IndraLab

Statements


TP53 increases the amount of TP53. 364 / 561
1 1 | 351 2 9

isi
"Pattern of nucleotide variants of TP53 and their correlation with the expression of p53 and its downstream proteins in a Sri Lankan cohort of breast and colorectal cancer patients."

reach
"Increasing the nuclear p53 levels depletes the pool of cytoplasmic p53, which is more effective in promoting apoptosis by acting on the mitochondria (62)."

reach
"Lack of p53 protein in MCF7 p53 cells was confirmed by immunoblot analysis after doxorubicin treatment to induce maximum p53 levels upon DNA damage activation (Fig. 1c)."

reach
"Ectopic expression of a temperature sensitive (ts) p53 allele induced expression of p53 target genes and elicited both G1 and G2/M cell cycle arrest upon shift to the permissive temperature."

reach
"These results suggest that a ' slipped mispairing ' mechanism occurring during DNA replication may generate p53 intragenic deletion in human esophageal cancer, leading to abolished p53 mRNA expression."

reach
"Blocking of potential Wrap53 and p53 RNA hybrids reduces p53 levels nearly as efficiently as Wrap53 knockdown, strongly suggesting that Wrap53 regulates p53 via Wrap53 and p53 RNA interaction."

reach
"The loaded DOX was control released under higher GSH situation in HeLa tumor cells, and the nanosystem showed enhanced p53 gene delivery inducing an increase in p53 protein expression in these cells."

reach
"Inactivation of tumor-suppressor gene p53 decreases expression of p53 target genes causing malignancy, and histone deacetylase (HDAC) inhibitors reactivate the expressions of the genes suppressing tumor growth."

reach
"Wolf D. & Rotter V. (1985) Major deletions in the gene encoding the p53 tumor antigen cause lack of p53 expression in HL-60 cells."

reach
"All alleles carried a transcriptional stop element flanked by LoxP sites (Lox-Stop-Lox or LSL) in the first p53 intron to allow regulatable p53 expression (XREF_FIG)."

reach
"Silencing of p53 or p73 by RNA interference significantly suppresses p53 transcription under physiologic conditions or in response to cellular stress."

reach
"wt p53 gene transfection upregulated the expression of p53 in Bel7402/5-FU cells."

reach
"Notably, an antisense transcript of p53 (wrap53) modulates p53 expression through targeting the 5 ' UTR of p53 [XREF_BIBR]."

reach
"Human maintenance DNA (cytosine-5)-methyltransferase and p53 modulate expression of p53 repressed promoters."

reach
"We further tested the role of p53 in KDM5C induced migration by knocking down p53 expression using siRNA."

reach
"Wolf D. & Rotter V. (1985) Major deletions in the gene encoding the p53 tumor antigen cause lack of p53 expression in HL-60 cells."

reach
"Ad-p53 plus OBP-301 induced high levels of p53 protein expression without p21 induction, resulting in apoptotic cell death documented by active caspase-3 expression with a cytometric bead array and an increased subdiploid apoptotic fraction of the cell cycle."

reach
"The results showed that Ad-p53 downregulated the mRNA levels of TGF-beta1 and alpha-SMA, and upregulated the mRNA expression of p53."

reach
"This stabilization of p53 was sufficient to induce expression of the p53 target p21 (XREF_FIG) Interestingly, unlike diploid cells, retinoblastoma cell lines exhibit a low level of detectable p53 even when not treated with damaging agents."

reach
"The low p53 signature HCC had increased expression of the p53 repressed cell cycle positive regulatory genes CCNB1/2, E2F2/3, and FOXM1."

reach
"While upregulation of p53 by Nutlin-3 apparently decreased the invasion and increased the expression of p53 in NCI-H929 (p < 0.05) but not U266 cells."

reach
"We also show that p53 mediated suppression of hTERT transcription requires a minimum threshold level of p53, and p73 abrogates p53 mediated suppression by reducing p53 levels through the activation of HDM2."

reach
"P53 itself was released from the E1B body by ectopic expression of WTX-NS and accumulated in the nucleus (XREF_FIG), contributing to the observed increased expression of p53 target genes."

reach
"Together, IR induced modifications of p53 and Mdm2 stabilize p53 and enhance transcription of p53 regulated proteins."

reach
"A p53 antisense gene, Wrap53, enhances p53 mRNA levels via the 5 ' UTR."

reach
"Indeed, apoptotic signal which was enhanced through p53 and p21 activation and knockdown of p53 expression also moderately affected the DS induced apoptosis."

reach
"Hence, we postulated that the loss of SOCS1 might result in efficient p53 activation and compensatory upregulation of p53 expression, and increase in the expression of MET and p21."

reach
"As expected, WT p53 increased p53 target gene expression (Cdnk1a, Mdm2) in each cell line."

reach
"Western analysis showed that both p53 and ISG20L1 protein levels were elevated after cisplatin treatment and this increase was primarily p53 dependent as the shRNA targeting p53 significantly decreased the cisplatin induced elevation in p53 and ISG20L1 protein levels."

reach
"Wolf D. & Rotter V. (1985) Major deletions in the gene encoding the p53 tumor antigen cause lack of p53 expression in HL-60 cells."

isi
"In addition, URM1 knockdown downregulated the expression of the apoptosis-related factors JNK1/2 and TP53 and upregulated the phosphorylation of JNK1/2 and P53."

reach
"Since most cervical tumors are infected with HPV, and the tumors originate through p53 gene mutation caused by the said interaction, which leads subsequently to the overexpression of p53 oncoprotein, lack of the latter in the remaining 11 cervical tumors may either be the result of technical shortcomings, or the tumor may arise in such circumstances through a p53 independent pathway."

reach
"Furthermore, we found that knockdown or knockout p53 could significantly inhibit the expression of p53, classical p53 target genes (p21 and PUMA) [XREF_BIBR, XREF_BIBR], and these three genes."

reach
"Also, p53 DNA binding sites (E1-Deiry et al., 1992) were not found even if 1 or 2 mismatches were allowed during the search, suggesting that murine and human p53 intron 4 modulate p53 expression by di[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"The p53 transcription inhibitor, pifithrin-alpha, (10 microM) significantly reduced the expression of p53 in microglia and significantly modulated the levels of microglial apoptosis induced by activation."

reach
"Further, all cells transduced with ncmDeltaNGFR-huARF were positive for p53 driven beta-gal activity (XREF_FIG) and expression of p53 activating molecules resulted in a lower overall proportion of transduced cells at 68h after transduction (XREF_FIG), indicating that the growth rate of cells transduced with p53 activating molecules was slowed."

reach
"When examined in the reconstituted ACF chromatin assembly coupled transcription system, Su-p53 fails to activate transcription from pWAFMLT chromatin, suggesting that sumoylation of p53 inhibits p53 dependent transcription."

reach
"It was not surprising that NR5A2 suppressed the growth of HNSCC cell lines with normal/higher p53 expressed by upregulating TP53 expression."

reach
"PI3K activity and the expression of pAkt-S473 increased in a time dependent manner, pmdm2-S166, p53 were also increased wortmannin inhibited phosphorylation of mdm2 and improved the p53 expression."

reach
"We found that the HSP90 inhibitors augmented endogenous wild-type p53 expression but rather down-regulated the p53 level induced by Ad-p53."

reach
"In control cells, blockage of p53 degradation by the proteasome inhibitor MG132 raised the level of p53; however, in BCCIPbeta kd cells, MG132 induced a smaller increase in p53, suggesting that less degradation of p53 was existed and thus blocked."

reach
"Consequently, NF-kappaB-dependent p53 activity induced the expression of p53 regulated genes PUMA and p21 (waf1) as well as apoptosis."

reach
"We found that Ad-p53 promotes apoptosis and inhibits HSC proliferation in a time- and dose dependent manner by modulating the expression of p53, transforming growth factor (TGF)-beta1 and alpha-SMA."

reach
"Recent research pointed out that deoxyelephantopin regulated the apoptosis and cell cycle arrest in HCT 116 and KB cells directly by p53 which enhanced the expressions of p21, Bax, and p53 XREF_BIBR."

reach
"We now show that p53 genetic suppression in MB cells with low basal p53 protein expression levels (D283 and UW228) significantly reduced drug responsiveness, suggesting opposing roles for low p53 protein expression levels."

reach
"However, the NF-kappaB sequence in the p53 promoter region is required for rosiglitazone to increase p53 transcription in this study [XREF_BIBR]."

reach
"JMN-1B cells treated with rapamycin showed dephosphorylation of p70S6 kinase, one of the targets of mTOR pathway, but rapamycin did not influence the p53 levels produced by Ad-p53."

reach
"Cells with an engineered deletion of the p53 CoTC site exhibit impaired p53 3'-end processing, decreased mRNA and protein levels of p53 and its transcriptional target p21, and altered cell cycle progression upon UV-induced DNA damage."

reach
"Consistent with the finding, western blot analysis showed that p53 silencing markedly reduced the expression of P-p53 and p21 (XREF_FIG)."

reach
"Wolf D. & Rotter V. (1985) Major deletions in the gene encoding the p53 tumor antigen cause lack of p53 expression in HL-60 cells."

reach
"Furthermore, efficient stable knockdown of p53 in TPC-1 (TPC-1 sh-p53) cells effectively abrogated expression of p53, p21, MDM2, and PUMA in response to APG115 treatment, whereas stably transfected negative control shRNAi TPC-1 (TPC-1 sh-NC) cells did not cause concentration dependent upregulation of those proteins."

reach
"It is plausible that the p53 and Che-1 proteins, both targets of the same DNA damage checkpoint kinases, cooperate to induce expression of p53 and p53-target genes."

reach
"To further investigate the role of p53 in 5-Fu co-treated with Gyp induced apoptosis, pifithrin-alpha, an inhibitor of p53, was added to diminish the expression of p53, and then intracellular ROS level, DNA damage and cell viability were detected."

reach
"P53 stability and transcriptional activity is modulated by PER2 in unstressed cells by blocking Mdm2-dependent ubiquitination and transcription of p53 target genes (68)."

reach
"While our previous work has demonstrated that p68 stimulates expression of p53 target genes that are normally induced by activated p53, we reasoned that, in a different transcriptional context (i.e. the p53 intron 4 promoter), p68 might act to repress p53 transcriptional activity, having previously shown that p68 can, in some contexts, repress transcription."

reach
"Wolf D. & Rotter V. (1985) Major deletions in the gene encoding the p53 tumour antigen cause lack of p53 expression in HL60 cells."

reach
"The expression of p53 was decreased by p53 siRNA, which inhibited the expression of Sox9 and Type II collagen."

reach
"In addition, using DNAJA1-knockdown HN31 cells by a DNAJA1-shRNA and DNAJA1-knockout HN31 cells, generated by another CRISPR-Cas9 system used in Supplementary Fig. S2B, C, we confirmed that overexpression of DNAJA1 or mutp53 rescued mutp53 levels and migratory potential of DNAJA1-depleted HN31 cells (Supplementary Fig. S3A, B)."

reach
"In Human Arterial Smooth Muscle Cells, it has been demonstrated that Rg1 induces p53 transcription and increases p53 protein levels, thought to play a role in the inhibition of proliferation of such cells."

reach
"Notably, parkin is a p53 transcriptional repressor that interacts with the p53 promoter and reduces p53 expression and activity [XREF_BIBR]."

reach
"Simultaneous Ad-ARF and Ad-p53 infection increased p53 protein levels not only in a wild-type p53 expressing cell line, but also in cell lines with deleted p53."

reach
"Because p53 level is increased by radiation in p53 expressing cells, it is possible that the presence of p53 may be the determinant for the radiosensitizing effect of 2DG."

reach
"The expression of the p53 target TP53 inducible glycolysis and apoptosis regulator (tigar, ISCW020485), which was recently shown to inhibit glycolysis, was down-regulated in tick nymphs, midguts, salivary glands, and ISE6 cells."

reach
"Furthermore, HBP1 knockdown impaired expression of endogenous p53 (XREF_FIG), suggesting that HBP1 was able to activate transcription of p53."

reach
"Increased functionality of p53 in podocytes silenced for VDR was further confirmed by display of enhanced transcription of both Agt and renin by approximately two fold.SIRT1 is a deacetylase and modul[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Most studies followed with interest the p53 based apoptosis pathways (Stoyanova et al., 2008, 2009a) and revealed that a positive feedback loop between DDB2 and p53, where p53 activated DDB2 expressio[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"We conclude that (i) miR-125b and miR-150 target p53, which in turn regulates RelA and NFkB and miR-146a expressions; (ii) reduced miR-125b and miR-150 expressions, increased p53 level and decreased RelA and NFkB and miR-146a expressions originate from mutant HTT (iii) p53 directly or indirectly regulates the expression of miR-146a."

reach
"SNVs of TP53 were commonly observed in TNBC and increased TP53 mRNA expression."

reach
"The overexpression of P53 protein has not been induced by P53 gene abnormalities and MDM2 protein synthesis was independent from respective gene amplification."

reach
"By direct binding, JNK also targets p53 for ubiquitin mediated degradation involving Mdm2-p53 degradation pathway Therefore, inactivation of JNK by anti-sense JNK1 or SP600125 would decrease the amount of JNK-p53 and/or Mdm2 and p53 complex to increase the steady state level of p53 by preventing p53 degradation in non stressed cells."

reach
"The stabilized wild-type p53 after 24 h of 10 muM treatment with MC robustly activated transcription of the p53 target genes mdm2 and p21."

reach
"We found that inhibition of wild type p53 led to a 3.2 fold increase in PAPP-A levels (XREF_FIG) suggesting that wild type p53 represses the transcription of PAPP-A."

reach
"Here, we report a novel long noncoding RNA, p53 upregulated regulator of p53 levels (PURPL), functions as an oncogene to promote cell proliferation, colony formation, migration, invasiveness, and inhibits cell death in melanoma cells."

reach
"Thus, PIDD, a target gene product of p53, can increase p53 protein levels in a positive feedback mode."

reach
"A similar assessment of TP53 using RNA also showed equal expression of mRNA for endogenous TP53 (TP53 3ʹ UTR) and increased total TP53 expression (TP53 total; revised Supplementary Fig. 3D, E, left)."

reach
"When a cell incurs DNA damage by genotoxic, chemotoxic stress, or receives aberrant signals from oncogene activation, p53 is activated causing an elevated level of p53 associated with its acetylation and phosphorylation [XREF_BIBR - XREF_BIBR]."

reach
"Steady-state levels of both p53 and Wrap53 mRNAs increase upon DNA damage, indicating that Wrap53 not only maintains basal p53 mRNA levels but also plays a role in stabilizing p53 mRNA in response to [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Under conditions of stress, posttranscriptional modification of p53 leads to its dissociation from Mdm2 and translocates to nucleus to cause transcription of p53 dependent proapoptotic genes [39]."

reach
"These results demonstrate that the p53 mRNA NPs effectively restored p53 expression in vivo and significantly enhanced the anti-tumor effects of aPD1 therapy in HCC growing outside the liver.Using the same model, we also harvested tumors and lymph nodes to examine the number and phenotype of immune cells and the changes in secreted cytokines after four cycles of treatment."

reach
"Thus, restoration of wildtype p53 expression in HPV-positive cancer cells induces the expression of p53 transcriptional targets leading to cell cycle arrest, apoptosis and senescence (5–8)."

reach
"Remarkably, treatments with UV radiation, CDK inhibiting (flavopiridol, DRB, roscovitine), transcription inhibiting (actinomycin D), and p53 inducing compounds (doxorubicin, etoposide, nutlin-3) not only increase p53 levels [XREF_BIBR, XREF_BIBR, XREF_BIBR], but also enhance the protein protein interactions between HEXIM1 and p53 (XREF_FIG) [XREF_BIBR]."

reach
"sgTrp53-(2) induced nearly complete depletion of p53 protein, while sgTrp53-(1) induced expression of aberrant p53 protein that migrated faster than wild-type p53 protein in MLL-AF9 cells (Fig. 1c)."

No evidence text available

reach
"Additional confounding factors include the fact that not all p53 gene mutations lead to increased p53 protein levels intracellularly [4] and not all tumors with increased intracellular p53 levels nece[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Although the expression levels of ICE1 and Hsp90 were not affected by UV irradiation and/or p53 knockdown, the expression level of p53 was increased by UV irradiation and was decreased by p53 knockdow[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

sparser
"To further investigate the role of RNF20 and RNF40 in p53 stabilization and transcription of p53 target genes, p53 protein expression levels were analyzed using western blot analysis, and the relative mRNA expression levels of p53, p21, PUMA, mash1 and Oct4 genes were determined using RT-qPCR in RNF20- or RNF40-knockout cells."

reach
"CD24 competitively inhibits ARF binding to NPM, resulting in decreased ARF, increase MDM2 and decrease levels of p53 and the p53 target p21 and CDKN1A."

reach
"This suggestion is supported by our finding that treatment of mice with the USP7 inhibitor P5091 effectively eliminated SnCs and reduced the expression of SASP factors caused by DOX.In conclusion, we reveal that USP7 is a novel senolytic target, and USP7 inhibition can selectively kill some SnCs with p53 downregulation in vitro and clear SnCs induced by chemotherapy in mice in part by destabilizing MDM2 to increase p53 expression."

reach
"This data shows that transfection of an siRNA targeting p53 reduces p53 levels in DLD-1 cells prior to adriamycin treatment, as well as limiting the ability of these cells to fully recover p53 levels as a function of increasing adriamycin concentrations, despite the fact that the increase in p53 levels following DNA damage occurs through post-translational stabilization of p53 protein."

reach
"Geminin depletion induces DNA re-replication in cancer cells, regardless of the presence or absence of the tumor suppressor protein p53, but p53 +/+ cancer cells induce expression and phosphorylation of p53, as well as expression of the CDK specific inhibitor p21 whose transcription is p53 dependent [; XREF_FIG]."

reach
"Given the opposing responses of MDM2 and p53 elicited by HB-EGF (Fig. 3A), the enhanced association of p53 and MDM2 by HB-EGF stimulation (Fig. 3B) supported the hypothesis that MDM2 may negatively regulate p53 expression, possibly by stimulating direct binding of MDM2 to p53 following HB-EGF treatment [33]–[35]."

reach
"Taken together, the evidence suggests that CTCE-targeted NPs demonstrated significantly enhanced cellular uptake, mRNA transfection efficiency, and intratumoral accumulation compared to non-targeted NPs irrespective of tumor site/stroma, supporting the use of CTCE peptide ligands for selective HCC cell targeting.To determine whether the targeted p53-mRNA NPs could induce the expression of therapeutic p53 in p53-null RIL-175 cells, we first checked p53 protein expression after treatment with CTCE-p53 NPs versus SCP-p53 NPs."

reach
"Moreover, silencing of p53 effectively down-regulated the expression levels of total p53, Ser 15-p53 and PIGs, and suppressed caspases activation, which suggest that cell apoptosis induced by DSeA and TRAIL is p53 dependent."

reach
"Axolotl p53 can activate the transcription of human p53 target promoters in human cells."

reach
"The results showed that Ad-p53 and GM-CSF could mediate high-level expressions of p53 and GM-CSF in Hep-2 cells.Ad-p53/GM-CSF and Ad-p53 carried the same p53 gene expression cassette and the expressio[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Previous studies have demonstrated that chemical activation of p53 in PEL cells is sufficient to induce the expression of p53 target genes and lead to cell growth inhibition and apoptosis [XREF_BIBR]."

reach
"Curcumin can activate the signaling pathway of p53, and increase the expression of p53, and enhance the binding activity of p53 DNA, which upregulates the expression of the p53, downstream effector Bax and thus induces tumor cell apoptosis [XREF_BIBR]."

reach
"The PURPL gene (p53 upregulated regulator of p53 levels), an intergenic long non coding RNA (lncRNA), known to regulate basal p53 levels was also found to be upregulated in the ' responders ' (FC = 0.044)."
| PMC

reach
"These findings provide evidence that p53 (-/-) mice and WT mice grafted with p53 (-/-) bone marrow cells are more prone to developing elastase induced emphysema, supporting a protective role of p53, and more precisely p53 expressed in macrophages, against emphysema development."

reach
"Re-folding of this mutated and accumulated p53 leads to restoration and activation of defective proteins, resulting in high levels of active p53, which then induces apoptosis [XREF_BIBR]."

reach
"Although there was no significant difference in expression of CDKN1A, VDR, and TP53INP1 between CD24 + PC3 cells and CD24 - PC3 cells, the transfections of WT TP53, mutants TP53 R273H, TP53 V143A, and TP53 R280T, but not mutant TP53 R175H, induced the expression of these p53 target genes in CD24 - PC3 cells but not in CD24 + PC3 cells (XREF_FIG)."

reach
"Therefore, MDM2 is a p53 target which in turn serves to limit the amount of p53 via its efficient targeting degradation."

reach
"The results were consistent with RT-qPCR analysis, indicating that the exogenous p53 gene delivery could enhance the expression of wild-type p53 gene in tumor cells, and further achieve the p53 based gene therapy."

reach
"In congruence we find that inhibition of p53 degradation with MG132 under isotonic conditions increases p53 expression in NIH3T3 fibroblasts."

reach
"The p53-induced long noncoding RNA (lncRNA) lincRNA-p21 is proposed to act in cis to promote p53-dependent expression of the neighboring cell cycle gene, Cdkn1a/p21."

reach
"The authors employed a p53-inducible system where the addition of doxycycline triggered the expression of p53 wt (H1299-Tet-On-p53) or mutant p53R273H (H1299-Tet-On-p53-273H)."

reach
"As expected siRNA mediated knockdown of p53 effectively decreased or abolished p53 protein expression below detection in all examined cell lines (XREF_FIG)."

reach
"Data analysis showed that Ad-p53 upregulated the mRNA expression of p53 and downregulated the mRNA expression of TGF-beta1 and alpha-SMA, compared with the normal saline group, and the differences were statistically significant (P < 0.05) (XREF_FIG)."

reach
"Transfection of Adv-p53 into U251 cells, as well as into U-373MG cells, induced the expression of exogenous wild type p53 (XREF_FIG, lanes 2, 4, 6, 8), the size of which is larger than that of endogenous mutated p53 (XREF_FIG, lanes 1, 3, 5, 7)."

reach
"Taken together, these findings suggest that Ad-p53 promotes apoptosis and inhibits the proliferation of HSCs in a time- and dose dependent manner by modulating the expression of p53, TGF-beta1 and alpha-SMA."

reach
"Here p53 expression was rescued in the p53 null epithelial carcinoma line H1299 by activation of a tetracycline (tet)-regulated p53 allele."

reach
"Ectopic expression of p53 or DNA damage induced expression of p53 can suppress the expression of Aurora A. Mechanistic studies show that p53 is a negative regulator for Aurora A expression through both transcriptional and posttranslational regulation."

reach
"In parallel, 3 '-end processing of TP53 mRNA is up-regulated to increase expression of TP53 (also known as p53) 133."

isi
"p63 expression is associated with high histological grade, aberrant p53 expression and TP53 mutation in HER2-positive breast carcinoma."

reach
"Ad p53 was used to induce transient overexpression of wild-type p53."

reach
"Inversely, ZNF668 depletion decreased both the basal p53 level and the stress induced p53 level."

reach
"Analysis of p53 in PHA stimulated PBLs revealed expression of p53 by non tumoral activated lymphocytes."

reach
"This may be explained by the elevation in the p53 protein level caused by IFN signaling dependent p53 stabilization (XREF_FIG and XREF_FIG)."

reach
"The miR-125b was first reported to reduce p53 protein levels and impair p53 induced apoptosis in human cells and the brain of zebrafish [XREF_BIBR]."

reach
"These findings suggest that the p53β/γ isoforms may induce transcription of p53 target genes in both a p53α-dependent and independent manner."

reach
"shRNA mediated knockdown of p53 in Sk-Mel-2 cells (Sk-Mel-2 shRNA (p53)) reduced p53 protein levels (XREF_SUPPLEMENTARY) and impaired Plk1i mediated reduction of cell viability (XREF_FIG)."

reach
"Furthermore, overexpression of the wild-type p53, mediated by transfection of p53 coding cDNA, induced cells to undergo apoptosis or cell differentiation."

reach
"In addition, overexpression of miR-300 significantly reduced p53 and apaf1 and/or IR induced p53 and apaf1 protein expression levels."

reach
"In addition, knockdown of p53 with siRNA diminished H O -induced high expression of p53, progerin, Lamin A/C and p21; meanwhile, knockdown of progerin with siRNA decreased H O -induced elevated expression of Lamin A/C, except p21 (Fig. 5b, d)."

reach
"Mutations in ihc p53 gene increases tlte level of p53 expression in ihe cell and create new MHC I restricied epitopes for T cell recogniUoa Furtliemiore, ihc upregulation of wild type p53 epitopes might exceed the level of expression required for activation of autoreactive tumor specific T cells.We selected peptides derived from the p53 sequence and tested ihclr binding to HLA-A2.1 molecules, using a biodiemical assay."
| PMC

reach
"Since the p53 related transcription factors p63 and p73 can modulate p53 functions and activate the transcription of some p53 target genes XREF_BIBR, XREF_BIBR, we examined the impact at the FLT1 promoter alleles using gene reporter assays and ectopic expression of p53 or of selected p63 and p73 isoforms."

reach
"Here, we report the suppression of basal p53 levels by a nuclear, p53 regulated long noncoding RNA that we termed PURPL (p53 upregulated regulator of p53 levels)."

reach
"The induced SMG7-p53 interaction is not a result of p53 stabilization, as the MDM2 inhibitor Nutlin increased p53 levels but had no effect on SMG7 binding (Supplementary Fig. S1b) ."

isi
"In addition, a subset of these samples were analysed for the protein expression of p53 and comparison made with the mutational status of TP53."

reach
"Further, p53 upregulation can modulate the expression of PUMA, a BCL2 family protein and an important mediator of p53 dependent apoptosis XREF_BIBR, XREF_BIBR."

reach
"PPMD1, a member of the PP2C family of Ser/Thr protein phosphatases that is induced in a p53 dependent manner in response to various environmental stresses, reduces the phosphorylation of p53 through negative regulation of p38 MAP kinase, and in turn suppresses p53 mediated transcription and apoptosis."

reach
"In another study, apoptosis of HeLa cells was induced by the upregulation of p53, which subsequently increased the expression levels of p53 and Bax."

reach
"TP53 silencing inhibited the expression of p53 and p21 either in control and treated cells."

reach
"Zhao et al. used RNA immunoprecipitation sequencing (RIP-seq) to demonstrate that EZH2 binds to the 5′ UTR of TP53 mRNA, which enhances transcription and translation of p53 protein."

reach
"Nonetheless, we postulated based on the requirement of SOCS1 to mediate p53 dependent cellular senescence [XREF_BIBR] that SOCS1 deficiency might increase p53 expression, similarly to inactivating p53 mutations."

reach
"The same effect is also achieved by ubiquitylases and deubiquitylases that fine-tune p53 turnover and miRNAs that modulate p53 levels, adding precision to this entire scheme."

reach
"Finally, a p53 agonist, 20 nM Nutlin 3, was revealed to increase the protein expression levels of p53, Bax and p21, and suppress cyclin E protein expression in the p53 + anti-miR-494 group compared with in the anti-miR-494 group (XREF_FIG)."

reach
"P53 translation negative regulators including miR-125b, miR-25 and nucleolin inhibit p53 expression and positive regulators including HuR and heterogeneous nuclear ribonucleoprotein (hnRNP) Q enhance p53 expression and p53 mediated apoptosis and cell cycle arrest [XREF_BIBR, XREF_BIBR - XREF_BIBR]."

reach
"In contrast, p53 25,26,53,54 combined with wild-type p53 drove elevated expression of select p53 target genes, including Noxa and Pidd, but not p21 and Mdm2, relative to levels in p53 +/- and p53 +/+ MEFs, suggesting that p53 25,26,53,54 activates wild-type p53 to induce expression of specific p53 target genes (XREF_FIG, XREF_FIG)."

reach
"Stabilization of p53 in hypoxia by HIF-1alpha increases the expression of p53 target genes, such as p21, and results in the initiation of apoptosis."

reach
"Continuous exposure of the pMAMneo/wt-p53 transfectants to 1 muM, dexamethasone for more than 24 h caused overexpression of wt-p53 followed by cell death with morphological changes typical of apoptosis."

reach
"In vitro and xenograft NSCLC models have shown that sequential therapy of cisplatin and p53 gene therapy enhances p53 expression."

reach
"Although activation of p53 by DNA damage induced p53 target expression in astrocytes, it did not alter hypoxia induced HIF-1alpha target expression."

reach
"These data suggest that although upstream regulators of p53 are functioning to induce p53 expression and phosphorylation in the mutant cell lines, the mutant p53 is not capable of inducing transcriptional activation of its targets."

reach
"qRT-PCR indicated that TP53 expression was reduced by > 80% for each p53-deficient cell line (Fig. 1a)."

reach
"We can state however that a good place to start would be to assess whether endogenous TP53 activates after various stress events and induces the transcription of specific TP53 targets."

reach
"As with RA, BMP4 treatment also transiently stabilized p53 protein, which activated expression of p53 target CDKN1A."

reach
"Expression of Egr1 and p53 in human carotid plaques and apoptosis induced by 7-oxysterol or p53."

reach
"Activation of p53 in MM by an Mdm2 antagonist induced p53 target gene expression and apoptotic cell death [XREF_BIBR]."

reach
"15 Here, we add a new mechanism by which Che-1 controls p53 mediated response : Che-1 and p53 interact and this interaction is involved in the regulation of p53 target genes selectivity, directing p53 transcription towards growth arrest genes."

reach
"Transfection of p53 in MPNST cells induced expression of miR-34a and other p53 dependent miRNAs."

reach
"Taken together, the key findings obtained from the present study elucidate that injection of p53 gene and UCA microbubbles guided by ultrasound could increase the expression of p53, thus inhibiting the tumor growth in rats with BC."

reach
"Overexpression of p53 can be induced by p53 gene mutations and other DNA damaging agents, such as ultraviolet (UV) light and ionizing radiation."

reach
"Quantitative analysis of p53 mRNA in PCBP2-downregulated cells revealed a lower level of p53 mRNA under normal conditions suggesting the involvement of PCBP2 in p53 mRNA stabilisation."
| PMC

reach
"In addition, the transfection of a plasmid DNA encoding wt-p53 to increase p53 expression decreased the viability of N2a cells and increased leakage of LDH in co-treated N2a cells compared with cells that underwent OGD/R and transfection with miR-29b mimics."

reach
"In gastric cancer cells, although fisetin increased p53 protein expression, inhibitor of p53 did not reduce fisetin-mediated apoptosis [3]."

reach
"Although knockdown of p53 diminished the C1 induced p53 expression, the increase of G2/M fraction after C1 treatment was observed in p53-siRNA trasfected A549 cells."

sparser
"Piperine was found to inhibit cell proliferation of triple negative breast cancer deficient in p53 (TNBC), estrogen receptor positive breast cancer cell, p53 deficient and estrogen receptor positive that express p53 ( xref )."

reach
"These data showed that both pathways did not influence the p53 expression induced by Ad-p53."

reach
"A protein protein interaction was detected with tetratricopeptide domain 5 (TTC5), which is recognized as a stress inducible transcription p53 cofactor that binds directly with histone acetyltransferase EP300 (p300) to augment p53 expression, stability, and/or activity XREF_BIBR."

reach
"Reconstitution of USP10 in CAKI-1 and CAKI-2 (WT p53 cell lines) restored p53 expression and increased p21 and BAX expression (XREF_SUPPLEMENTARY)."

reach
"An absence of p53 induction upon oxidative stress would further imbalance the cells toward cell cycle exit, with a lesser amount of NuMA preventing p53 targeted transcription of p21."

reach
"Although these studies illustrate the importance of lncRNAs in the p53 network as well as the functional heterogeneity of p53 regulated lncRNAs, the function of the vast majority of p53 regulated lncR[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Expression of Pirh2 decreases the level of p53 protein and abrogation of endogenous Pirh2 expression increases the level of p53."

reach
"Owing to that BA can induce transcription of p53 and activation of p53 target gene, p53 may be activated and involved in regulating CRC apoptosis in the early stage of BA treatment."

reach
"Senescence can be driven by Rb or p53 pathways, which are closely intertwined : Rb can block p53 destabilizing protein MDM2, thus increase p53 levels, promoting the expression of p53 targets, p21, Bax and PIG3 and cause senescence XREF_BIBR, XREF_BIBR."

reach
"Our results suggest that p53 may block oncogenic transformation by decreasing BCL6 stability via caspase-1 up-regulation, whereas aberrant BCL6 expression inactivates transactivation of p53 target genes, either by inhibiting p53 acetylation by p300 or repressing TP53 gene transcription."

reach
"The use of the selective p53 activators Tenovin-1 and NSC207895 could increase the protein expression of p53 as well as its phosphorylation, indicating activation of the p53 pathway [XREF_BIBR]."

reach
"Mutations in the tumor suppressor gene TP53 which can lead to an altered p53 protein expression are rare at time of diagnosis of ALL, yet occur more frequent at relapse indicating a more aggressive disease."

reach
"In H9C2 cells, we observed that Tyr treatment for 72 hr induced the accumulation of cytosolic p53 levels and increased p53‐parkin interaction (Figure 5a,b)."

reach
"Delivery of the wild type p53 gene to a cancer cell via a modified adenoviral vector induces expression of wild-type p53 protein and triggers growth arrest or apoptosis, causing tumor growth inhibition."

reach
"Finally, we showed that SNF5 knockdown inhibits p53 translation by eIF4E and replacement of eIF4E in SNF5 knockdown cells restores p53 expression and cell survival."

reach
"Transfection of Adv-p53 into U-87MG at MOI 1000 and into U251 cells at MOI 100 induced similar levels of expression of exogenous p53."

No evidence text available

reach
"Overexpression of p53 can be caused by both increased production of wild-type p53 and by production of mutant p53."

reach
"Moreover, activated p73 in the absence of functional p53 activates the transcription of p53 target genes such as p21 or Bax, and induces apoptosis in p53-null SAOS2 cells."

reach
"The transient knockdown of p53 abrogated the expression of p53 transcriptional target genes following MDM2 inhibition (XREF_FIG)."

reach
"COL4A1 is a major antiangiogenic gene induced by p53 in human adenocarcinoma cells, and p53 directly activates the transcription of the COL4A1 gene by binding to an enhancer region 26kbp downstream of its 3 ' end [XREF_BIBR]."

reach
"In particular, climacostol activated p53 inducing the upregulation of p53 protein levels in the nuclei through effects on p53 stability at translational level, as for instance the phosphorylation at Ser15 site."

reach
"Phosphorylation of p53 by TAF1 inactivates p53 dependent transcription in the DNA damage response."

reach
"Furthermore, HBP1 knockdown impaired expression of endogenous p53 (XREF_FIG D), suggesting that HBP1 was able to activate transcription of p53."

reach
"45 Activation of p53 in response to DNA damage and other forms of cell stress is generally mediated by inhibition of p53 degradation, leading to increased intracellular p53 levels."

reach
"Ubiquitination of p53 is mediated by E3 ubiquitin ligase (Cubillos-Rojas et al., 2017), ubiquitination and degradation of p53 disrupt p53-dependent transcription, and affect p53-promoted cell growth inhibition, G1 block and apoptosis (Sun et al., 2009; Sun et al., 2011)."

reach
"In this study, we found that OPD significantly up-regulated the expression of p53 by the accumulation of p53 and increased its stability in SK-Hep-1 cells."

reach
"The results indicated that, although cells overexpressing p53β significantly enhanced the luciferase activity for all three promoters, those that overexpressed p53γ showed only a modest increase for p21 and GADD45A promoters (Fig. S7), suggesting that, in the absence of p53α, overexpression of p53γ alone is not sufficient to increase the expression of these p53 transcriptional targets."

reach
"Notably, quantitative real-time RT-PCR revealed that the transcription of two known p53 target genes involved in metabolic regulation, GLS2 and TIGAR, was fully induced by doxorubicin in p53 3KR/3KR cells (XREF_FIG)."

reach
"Wolf D., Rotten V., Major deletions in the gene encoding the p53 tumor antigen cause lack of p53 expression in HL-60 cells, Proc."

reach
"After 1 h of viral adsorption, cells were washed and incubated with fresh medium for 24 h. Western blot analysis and RT-qPCR of p53 show that BKPyV infection dramatically increases the p53 expression at both protein and mRNA levels (Figure 1(a))."

isi
"In addition, a subset of these samples were analysed for the protein expression of p53 and comparison made with the mutational status of TP53."

reach
"Additionally, p53 can initiate apoptosis by inducing the expression of p53 upregulated modulator of apoptosis (PUMA), which leads to the release of cytochrome c from mitochondria and apoptotic cell death 48."

reach
"P53 functions primarily as a transcription factor and induces the expression of several p53 target genes such as p21 and PUMA (p53 up-regulated modulator of apoptosis)."

reach
"Complexes of p53 and CBP and p300 recognize specific promoter sequences to initiate the transcription of p53 targeted genes."

reach
"Clearly, the p53 mediated cell cycle arrest requires p53 dependent transcription and one or more of its downstream genes."

reach
"p73 or p53 directly regulates human p53 transcription to maintain cell cycle checkpoints."

reach
"XREF_BIBR - XREF_BIBR Cell penetrating beta-peptides that mimic p53 activation domain were shown to upregulate cellular levels of p53, p21 and HDM2."

reach
"LINC01021 (ENST00000512067) is a long intergenic non protein coding RNA (lincRNA), also known as p53 upregulated regulator of p53 levels (PURPL)."

reach
"Notably, disruption of RREB-1-mediated p53 transcription suppresses the expression of the p53 target genes."

reach
"Abrogation of p53 expression by p53 small interfering RNA significantly attenuated 6-DG-induced DR5 expression, thus rendering these cells resistant to TRAIL induced apoptosis."

reach
"Blocking p53 signaling in axolotls using the p53 inhibitor, pifithrin-alpha, inhibited limb regeneration and the expression of p53 target genes such as Mdm2 and Gadd45, suggesting a link between tumor suppression and regeneration."

reach
"There has been extensive work on several DNA inducible genes as human MM biomarkers of exposure to these agents, including p53 induction of DNA strand breaks, p53 expression, and apoptosis in cell lines, particularly in cultured mesothelial cells XREF_BIBR."

isi
"Pattern of nucleotide variants of TP53 and their correlation with the expression of p53 and its downstream proteins in a Sri Lankan cohort of breast and colorectal cancer patients."

reach
"For example, the p53 target protein Wig-1 (ZMAT3) has been shown to increase p53 levels by enhancing p53 mRNA stability, while 14-3-3 sigma inhibits Mdm2 mediated ubiquitination of p53."

reach
"Therefore, at least in tumor cells with wild type p53 elevation of cellular p53 levels could be of high therapeutic effect."

isi
"All PCCs harbored KRAS and TP53 mutations, and expressed cytokeratin 19, ki-67, and p53, while the expression of EpCAM and vimentin was variable."

reach
"In human hepatic carcinoma cell lines, UNC0379 reduced level of an inactive form of p53 (p53 K382me1), which correlated with SET8 downregulation, and induced normal p53 expression."

reach
"Therefore, p53-independent regulation of pro-fibrotic miR34a could contribute to the low expression of miR34a together with the high expression of p53 in our sample."

reach
"Interestingly, the mere presence of GC7 per se was not able to counteract the stabilization of p53 induced by MG132 (lane 6), suggesting that UV-stress increased the levels of p53 protein also through mechanisms other than its stabilization."

reach
"Finally, we determined if RPS27L silencing would reduce p53 levels induced by these p53 activating signals."

reach
"These different TP53 statuses allowed us not to test constitutive or non constitutive p53 expression without overexpressing abnormal TP53 in the TP53 neg cell lines, as is observed in the cells of patients."

reach
"Conversely, while TP53 knockdown decreased p53 levels, it did not affect p65 levels."

reach
"P53 protein levels are strictly regulated and are suppressed by a negative regulator of p53, the E3 ubiquitin protein ligase MDM2."

reach
"As shown in XREF_FIG, the expression of p53 and some of its target genes was markedly induced by PHLDB3 shRNA in HCT116 p53 +/+ cells, but not in HCT116 p53-/- cells."

reach
"MDM2 bound p53 inhibits p53 mediated transcription by preventing p53 from contacting transcriptional coactivators such as p300 and CBP and subsequently allows for p53 export from the nucleus to the cytoplasm via the ubiquitination proteasome system XREF_BIBR."

reach
"Background:. A transversion missense polymorphism of the TP53 tumor suppressor gene at the codon 72 codes proline instead of arginine causes an altered p53 protein expression and has been found to be associated with an elevated risk of various cancer; especially breast and lung cancer."

reach
"The lead compounds were able to stabilize not only the active conformation of wild-type p53 but also the mutant p53 forms, inducing the transcription of p53 target genes."

reach
"Of the onco related genes, wild type p53 stimulates expression of TSP-1 [32], and p53 alteration also affects the expression of TSP-1 in bladder cancer [11]."

reach
"Up-regulation of the endogenous p53 with benzo [alpha] pyrene -- a well-known p53 activator -- increased the expression of the p53 responsive positive control and the CYP2A6-5 '-Luc construct containing the intact p53 binding site but not the mutated CYP2A6-5 '-Luc construct."

reach
"Cancers commonly harbor point mutations in TP53 that cause overexpression of functionally inactive p53 proteins."

reach
"With the exciting findings of the reciprocal regulation of p53 by RBPs, such as the RBM38/HuR/MDM2/p53 regulatory loop, our knowledge of the complex regulation of p53 and the p53 network allows for the potential intervention with therapeutic approaches to upregulate the expression of p53, or p53 targets like p21."

reach
"For instance, genotoxic stress induced alphaB-crystallin by a p53 dependent mechanism whereby the p53 family inhibitor DeltaNp73, activated alphaB-crystallin gene expression."

reach
"This report suggests that the post-translational modification of p53 is not needed to increase p53 levels in the cells treated by the alkylating agents."

reach
"The p53-DN peptide stabilizes the WT p53 protein, causing a clear increase in p53 protein level, but suppresses its activation of downstream targets (e.g., p21)."

reach
"For example, Wrap53, a natural antisense transcript of the tumor suppressor gene p53, increased endogenous p53 mRNA levels by targeting the 5 ' untranslated region of p53 mRNA 78."

reach
"In Skp2B overexpressing cells (XREF_FIG), native p53 is rapidly degraded by Mdm2 and the denatured p53 fraction contributes to the elevated level of total p53 observed in these cells."

reach
"The expression of both p53 and p16 senescence regulators was significantly increased by Ang II (p53 : 1.39 +/-0.17, p16 : 1.19 +/-0.10-fold vs. the control), and inhibited by fimasartan."

reach
"Endogenous axolotl p53 was activated following UV irradiation (100 j/m 2) or treatment with an alkylating agent as measured using serine 15 phosphorylation and the expression of the endogenous p53 target Gadd45."

reach
"The results showed that overexpression of p53 significantly elevated the protein level of p53 in A549 and PC-9 cells (Figure 4A) and led to the growth of A549, and PC-9 cells were inhibited (Figure 4B), but after interfering with siRNA of p53, the protein level of p53 was dramatically decreased (Figure 4A), and accordingly, the proliferation of A549 and PC-9 cells was promoted instead (Figure 4B)."

reach
"P53 protein is accumulated in stress conditions and activates transcription of multiple p53 targets that halt abnormal proliferation of cells that otherwise may become tumorigenic [XREF_BIBR, XREF_BIBR]."

reach
"The data herein clearly showed that G-Rh2 triggers p53 dependent Fas expression and consequent activation of caspase-8 and p53 independent caspase-9-mediated intrinsic pathway to cause cancer cell death."

reach
"GA-dependent inhibition of PI3K/AKT upregulated the expression of p53 and miR-34a, which is induced by p53 and inhibits PD-L1 expression."
| PMC

reach
"P53 Inhibitor Reduced p53 Protein Expression and Attenuated Thermal Hyperalgesia in CCI Mice."

reach
"Phosphorylation in the N-terminal domain of p53 inhibits binding of hdm2, and enhances transcription of p53 so that levels of the p53 protein rise and it is not degraded.Following DNA damage, p53 also[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Overexpress WT p53 in tumor cells increase p53 protein level and lead to cell growth arrest or apoptosis (Ramqvist et al., 1993; McIlwrath et al., 1994)."

reach
"However, a similar cancer resistance can be brought about when additional copies of wild-type p53 are available to increase the magnitude of p53 expression and activity only in response to appropriate[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"A role for p53 and p16 overexpression in the formation of some PPSC, and p53 overexpression in the generation of precursor tubal pathology was identified."

reach
"Microinjection of p53 mRNA into early cleavage stages effectively resulted in overexpression of p53 protein and interfered with normal development."

reach
"These findings suggest that p53γ might act in conjunction with p53α or other p53 isoforms in a promoter-specific manner to enhance the expression of p53 transcriptional targets."

reach
"Hence, these results demonstrate that infection of RGNNV induces p53-dependent G1 delay.To determine whether RGNNV infection induced p53-dependent G1 arrest by mediating p53 level and activity, we analyzed p53 levels after RGNNV infection in GS cells by immunofluorescence staining and Western blot analysis."

reach
"In addition, p53 dependent induction of MDM2, another p53 target which acts in a feedback loop to negatively regulate levels of p53 3334 was decreased in MIF treated cells (XREF_FIG D)."

reach
"P53 induced the expression of p53-Luc."

reach
"This is in line with some previous evidence showing that p53 phosphorylation is not the main determinant of p53 transcriptional activity as p53 transcription can also be triggered by mere p53 stabilization without phosphorylation."

reach
"Moreover, by using western blot analysis, we determined that EA increased the protein expression of the p53 target proteins p21, p53 upregulated modulator of apoptosis (PUMA) [also known as Bcl-2-binding component3 (BBC3)] and Phorbol-12-myristate-13-acetate-induced protein 1 (NOXA)."

reach
"The combination of increased p53 expression transcriptionally induced by Dox and stabilization of p53 by delanzomib is the underlying mechanism behind the synergy of the combination treatment."

reach
"The control construct was unaffected by all Wrap53 siRNAs, strongly supporting our hypothesis that the first exon of p53 is crucial for Wrap53 mediated regulation of p53.A shorter construct was genera[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"As shown, co expression of p53 + TIS21 clearly induced PTM of p53, but not by the p53 alone expresser."

reach
"At the same time, the expression of p53 which is known as a tumor suppressor and p21 which prevents cell proliferation were increased by 2.5- (p53) (P < 0.01) and 2-fold (p21) (P < 0.01) at 0.1 mug/mL of BA-TPQ-hydrogel treatment, and by 3.9- (p53) (P < 0.01) and 3.2-fold (p21) (P < 0.01) at 0.5 mug/mL of BA-TPQ-hydrogel treatment (XREF_FIG), respectively."

reach
"Western blotting demonstrated that wt-p53 markedly increased p53 levels in normoxic control N2a cells (XREF_FIG)."

reach
"Second, p53 transcriptionally activates the expression of TP53 inducible glycolysis and apoptosis regulator (TIGAR), which dephosphorylates fructose-2,6-bisphosphate to antagonize the early stages of glycolysis."

reach
"Embryo treatment with the ROS producer pyocyanin upregulated Tp53 and pChk1 protein (XREF_FIG), induced expression of tp53 and its target genes (XREF_FIG), and elevated Caspase-3 cleavage (XREF_FIG)."

reach
"These studies revealed that some p53 targeted miRNAs modulate p53 expression and activity through forming a feedback regulation pattern."

reach
"This analysis revealed that the p53 adenovirus increased expression of p53 to levels similar to those observed after NGF withdrawal, but that this elevated expression of p53 had no effect on JNK phosphorylation in sympathetic neurons maintained in 20 ng/ml NGF."

reach
"Expression of deltaNp63 (dNp63), a dominant-negative p53 homologue that can repress transcription of p53 pro-apoptotic target genes, commonly occurs in urothelial carcinoma but not in benign urothelium (29-31)."

reach
"Akt inactivation reduces the degradation of p53, which ultimately leads to the increased expression of p21 and p53, and inhibition of tumor progression, indicating that tanshinone induces apoptosis through the mitochondrial apoptosis pathway and inhibition of the PTEN mediated PI3K and Akt pathway for effectively inhibiting tumor growth."

reach
"Up-regulated p53 then selectively up-regulates the expression of p53 downstream genes, including Delta113p53 and p21 to cause cell-cycle arrest, which results in hypoplasia of the digestive organs in the mutant XREF_BIBR."

reach
"Silencing p53 with p53 small interfering RNA (siRNA) significantly abrogated the p53 dependent gene expression and apoptotic induction."

reach
"Modification of p53 protein with isoLGs diminishes its acetylation and binding to the promoters of p53 target genes causing modulation of p53-dependent transcription."

reach
"When it is activated, p53 half-life is increased and leads to the transcription of p53 target genes XREF_BIBR, XREF_BIBR."

reach
"Co-transfection of the wild-type p53 expression plasmid and p53 siRNA restored cellular p53 expression (XREF_FIG) and largely rescued the MHC class I expression level (XREF_FIG), further supporting the direct role of p53 in regulating MHC class I in response to H1N1 infection."

reach
"In this study, we investigated the function of a p53 regulated lncRNA that we named PURPL (p53 upregulated regulator of p53 levels)."

isi
"In addition, URM1 knockdown downregulated the expression of the apoptosis-related factors JNK1/2 and TP53 and upregulated the phosphorylation of JNK1/2 and P53."

reach
"Stoichiometric phosphorylation of human p53 at Ser315 stimulates p53 dependent transcription."

reach
"As p53 is well integrated in a complex molecular network and involved in a number of positive and negative feedback loops [XREF_BIBR], the activation of p53 can induce the expression of p53 targeting microRNAs."

reach
"In HUVEC, we previously demonstrated that P4 binds to PR, subsequently activating the cSrc/Kras/Raf-1/ERK2 signaling cascade to increase NFkappaB nucleus translocation and binding onto the p53 promoter, which in turn up-regulates the expression of p53, eventually resulting in the inhibition of endothelial cell proliferation."

reach
"This suggests, again, that the increased presence of p53 due to the loss of GAS5 is suppressing apoptosis and even a partial loss of p53 due to knockdown of GADD45A can affect its stability and thus, its suppression of apoptosis."

reach
"Activation of the p53 protein by genotoxic agents causes increased p53 protein levels and p53 dependent transcription of the p21 gene."

reach
"Ad-p53 infusion via IIHTBA promoted the protein expression levels of p53, however, it inhibited the protein expression levels of MMP2 and VEGF, indirectly indicating that the gene may inhibit the growth of liver cancer."

reach
"Treatment of p53 wild-type melanoma cells with the genotoxic agent doxorubicin induces G2-M arrest, inhibitory phosphorylation of cell cycle kinase Cdc2 (CDK1) and enhanced expression of p53 and p21."

reach
"Objective: The present study aimed to explore the expressions of long noncoding RNA (lncRNA) p53 upregulated regulator of p53 levels (PURPL) in different ovarian tissues, and to evaluate the significance of disregulations of PURPL and microRNA-338-3p (miR-338-3p) in epithelial ovarian cancer (EOC)."

reach
"Consequently, when we blocked the expression of miR-1228, p53 was released and enforced the inhibition of miR-1228, thereby promoting the accumulation of p53; however, miR-1228 is upregulated in HCC tissue in which p53 is downregulated, repressing p53 expression to relieve its inhibition on miR-1228 expression in turn, which creates a feedback regulation to ensure persistent low protein levels of p53 to control the malignant phenotype."

reach
"More than half of patients with AML showed P53 protein expression by flow cytometry, P53 increased quantification in 256 AML patients was shown in proteomic profiling."

reach
"Moreover, p53, BPEI, beta-CD/AD, and dox complex released Dox and enabled the expression of p53 gene in a sequential manner, and the released Dox and expressed p53 gene showed successive inhibition of the growth of HeLa cells in vitro."

reach
"Nonetheless, our finding that blocking of potential Wrap53 and p53 RNA hybrids using 2-O-oligonucleotides reduces p53 expression provides solid indirect evidence to support the notion that Wrap53 medi[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Adduction of p53 protein with isoLGs interferes with the p53 binding to the promoters of its target genes and modulates p53-dependent transcription and biological activity."

reach
"The results of immunohistochemical studies and RT-qPCR showed that Ad-p53 upregulated the expression of p53, and downregulated the expression of TGF-beta1 and alpha-SMA."

reach
"In a feedback fashion, they work together to directly inhibit the transcriptional activity of p53 and mediate p53 degradation via ubiquitin dependent proteolysis, as MDM2 possesses an E3 ubiquitin ligase activity and its mRNA expression is stimulated by p53, thus, keeping p53 level and activity marginally detectable in most of normal mammalian cells or tissues."

reach
"P53 gene silencing significantly suppressed p53 and p21 mRNA expression without affecting p27 mRNA expression."

reach
"While silencing of p53 reduced the p53 protein expression, it had no effect on BBR induced FOXO3a."

reach
"In TT cells, shRNA mediated TP53 knockdown substantially blocked basal and MKT-077-induced TP53 levels (XREF_FIG)."

reach
"ING3 activates p53 transactivated promoters and modulates p53 mediated transcription, cell cycle control, and apoptosis XREF_BIBR."

reach
"Direct binding of p53 to target promoters and the effect of over-expression of wt and mt p53 on the epigenetic state of promoters was studied in a non malignant hTERT immortalized breast epithelial cell line, HME1."

reach
"To determine whether the targeted p53-mRNA NPs could induce the expression of therapeutic p53 in p53-null RIL-175 cells, we first checked p53 protein expression after treatment with CTCE-p53 NPs versus SCP-p53 NPs."

reach
"Finally, we demonstrated that SNF5 knockdown inhibits p53 translation via eIF4E and replacement of eIF4E in SNF5-knockdown cells restores p53 expression and cell survival."

reach
"Silencing of p53 in HeLa cells expressing GFP-E2 efficiently inhibited the increase of p53 levels induced by E2 (XREF_FIG, compare lanes 1 and 2, 5 and 6, 9 and 10)."

reach
"In the present study, we identified ginkgo biflavones as potential p53 activators that could enhance p53 protein expression level by inhibiting MDM2 protein expression."

reach
"While the mutant vs WT changes induced between proteins were not generally correlated (highest R 2 = 0.02), the lncRNA PURPL (p53 upregulated regulator of p53 levels 30) was one of the five most significant changes for both KHDRBS2 and A1CF."

reach
"However, p53 re-expression almost completely suppressed expression of Cyclin B1 and inhibited BKM120 mediated activation of MEK1/2, indicating that besides upregulation of p21, reduced MEK1/2 activation and suppression of Cyclin B1 contribute to the inhibition of BKM induced polyploidy by p53."

reach
"The transcription factor, p53, binds to its response elements to activate the transcription of canonical p53 target genes[1]."

reach
"In order to further confirm the additive effects of ONC201 on activation of p53, we transfected both ND and MF CD34 cells with human p53 CRISPR activation plasmid to activate TP53 transcription."

reach
"A typical p53 target gene, CDKN1A, was upregulated by both p53 and CNPY2 in RCC cells, suggesting that CNPY2 increased the expression level of TP53."

reach
"XREF_BIBR, XREF_BIBR Treatment with CRM1 inhibitors results in increased nuclear p53 level, triggering p53 mediated transcription and apoptosis."

reach
"Pharmacological activation of P53 by the MDM2 inhibitor, Nutlin-3, also caused increased P53 dependent transcription and the loss of cells from the inner cell mass."

reach
"In the case of GC B cells, the transcription factor Bcl6, which is highly expressed in GC B cells, represses p53 transcription and downregulates p53 expression."

reach
"PML recruits p53 to nuclear bodies to stimulate transcription of p53 targets, including p53 itself (de Stanchina et al., 2004; Ferbeyre et al., 2000)."

reach
"The current study showed that hypoxia up-regulated the expression levels of bcl-2 and p53 proteins and reoxygenation further up-regulated the expression level of p53, but down-regulated the expression[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"For instance, a study by Smith et al. demonstrated that in vitro transfection of breast cancer cells with a dominant negative TP53 gene construct that reduces the expression of p53 is associated with enhanced 18 F-FDG uptake [XREF_BIBR]."

reach
"Accumulation of the tumor suppressor protein p53 was observed in malignant cells [XREF_BIBR] thus leading to the assumption that mutation in TP53 may cause overexpression of p53 protein [XREF_BIBR, XREF_BIBR]."

reach
"Notably, knockdown of p53 using p53 siRNA inhibited the expression of p53, leading to a significantly decreased apoptotic rate in response to DHM (XREF_FIG and XREF_FIG)."

reach
"Moreover, the change of the sub-cellular localization of cytochrome c was effectively blocked in p53- or Bax-knockdown A549 cells, indicating the dependency of p53 and Bax in emodin mediated apoptosis[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"The authors revealed that DNA damage and p53 activation increased expression of not only a known p53 target, miR-34a, but also a broad set of mature miRNAs, and their accumulation is mediated transcriptionally and post-transcriptionally."

reach
"However, alpha-synuclein aggregation seen in PD leads to its inability to inhibit p53, which will enhance p53 expression and potentially lead to cell death [XREF_BIBR]."

reach
"Therefore, VSV-DeltaM51-encoded functional p53 would be able to drive beneficial expression of p53 regulated anticancer genes."

reach
"Quantitative analysis of p53 targeted gene expression and visualization of p53 transcriptional activity following intratumoral administration of adenoviral p53 in vivo."

reach
"The conditional activation of p53 in VE-cadherin + vascular niche cells by deleting Mdm2 induced the expression of p53 target genes specifically in vascular endothelial cells, resulting in the dilation and collapse of vascular endothelial cells and reductions in perivascular mesenchymal stromal cell numbers."

reach
"It is therefore possible that p53 degradation by the action of E1B-55K/E4 Orf6 complex contributes to the loss of p53 from the L4P subsequent to its activation."

reach
"Wolf D. & Rotter V. (1985) Major deletions in the gene encoding the p53 tumor antigen cause lack of p53 expression in HL-60 cells."

reach
"Moreover, our results suggested that there may be a co-regulation between p53 and Nrf2, in which transfection with siNrf2 induced the p53 expression."

reach
"Our findings unravel distinct patterns of K63 linked ubiquitination of p53 in various cellular compartments, which serves as an important molecular switch that enables p53 transcriptional activation in the nucleus and p53 transcription independent function in the mitochondrial for apoptosis under stress conditions."

reach
"Therefore, amplification of MDM2 or MDM4 as well as repressed p14 ARF or TP53 by promoter methylation can cause reduced p53 expression resulting in a limited sensitivity of IHC [XREF_BIBR, XREF_BIBR]."

reach
"Tenovin-1 (TEN), a p53 activator, increased the nuclear levels of p53 and HNF4alpha."

reach
"AGGF1 antagonizes MDM2 function to inhibit p53 ubiquitination, increases the acetylation, phosphorylation, stability and expression levels of p53, activates transcription of p53 target genes, and regulates cell proliferation, cell cycle, and apoptosis."

reach
"In this study, the presence of Dox in INO80 silencing p53 +/+ HCT 116 cells also increased the protein level of p53 including p53-S15p and p21 (XREF_FIG), suggesting the up-regulation of p21 by INO80 chromatin remodeling complex might be associated with cell cycle phase G2/M checkpoint and abnormal chromosome stability."

reach
"In all cases, there was p53 dependent induction of expression of the p53 target genes mdm2, gadd45, and p21, accompanied by p53 dependent partial restoration of apoptosis."

reach
"P53 levels can be increased directly by Tp53 upregulation and indirectly by Lyn via increased translocation of p53 to the cytoplasm and reversal of Mdm2 mediated degradation of p53."

reach
"On the other hand, sumoylation of DJ-1 and p53 reactivates p53 transcription through a Topors and p53BPs complex."

reach
"53 It has been recently shown that loss of TIF-IA induces acetylation of p53, which in turn leads to stabilization and increased levels of p53."

reach
"Western blotting indicated that p53 expression level was increased by p53 overexpression, but decreased by E6 knockdown in TL-1 cells."

reach
"Because TT cells harbor wild type TP53 and MKT-077 upregulated TP53 levels in these cells (XREF_FIG), we next determined the significance of TP53 for MKT-077-induced growth inhibitory effects using RNA interference."

reach
"P53 mediated downregulation of HMGB2 was confirmed by direct activation of p53 using Nutlin-3 or by inducing p53 expression using Tet-On system."

reach
"Interestingly, we did not recognize an increase of p53 mRNA levels (data not shown), which suggests that the potential p21 mediated effect on the cell cycle may be independent of p53 or that the effect is executed by p21 rather than p53 as already shown in another study [XREF_BIBR]."

reach
"Functionally, p73, a family member of p53, activates transcription of p21- and p53 responsive genes, which participate in cell cycle control, DNA repair, and apoptosis and inhibits cell growth in a p53 like manner by inducing apoptosis or G1 cell cycle arrest XREF_BIBR, XREF_BIBR."

reach
"XREF_BIBR Therefore, the level of p53 can be modulated both by the ATM dependent pathway and the mutational status of p53 itself."

reach
"In U87 glioma cells and GSCs, resveratrol reduced AKT phosphorylation and induced p53 expression and activation that led to transcription of downstream p53 target genes."

reach
"In response to cellular stresses such as DNA double-strand breaks, hypoxia, and inappropriate oncogene activation, the p53 transcription factor is stabilized within the cell and induces the expression of various p53 target genes involved in cell cycle arrest, apoptosis, and senescence."

reach
"As already seen for other wtp53 lines, 17AAG treatment of HCT116 p53 +/+ increased their p53, p21 and PUMA protein levels but decreased their MDMX levels (XREF_FIG)."

reach
"m-NAD (P)-ME depletion in A375 melanoma cells (wild-type p53) enhanced AMPK activity, increased p53 levels, and up-regulated the p53 downstream target p21, which inhibits the cell cycle."

reach
"In this particular IR response, P53 dependent cell death was revealed by not only increased p53 target gene expression, but also enhanced protein protein interaction between P53 and other proteins XREF_BIBR - XREF_BIBR."

reach
"Focusing our attention on P53, a critical modulator of Bcl2 family members , we have found that: (i) TP53 mRNA overexpression in HTLA-ER cells is not accompanied by an increase in the protein level (Figs 2 and 3 supplementary); (ii) the amount of P53 protein is high and not inducible in either cell line (Fig. 2); and (iii) MDM2, the primary negative regulator of P53 (Fig. 4 supplementary) , is not amplified in either cell population."

reach
"27 Even in the absence of keratinocytes, there is a strong melanogenic response to UVR mediated by p53 in human melanocytes and melanoma cells in vitro, which can be explained by the fact that p53 regulates the transcription of the hepatocyte nuclear factor 1alpha (HNF1 alpha), a tyrosinase transcription factor, in melanocytes."

reach
"A similar assessment of TP53 using RNA also showed equal expression of mRNA for endogenous TP53 (TP53 3’ UTR) and increased total TP53 expression (TP53 total; Revised Supplemental Figure 3D, 3E, left)."

reach
"The prime goal of p53 based cancer therapy has been to increase levels of functional p53 and/or inhibit MDM2 levels to prevent further p53 degradation."

reach
"Although it was not possible to sequence the TP53 gene in all 253 patient samples, IHC for p53 is a proxy for its mutational status, as most deleterious mutations in TP53 typically cause complete loss or destabilization of p53 protein expression, leading to an " all or nothing " IHC staining pattern."

reach
"Then, Bach2 serves as a crucial mediator to execute the negative selection of early B cells with failed V H -DJ H rearrangements by binding to the promoter of ARF/TP53 to induce the p53 expression, which in turn mediates a program of cell death [28, 29] ."

reach
"We found that the protein expression of PI3K, p-AKT1 (S473), AKT1, and p53 in nuclei and cytoplasm showed that H O increased nuclear p53 protein level but decreased the protein levels of PI3K, p-AKT1 (S473), and AKT1; in contrast, this effect was rescued by overexpression of IGF-1 with adenovirus vector via activating the PI3K/AKT1 pathway in cytoplasm (Fig. 6a)."

reach
"Apoptogenic effect was more pronounced in functional p53 expressing cells in which theaflavins raised p53 protein levels that harmonized with Bax up-regulation and migration to mitochondria."

reach
"These results may be due, in part, to the presence of immune cells, such as activated lymphocytes expressing WT p53 at levels comparable to some tumor cells, wherein further increase of p53 expression by p53 SMWC may alter survival of these immune cells and negatively impact an effective immune response."

reach
"In the present study, the reduced protein level of p-p53 and mRNA levels of the downstream genes of p53 in 9935-overexpressed NRK-52E cells suggested that 9935 overexpression inhibited p53-mediated transcription-dependent apoptosis."

reach
"Methylation, mutations, and CNAs have complicated effects on downstream gene expression: for instance, inactivating mutations in TP53 can increase TP53 levels through a feedforward mechanism (Marks et al., 1991; Rodrigues et al., 1990), while certain chromosomal amplifications can fail to increase protein expression due to dosage compensation (McShane et al., 2016; Schukken and Sheltzer, 2021)."

reach
"Wolf, D. and Rotter, V. : Major deletions in the gene encoding the p53 tumor antigen cause lack of p53 expression in HL-60 cells."

reach
"SETD2 increases the binding of H3K36me3 to the p53 promoter that enhances the expression of p53."

reach
"Loss of wild-type p53 reduces expression of the p53 target gene BTG2, which normally represses Hras by reducing its GTP-loaded state [135]."

reach
"P53 promotes G1 cell arrest through its ability to induce p21 expression XREF_BIBR, and p53 and p21 levels are increased by nutlin-3a treatment in wild-type p53 glioblastoma cells."

reach
"In contrast, upon Ash2L pre-silencing, P53 induction resulted in complete abrogation of H3K4me3 mark and reduced P53 pro apoptotic gene expression without affecting P53 occupancy on these same promoters."

reach
"TP53 knockdown reduced SOX2 mRNA and protein expression, whereas SOX2 knockdown did not affect TP53 expression levels, indicating a regulatory role of TP53 on SOX2 in NSCLC."

reach
"However, transfection of p53 not only restored the expression of p53 but also increased the endogenous DDB2 level (lane 2)."

reach
"As an essential transcription factor, p53 activates expressions of p21 Waf1 and Cip1, Bax, Puma, FAS and other p53 responsive genes, consequently promotes cell growth-arrest, apoptosis, DNA repair and cell differentiation."

reach
"N-acetyl L cysteins and pifithrin-alpha, an inhibitor of p53 abrogated glucolipotoxicity induced ROS generation and p53 expression."

isi
"All PCCs harbored KRAS and TP53 mutations, and expressed cytokeratin 19, ki-67, and p53, while the expression of EpCAM and vimentin was variable."

reach
"Notably, p63 and p53 data was collected from cells with strong basal p63 expression and stimulated p53 expression, respectively."

reach
"In addition to p53 regulating gene expression directly by binding to genes’ p53 RE, p53 modulates gene products through the induction of miRNAs that are controlled by p53 at the transcriptional level."

reach
"In addition, the KDM4C inhibition in p53 depleted cells abrogated the induction of p53 apoptotic gene expression caused by KDM4C inhibition."

reach
"However, our observation that liver of Pirh2 -/- mice display elevated IR induced p53 expression compared to Wt controls whithout any detectable level of p19 ARF, supports that increased IR induced p53 expression in Pirh2 mutants can take place independently of p19 ARF."

reach
"Infection of p53 TR/TR or Rb TR/TR MEFs with adenoviral FlpO (mammalian codon optimized Flp) effectively restored p53 and Rb expression to levels indistinguishable from those in p53 XTR and XTR or Rb XTR and XTR MEF lines (XREF_FIG)."

reach
"Conversely, reconstitution of wild-type p53 (p53 wt) in HK301 and HK336 p53KO cells displayed similar localization as parental cells and rescued transcription of p53 regulated genes (XREF_FIG and XREF_SUPPLEMENTARY)."

reach
"The fourth intron of p53 is known to increase expression of p53 in a position dependent manner."

reach
"Quantitative analysis of p53 mRNA in PCBP2-downregulated cells revealed a lower level of p53 mRNA under normal conditions suggesting the involvement of PCBP2 in p53 mRNA stabilisation."

reach
"In the present study, an immunohistochemical assay revealed that in a model of CCl 4 -induced hepatic fibrosis, Ad-p53 significantly decreased the positive expression of TGF-beta1 and alpha-SMA, and increased the positive expression of p53 compared with the normal saline group."

reach
"Pifithrin -α, an inhibitor of p53, can reduce the level of nuclear p53 and reduce the activity of caspase3, thus alleviating apoptosis and necrosis (Schafer et al., 2003)."

reach
"Immunoblotting analysis revealed that the p53 protein level was increased by approximately threefold relative to its endogenous levels in islets of mice infected with AAV encoding FLAG tagged DN-p53."

reach
"A transversion missense polymorphism of the TP53 tumor suppressor gene at the codon 72 codes proline instead of arginine causes an altered p53 protein expression and has been found to be associated with an elevated risk of various cancer; especially breast and lung cancer."

reach
"Wolf, D. and Rotter, V. : Major deletions in the gene encoding the p53 tumour antigen cause lack of p53 expression in HL-60 cells."

reach
"p21, a transcriptional target of p53, was considered to reflect p53 activity and could decrease false positive results of p53 expression [XREF_BIBR]."
| PMC

reach
"These findings suggest that p53 interacts with ELL and interferes with the binding of ELL to ICE1, raising the possibility that p53 inhibits the recruitment of LEC to MED26 and decreases the expressio[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

reach
"Functional alterations of p53 were evaluated by exploring endogenous and induced p53 expression with Western blot analysis."

reach
"Interestingly, a recent study has suggested that chalcone derivatives disrupt the MDM2 and p53 protein complex to cause increased p53 levels (Stoll et al., 2001)."