IndraLab
Statements
sparser
"PTP1B knockdown enhanced sensitivity of T47D cells to prolactin phosphorylation of Stat5 by reducing the EC(50) from 7.2 nmol/L to 2.5 nmol/L. Immunohistochemical analyses of two independent clinical breast cancer materials revealed significant negative correlations between levels of active Stat5 and PTP1B, but not TC-PTP."
reach
"Interestingly, we have observed that the ECM features - stiffness and collagen-I ligand density - exert distinct effects on PRL initiated signals, using polyacrylamide hydrogels in vitro : elevated density reduces PRL phosphorylation of STAT5, whereas stiffness augments PRL signals to ERK1/2 [XREF_BIBR]."
reach
"Interestingly, prolactin treatment only induces a transient STAT5 phosphorylation and nuclear translocation when the mammary epithelial cells are isolated and cultured in 2D or in suspension, and the transient STAT5 activation fails to differentiate and turn on milk protein expression."
sparser
"We therefore examined the effect of ROS pretreatment on prolactin-stimulated STAT5 phosphorylation in the glucose-responsive, insulin-secreting pancreatic β-islet cell line βTC-6 [ xref ], As expected, brief stimulation of quiescent βTC-6 cells with 15 nM prolactin resulted in the phosphorylation of STAT5 ( xref )."
reach
"Prolactin stimulation of HEK293 cells that had been transfected with nonmutant His188, but not mutant Arg188, PRLR constructs induced STAT5 phosphorylation over a range of time periods and at physiologically relevant concentrations (XREF_FIG, and XREF_SUPPLEMENTARY and XREF_SUPPLEMENTARY and XREF_SUPPLEMENTARY)."
reach
"However, our results are the first to demonstrate that pSTAT5 immunoreactivity also appears in the medial part of the preoptic area following suckling, and its distribution is the same as after exogenous prolactin injection and in continuously lactating dams, which suggests that prolactin itself caused the phosphorylation of STAT5 following suckling."