IndraLab

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KRAS increases the amount of KRAS. 10 / 56
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"Compared to the unmodified WT cells, KI of KRAS enhancer variant (chr12 :25538881 : C> T) increased the baseline KRAS expression in DMSO treated KI-Mut cells (2.7 and 2.2-fold, P < 0.001; XREF_FIG)."
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"23 However, the KRAS variant is associated with a BRCA1 mutation like gene expression signature, supporting the notion that there might be increased oncogenic risk in the presence of the KRAS variant and high KRAS expression and low BRCA1 expression, either through mutation or other mechanisms."
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"Liu et al. confirmed that KRAS gene SNP rs8720 CT increased mRNA and protein expression of KRAS and thus contribute to increased colorectal carcinoma (CRC) risk and reduced survival (41)."
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"Western blotting revealed that the expression of KRAS and beta-catenin was eliminated by DDX3 or KRAS silencing, but KRAS expression was unchanged by LY294002, shDDX3 plus KRAS or shDDX3, LY294002 plus KRAS treatments in CCM2 cells."
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"Previous studies have provided preliminary evidence that the KRAS G4 can indeed be targeted with small molecules to elicit changes in KRAS expression."
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"reported interaction between the Myc associated zinc-finger protein MAZ and the G-quadruplex motif present in the promoter of murine KRAS resulting in activation of KRAS expression."
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"A fraction of oncogenic Kras induced T-ALL and MPN lose wild-type Kras expression."
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"Notably, overexpression of exogenous WT KRAS did not elicit additional tumor-suppressive effects in pancreatic cancer cells with endogenous WT KRAS expression (Fig. S4), signifying that it’s the presence of the WT allele, more than the dosage, that is more pertinent to its tumor-suppressive functions."
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"In contrast, while knockdown of wild-type KRAS did not significantly reduce KRAS protein expression in H358 cells, mutant specific knockdown potently and specifically reduced KRAS protein expression."
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"Indeed, there was a decent reduction in c-MYC nuclear stain in case of C-KRAS-esiRNA treatment of DLD1 tumors (XREF_FIG), meanwhile KRAS levels were also reduced by KRAS knockdown (XREF_FIG)."
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Modified KRAS increases the amount of KRAS. 4 / 4
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"Induction of KRAS shRNA by addition of DOX reduced KRAS protein levels (XREF_FIG, red arrow) and withdrawal of DOX restored KRAS expression to almost normal levels within 48 hours (XREF_FIG, green arrow) suggesting inducible and reversible knockdown of KRAS in these cells (note that the KRAS western blots shown in XREF_FIG were probed with an anti-KRAS antibody that binds to both wildtype and mutant KRAS)."
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"Notably, however, overexpression of the 3 ' UTR of the KRAS pseudogene KRAS1P increases KRAS levels and promotes cells growth."
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"KRAS mRNA expression in these cells was increased up to 60-fold compared to control cells, illustrating that forced expression of mutant cDNAs using viral vectors often results in supraphysiologic KRAS levels, which complicates assessment of the biochemical and functional consequences."
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"As seen in Figures XREF_FIG and XREF_SUPPLEMENTARY, activation of KRAS transcription during starvation increased the level of wild-type KRAS, associated with an increase in the amount of free GFP generated, hence of autophagy induction."
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KRAS increases the amount of KRAS-G12D. 4 / 4
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"We further explored the effects of p53 restoration in lung cancer type by crossing the p53 LSL allele to the Kras LA2 allele that drives oncogenic Kras G12D expression after a spontaneous recombination event [XREF_BIBR]."
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"Kras shRNA molecules reduce the expression of both WT Kras and Kras G12D and more potent constructs than shKras.54 inhibited the growth of AML 101-R (data not shown)."
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"The lung epithelium of BRaf CA/+ or KRas LSL/+ littermates was infected with 10 7 pfu of Ad-Cre to initiate expression of BRAF V600E or KRAS G12D oncoproteins respectively."
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"Here we report that Kras LSL-G12D/+; Pdx1-cre, a mouse model known for pancreatic cancer, also mediates Kras G12D expression in the stomach, causing gastric hyperplasia and metaplasia prior to the pathologic changes in the pancreas."
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KRAS-G12D increases the amount of KRAS-G12D. 3 / 3
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"The propensity of Kras G12D HSCs to generate T-ALL led us to investigate the effects of Kras G12D expression in early T-lineage cells."
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"Oncogenic KRAS is a key driver of human lung adenocarcinoma, and early stage lung tumors can be modeled using LoxP-Stop-LoxP Kras G12D knock-in mice (Kras LSL-G12D/+) in which expression of Cre in lung epithelial cells leads to the expression of oncogenic Kras G12D 18,19."
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"Using the CCSP Cre mouse to specifically activate expression of the oncogenic Kras G12D in Club cells, we investigated the expression of Mig-6 in CCSP Cre Kras G12D -induced lung tumors."
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Mutated KRAS increases the amount of mutated KRAS. 3 / 3
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"A comprehensive proteomic analysis of these exosomes showed that, depending on cell source, they contained very different constituents, with mutant KRAS cell derived exosomes having increased levels of SRC, EGFR and mutant KRAS compared to exosomes from wild-type KRAS DKs-8 cells."
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"As shown in Figure 1A and quantified in Figure 1B, knockdown of Smurf2 in mutant KRAS driven lung adenocarcinoma cell lines [H358 (KRASG12C) and H441 (KRASG12V)] caused ~ 60% to 80% reduction in mutan[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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"To examine the role of TIGAR in the development of PDAC, we utilized well-established mouse models that use Pdx1-Cre to drive pancreas expression of mutant KRAS (LSL-Kras ) alone (KC) or mutant KRAS with mutant p53 (LSL-p53 ; KPC) or with loss of p53 (p53 ; KFC)."
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Mutated KRAS increases the amount of KRAS. 3 / 3
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"We observed that mutant KRAS modestly suppressed p53 protein expression but increased p21 protein expression at the highest levels of KRAS activation (Fig. 3A)."
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"In H1650 mutant EGFR cells, mutant KRAS knockdown also reduced KRAS protein levels but without a significant effect on the downstream signal transduction pathways."
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"As reported earlier, we also observed that siRNA mediated downregulation of beta-TrCP1 in mutant KRAS driven H358 and H441 cells enhances KRAS steady-state level; overexpression of beta-TrCP1 causes d[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
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GTP-bound active KRAS increases the amount of KRAS bound to RASSF2. 1 / 1
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"RASSF2 binds directly to K-Ras in a GTP-dependent manner via the Ras effector domain."
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Modified KRAS increases the amount of KRAS-G12D. 1 / 1
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"Diminished pancreas desmoplasia, enhanced cancer cell apoptosis, suppressed cancer cell proliferation, reduced phospho-ERK, phospho-AKT and Kras levels are noted in KTC tumors, as well as diminished oncogenic Kras G12D expression with iExosomes treatment (XREF_FIG, XREF_FIG)."
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Modified mutated KRAS increases the amount of KRAS. 1 / 1
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"Overexpression of mutant KRAS (pCMV6-KRAS G12D, Figure XREF_FIG a) led to increased expression of KRAS, CIP2A, and pERK, and increased cell proliferation."
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KRAS-G12D increases the amount of KRAS. 1 / 1
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"However, in this model, KRAS G12D cooperated with PTEN silencing to induce thyroid cancer indicating that KRAS is expressed in mouse thyrocytes."
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