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TGFB1 activates SERPINE1. 160 / 182
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"In agreement with previous observations, we could demonstrate that TGF-beta1 induced PAI-1 in a time dependent manner in PANC-1 cells, but not in the other cell lines."
12379307
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"Here, cav-1p down-regulated mRNA and protein expression of PAI-1 and alpha-SMA in a dose- and time dependent manners when treated with 10 ng/ml TGF-beta1 for 0-10 muM or 0-48 h."
21440420
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"However, TGF-beta1 upregulated PAI-1 and downregulated uPA in JAR-smad3/c cells, but not in JAR cells."
12074587
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"In a recent study by Kunzmann et al. Progesterone dose-dependently inhibited TGF-b activated Smad signaling and suppressed TGF-b1/Smad-induced upregulation of CTGF, transgelin, and plasminogen activator inhibitor-1 (PAI-1) in lung epithelial cells (21) ."
34912332
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"SB203580 and WP631 inhibited the Smad signal transduction pathway, abrogated the excessive proliferation and reduced the expression of p21 (WAF1 and CIP1) and PAI-1 induced by gamma rays and TGF-beta1 [XREF_BIBR]."
23909719
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"Thus, TGF-beta1 predominantly uses posttranscriptional mechanisms to raise the level of PAI-1 mRNA in astrocytes, whereas bFGF acts at both the transcriptional and posttranscriptional levels."
9798919
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"TGF-beta1 failed to induce PAI-1 synthesis in EGFR deficient fibroblasts while introduction of a wild-type EGFR1 construct in EGFR -/- cells rescued the PAI-1 response to TGF-beta1 confirming, at a genetic level, the targeted knockdown data."
20953304
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"TGF-beta1 increases the synthesis of PAI-1 [XREF_BIBR, XREF_BIBR]."
26000015
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"In NHBE cells, PAI-1 protein is increased by TGF-beta 1 in both extracellular matrix and medium."
2221087
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"TGFbeta1 treatment dramatically increased the secretion of 52-kDa and 42-kDa PAI-1."
17187779
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"Analysis of the sequences mediating TGF-β1-activation of the human PAI-1 gene has shown the importance of three CAGA boxes present in the promoter xref ."
29632840
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"To determine whether OSS generated active TGF-beta1 can induce higher PAI-1 induction, we used an MLEC cell based PAI-1 luciferase assay."
30988341
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"Similarly, TGF-beta1 can up-regulate TIMP-1 and -2, PAI-1 and -2 and deposition of ECM components and adhesive cell surface molecules in human trophoblasts in vitro."
19926662
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"We found that TGF-beta1 stimulation upregulated SERPINE1, CTGF, and ACTA2 mRNA and alpha-SMA protein, and that these effects were significantly reduced in fibroblasts after endoglin knockdown."
33081058
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"miR-130b was down-regulated, whereas TGF-betaR1, as well as the profibrotic genes collagen type IV alpha 1 (Col4a1), Col12a1, CTGF, and PAI-1 were up-regulated not only in mouse MC treated with TGF-beta1 but also in the glomeruli of streptozotocin injected diabetic mice, supporting in vivo relevance."
25204661
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"In A549 cells, TGF-beta1 treatment induced the rapid expression of p21 and PAI-1 mRNAs, and knockdown of FOSL2 significantly attenuated these effects (XREF_FIG)."
25375657
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"We also demonstrated that PAI-1 and Id2 in TR-iBRB2 cells are respectively induced and suppressed by TGF-beta1 (XREF_FIG), which is seen to be secreted from TR-MUL5 cells (XREF_FIG)."
19247458
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"TGF-beta1 has been shown to stimulate the activity of the plasminogen activator inhibitor 1 (PAI-1) in these cells [XREF_BIBR] to effectively suppress MMP activity (e.g. MMP-2)."
23586515
eidos
"Again , the importance of TGF-beta in post-operative adhesion development has been extensively highlighted from in vivo studies and it is well known that TGF-beta1 upregulates PAI-1 production and is a major inducer of collagen deposition ."
| PMC
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"As depicted, TGF-beta1 induced a rapid and potent induction of PAI-1 on the mRNA level, as assessed by qRT-PCR and protein level, as assessed by Western blot analysis."
18337154
trips
"Because the duration of TGF-beta1-induced transcriptional activation of PAI-1 and p21 was shortened in shZEB1-expressing CTLL2 cells, ZEB1 may have a role in enhancing TGF-beta1 signaling by binding not only to Smad3 but also to Smad7 in the nucleus."
20514018
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"Thus, in the node negative population, the upregulation of PAi-1 by TGFbeta1 might constitute an early event that promotes further progression of breast tumours."
16404434
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"In contrast, TGFbeta1 upregulation of PAI-1 is not influenced by ED-A FN, indicating that the PAI-1 gene is regulated differently than alpha-SM actin and collagen type I. Interestingly, it has been shown that the increase of collagen type I and actin mRNA induced by TGFbeta1 is dependent on protein synthesis, whereas the induction of PAI-1 transcript is not."
9700173
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"Again, the importance of TGF-beta in post-operative adhesion development has been extensively highlighted from in vivo studies and it is well known that TGF-beta1 upregulates PAI-1 production and is a major inducer of collagen deposition."
| PMC
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"TGF-beta1 induced synthesis of collagen type II, alphaVbeta1 integrin, and PAI-1."
24252651
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"TGF-beta1 at 50 ng ml -1 promoted the production of N-cad, Vim, FN and PAI-1."
26269426
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"The results showed that the PAI-1 promoter activity was significantly upregulated by DLX1 and TGF-beta1 treatment in a concentration dependent manner (XREF_FIG)."
27593933
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"Furthermore, recombinant human decorin inhibited TGF-b1 induced a-smooth muscle actin (a-SMA), PAI-1 (plasminogen activator inhibitor-1) protein, and mRNA expressions in keloid fibroblasts (P < 0.05)."
25903025
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"Huang et al 7 demonstrated that rennin induced transforming growth factor beta-1 stimulated PAI-1 production."
17175314
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"Comparison of the TGFbeta1 stimulated PAI-1 release from control and IPF lung fibroblasts."
30988425
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"TGF- beta 1 stimulates PAI-1 mRNA in NSCLC and NHBE cells in a persistent fashion."
9294010
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"TGF-beta1 induced PAI-1 mRNA transcripts in quiescent T2 cells via an immediate-early response mechanism."
11719557
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"Activation of canonical TGF-beta1 directly upregulates PAI-1 XREF_BIBR, XREF_BIBR."
29643421
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"The expression of SERPINE1 was upregulated in the progenitors treated with TGF-beta1 for 12h, and it was downregulated in the HPCs treated with TGF-beta1 for 48h, which further confirmed the previous results in the PCR array (XREF_FIG)."
26839553
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"Blockade of TGF-beta1, TGF-beta2, or TGF-beta3 reduced PAI-1 by 29%, 13%, and 16%, respectively."
12911534
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"Besides, TGF beta-1 added to HUVEC induces an upregulation of PAI-1 which is comparable to the upregulation induced by activated platelets."
15935832
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"Addition of authentic TGF-beta1 to parental cells greatly stimulated secretion of PAI-1 but not FN, whereas addition of TGF-beta antibody or lipofection with specific antisense TGF-beta1 oligonucleotides to EGR-1-regulated cells completely inhibits the secretion of PAI-1 but not FN."
9933644
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"XREF_BIBR One of the SMAD3 and SMAD4 binding sequences termed CAGA boxes, which are essential and sufficient for the induction of PAI-1 by TGF-beta1, XREF_BIBR has been identified in the PAI-1 promoter -580 within the FFA responsive region."
19752858
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"These results suggest that excessive TGF-beta 1 stimulated PAI-1 could prevent appropriate peritoneal healing by impairing the degradation of fibrin and of unorganized matrix components, and by interfering with cell migration."
9648067
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"These data indicate that cellular adhesive state modulates TGF-beta1 signaling to particular target genes (i.e., PAI-1) and that MEK is a critical mediator of the PAI-1 (+)/promigratory phenotype switch induced by TGF-beta1 in T2 cells."
11719557
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"The upregulation of PAI-1 by TGF-beta1 has been reported to involve non canonical (smad independent) pathways such as MAPK activation of the transcription factor AP-1 45."
31424116
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"SerpinE1 (PAI-1) was upregulated 6.8-fold by TGFbeta2 treatment of glaucomatous TM cells and by TGFbeta1 in HTM cells."
19087875
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"PPARgamma activation is associated with reduced expression of ECM proteins and TGF-beta1 in the glomeruli of STZ induced diabetic rats XREF_BIBR XREF_BIBR and may directly attenuate diabetic glomerular disease by inhibiting PAI-1 expression XREF_BIBR."
27184517
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"In a previous study (17), we found that TGF-beta 1 significantly enhances the release of PAI-1 from cultured endometriotic stromal cells but not from endometrial stromal cells from women with endometr[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
15831288
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"The rapid activation of p42/44 MAP kinase (MAPK) by TGF-beta1 was eliminated completely, and transcriptional activation by TGF-beta1 of the plasminogen activator inhibitor-1 gene was reduced by 50% in both endothelial cells and Mv1Lu when they were infected with AdCARasY57."
10447679
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"Using the standard protocol from Abe et al. [25] with a short plating time of 4 hr, serial dilutions of TGFbeta1 induced the PAI and Luc construct with a dose dependent fashion."
11841786
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"In DU-145 cells, only TGF-beta1 was an effective inducer of PAI-1."
23541763
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"IL-1beta, Tgfb1, and PLAU all had increased expression in Serpine1 -/- lungs at day 4 relative to B6 controls but decreased expression at day 7, while Ptgs2 and Tnf had decreased expression in the Serpine1 -/- mice at both time points."
23919993
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"All-trans retinoic acid inhibits the increases in fibronectin and PAI-1 induced by TGF-beta1 and Ang II in rat mesangial cells."
18718172
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"Lack of Smad2 or Smad3 expression did not affect TGF-beta1-induced fibronectin synthesis but resulted in markedly suppressed induction of plasminogen activator inhibitor-1 by TGF-beta1."
11262418
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"XREF_BIBR, XREF_BIBR Moreover, TGF-beta1 significantly increased the expression of all 3 fibrosis related pathway genes (C-myc, Pai-1, and Ctgf; XREF_FIG)."
31943022
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"As shown in Fig. 8A , PAI-1 was the most significantly and most dramatically induced by TGF-beta1 , accompanying up-regulation of chemokines Ccl2 and Ccl7 , signalling mediators Cyr61 ( Ccn1 ) , Ctgf ( Ccn2 ) and Tsku , and collagen crosslinking enzyme Plod2 ."
33149203
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"Since TGF-beta1 increases PAI-1 at the transcription level, these results indicated that alpha-LA inhibits TGF-beta1-induced PAI-1 expression through inhibition of TGF-beta1 expression [XREF_BIBR]."
26799284
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"Activation of TGF-beta1 upregulates PAI-1 19."
24549864
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"TGF-beta1 upregulates PAI-1 gene and protein levels in wild type tendon cells."
24962629
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"IL-1 , Tgfb1 , and PLAU all had increased expression in Serpine1 Gangia / Gangia lungs at day 4 relative to B6 controls but decreased expression at day 7 , while Ptgs2 and Tnf had decreased expression in the Serpine1 Gangia / Gangia mice at both time points ."
23919993
eidos
"IL-1beta , Tgfb1 , and PLAU all had increased expression in Serpine1 - / - lungs at day 4 relative to B6 controls but decreased expression at day 7 , while Ptgs2 and Tnf had decreased expression in the Serpine1 - / - mice at both time points ."
23919993
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"Herein we demonstrate that PAI-1 levels are increased in the active EoE esophagus as compared with inactive EoE, athat TGFbeta1 significantly induces PAI-1 in esophageal epithelial cells, and that pharmacologic inhibition of PAI-1 decreases TGFbeta1 induced gene expression."
27212082
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"Indeed, up-regulation of caveolin-1 appears to stall cells in G 0 / G 1 via activation of the p53 and p21 cell cycle arrest pathway and restoration of caveolin-1 in caveolin-1-deficient cells rescues TGF-beta1 inducibility of the PAI-1 gene."
31382626
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"Furthermore, the antisense ODN administration reduced the elevation of PAI-1 protein stimulated by TGF-beta1, both intracellular and extracellular."
15135656
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"TGF-beta1 stimulation of the PAI promoter was inhibited when Smad7 was overexpressed, as has previously been reported [8]."
23567938
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"It has been confirmed that TGF-beta1 induces PAI-1 synthesis, and a TGF-beta1 response element has been found in the promoter of the PAI-1 gene; in addition, multiple lines of evidence have determined that PAI-1 expression might be regulated in connection with several signaling pathways."
| PMC
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"We found that the PAI-1 gene, Serpine1, (PAI-1 gene) was significantly upregulated 2 to 6-fold by TGF-beta1 under almost all the conditions tested, while PAI-1 protein levels were significantly elevated 12 to 25-fold by TGF-beta1."
24962629
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"Our results demonstrate that protoporphyrins can block the TGFbeta1 mediated induction of PAI-1 protein in VSMCs and that this inhibitory effect is independent of HO activity."
18344023
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"We used esophageal biopsy specimens and plasma samples from control subjects and patients with EoE, primary human esophageal epithelial cells, and fibroblasts from patients with EoE in immunohistochemistry, quantitative PCR, and immunoassay experiments to understand the induction of PAI-1 by TGF-beta1, the relationship between PAI-1 and esophageal fibrosis, and the role of PAI-1 in fibrotic gene expression."
27212082
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"These results indicate that TM5275 has potential to suppress HSC-T6 cell proliferation by inhibiting the upregulation of PAI-1 stimulated by TGF-beta1."
| PMC
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"In keeping with previous studies investigating the effects of TGF-beta1 on lung fibroblasts, 16 TGF-beta1 induced transcriptional activation of JunB, PAI-1, and CCN1 but not CCN3 in a time dependent m[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
19116361
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"As Fig. 2C shows, TGF-beta 1 stimulates PAI-1 promoter activities in cells harboring EV."
12044892
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"Connective tissue growth factor (CTGF) and plasminogen activation inhibitor 1 (PAI-1) were also induced by TGF-beta1 (CTGF : 21.73 +/-1.54; PAI-1 : 54.40 +/-5.48; p < 0.05) and are thought to play important roles in pulmonary fibrosis [XREF_BIBR, XREF_BIBR]."
24762191
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"Transforming growth factor beta 1 (TGF-beta1) induced a significant dose dependent increase of PAI-1 release into the medium."
10831181
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"22 Additionally, PAI-1-/- keratinocytes have been shown to lose their ability to migrate in an in vitro scratch assay, while TGF-beta1 has been shown to stimulate the attachment and invasion of cells [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
21798735
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"Similarly, PAI-1 induction in renal and non renal cells by TGF-beta1 requires EGFR-ERK1/2 signaling and a USF1 --> USF2 isoform switch at the PE2 E-box site in the PAI-1 promoter."
21638209
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"By Western blot and RT-PCR analyses, TGF-beta1 was shown to induce a strong increase of PAI-1 synthesis."
14597403
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"TGF-beta1 up-regulates plasminogen activator inhibitor type 1 (PAI-1), which lowers matrix degradation."
16316341
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"TGF-beta1 induces uPA and PAI-1 secretion and promotes binding of uPA at the external plasma membrane with increased membrane associated plasmin activity."
10652434
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"Rhein inhibits transforming growth factor beta1 induced plasminogen activator inhibitor-1 in endothelial cells."
12781036
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"XREF_FIG, TGF-beta1 significantly elevated PAI-1 expression in keloid fibroblasts, while both AA (30 muM) and Ros (2 muM) suppressed the PAI-1 upregulation induced by TGF-beta1 in keloid fibroblasts, which was abrogated by GW9662 (1 muM)."
24250248
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"ASODN 1, 7, 8, 14, and 15, among twenty seven designed ASODNs against PAI-1 mRNA, significantly decreased PAI-1 antigen and PAI-1 activity induced by TGF-beta1 in a dose dependent manner in HUVEC in vitro."
16867253
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"Fig. 2B shows that Smad4wt-2 cells express a higher amount of PAI-1 than control cells and that TGF-beta 1 was able to increase the production of PAI-1 in both control and Smad4wt cells."
12044892
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"Furthermore, MKK3 deficiency resulted in a selective disruption of TGF-beta1-stimulated up-regulation of pro alpha1 (I) collagen expression but not TGF-beta1 induction of fibronectin and PAI-1."
12374793
eidos
"Serpine1 expression is promoted by TGFbeta1 ( Honda et al ., 2017 ; Samarakoon & Higgins , 2008 ) and it is the major physiological regulator of the plasmin based cascade which is involved in fibrotic disorders of the vascular system and several organs such as skin , liver , lung and kidney ."
31820438
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"Consistent with suppression of SMAD3 phosphorylation, PAI-1 induction by TGF-beta1 is also effectively attentuated by NAC preincubation (XREF_FIG, XREF_FIG)."
21829547
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"Since TGFbeta1 induced PAI-1 in human esophageal epithelial cells and PAI-1 was elevated in EoE, we determined whether PAI-1 and TGFbeta1 expression correlated with one another."
27212082
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"Ac-SDKP inhibited TGF-beta1 induction of increased PAI-1 and alpha2 type I collagen in vitro in mesangial cells, linked to inhibition of Smad2 phosphorylation."
19273183
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"SB203580 and WP631 can abrogate excessive proliferation, expressions of p21 (WAF1 and CIP1) and PAI-1 induced by gamma rays and TGFbeta1 in HLFs through blocking Smad signal transduction pathway."
18606061
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"Higher expression of TGFbeta1 inducible early responsive gene in decorin-null livers indicated enhanced bioactivity of TGFbeta1 known to upregulate TIMP-1 and PAI-1, as well."
20956977
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"TGF-beta1 strongly induced PAI-1 mRNA and protein levels, occurring in a manner that was abolished by pretreatment with DHT (XREF_FIG)."
18829577
eidos
"The upregulation of PAI-1 by TGF-beta1 has been reported to involve non-canonical ( smad-independent ) pathways such as MAPK activation of the transcription factor AP-1 45 ."
31424116
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"The strong induction of PAI-1 by TGF-beta1 and the concomitant inhibition of plasminogen activators may play a protective role during the earliest stages of tumor progression."
14597403
eidos
"TGF-beta1 , the major fibrogenic molecule involved in the mechanisms of excessive ECM deposition in pulmonary fibrosis , negatively regulates PTX3 and ECM-degrading molecules ( e.g ., MMPs , uPA ) and up-regulates TIMPs and PAI-1 ( 45 , 75 ) ."
34276664
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"Aldosterone and TGF-beta1 synergistically increase PAI-1 and decrease matrix degradation in rat renal mesangial and fibroblast cells."
18367662
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"In this study, we show that TGF-beta1, a most ubiquitous and potent profibrotic cytokine, induces plasminogen activator inhibitor 1 (PAI-1), a cell senescence and fibrosis mediator, and p16 as well as senescence, but not apoptosis, in primary mouse ATII cells."
31513752
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"atRA pretreatment effectively reduced fibronectin expression in glomerular mesangial cells stimulated with TGF-beta 1 or Ang II for 48 h. TGF-beta 1 stimulated PAI-1 expression reached a maximum at 5 h. atRA did n't affect the early (5 h) PAI-1 induction by TGF-beta 1, but markedly attenuated the sustained (48 h) PAI-1 induction."
18718172
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"A previous study also indicates that PAI-1 mRNA is also upregulated in NRK52E cells treated with TGF-beta 1 XREF_BIBR."
25056456
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"It has been found that CBP/P300 I1395G mutant (only having KCT activity but not KAT activity) can replace endogenous CBP/P300 KCT to crotonylate promoter and enhance intracellular transcriptional activation, thus improving TGF-β1-induced activation of PAI1 and SMAD7 [ xref ]."
34059135
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"For further validation, downregulation of E-Cadherin, upregulation of N-Cadherin and Pai1 by TGF-beta1 in control and Caveolin-1 knockdown hepatocytes were assessed on protein level (XREF_FIG)."
23328673
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"Furthermore, previous studies have demonstrated that PAI-1 may be upregulated by TGF-beta1 and VEGF."
29328420
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"TGIF Protein Suppresses PAI-1 mRNA Up-regulation Induced by TGF-beta1."
19524896
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"A novel purification procedure was established, which results in the isolation of highly active and detergent-free TGF beta 1 induced PAI-1."
2190800
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"Together, our data demonstrate that TGFbeta1 increased the mean ratio of uPA to PAI-1, and ILK knockdown decreased such ratio (control, 1.0; TGFbeta1, 2.69; ILK-H control, 0.45; and ILK-H plus TGFbeta[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
17187779
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"PAI-1 was augmented by TGF-beta1."
26269426
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"TGF-beta1 induced a strong increase of PAI-1 mRNA in PrEC and PC-3 cells, moderate increase in DU-145 and no effect in LNCaP cells."
23541763
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"In accordance with the immunofluorescence data, the co-culture of MAECs with WT BMDMs caused the downregulation of EC related genes (Pecam, Kdr, and Col4a2) and the upregulation of mesenchymal genes (Tagln and Acta2) and the direct TGFbeta1 target Serpine1 (Pai1)."
33063665
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"XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR - XREF_BIBR In this regard TGF-beta1 mediated synthesis and subsequent secretion of PAI-1 can modulate secondary signaling events (ERK or Akt activation) that prevent endothelial cell apoptosis or induce survival programs that precede and necessary for efficient morphogenesis response Pharmacological blockade of ERK1/2 and EGFR, also virtually eliminates endothelial branching induced by TGF-beta1 in keeping with signaling elements necessary for TGF-beta1 mediated PAI-1 induction and subsequent endothelial morphogenesis."
20953304
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"Two other genes, Pai1 and Ctgf, whose expression is normally stimulated by TGF-beta1, displayed no increase in the Tgfb1 -/C33S mutants, consistent with lack of an increase in active TGF-beta1 in the mutant genotypes (XREF_FIG)."
24590056
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"Both TGF-beta1 and H (2) O (2) increased fibronectin and PAI-1 secretion in dose dependent manners."
22483454
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"By contrast, TGFbeta1 stimulated a dose dependent release of PAI-1 by confluent cells which appeared to be independent of media p O 2; release was greatest at 20 ng/ml TGFbeta1.At hypoxia, both FGF2 a[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
11311855
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"TGF-beta1 induces plasminogen activator inhibitor 1 (PAI-1) synthesis and is associated with BMI in humans XREF_BIBR, XREF_BIBR."
23826157
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"TGF-beta1 inhibition reduces PAI-1 in stem and progenitors."
24223881
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"Flexor tendon cells from wild type and PAI-1 KO mice were treated with and without TGF-beta1 in the presence of supplemented plasminogen and tPA."
24962629
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"And ILK-H further increased the TGFbeta1 stimulated secretion of 52-kDa and 42-kDa PAI-1 and an additional PAI-1 immunoreactive band of approximate 70 kDa."
17187779
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"Finnson et al. (2008) showed that COL2 and PAI-1 is stimulated by exogenous TGF-beta1 in chondrocytes."
25621374
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"In vitro, TGF-beta1 enhances PAI-1 production in glomerular mesangial cells [14]."
17328891
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"In summary, our work suggested that TGF-β1-induced SERPINE1 activation contributed to hemin-induced neuronal apoptosis and inflammation, providing novel evidence for the linkage between TGF-β1-induced SERPINE1 activation and ICH progression."
33603380
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"Fig. 1 B showed that PAI-1 promoter activity was induced 4.3-fold by TGF-beta1."
17328891
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"TGF-beta 1 increased PAI-1 mRNA in a time- and concentration dependent manner."
9648067
sparser
"Notably, SERPINE1 knockdown attenuated the effect of TGF-β1 on hemin-induced neuronal damage, supporting the notion that TGF-β1-induced SERPINE1 activation is implicated in hemin-induced neuronal apoptosis and inflammation in HT22 cells."
33603380
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"Using a well established reporter assay (where luciferase runs off the promotor of the TGFbeta1 target SERPINE1 (PAI-1; plasminogen activator inhibitor-1) 24), we were able to show that VA (aq) was able to inhibit active TGFbeta1 function."
| PMC
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"We found that p53 knockdown in MCF10A cells, which express similar levels of p63 and p73 as control cells (XREF_SUPPLEMENTARY), did not impede TGFbeta1 induced growth arrest or p21 or PAI-1 mRNA activation (XREF_FIG; data not shown)."
23706820
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"Clinical implications Because PAI-1 induced by TGF-beta1, as expressed in epithelial cells from patients with EoE, correlates positively with fibrosis and its inhibition decreases TGF-beta1-induced fi[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
27212082
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"Renin induced increases in TGF-beta1 in turn stimulate increases in PAI-1, FN and collagen I. Thus, renin may contribute to renal fibrotic disease, particularly when therapeutic Ang II blockade elevates plasma renin."
16374430
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"In addition, the TGF-beta 1 -induced transcriptional activation of PAI-1 is unaltered in these cells by the manipulation of LRP-1."
15044004
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"Thus, the induction of PAI-1 by TGF-beta1 may lead to inhibition of protease dependent proteolytic activity and accumulated deposits of ECM, resulting in glomerulosclerosis."
17328891
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"TGFbeta1 induced PAI-1 is involved in TGFbeta1 induced gene expression."
27212082
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"TGFbeta1 Induced PAI-1 Contributes to a Pro Fibrotic Network in Eosinophilic Esophagitis."
27212082
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"TGFbeta1 activates PAI-1 and PAI-1, in turn, stimulates TGFbeta1 26."
28396839
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"TGF-beta 1 also caused an increase of both u-PA and PAI-1 antigens, while there was no detectable effect on t-PA."
1655420
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"We used esophageal biopsy specimens and plasma samples from control and EoE subjects, primary human esophageal epithelial cells, and EoE fibroblasts in immunohistochemistry, quantitative PCR, and immunoassay experiments to understand the induction of PAI-1 by TGFbeta1, the relationship between PAI-1 and esophageal fibrosis, and the role of PAI-1 in fibrotic gene expression."
27212082
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"SB203580 and WP631 inhibited the Smad signal transduction pathway, abrogated the excessive proliferation and reduced the expression of p21 (WAF1 and CIP1) and PAI-1 induced by gamma rays and TGF-beta1 [XREF_BIBR]."
| PMC
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"Transforming growth factor-beta1 (TGF-beta1) increased PAI-1 secretion in a dose- and time dependent manner."
10845889
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"TGF-b1, the major fibrogenic molecule involved in the mechanisms of excessive ECM deposition in pulmonary fibrosis, negatively regulates PTX3 and ECM-degrading molecules (e.g., MMPs, uPA) and up-regulates TIMPs and PAI-1 (45, 75) ."
34276664
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"As TGF-beta1-mediated upregulation of PAI-1 expression has been implicated in numerous tissue fibroses, we hypothesize that upregulation of PAI-1 by TGF-beta1 in tendon cells suppresses important proteases involved with ECM turnover and degradation, namely plasmin and MMPs."
24962629
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"TGF-beta1 has been shown to be a strong inducer of CTGF and PAI-1 in animal models of cardiac remodeling39 ."
36324284
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"ACT-333679 prevented TGFbeta1 induced fibroblast-to-myofibroblast transition, proliferation, ECM synthesis, IL-6 and PAI-1 secretion and exerted relaxant effects in cell contraction assays."
30537446
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"Collagen-1A2, matrix metalloproteinase-9, and WNT11 were upregulated, and only SERPINE1 appeared to be downregulated in the HPCs treated with TGF-beta1 for 48h (XREF_TABLE)."
26839553
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"The elevated expression of GST-P was significantly reduced in the CASE (120 and 240 mg/kg)-treated groups compared to the DEN group both at 12th week and 16th week.As illustrated in Fig. 10, TGF-beta1[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
24247078
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"TGFbeta1 increased the release of PAI-1 from endometriotic cells (p < 0.05) but had no effect in endometrial cells."
12624545
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"As expected, TGF-beta1 induced a concentration dependent increase in PAI-1 release in both control and IPF fibroblasts that plateaued at ~ 10ng/ml and ~ 3ng/ml, respectively."
30988425
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"In addition, since PAI-1 is robustly induced by TGF-beta1 signaling, we examined its upstream regulatory activity."
31424116
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"p53 and SMAD3 interactions and complex formation were previously implicated as critical for TGF-beta1 mediated PAI-1 promoter activation."
25810340
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"TGFbeta1 induced the PAI and Luc construct in a dose dependent fashion but its effect diminished when added to cells previously cultured for 24 and 48 hr."
11841786
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"Furthermore, TGF-beta1 induces plasminogen activator inhibitor 1 (PAI-1) synthesis and stimulates the synthesis of collagen and alpha-actin in vascular smooth muscle cells XREF_BIBR."
25988317
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"Thrombospondin (TSP) and transforming growth factor beta 1 (TGF-beta) promote human A549 lung carcinoma cell plasminogen activator inhibitor type 1 (PAI-1) production and stimulate tumor cell attachment in vitro."
8093068
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"Our findings support the hypothesis that TGF-beta1 induces PAI-1, which suppresses plasmin and plasmin mediated MMP activity, and provide evidence that PAI-1 may be a novel therapeutic target for preventing adhesions and promoting a scarless, regenerative repair of flexor tendon injuries."
24962629
sparser
"Consistent with this requirement, PAI-1 promoter activation by TGF-β1 was effectively blocked by SIS3 ( xref ) in mink lung epithelial (Mv1Lu-800bp-Luc) cells engineered to stably-express a luciferase reporter driven by an 800bp fragment of the human PAI-1 promoter [ xref ]."
24613410
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"Progesterone also antagonized the TGF-beta1 and Smad-induced upregulation of CTGF, transgelin, and PAI-1 at the promoter, mRNA, and/or protein levels."
30001393
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"Further, PAI-1, which is induced by TGF-beta1 activation of the Alk5 and SMAD2/3 pathway, contributes to vessel stabilization by preventing degradation of the provisional matrix deposited around new vessels and favoring the establishment of new basal lamina, again consistently with our finding that PAI-1 was specifically and robustly induced in the low VEGF conditions, leading to the fastest stabilization."
26323572
eidos
"It has been confirmed that TGF-beta1 induces PAI-1 synthesis , and a TGF-beta1 response element has been found in the promoter of the PAI-1 gene ; in addition , multiple lines of evidence have determined that PAI-1 expression might be regulated in connection with several signaling pathways ."
| PMC
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"Albo, D., Arnoletti, J. P., Castiglioni, A., Granick, M. S., Solomon, M. P., Rothman, V. L. & Tuszynski, G. P. (1994) Thrombospondin (TSP) and transforming growth factor beta 1 (TGF-l) promote human A[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
7585661
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"Since TGF-beta1 is well known to stimulate the PAI-1 promoter, we suggest that TGF-beta1 and PAI-1 together constitute a positive feedback loop in the development of renal fibrosis in diabetes."
19786738
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"To further confirm this, we detected the PAI-1 and Smad 7 mRNA level in glioblastoma cells treated with TGF-a1, the data showed that PAI-1 mRNA was increased significantly in four cells treated with TGFb1 for 48h, however Smad7 mRNA was not enhanced as highly and significantly as PAI-1 mRNA."
28912531
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"Consequently, expression of tissue factor, urokinase receptor, and PAI-1 mRNA and PAI-1 protein secretion induced by busulfan were significantly reduced by the activin A/TGF-beta 1 inhibitor SB 431542 in ECV304 and primary endothelial cells.Conclusions This is the first report that directly relates busulfan exposure to antifibrinolytic activity by PAI-1 and hypercoagulation possibly mediated by members of the TGF-beta1 family."
22286157
sparser
"TGFβ1 activates PAI‐1 and PAI‐1, in turn, stimulates TGFβ1 xref ."
28396839
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"We further demonstrate that TGFbeta1 increases PAI-1 in primary human esophageal epithelial cells and EoE fibroblasts."
27212082
sparser
"Interestingly, CAGA is also recognized to mediate binding to Smad3 and Smad4, and are required for TGF- β -induced transcriptional activation of the plasminogen activator inhibitor-1 promoter. xref Later studies have suggested that in addition to the GTCT and AGAC elements, Smad4 is also able to recognize a GC-rich sequence. xref , xref It is important to note that Par-4 promoter sequence is extremely GC-rich."
24503536
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"In primary cultured peritoneal mesothelial cells, TGF-beta 1 also induced PAI-1 secretion and the shift of tPA toward high molecular weight complexes."
9648067
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"Using cultured glomerular mesangial cells, we confirmed that TGF-beta1 induces cellular ROS, upregulates PAI-1 mRNA and protein expression, and suppresses plasmin activity."
13679067
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"Similarly, TAK1 (K63W), previously proved to be another dominant negative inhibitor for TAK1 [9], also inhibited PAI-1 induction, supporting that TAK1 inhibition leads to inhibition of PAI-1 productio[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
12859960
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"Interestingly, Smad4dn Car C cells express a lower promoter activity (compared with control cells) and PAI-1 is not stimulated by the addition of TGF-beta 1."
12044892
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"Evidently, TGF-beta1 promotes the production of N-cad, Vim, FN and PAI-1."
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