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TGFB1 increases the amount of SMAD3. 60 / 68
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"Meanwhile, the immunofluorescence staining result also showed that TGF-beta1 increased the expression of pSmad3; on the contrary, curcumin decreased the expression of pSmad3 and inhibited nuclear translocation of pSmad3 (XREF_FIG)."
28203261
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"Furthermore, the levels of p-Smad3 and collagen I induced by TGFbeta1 were slightly increased after the addition of the recombinant human SDC-1 protein to Beas-2B and HLF-1 cells."
34671356
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"TGF-beta1 also induced Smad3, miR-192 and p-Smad3 expression, but suppressed E-cadherin expression."
23588932
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"As shown in XREF_FIG, western blotting results demonstrated that TGF-beta1 significantly increased the levels of Smad-2 phosphorylation and moderately enhanced Smad-3 phosphorylation."
25435153
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"The results showed that TGF-beta1 induced the expression of CTGF, COL I, p-Smad3 and Smad7 in a time dependent manner."
23271281
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"However, the selective inhibitor of the TGF-beta1 and Smad signaling pathway, SB431542, significantly decreased the protein expression of TGF-beta1, p-Smad2 and Smad2, p-Smad3 and Smad3 and Smad7 in NFs."
33456506
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"In the same series of experiments, we observed that endogenous TGFbeta1, like RAC1B [XREF_BIBR], promoted the expression of SMAD3 (XREF_FIG)."
33260366
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"Our findings revealed that the transfection of TGF-beta1 promoted the expressions of TGF-beta1 and Smad3, playing a protective role in ischemic stroke, and this protective role was illustrated by reduced infarct size."
28110342
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"The administration of TGF-beta1 increased the migration and invasion of EVI5-knockout NSCLC cells, and increased the levels of p-Smad3 and its downstream signaling molecules, indicating a therapeutic role for EVI5 in inhibition of cancer metastasis."
32393392
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"SB431542 inhibited TGFbeta1 induced SMAD3 phosphorylation and abrogated the small basal expression of pSMAD3 seen in control cultures without exogenous TGFbeta, indicating autocrine activity (XREF_FIG), as the growth medium supplements contained minimal TGFbeta activity and did not account for the baseline activity (XREF_SUPPLEMENTARY)."
23284691
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"Exogenous TGF-beta1 induced temporally dependent alterations in Smad2 and Smad3 gene expression."
19374154
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"In addition, knockdown of eIF3a suppressed TGF-beta-induced HSC proliferation and the expression of alpha-smooth muscle actin (alpha-SMA) and collagen I. Furthermore, knockdown of eIF3a inhibited the expression of p-Smad3 induced by TGF-beta1 in HSCs."
27262813
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"As expected, TGF-beta1 addition to the culture of TKPT increased expression levels of p-Smad3."
32281211
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"A recent study reports that naringenin significantly inhibits the transcription of SMAD3 induced by TGF-beta1 and reduces the probability of TGF-beta1 binding to its receptor TbetaRII, thereby inhibiting receptor dimerization and downstream signaling transduction, inhibiting cell migration and invasion."
33842467
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"Finally, to determine if TGFbeta signaling pathway was activated in cervical tissue we evaluated the expression of Smad2 and Smad3 which are known to be activated by TGFbeta1."
19631925
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"Furthermore, TGF-beta1 induces Smad3 expression to regulate microRNAs and Smad ubiquitination regulatory factor (Smurf) to exert its pro fibrotic effect."
29518614
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"We used recombinant human TGF-beta1 (2ng/ml) to stimulate HDFs for 24h, which showed TGF-beta1 significantly induced the Smad3 mRNA expression and downregulated the IL-17RA and IL-17RC expression on HDFs (P < 0.01; Figure XREF_FIG B)."
28217129
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"Then, transfecting A549 cells with HBLV-h-STAT3 revealed that overexpression of STAT3 can significantly increase the expression of p-SMAD3 induced by TGF-beta1 (XREF_FIG)."
33760151
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"The addition of TGF-beta2 (2 ng/mL), but not TGF-beta1, restored the levels of p-ERK1/2, p-Smad3, and alpha-SMA (XREF_FIG)."
20720562
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"Treatment of TGF-beta1 further increased the expressions of TGF-beta1 and Smad3, whereas the TGF-beta1 inhibitors group exhibited lower expressions of TGF-beta1 and Smad3 than those of the model and TGF-beta1 group (all P < 0.05)."
28110342
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"Moreover, TGF-beta1 supplement significantly restored Erk1/2 and SMAD3 phosphorylation levels individually or simultaneously which is inhibited by LAE supplement in three subtypes of breast cancer cells."
33602253
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"Confocal immunofluoresence assay with anti-pSmad3 antibody (used to detect levels of Smad3 when phosphorylated at Ser423/425) showed that TGF-beta1 treatment increased pSmad3 levels in VSMCs, especially in the nucleus; and pretreatment with 3AB or PJ34 prevented the TGF-beta1-induced increase (XREF_FIG)."
22073128
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"TGF-beta1 increased the invasive ability of JEG-3 cells and the expression levels of Smad2 and Smad3; hence, TGF-beta1 promotes the invasion of JEG-3 probably by activating Smad2 and Smad3."
28432277
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"Protein expressions of p-Smad3, PI3K, p-Akt and p-GSK-3beta were significantly up-regulated by stimulation of TGF-beta1."
31102891
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"TGFbeta1 promoted the expression of c-JUN, SMAD3 and p-SMAD3 and suppressed miR200a expression and SB431542 reversed these effects (XREF_FIG)."
33122398
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"The protein expression levels of p-Smad2 and p-Smad3, which are enhanced by TGF-beta1, were markedly decreased in Rg3 treated KFs (XREF_FIG)."
29328420
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"Similarly, our results showed that TGF-beta1 could increase the protein expression of p-Smad3 and TWIST1/2 in both HEC-1A and Ishikawa cells at 8 h. ISL is a flavonoid that showed anti-tumor effects in variety of cancer cells."
| PMC
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"We found that low concentrations of TGF-beta1 inhibited the expression of P-AKT and promoted the expression of P-Smad3, while high TGF-beta1 concentrations had the opposite effect (XREF_FIG - XREF_FIG)."
33495412
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"The strong elevation in Cox-2 expression following TNFalpha+ TGFbeta1 stimulation was almost totally abrogated by joint siRNA induced downregulation of p65 and Smad3 expression."
28553282
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"In cultured neonatal myocardial cells, different level TGF-beta1 could significantly increase the total protein, and TGF-beta1 (3 ng/ml) could increase the expression of mRNA and protein of Smad3 and continued for 8 h of cultured cardiomyocytes."
21158069
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"TGF-beta1 increased the expression of NFYA, TGFbetaR1, TGFbetaR2, p-Smad2, p-Smad3, p-Smad3L, and p-Akt (Ser473), and knocking down NFYA decreased the expression of these genes, except for TGFbetaR2."
28212548
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"We firstly examined the expression of Smad2 and Smad3 induced by TGF-beta 1 in normal NIH/3T3 cells."
18506886
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"Because TGF-beta1 can induce p-Smad3 expression, 17 we treated lung adenocarcinoma cells with different concentrations of TGF-beta1 (0-20ng/ml)."
28005074
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"Western blot analysis revealed a high level of p-Smad2 and p-Smad3 in response to a 1-hour treatment with TGF-beta1."
31496148
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"Levels of p-SMAD3 were increased less than two-fold by TGFbeta1 at 48 hours and not at all at 96 hours."
24632204
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"Western-blot assay also showed that TGF-beta1 treatment increased pSmad3 levels in whole and nuclear extracts; and knockdown of PARP1 by siRNA or inhibition of PARP activity by 3AB or PJ34 prevented TGF-beta1-induced pSmad3 increase in both whole and nuclear extracts (XREF_FIG)."
22073128
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"The levels of phospho-SMAD3, which were increased by TGF-beta1 treatment, were decreased by eupatolide treatment in a time dependent manner (XREF_FIG)."
29113242
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"TGF-beta1 significantly induced the expression of Smad3 mRNA and downregulated the IL-17RA and IL-17RC expression on HDFs."
28217129
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"We then co-treated CFs with Ex-527, TGF-β, and RES and found that Ex-527 offset the effect of RES on TGF-β1–induced increased Ac-Smad3 protein expression in CFs ( xref )."
34177571
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"Consistent with the reduction in phosphorylated Smad3 by ASTX, the nuclear translocation of Smad3 was also reduced by ASTX, whereas TGFbeta1 treatment increased nuclear Smad3 levels."
25450180
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"TGF-beta1 can upregulate the expression of Smad2 and Smad3 during tissue fibrosis, a process that is negatively regulated by Smad7 as part of a negative feedback loop."
33365066
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"Consistent with XREF_FIG, Losartan blocked the TGFbeta1 mediated increase in p-ERK but levels of p-SMAD3 were not modulated by TGFbeta1 or by the inhibitors (XREF_FIG)."
24632204
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"Western Blot (WB) analysis suggested that TGF-beta1 treatment could effectively increase the expression of alpha-SMA, COL1, Smad3, and p-Smad3, which could be inhibited by SIS3 treatment."
32447720
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"The results revealed that TGF-beta1 promotes Smad3 transcription."
25120713
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"In the present study, TGF-beta1 treatment was shown to increase the phosphorylation levels of Smad2 and Smad3, confirmed by the enhancement of the transcription and expression of collagen mRNA shown in XREF_FIG, XREF_FIG, XREF_FIG."
23418473
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"Regulation of TbetaR1, SMAD4, SMAD3, SMAD7 and TIMP-1 mRNA expression by TGF-beta1 in HTR-8 and SVneo cells."
25295107
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"Following transfection of A549 cells with miR-320a-3p mimic, the expression of p-SMAD3 induced by TGF-beta1 was significantly reduced, while total SMAD3 was unaffected (XREF_FIG)."
33760151
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"TGF-beta1 increased the expression of TGFbetaR1, p-p70S6K, p300, p-Smad3, p-Smad3L, PDGFRbeta, p-ERK, and alphaSMA in Lx-2 cells, and this activation was repressed by the addition of BCAA."
28212548
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"NOF-CAF cells ' expression of p-SMAD2 and p-SMAD3 in response to TGF-beta1 demonstrated intact TGF-beta signaling."
30087253
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"In choriocarcinoma cells, the expression of Smad3 could be promoted by TGF-beta1, and suppressed by p38 MAPK inhibitor [XREF_BIBR]."
27494897
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"TGF-beta1 increased the expression of type I collagen and Smad3 protein in d4 primary HSCs, while SA-B inhibited their expression."
14969849
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"TGF-beta1 promotes the expression of Smad3 and inhibits the expression of Smad7 during the activation of PSCs."
20943464
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"Correlation of Promoter Binding by SMAD3 and Altered Gene Expression by TGFbeta1."
21625455
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"The underlying mechanism might be the down-regulated expression of AT1R mRNA and Smad-3, increased production of Smad-7, and blocking effect of TSN IIA on TGF beta1 and Smads signal pathway in local myocardium."
19662366
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"Consistent with these reports, we found that TGF-beta1 could up-regulate the protein expression of TGF-beta RI, TGF-beta RII, p-Smad2 and p-Smad3."
28458155
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"TGFbeta1 induced alpha-SMA expression was reduced by MEK inhibition (U0126); however, the levels of pERK, pSmad3, or the extent of the interaction between pSmad3 and CBP induced by TGFbeta1 were not affected by FSK."
18936144
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"TGF-beta1 caused upregulation of smad3 and p-smad3 expressions, while decreased smad7 expression."
24864150
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"Both SFRP1 and FRZB inhibit the TGFbeta1 induced increase of active beta-catenin, but do not influence the TGFbeta1 induced phosphorylation levels of SMAD3, positioning Wnt signaling activity downstream of the active TGFbeta signal in lung fibroblasts, but not in alveolar epithelial cells."
25317206
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"For the expression of Smad3, the OGD group had significantly higher level of Smad3 than the control group, and transfection of TGF-beta1 or Smad3 further up-regulated the Smad3 expression (P < 0.05, XREF_FIG)."
28110342
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"Following transfection of A549 cells with miR-320a-3p mimic, the expression of p-SMAD3 induced by TGF-β1 was significantly reduced, while total SMAD3 was unaffected ( xref )."
33760151