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USP9X activates Hydrogen-Ion Concentration. 2 / 2
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"Quantitatively analyzing such single particle regions where both FAM and ATTO647N signal spots were present, and altering the pH of the imaging buffer the immobilized aC’ dots were immersed in (Figure S5), we found that the FAM/ATTO647N intensity ratio still increased with pH, albeit with large deviations across different spots."