"Further, while RasV12 induced the expression of Cdc6 and p53, IKKbetaca, acting together with RasV12, suppressed their expression to levels similar to those detected in IMR-90 IKKbetaca cells; and Cdc6 expression levels in IMR-90 IKKbetaca cells appeared to be comparable to their empty vector controls (XREF_FIG)."
"RasV12 failed to induce p53, p19ARF, p16INK4a, and p15INK4b expression in KSR1-/- MEFs and increased proliferation instead of causing growth arrest."
"Thus, while RasV12 induced the expression of the p53 - p21 Cip1 and Waf1 axis, IKKbetaca only contributed to p21 Cip1 and Waf1 induction, which has been reported to be a target of activated canonical NF-kappaB in other cells."
"Analysis of cell cycle regulatory proteins in confluent cells showed that overexpression of RasV12 induced the expression of cyclin D1 and Cdc6 but also of p53 and its target protein p21 Cip1 and Waf1."
"Similarly, overexpression of RasV12 induced the expression of p53 and also triggered its stabilizing phosphorylations at Ser15 and Ser20."
"Importantly, enforced expression of IKKbetaca suppressed both the basal and RasV12 induced expression levels of p53 in IMR-90 fibroblasts (XREF_FIG)."