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MAP2K1 binds RAF1. 92 / 95
1 16 6 | 2 25 42
reach
"An alternative possibility is that adhesion dependent PAK1 activation and subsequent phosphorylation of MEK1 on S298 is sufficient to stimulate MEK1 phosphorylation and activation by low levels of c-Raf activity merely by enhancing the interaction between c-Raf and MEK1."
12876277
sparser
"Overexpression of activated forms of c-Raf and MEK1 (a component of the mitogen-activated protein kinase pathway, MAPK) led to cell dissociation, as inferred by the loss of desmosomes from the cell periphery."
12045229
reach
"Moreover, cisplatin dissociates cRaf from MEK1 in a dose dependent manner in vitro using purified MEK1 and cRaf complex isolated from KB-3-1 cells by MEK1 immunoprecipitation, suggesting that cisplati[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
30033091
hprd
No evidence text available
8157000
sparser
"We propose that direct interaction of C-Raf and MEK-1 contributes to C-Raf activation by inducing C-Raf hyper-phosphorylation, either by conferring a conformation that is more susceptible to phosphorylation or by stabilizing the phosphorylated form of C-Raf."
23360980
sparser
"NEK10 forms a complex with RAF1 and MEK1 and acts as a positive regulator of ERK1/2 (Extracellular signal-regulated protein kinases 1 and 2) after UV irradiation [ xref ]."
32294979
reach
"We propose that direct interaction of C-Raf and MEK-1 contributes to C-Raf activation by inducing C-Raf hyper-phosphorylation, either by conferring a conformation that is more susceptible to phosphorylation or by stabilizing the phosphorylated form of C-Raf."
23360980
biogrid
No evidence text available
16093354
sparser
"Its expression in tumor cells is further enhanced upon tumor necrosis factor (TNF) alpha treatment, whereas hPEBP4 normally co-localizes with lysosomes, TNFalpha stimulation triggers its transfer to the cell membrane, where it binds to Raf-1 and MEK1."
15302887
reach
"Thus, in this oligodendrocyte enriched glial cell population, Raf-1 can bind to MEK1 in the absence of B-Raf but can not take its place as a MEK kinase or stimulate MEK 's association with KSR, which ultimately results in impaired ERK phosphorylation (XREF_FIG)."
18332218
hprd
No evidence text available
7731720
biogrid
No evidence text available
10757792
sparser
"Delphinidin directly binds with Raf1 or MEK1 noncompetitively with ATP."
19139002
sparser
"We also show that the integrity of the Raf ATP-binding site is necessary for the interaction between Raf-1 and MEK1."
12244094
sparser
"Transfection of the dominant-active form of Raf-1 (Ad-daRaf-1) or MEK1 (Ad-daMEK1) inhibited 1,25(OH)(2)D3-dependent cystatin A promoter activity and its expression."
12682854
reach
"82 In this orthotopic model, the enhanced recruitment of C-Raf and B-Raf to MEK1 was observed."
29170720
biogrid
No evidence text available
10958680
hprd
No evidence text available
8621729
biogrid
No evidence text available
9311917
reach
"This could be because the interaction between CRAF and MEK1 is different than that between BRAF and MEK1 or because DoMY-Seq failed to capture that second binding motif."
33410398
biogrid
No evidence text available
20212043
biogrid
No evidence text available
17979178
sparser
"These results indicated that delphinidin interacts with Raf1 or MEK1, and results in downregulation of their respective kinase activities."
19139002
sparser
"Disruption of the interaction between Raf-1 and MEK1, leads to an inhibition of MEK phosphorylation and activation by Raf-1."
27033522
sparser
"Phosphorylation of MEK1 on serine 298 does not appear to regulate the interaction between Raf-1 and MEK1, but rather the ability of Raf-1 to phosphorylate MEK1 with which it is complexed in vivo."
11948406
biogrid
No evidence text available
21336309
sparser
"In addition, though MEK-1 was able to induce C-Raf phosphorylation at the S338 site, which was shown to play a role in C-Raf activation by certain growth factors [ xref ], MEK-1 was able to bind and activate the S338/339A C-Raf mutant, suggesting that the MEK-1-induced C-Raf activation does not require phosphorylation at these sites ( xref , lanes 4, 5, left panel and lanes 6–9, right panel)."
23360980
sparser
"This result indicates that MEK-1 binding to C-Raf is required for the enhanced C-Raf phosphorylation, suggesting that MEK-1 may confer a C-Raf conformation that is more accessible to phosphorylation or is protected from dephosphorylation."
23360980
reach
"We also found ex vivo binding between delphinidin and Raf1 or MEK1 in JB6 P+ cell lysates (XREF_FIG)."
19139002
sparser
"We optimized DoMY-Seq by taking advantage of the well-described and high-affinity interaction between KRAS and CRAF, and we provide high resolution domain mapping on this and other protein interacting pairs, including CRAF-MEK1, RIT1-RGL3, and p53-MDM2."
33144325
hprd
No evidence text available
16093354
sparser
"Pull-down and competition assays revealed that delphinidin binds with Raf1 or MEK1 noncompetitively with ATP."
19139002
sparser
"Several molecular mechanism underlying this modulation have been proposed including enhanced interaction between MEK1 and Raf-1 [36] , priming of MEK1 for its activation by Raf-1 [37] , promotion of[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18593598
reach
"Furthermore, additional studies suggest that EGCG can also decrease the phosphorylated forms of ERK and mitogen activated protein (MEK1) through its oxidative stress inhibition of the association between Raf-1 and MEK1 and ERK kinase1, leading to suppression of proliferation of the HT29 human colon cancer cell line XREF_BIBR."
25255125
sparser
"In agreement with this, SPR using purified proteins showed that cisplatin binding to MEK1 weakens the interaction of MEK1 with cRaf, but not MAST1 ( xref )."
30033091
sparser
"In the course of studying C-Raf-MEK-1 interactions, we noticed that coexpression of C-Raf with MEK-1 results in a significant mobility shift in C-Raf, suggesting that MEK-1 could induce increased C-Raf phosphorylation [ xref ]."
23360980
sparser
"Conversely, NFAT activity was stimulated by expression of constitutively active forms of c-Raf or MEK1."
11044444
sparser
"Since the dissociation of cRaf from MEK1 induces MAST1 to reactivate the MAPK pathway, any cellular events that affect cRaf-MEK1 binding may activate MAST1-dependent MEK1 activation."
30033091
biogrid
No evidence text available
15485920
reach
"Consistently we found that, although MAST1 is present in the cRaf and MEK1 complex, cancer cells depend on cRaf dependent MEK1 activation to promote proliferation and tumor growth."
30033091
sparser
"DUSP2 rs1724120 [hazard rate ratio (HRR) = 0.72, 95%CI = 0.54, 0.96; AA versus GG/GA), MAP3K10 rs112956 (HRR = 1.40, 95% CI = 1.10, 1.76; CT/TT versus CC) and MAP3K11 (HRR = 1.76, 95% CI 1.18, 2.62 TT versus GG/GT) influenced survival after diagnosis with colon cancer; MAP2K1 rs8039880 (HRR = 2.53, 95% CI 1.34, 4.79 GG versus AG/GG) and Raf1 rs11923427 (HRR = 0.59 95% CI = 0.40, 0.86; AA versus TT/TA) were associated with rectal cancer survival."
23027623
reach
"It has been shown that Raf-1 and B-Raf interact with both MEK1 and MEK2."
11337027
reach
"Specifically, activated Pak1 can phosphorylate Mek1 at Ser298 to enhance the stable association between Raf-1 and Mek1 22, and Pak1 can also phosphorylate Ser338 in the Raf-1 catalytic domain to enhance Raf-1 kinase activity 25."
11435472
sparser
"Pull-down assays revealed that RWE or quercetin (but not resveratrol) bound with either MEK1 or Raf1."
18245498
reach
"Phosphorylation of 338SSYY341 regulates specific interaction between Raf-1 and MEK1."
12244094
reach
"Moreover, the C142A MEK1 neither separated cRaf from MEK1 and cRaf complex nor attenuated MEK activity in cells in response to cisplatin, suggesting that cisplatin binds MEK1 and directly promotes the[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
30033091
bel
"Summary: Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration."
12244094
reach
"PAK1 phosphorylates MEK1 on Ser298, a site important for binding of Raf-1 to MEK1 in vivo."
9351825
sparser
"Of relevance to such possibilities are earlier studies that have shown that Raf1 forms a signaling complex with MEK1, but not MEK2 ( xref ), and that MEK and ERK form a complex together with a scaffold protein that apparently has a preference for MEK1 ( xref ; xref )."
20172001
reach
"Activated Pak phosphorylates Mek1 at Ser298, one of two sites in the catalytic domain thought to be important for stable association between Raf1 and Mek1 7,44,45."
10461188
reach
"Phosphorylation of MEK1 on serine 298 does not appear to regulate the interaction between Raf-1 and MEK1, but rather the ability of Raf-1 to phosphorylate MEK1 with which it is complexed in vivo."
11948406
sparser
"The NEK10 protein acts as a positive regulator of ERK1/2 (Extracellular signal-regulated protein kinases 1 and 2), after UV irradiation and forms a complex with RAF1 and MEK1."
32368190
reach
"The specific structure of cisplatin may disrupt the MEK1 and cRaf complex, resulting in MAST1 as the sole kinase remaining in the complex."
30033091
reach
"We also show that the integrity of the Raf ATP binding site is necessary for the interaction between Raf-1 and MEK1."
12244094
sparser
"The MAPK pathway is activated in differentiating muscle cells and expression of constitutively active form of MEK1 or Raf1 enhances the transcriptional activity of MyoD ."
10640683
biogrid
No evidence text available
15485920
biogrid
No evidence text available
15485920
sparser
"Here we have defined the regions of JSAP1 responsible for the interactions with MEK1 and Raf-1."
11044439
sparser
"In H- ras transformed mouse epidermal cells, EGCG directly causes a decrease in the activation of ERK and MEK1, a decrease in the association of Raf-1 with MEK1, and the inhibition of the AP-1 activity [ xref ]."
19325845
sparser
"In this study overexpression of activated forms of c-Raf and MEK1 led to cell dissociation through the loss of desmosomes from the cell periphery."
22020294
reach
"Disruption of the interaction between Raf-1 and MEK1, leads to an inhibition of MEK phosphorylation and activation by Raf-1."
27033522
reach
"Moreover, the overexpression of MEK1 (3A) failed to block endogenous MEK1 activation, although MEK1 (3A) formed complexes with both c-Raf and B-Raf as well as p42 and p44 MAPK."
9361191
sparser
"RAF1 interaction regulates free β-catenin, but we have also shown that MST2 and LATS1 regulate the RAF1-MEK1 interaction and signalling in a RASSF1A dependent manner [ xref , xref ]."
31963420
sparser
"An alternative possibility is that adhesion-dependent PAK1 activation and subsequent phosphorylation of MEK1 on S298 is sufficient to stimulate MEK1 phosphorylation and activation by low levels of c-Raf activity merely by enhancing the interaction between c-Raf and MEK1."
12876277
sparser
"Because delphinidin can inhibit Raf1 or MEK1 activity, we determined whether delphinidin directly interacts with Raf1 or MEK1."
19139002
reach
"This result indicates that MEK-1 binding to C-Raf is required for the enhanced C-Raf phosphorylation, suggesting that MEK-1 may confer a C-Raf conformation that is more accessible to phosphorylation or is protected from dephosphorylation."
23360980
biogrid
No evidence text available
26496610
sparser
"Two lines of evidence suggest that the MAPK cascade induces the activity of MyoD: 1) the expression of constitutively activated forms of MEK1 or Raf1 enhanced the transcriptional activity of MyoD in 10T1/2 fibroblasts; and 2) the addition of PD098059 to fibroblast cells expressing a conditional MyoD-estrogen fusion protein significantly inhibited the expression of MyoD-responsive genes."
9553102
trips
"PAK1 phosphorylates MEK1 on Ser298, a site important for binding of Raf-1 to MEK1 in vivo."
9351825
sparser
"We optimized DoMY-Seq by taking advantage of the well-described and high-affinity interaction between KRAS and CRAF, and we provide high-resolution domain mapping on this and other protein-interacting pairs, including CRAF-MEK1, RIT1-RGL3, and p53-MDM2."
33410398
reach
"Several molecular mechanism underlying this modulation have been proposed including enhanced interaction between MEK1 and Raf-1 [36], priming of MEK1 for its activation by Raf-1 [37], promotion of a m[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
18593598
sparser
"We note that NIMA gene-family Neks have been previously implicated in the regulation of various aspects of the cell cycle and that Nek-10 is physically associated with Raf-1 and MEK1, formation of the three-protein complex being necessary for Nek-10-mediated MEK1 autoactivation [ xref , xref ]."
25540073
reach
"Active PAK1 (structurally very similar to PAK2) also phosphorylates MEK1, a mammalian MAP2K, at Ser298, which is important for the stable binding of MEK1 and Raf-1 19."
11316611
biogrid
No evidence text available
15866172
sparser
"An isoform of MEK1 was found associated with the complex when Raf-1 is overexpressed [154], and the association of MEK1 with Raf-1 in a complex is dependent on the ac- tivity of p21ras."
8004006
hprd
No evidence text available
11604401
sparser
"Active PAK1 (structurally very similar to PAK2) also phosphorylates MEK1, a mammalian MAP2K, at Ser298, which is important for the stable binding of MEK1 and Raf-1 19 [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
11316611
hprd
No evidence text available
8394352
sparser
"Delphinidin binds with Raf1 and MEK1 and strongly inhibits their kinase activity."
19139002
sparser
"Also, in vitro and ex vivo pull-down assays revealed that delphinidin directly bound Raf1 and MEK1 noncompetitively with ATP, which might account for reduced kinase activities of Raf1 and MEK1."
19139002
biogrid
No evidence text available
10757792
reach
"We previously demonstrated that cisplatin also binds to MEK1 at C142 that mediates disruption of the MEK1-cRaf complex allowing MAST1 to serve as a substitute for cRaf in MAPK signaling ."
34400618
biogrid
No evidence text available
18556463
sparser
"PAK1 phosphorylates MEK1 on Ser298, a site important for binding of Raf-1 to MEK1 in vivo."
9351825
sparser
"This could be because the interaction between CRAF and MEK1 is different than that between BRAF and MEK1 or because DoMY-Seq failed to capture that second binding motif."
33410398
sparser
"This sequence in MEK1 contains phosphorylation sites not present in MEK2 and has been shown to be necessary for MEK1 to interact with C-Raf and for MEK1 activation in cells."
29551131
sparser
"The overexpression of the activated forms of c-Raf and MEK1 leads to cell dissociation."
24009602
biogrid
No evidence text available
19058874
trips
"Through interaction with Raf-1, RKTG changes the localization of Raf-1 from cytoplasm to the Golgi apparatus, blocks EGF-stimulated Raf-1 membrane translocation, and reduces the interaction of Raf-1 with Ras and MEK1."
17724343
reach
"Using an in vitro system, enhanced recruitment of B-Raf and c-Raf to MEK1 occurred only when both MAT2B and GIT1 were present."
25794709
sparser
"This phosphorylation, which occurs in a proline-rich insert in the catalytic domain, has no effect on MEK activity but rather influences the interaction of MEK1 with Raf-1."
10354710
biogrid
No evidence text available
16093354