IndraLab

Statements

IL6 phosphorylates STAT3. 10 / 1926
1 | 10 784 1105 20 2
reach
"Activation of STAT3 phosphorylation by IL-6 treatment was confirmed in a dose dependent assay."
24655440
sparser
"Here, we demonstrated that despite the propensity to produce more IL-6 from APC ( xref ), IL-6 induced STAT3 phosphorylation in neonatal T FH cells was reduced compared to those of adult cells, a likely consequence of the reduced expression of the GP130 component of IL-6R complex on neonatal T FH cells."
30619375
sparser
"These approaches resulted in PN-1974 ( xref ), which had an IC 50 of 126 nM in the competitive ELISA and an IC 50 of 3.3 μM in a cell-based assay based on inhibition of IL-6-induced STAT3 phosphorylation in U937 cells."
26555695
rlimsp
"ATP also activates IL-6 production and phosphorylation of Stat3 that promotes neuron survival."
17524392
sparser
"These results suggested that ASP exerted an inhibitory effect on IL-6-induced STAT3 phosphorylation and hepcidin activation, possibly by inhibiting JAK2 activation."
29147463
sparser
"Western blot analysis of STAT3 phosphorylation showed that the gp130 antibody completely blocked IL-6-induced STAT3 phosphorylation as expected (Fig xref )."
19267906
reach
"Classically, IL-6 signals through binding to the IL-6 receptor (IL-6R) which in turn associates with the dimerized gp130 receptor subunit gp130 to form a receptor complex that activates Janus kinases and triggers STAT3 phosphorylation."
32902739
sparser
"L61H46 inhibited IL-6-induced STAT3 phosphorylation and STAT3 nuclear translocation."
29606890
reach
"However, the kinetics of Stat3 phosphorylation by IL-6 trans-signaling was different than classical IL-6 signaling."
26001777
sparser
"Meanwhile, in another study, IL-6 may also have caused phosphorylation of STAT3, MAPK, and PI3K. xref This recent study may correlate with our results of PI3K genes being downregulated in real-time PCR analysis."
24944518
IL6 leads to the phosphorylation of STAT3 on Y705. 10 / 167
4 | 80 80 2
reach
"IL-6 activates the JAK and STAT signaling pathway and leads to phosphorylation of Tyr 705 of the STAT3 protein [XREF_BIBR]."
22236378
reach
"Next, to determine if STAT3 activation is sufficient to induce FTO-regulated ISGs, we treated Huh7 cells with IL-6, which induces Y705 phosphorylation of STAT3 [30]."
34537236
sparser
"The IL-6 induced constitutive phosphorylation of STAT3 Y705 was effectively suppressed by raloxifene in a dose-dependent manner (Fig.  xref b)."
32940862
sparser
"Pretreatment of ECs with Mag dose dependently inhibited IL-6-induced Tyr705 and Ser727 phosphorylation in STAT3 without affecting the phosphorylation of JAK1, JAK2, and ERK1/2."
16520748
sparser
"Hence, IL-6 is generally known to activate JAK/STAT3 pathway regardless of binding to soluble or membrane bound IL-6R. In line with this, we show that both classic- and trans-signaling of IL-6 trigger STAT3 Tyr705 phosphorylation in a dose- and time-dependent manner."
30185178
sparser
"When HepG2 cells were held at 15 °C, the rate of IL-6 induced STAT3 Y705 phosphorylation was noticeably delayed ( xref )."
21619877
sparser
"Even more surprisingly, the co-treatment of VD3 and IL6 induced significant phosphorylation of STAT3 Tyr705 at 24 h post-treatment that was not induced by the single treatments ( xref )."
33618332
sparser
"Phosphorylation at Tyr705 in STAT3 is induced by IL-6 and IL-23, involves the PI3K-Akt-mTORC1 pathway and controls Th17 commitment and function ( xref , xref )."
29237778
reach
"Studies have shown that IL-6 induces STAT3 phosphorylation on both Tyr705 and Ser727 [XREF_BIBR, XREF_BIBR]."
27074565
sparser
"STATTIC (80 μM), a small molecule inhibitor that binds the STAT3 SH2 domain to inhibit phosphorylation [ xref ], abolished IL-6 induced STAT3 Y705 phosphorylation following IL-6 treatment ( xref )."
21619877
IL6 phosphorylates STAT3 on tyrosine. 10 / 70
| 1 59 5 5
reach
"IL-6 stimulation led to tyrosine phosphorylation of STAT3 (data not shown)."
| DOI
reach
"Because IL-6 has been found to increase the levels of serine pSTAT3 and induce the phosphorylation of STAT3 on tyrosine residues XREF_BIBR, we incubated CLL cells with 30 mg/ml rhIL-6 for 20 minutes and analyzed their miR-155 expression levels at different times following incubation using RT-PCR and relative qRT-PCR."
23750211
reach
"As shown in XREF_FIG d, IL-6 did not induce tyrosine phosphorylation of STAT-3 in these T cells."
12370259
reach
"Physiologically, STAT3 is phosphorylated first by the IL-6 receptor; in its tyrosine phosphorylated form, STAT3 dimerizes and migrates to the nucleus, where it binds to its DNA response elements."
12710894
reach
"Interleukin-6 (IL-6) induces tyrosine phosphorylation and activation of the latent transcription factor Stat3 in HepG2 cells."
8626374
reach
"We also confirmed that 8226 cells lack constitutively activated STAT3, whereas IL-6 stimulated the tyrosine phosphorylation of STAT3 and the activation of MAPK, as previously described."
21164517
sparser
"Mechanistically, FXT suppressed the IL-6-induced phosphorylation of tyrosine residue (Tyr705) of signal transducer and activator of transcription 3 (STAT3) probably by binding to STAT3."
31080186
reach
"Whereas IL-6 stimulated tyrosine phosphorylation of STAT3 in a time- and dose dependent way, DXM and BTM had no effect on STAT3 phosphorylation."
20693312
sparser
"The IL-6 (10 ng/mL)-induced phosphorylation of JAK2 and STAT3 tyrosine residues was significantly inhibited after the cells were treated for 30 min."
33505935
rlimsp
"Representative western blot analysis showing that AG490 (50 μM) and S3I-201 (300 μM) suppressed the tyrosine phosphorylation of STAT3 by IL-6."
24520293
IL6 leads to the phosphorylation of STAT3 on S727. 10 / 34
2 | 16 16
sparser
"However, EB did not inhibit the IL-6-induced Ser727 phosphorylation of STAT3 ( xref ), suggesting that EB inhibited STAT3 activation through inhibiting Tyr705 phosphorylation of STAT3."
26010889
reach
"Transfection of cells with dominant negative STAT3 (S727A) mutant did not affect basal STAT3 (S727) phosphorylation but the STAT3 (S727) phosphorylation and translocation in response to IL-6 was lost (XREF_FIG)."
25740948
sparser
"Recently, we have demonstrated that IL-6 induced STAT3 Ser727 phosphorylation and that transactivation requires activation of the GTPase Rac-1 [25] ."
11322950
sparser
"Vav, Rac1, MEKK1, and SEK but not JNK or ERK1 have been implicated as components of a pathway that leads to serine 727 phosphorylation of Stat3 by IL6 in HepG2 cells [65] ."
11728826
sparser
"Furthermore, HMC was also able to suppress the IL-6 induced phosphorylation of STAT-3 at serine 727 and tyrosine 705 residues in K-562 cells ( Fig. 6 D)."
26291658
sparser
"Pretreatment of ECs with Mag dose dependently inhibited IL-6-induced Tyr705 and Ser727 phosphorylation in STAT3 without affecting the phosphorylation of JAK1, JAK2, and ERK1/2."
16520748
sparser
"Studies have shown that IL-6 induces STAT3 phosphorylation on both Tyr705 and Ser727 [ xref , xref ]."
27074565
sparser
"Sanguinarine inhibits constitutive as well as IL6-induced phosphorylation of Stat3 at both Tyr705 and Ser727 in prostate cancer cells."
21538419
sparser
"Similar results were subsequently reported for IL-6-induced S727 phosphorylation of STAT3 ( xref )."
23352233
sparser
"IL-6 induces STAT3 phosphorylation at Tyr-705 and Ser-727 through IL-6 receptors and JAK to activate STAT3 signalling in HCC cells."
26061710
Modified IL6 leads to the phosphorylation of STAT3. 10 / 20
| 20
reach
"We also conclude that blocking alpha7nAchR reduces the phosphorylation of STAT3, increases the expression of TNF-alpha and IL-6, aggravating viral myocarditis."
25396421
reach
"Overexpression of pro inflammatory cytokines IL-6 and IL-23 stimulated STAT3 phosphorylation in Treg cells."
32151956
reach
"Studies found that moderate levels of TNF-alpha or IL-6 can increase the phosphorylation of STAT3 at Try705, and this is Brg1 dependent [XREF_BIBR, XREF_BIBR]."
23853776
reach
"Given that the amounts of IL-6 and IL-10 by Pam3CSK4 alone treated macrophages was unable to induce STAT3 phosphorylation (XREF_FIG), it is highly unlikely that sustained phosphorylation of STAT3 in STAT1-null macrophages involved dysregulated production of IL-6 and/or IL-10."
26299368
reach
"Because STAT3 activation through IL-6 has previously been implicated in mediating Teff resistance, we also investigated the surface expression of IL-6R as well as IL-6- and TCR mediated phosphorylation of STAT3 in T cells from our study subjects."
30006377
reach
"Next, we tested whether these levels of IL-6 are sufficient to induce STAT3 phosphorylation in muscle fibers."
28264935
reach
"Hypoxia increased serum IL-6 levels, which in turn increased JAK2 and STAT3 phosphorylation, which subsequently upregulated MMP-9."
32047820
reach
"In brain cortex, increased UA : (1) reduced tMCAO induced increases in Vegfa and Mmp9 and Timp1 ratio expressions; (2) induced Sod2 and Cat expressions and reduced MDA levels; (3) induced Il6 expression, upregulated STAT3 and NF-kappaB p65 phosphorylation, induced Socs3 expression, and inhibited microglia activation; and (4) ameliorated the Bax and Bcl -2 ratio and induced a reduction in caspase-3 cleavage as well as in TUNEL positive cell counts."
32959172
reach
"Il-6 overexpression elevated mRNA levels of lipolysis genes, triggered phosphorylation of STAT3, AMPK, and ACC, and increased expression of genes involved in fatty acid oxidation in skeletal muscle."
25660446
reach
"STAT3 phosphorylation can be upregulated by exogenous IL-6, therefore, we sought to determine the effect of bazedoxifene on the expression of IL-6 stimulated STAT3 phosphorylation."
29207075
IL6 leads to the phosphorylation of STAT3 on serine. 9 / 9
| 1 7 1
reach
"Interestingly, the cytokine interleukin-6 also stimulates STAT3 serine phosphorylation, but in contrast to growth factors, this occurs by an ERK independent process."
9343414
reach
"Serine phosphorylation of Stat3 is also induced by interleukin-6 (IL-6) stimulation, which is shown to be independent of mitogen activated protein kinase and sensitive to the Ser/Thr kinase inhibitor H7."
10446219
trips
"Serine phosphorylation of Stat3 is also induced by interleukin-6 (IL-6) stimulation, which is shown to be independent of mitogen-activated protein kinase and sensitive to the Ser/Thr kinase inhibitor H7."
10446219
reach
"IL-6 induces the serine phosphorylation of Stat3 transcription factor (Luttiken et al. 1995, Zhang et al. 1995, Pellegrini and Dusanter-Fourt 1997), also known as acute-phase response factor (APRF) (L[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
9723906
reach
"We now show that IL-6 triggers a delayed phosphorylation of APRF at serine resudues which can be reversed in vitro by protein phosphatase 2A and is also inhibited by H7."
7533107
reach
"Serine phosphorylation of Stat3, induced by IL-6 stimulation, has been shown to be independent of mitogen activated protein kinase and sensitive to the Ser/Thr kinase inhibitor H7."
22162712
reach
"In addition to the tyrosine phosphorylation we have observed that IL-6 also induces a serine phosphorylation of STAT3."
9558728
reach
"As shown in Fig. 2C, IL-6 treatment rapidly activates STAT3 serine phosphorylation (lanes 1-5), the peak effect occurring at 30 min and declining towards control levels after longer (120 min) exposure[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
10486586
rlimsp
"In cultured Schwann cells, we noted that ERK activation is required for the serine phosphorylation of STAT3 by neuropoietic cytokine interleukin-6 (IL-6)."
19885032
IL6 leads to the phosphorylation of STAT3 at position 705. 1 / 1
| 1
reach
"IL-6 increased phosphorylation of STAT3 (at Tyr (705)) and ERK1/2 (at Tyr (204)) within 5 min that peaked at 15-30 min and returned to basal levels at 2 h. Phosphorylation of STAT3 was blocked by genistein, a protein tyrosine kinase inhibitor, and AG490, a JAK2 inhibitor, but not PD98059, an ERK1/2 kinase inhibitor."
12595539