"As shown in XREF_SUPPLEMENTARY, activation of Akt by expressing a constitutively active C/A-Akt indeed induced expression of PAI-1 and CTGF in Smad3 +/+ cells, but not in Smad3 -/- cells."
"Inhibition of the PI3K and Akt pathway abrogates TGF-beta-induced PAI-1 expression by disrupting the TGF-beta-induced interaction of transcriptional coactivator and acetyltransferase CBP with activated Smad3."
"In contrast, constitutively active PI 3 kinase and Akt kinase increased PAI-1 expression, similar to TGFbeta."
"An expanded analysis of the murine fibrosarcoma cells discussed above revealed that PAI-1 deficient fibrosarcomas have reduced Akt signaling and reintroduction of PAI-1 expression in deficient cells results in an increase in Akt signaling [XREF_BIBR]."
"These findings indicate that p38- and PKB dependent signalling pathways contribute to enhanced PAI-1 levels in the hypoxic response."
"Indeed, direct activation of Akt by expressing a constitutively active form of C/A-Akt induces expression of fibrotic response gene PAI-1 and CTGF in Smad3 +/+ cells, but not in Smad3 -/- cells (XREF_SUPPLEMENTARY)."
"Furthermore, the administration of Akt activity inhibitors greatly diminished the expression of NF-kappaB and PAI-1."