGE2 synthesis, and modulation of protein kinase A (PKA) activity on EGF induced Erk-2 activation were assessed by immunoprecipitation, kinase assays, and Western blot analysis."

reach
"Kinetic studies showed that EGF stimulated the mobility shift of ERK2 (upper panel) and ERK1 (lower panel) in both concentration- and time dependent patterns."

sparser
"Activation of ERK1 and ERK2 by EGF in these cells occurred rapidly, with significant mobility shifts observed as early as 1 min after treatment (Fig. 4) ."

reach
"AG1478, a specific EGF receptor kinase inhibitor, abolished the prolonged erk-1 and erk-2 activation produced by EGF in cells pretreated with BAPTA/AM."

sparser
"COOK S, RUBINF1ELD B, ALBERT I, MCCORMICK F: RapVl2 Antagonizes Ras-Dependent Activation of ERKI and ERK2 by LPA and EGF in Rat-I Fibroblasts."

sparser
"In the present study we found that the epidermal growth factor activated ERK2 in cerebellar granule neurons and that this activation prohibited glutamate-induced subcellular translocation of NGFI-B. Likewise, overexpressed active ERK2 resulted in a predominant nuclear localization of green fluorescent protein-tagged NGFI-B. Thus, activation of ERK2 may overcome apoptosis-induced subcellular translocation of NGFI-B. This finding represents a novel and rapid growth factor survival pathway that is independent of gene regulation."

reach
"Overexpressed p74raf-1 potentiates activation of ERK2 by EGF and TPA."

reach
"Epidermal growth factor induces phosphorylatlon of extracellular signalregulated kinase 2 via multiple pathways."

sparser
"Regarding Fig. 2A , it is important to note the high basal activity of p44 when compared to p42 (lane 1); the other major finding remains that neither p42 nor p44 kinases can be further activated by [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Activation of both ERK1 and ERK2 by 0.05-10 ng/ml EGF was confirmed when the Westerns were probed with anti-phosphoERK, an antibody that is specific for the phosphorylated forms of ERK1 and ERK2."

sparser
"In- deed, in Swiss 3T3 cells, neither EGF-induced activation of ERK2 nor EGF-induced leukotriene synthesis is abol- ished by Ras N17, whereas in A14 cells, both insulin- induced activation of ERK2[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Second, EGF-activated ERK2 and ERK1 were able to phosphorylate Elk-1 in vitro."

reach
"A very similar situation has been described for growth factor induction of ERK2 MAP kinase in fibroblasts, in which Ras is required for PDGF- and insulin induced, but not EGF induced, activation in Ra[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Both EGF and HGF activated ERK2 significantly, whereas thrombin induced an upward trend in each of the clones ( Table 3 )."

sparser
"These results establish a role for Gab1 in the LPA-induced MAP kinase pathway and clearly demonstrate that Gab1-SHP2 interaction is essential for ERK2 activation by LPA and EGF."

trips
"Ectopic expression of ATIP1 in eukaryotic cells leads to inhibition of insulin, basic fibroblast growth factor, and epidermal growth factor-induced ERK2 activation and DNA synthesis, and attenuates insulin receptor autophosphorylation, in the same way as the AT2 receptor."

reach
"In all these cells, EGF induced activation of ERK2 was not affected by the mutant Rac proteins, indicating the presence of functional EGF receptors (data not shown)."

sparser
"The effects of exogenous PGE2, inhibition of PGE2 synthesis, and modulation of protein kinase A (PKA) activity on EGF-induced Erk-2 activation were assessed by immunoprecipitation, kinase assays, and Western blot analysis."

reach
"Insulin or EGF treatment for 5 min increased p42mapk and p44mapk activity to the same extent as determined by myelin basic protein kinase activity measurements and phosphotyrosine immunoblotting."

sparser
"9,10 By analogy to GECs, a recent study has shown that EGF and platelet-derived growth factor can activate ERK1 and ERK2 more efficiently in NIH 3T3 cells adherent to fibronect[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"In addition, treatment of the cells with the ERK2 activator EGF increased the stability of wild type NGFI-B."

sparser
"In particular, EGF-induced activation of Erk2 induces the phosphorylation of Smad1, Smad2, and Smad3, preventing their translocation to the nucleus and thereby interrupting TGF-β1 signaling [33] ."

sparser
"Thus, although EGF and PDBu cause comparable maximal activation of ERK2, the PDBu-induced ppERK2 response is relatively sustained ( xref ), and it is associated with very high and sustained nuclear localization of ERK2-GFP in the dephosphorylated form (compare localization of active and total ERK2-GFP in xref )."

sparser
"We found that both Tyr-627 and Tyr-659 of Gab1 were required for SHP2 binding to Gab1 and for ERK2 activation by EGF."

sparser
"EGF-activated ERK2 binds directly CK2α enhancing its activity ( xref )."

reach
"On the contrary, calcium treatment inhibited EGF induced stimulation of ERK2 activity."

reach
"Additionally, the blots were also probed with anti-phosphorylated ERK antibody to confirm that EGF treatment of JEG3 cells activated ERK2 and with anti-ERK2 antibody to further confirm equal protein loading."

sparser
"Whereas infection with VV-Ras N17 abolished the insulin-induced activation of ERK2, expression of Rac N17 had no effect on either insulin-induced or EGF- induced activation of ERK2 (Figure 3D)."

trips
"We found that both Tyr-627 and Tyr-659 of Gab1 were required for SHP2 binding to Gab1 and for ERK2 activation by EGF."

reach
"EGF stimulated wild type ERK2 kinase activity increased by 6-fold over that of unstimulated cells and D319N showed an 11-fold increase which A B I D319N I Wild type +/-EGF - + - + D319N ERK2 Wild type[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Both insulin and EGF activated extracellular regulated kinase 2 (ERK-2), p70rsk and protein kinase B (PKB) and inactivated glycogen synthase kinase-3 (GSK-3)."

reach
"Vinexin beta regulates the anchorage dependence of ERK2 activation stimulated by epidermal growth factor."

reach
"Expression of the Gab1 PH domain decoy in these cells suppressed EGF induced Erk2 and Akt and PKB activation, MMP-9 secretion and cell migration."

sparser
"Epidermal growth factor (EGF) also activated p42 and p44MAPK in a time- (peak at 5 min, return to basal within 2 h) and dose-dependent (EC50 approximately 3 ng/ml) fashion."

reach
"As in previous experiments, we found that the time courses for activation of ERK2-GFP by EGF and PDBu were comparable to those for activation of endogenous ERK1/2 (XREF_FIG)."

sparser
"Similarly, EGF-dependent Erk2 activation in the high-density cells was similar to the low-density cells (Fig. 4B) ."

reach
"Dexamethasone significantly suppresses EGF stimulated gastric epithelial cell proliferation and one of the pathways involved is via inhibiting activation of ERK1 and ERK2, followed by inhibition of COX-2, Cyclin D1 expression and DNA synthesis [XREF_BIBR]."

sparser
"As in previous experiments, we found that the time courses for activation of ERK2-GFP by EGF and PDBu were comparable to those for activation of endogenous ERK1/2 ( xref )."

sparser
"In these cells, EGF (and other growth factors) were also able to activate ERK2; however, the moderate expression of V 12 Ras did not independently enhance basal ERK2 activity ( Tables 2 and 3 )."

reach
"Then, EGF promoted the segregation of ERK2 in specific compartments (XREF_FIG) modifying basal ERK2 protein subcellular distribution."

sparser
"The overexpression or reduction of IQGAP1 expression by siRNA both caused the activation of Erk2 by EGF or IGF-1 in MCF-7 cells to be reduced, probably due to an optimal level of intracellular IQGAP1 being necessary for maximal level of activation of Erk by EGF or IGF-1 [ xref ]."

reach
"Whereas EGF caused a greater activation of ERK-2 than insulin, the converse was true for PKB."

reach
"Here we show that in COS cells activation of both endogenous ERK2 and Ras by low, but not high, concentrations of epidermal growth factor (EGF) is suppressed by PI 3-kinase inhibitors; since Ras activation is less susceptible than ERK2 activation, PI 3-kinase-sensitive events may occur both upstream of Ras and between Ras and ERK2."

sparser
"Interestingly, PAI-1 and CTGF induction requires both EGF-activated Erk2 and a synergic interaction between EGFR and TGFβR signaling to phospho-activate SMAD3 [ xref ]."

sparser
"These results suggest that EGF activated ERK2 through endogenous Ras, whereas V 12 Ras was not activating the ERK2 pathway directly, but was activating different downstream effectors that secondarily [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"Nonetheless, these cells were capable of MAPK activation, as underscored by the fact that EGF (1 nmol/L) strongly activated ERK-1 and ERK-2 in COLO-357 cells after a 10-minute incubation ( Figure 10 B[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"EGF-activated ERK2 binds directly CK2α enhancing its activity (189)."

trips
"PKC-mediated ERK2 activation is associated with prolonged nuclear localization in the dephosphorylated form, whereas EGF-stimulated ERK2 activation mediates only transient nuclear accumulation."

sparser
"Growth factor EGF activates ERK2, which phosphorylates NGFI-B and determines if NGFI-B is allowed to translocate to mitochondria."

reach
"Figure 1 shows that mutants Ala 217 and Ala 221 inhibited epidermal growth factor (EGF)-induced activation of ERK2 by 42% and 60%, respectively."

reach
"It was also demonstrated that photo activated hypericin blocked the EGF mediated activation of ERK2, and the inhibition of ERK2 pathway was irreversible."

reach
"PD-98059, a selective inhibitor of the upstream ERK activator mitogen activated protein kinase and ERK kinase, dose-dependently inhibited both carbachol- and EGF stimulated ERK2 activity, with a maximal effect observed between 50 and 100 microM."

reach
"Cells transfected with HA-ERK2 and empty vector were treated with or without EGF (15 ng/ml for 15 rain)."

sparser
"RapV 12 antagonizes Ras-dependent activation of ERKI and ERK2 by LPA and EGF in rat- I fibroblasts."

sparser
"In contrast, EGF activated ERK2 from 10 min to 60 min continuously."

reach
"EGF was reported to increase MAPK1 and MAPK3 associated kinase activity 7-8-fold in the cytoplasm of porcine granulosa cells, while kinase activity of cytoplasmic MAPK3 was enhanced 3-4-fold by LH, FSH, or cAMP [XREF_BIBR]."

sparser
"Activation of both ERK1 and ERK2 by 0.05–10 ng/ml EGF was confirmed when the Westerns were probed with anti-phosphoERK, an antibody that is specific for the phosphorylated forms of ERK1 and ERK2."

sparser
"A very similar situation has been described for growth factor induction of ERK2 MAP kinase in fibroblasts, in which Ras is required for PDGF- and insulin-induced, but not EGF-induced, activation in Ra[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"COOK SJ, RUBINFELD B, ALBERT I, MCCORMICK F: Rap V12 Antagonizes Ras-Dependent Activation of ERKI and ERK2 by LPA and EGF in Rat-1 Fibroblasts."

sparser
"In contrast, overexpression of an IQGAP1 construct lacking the ERK2 binding region did not interfere with activation of ERK1 and ERK2 by epidermal growth factor."

sparser
"In contrast, the D319N mutation had no measurable effect on basal N:C ratio but reduced the translocation response to stimulus, with the increment in N:C ratio caused by EGF activating D319N ERK2-GFP only 50% of that seen with activation of WT ERK2-GFP ( xref )."

sparser
"Notably, the profiles of the temporal and concentration responses of EGF-induced c-fos c-jun mRNA expression were very consistent with those of EGF-induced activation (mobility shift and phosphorylati[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"We have investigated the role of Rap1 in regulating 'normal' Ras function by studying the activation of the mitogen-activated protein (MAP) kinases ERK1 and ERK2 by two fundamentally different growth factors, epidermal growth factor (EGF) and 1-oleoyl-lyso-phosphatidic acid (LPA)."

reach
"We noted that EGF activated ERK1 and ERK2 at significantly lower doses in Huh7.5.1 cells and PHHs."

reach
"Recently, Moroo et al. [41] reported that PACAP activated ERK2 transiently, while EGF activated ERK2 persistently in cultured rat astrocytes, providing a possible explanation for the different actions[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Burgering and co-workers [30], however, showed in NIH 3T3 cells overexpressing the insulin receptor that 8-Br-cAMP inhibited insulin- or epidermal growth factor (EGF)-induced activation of ERK2."

sparser
"These data indicate that a limited degree of adhesion-mediated cytoskeletal organization and focal adhesion complex formation are required for efficient EGF activation of p42 and p44-MAPKs."

trips
"In contrast, overexpression of an IQGAP1 construct lacking the ERK2 binding region did not interfere with activation of ERK1 and ERK2 by epidermal growth factor."

sparser
"In the presence of either PGE2 (50 microg/ml) or 8-CPT-cAMP (100 microM), the EGF-induced Erk-2 activation was lessened."

reach
"EGF induced ERK2 Activation Promotes beta-Catenin Transactivation and Tumor Cell Invasion by Enhancing CK2 phosphorylated alpha-Catenin at S641."

reach
"Activation of both ERK2 and Ras by low concentrations of EGF is suppressed by PI3-kinase inhibitors [XREF_BIBR]."

reach
"In the presence of either PGE2 (50 microg/ml) or 8-CPT-cAMP (100 microM), the EGF induced Erk-2 activation was lessened."

reach
"In contrast, ERK2 was highly activated by EGF treatment, but the magnitude of activation was significantly lower in hepatocytes of aged animals compared to those of young animals (7-fold versus 20-fold, respectively)."

sparser
"Recently, Moroo et al. [41] reported that PACAP activated ERK2 transiently, while EGF activated ERK2 persistently in cultured rat astrocytes, providing a possible explanation for the different actio[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"By analogy to [H]AA release, AG1478 was more effective in inhibiting EGF-dependent ERK2 activation, as compared to activation by complement."

sparser
"It has been reported that transient overexpression of IQGAP1 in MCF-7 breast cancer cells significantly reduced activation of ERK1 and ERK2 by EGF, namely phospho-ERK1/2 was decreased. xref , xref Roy et al xref have reported that maximal activation of MEK and ERK by EGF was observed only when cellular IQGAP1 concentrations were close to normal levels."