"Silencing Mdm2 or Akt1 upregulated p21 expression, whereas Akt1 overexpression downregulated p21 at the promoter and protein levels in p53WT cells."
"Mdm2 has been shown to negatively regulate p21 protein levels in a p53 independent manner, which can account for this increase in the p53-null H1299 line."
"As a confirmation of the effect of Nutlin3A, the data showed that exogenous HDM2 mediated decrease in P53 and P21 expression was partially blocked by Nutlin3A (XREF_FIG)."
"Overexpression of hdm2 resulted in a decrease in the level of p21waf1"
"Next, we tested whether the inhibitors of Wip1 and Mdm2 are increasing p21 and Mdm2 levels in a p53 dependent fashion."
"As expected, inhibition of MDM2 with RG7388 induced p21 WAF1 and CIP1 expression by reactivating p53 in LNCaP cells due to strong inhibition of MDM2 [XREF_BIBR]."
"MDM2 shRNA1 knockdown increased p53 and p21 expression in wild-type p53 cell lines, induced p27 expression in MESO428 and JMN1B, and decreased cyclin D1 expression in MESO428 and MCF7, but had no effect on cyclin D1 expression in MESO924, MESO296, and JMN1B."
"Although comparable in level to that achieved by treatment with the p53 activators actinomycin D and nutlin-3, the increases in p53 and p21 after downregulation of Mdm2 were not sufficient to trigger cell cycle arrest."
"It is known that the expression of p21 is negatively regulated by MDM2 and AKT 36."
"Furthermore, specific inhibition of AKT with triciribine or knockdown of MDM2 with small interfering RNA largely restored p21 expression in PSMC5-knockdown H460 cells."