"In our study, we found that PI3K-AKT-mTOR pathway proteins in normal rats were expressed at low levels, while there was increased expression of p-AKT, p-mTOR and p-P70S6K in brain tissues of anaesthetized rats treated with PI3K and Akt agonists."
"Although not addressed by the present study, it is likely that Akt, which is activated by ischemia, 49 activates p-mTOR at S2448."
"Since Akt promotes oncogenesis by inhibiting apoptosis and promoting proliferation of cancer cells directly as well as indirectly by regulating the activation of NF-kappaB and p-mTOR, we investigated the mechanism by CDDO-Me inhibits Akt."
"As expected, the MAPK inhibitor U0126 effectively decreased p-ERK1/2 protein expression (0.01 +/-0.01-fold versus 1 +/-0.2-fold vehicle treated, P < 0.001), and the PI3K inhibitor LY294002 decreased p-Akt (0.01 +/-0.01-fold versus 1 +/-0.09-fold vehicle treated), downstream Akt target p-mTOR (0.5 +/-0.2-fold versus 1 +/-0.2-fold vehicle treated), downstream mTOR target p-70S6 kinase (0.02 +/-0.02-fold versus 1 +/-0.1-fold vehicle treated)."
"Moreover, the combined inhibition of both p38 and Akt in CsA treated animals was more effective and more significantly reduced p-Akt (p = 0.0001), p-p38 (p = 0.02) and p-mTOR as compared to CsA (alone)-treatment group (XREF_SUPPLEMENTARY)."
"This is in sharp contrast to Hs578T cells where a distinct AKT signal fails to activate p-mTOR S2448 and an activation of pRSK S380 is not promoted in this cell line."