"NGF significantly increased total APP protein levels following 96 h of treatment and this increase was prevented in cells pre-treated with S-MIU."
"The levels of all three isoforms of nitric oxide synthase (NOS) are also increased following treatment with NGF [XREF_BIBR, XREF_BIBR] and we found that NOS inhibitors attenuate the increase in APP protein levels caused by NGF [XREF_BIBR]."
"In PC12 cells, a rat pheochromocytoma cell line, we have demonstrated that nerve growth factor (NGF) induces the APP gene expression and increases APP mRNA levels in the presence of 0.5 or 15% serum."
"These results demonstrate that NGF increases APP gene expression at transcriptional level."
"These data suggest that, NGF may elevate APP levels in part through enhanced translation."
"These findings lead us to hypothesize that APP and TrkA only form a complex when APP is not phosphorylated at the T668 site and that, in turn, the reduction in the APP pT668 level induced by NGF facilitates TrkA binding to APP."
"However, in vivo administration of NGF to the developing hamster brain was shown to induce the expression of the beta-amyloid precursor protein (beta APP) gene."
"While NGF did increase APP protein levels, steady-state APP mRNA levels were decreased in NGF treated PC12 cells."
"In fact, it was shown that NGF acts on the APP promoter mediated by p75 NTR and upregulates APP transcription and the secretion of secreted amyloid precursor protein (sAPP), although intraparenchymal NGF delivery did not significantly increase Abeta deposition in monkeys."
"The present study demonstrates that the NGF mediated increase in APP protein levels is not due to enhanced mRNA or protein stability and that NO, which is necessary for NGF induced neurite outgrowth and increased APP protein levels in PC12 cells, modulates APP mRNA levels and protein stability."