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phosphosite cbn pc11 biopax bel_lc signor biogrid tas lincs_drug hprd trrust | geneways tees isi trips rlimsp medscan sparser reach
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MTOR phosphorylates EIF4EBP1. 10 / 638
1 | 9 59 239 310
rlimsp
"During loss of mTOR activity or the gene silencing of mTOR, phosphorylation of mTOR, p70S6K, and 4EBP1 is minimal or absent during administration of EPO with OGD, demonstrating that EPO relies upon mTOR to phosphorylate p70S6K and 4EBP1 (Figure 2A)."
sparser
"This observation raises the possibility that kinases other than mTOR may phosphorylate 4E-BP1 in SCC cells, including members of the MAP kinase (MAPKs) and cyclin-dependent kinase (CDK) superfamily, as previously reported ( xref - xref ), which may account for the rapamycin insensitivity of the phosphorylation of 4E-BP1."
reach
"We transfected HCC827 cells with a constitutively active mTOR mutant S2215Y that blocks starvation induced mTOR deactivation and dephosphorylation of 4E-BP1."
reach
"mTOR kinase inhibitors have been shown to suppress phosphorylation of 4E-BP1 and global translation more effectively than rapalogs [XREF_BIBR, XREF_BIBR]."
sparser
"For example, mTOR can directly phosphorylate the 36/45 residues of 4E-BP1, and phosphorylation on these residues allows 4E-BP1 to become further phosphorylated on residues such as S64 xref , xref ."
reach
"Previously we have shown that suppression of mTOR activity using 100nM mTOR inhibitor Rapamycin (Rapa) significantly represses phosphorylation of 4E-BP1, however, it does not prevent the oocytes to reach MII stage."
sparser
"mTOR phosphorylates and represses the eIF4E-binding protein 1 (4E-BP1) and induces its dissociation from eIF4E. Free eIF4E can associate with eIF4G for the initiation of cap-dependent translation."
sparser
"Therefore, the TOS motif is required for mTOR-dependent phosphorylation of 4E-BP1 at all known rapamycin-sensitive sites ; this new role of the TOS motif is similar to its role in regulating the phosp[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
reach
"Dissociation of the eukaryotic initiation factor-4E/4E and BP1 complex involves phosphorylation of 4E-BP1 by an mTOR associated kinase."
sparser
"It was found that the phosphorylation of 4E-BP1 by mTOR did not appear to result in its dissociation from eIF-4E, since the majority of labelled 4E-BP1 remained in the m 7 GTP-Sepharose pellet."
MTOR phosphorylates EIF4EBP1 on S65. 9 / 29
1 1 3 7 | 1 9 6
biopax:netpath
No evidence text available
reach
"The RR-mTOR mice also enabled us to demonstrate that mTOR is necessary for mechanical stimulation to induce an increase in 4E-BP1 S64 phosphorylation, and a decrease in total 4E-BP1."
hprd
No evidence text available
hprd
No evidence text available
hprd
No evidence text available
signor
"PHAS-I in adipocytes and HEK293 cells is phosphorylated in the following five sites, all of which conform to a (S/T)P motif (9, 10): Thr-36, Thr-45, Ser-64, Thr-69, and Ser-82. Thr-45 and Ser-64 flank the eIF4E-binding motif (7, 8), and phosphorylation of either site blocks eIF4E binding in vitro (10, 11). Insulin stimulates the phosphorylation of Thr-36, Thr-45, Ser-64, and Thr-69 in both fat cells and HEK293 cells, and incubating cells with rapamycin decreases the phosphorylation of these sites.Immunoprecipitated epitope-tagged mammalian target of rapamycin (mTOR) phosphorylated Thr-36/45. mTOR also phosphorylated Thr-69 and Ser-64 but only when purified immune complexes were incubated with the activating antibody, mTAb1."
sparser
"Hyperphosphorylation of 4EBP1 by mTOR on Ser65 and Thr70 disrupts the binding of 4EBP1 to eIF4E and results in activation of cap-dependent translation [ xref , xref ]."
hprd
No evidence text available
hprd
No evidence text available
MTOR phosphorylates EIF4EBP1 on T46. 10 / 25
1 2 6 | 2 7 7
reach
"4E-BP1 is phosphorylated by mTOR at T37 and T46."
reach
"The mTOR kinase complex 1 (mTORC1), a downstream target of both AKT and ERK signaling [XREF_BIBR, XREF_BIBR], is a master regulator of eIF4E initiated cap dependent translation by phosphorylating 4E-BP1 on Thr37 and Thr46 that act as priming sites for its subsequent phosphorylation on Ser65 and Thr70 [XREF_BIBR]."
reach
"The 4EBP1 protein is directly phosphorylated by mTOR on T37 and T46, which primes subsequent phosphorylation on S65 and T70."
rlimsp
"Taken together, our results suggest that 4E-BP1 phosphorylation by FRAP/mTOR on Thr-37 and Thr-46 is a priming event for subsequent phosphorylation of the carboxy-terminal serum-sensitive sites."
sparser
"4E-BP1 is phosphorylated by mTOR at T37 and T46."
hprd
No evidence text available
hprd
No evidence text available
biopax:phosphositeplus
No evidence text available
reach
"When 4E-BP1 is phosphorylated by mTOR, first at critical priming threonine (T) 37 and T46 residues and then at other sites, 4E-BP1 is inactivated and releases eIF4E to allow initiation of cap dependent translation."
sparser
"Immunoprecipitated mTOR phosphorylates 4E-BP1 on Thr37 and Thr46 in vitro , indicating that mTOR can directly phosphorylate these two sites [ xref , xref , xref ]."
MTOR phosphorylates EIF4EBP1 on T37. 10 / 22
1 2 6 | 7 6
biopax:phosphositeplus
No evidence text available
sparser
"The 4EBP1 protein is directly phosphorylated by mTOR on T37 and T46, which primes subsequent phosphorylation on S65 and T70 ( xref , xref )."
reach
"Taken together, our results suggest that 4E-BP1 phosphorylation by FRAP and mTOR on Thr 37 and Thr 46 is a priming event for subsequent phosphorylation of the carboxy-terminal serum sensitive sites."
hprd
No evidence text available
sparser
"mTOR-dependent phosphorylation of 4E-BP1 at Thr37 and Thr46 primes 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 and, therefore, is an indicator of 4E-BP1 activity ( xref )."
reach
"4E-BP1 is phosphorylated by mTOR at T37 and T46."
reach
"mTOR dependent phosphorylation of 4E-BP1 at Thr37 and Thr46 primes 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 and, therefore, is an indicator of 4E-BP1 activity."
sparser
"Over expression of eIF4E can lead to increased cell proliferation, transformation and tumorigenesis. xref 4E-BP1, known as PHAS-1, normally binds eIF4E, inhibiting cap-dependent translation and is phosphorylated in vivo on multiple residues; phosphorylation by mTOR on Thr37 and Thr46 of human 4E-BP1 may be very important for subsequent phosphorylation. xref EIF4E protein levels were unchanged with the progression of cell series ( xref )."
sparser
"The mTOR-dependent phosphorylation of 4EBP1 on Thr-37 and Thr-46 serves as a priming event that allows a further phosphorylation of Thr-70, Ser-65, and possibly other sites by additional signaling med[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
signor
"Here, we show that a functional TOS motif is required for 4E-BP1 to bind to raptor (a recently identified mTOR-interacting protein), for 4E-BP1 to be efficiently phosphorylated in vitro by themTOR/raptor complex, and for 4E-BP1 to be phosphorylated in vivo at all identified mTOR-regulated sites. mTOR/raptor regulated phosphorylation is necessary for 4E-BP’s efficient release from the translational initiation factor eIF4E. We find that the TOS motif is absolutely required for efficient phosphorylation of 4E-BP1 at all the identified mTOR-regulated sites, namely, Thr37/46, Ser65, and Thr70 in vivo."
MTOR phosphorylates EIF4EBP1 on T70. 10 / 18
1 4 6 | 1 5
signor
"PHAS-I in adipocytes and HEK293 cells is phosphorylated in the following five sites, all of which conform to a (S/T)P motif (9, 10): Thr-36, Thr-45, Ser-64, Thr-69, and Ser-82. Thr-45 and Ser-64 flank the eIF4E-binding motif (7, 8), and phosphorylation of either site blocks eIF4E binding in vitro (10, 11). Insulin stimulates the phosphorylation of Thr-36, Thr-45, Ser-64, and Thr-69 in both fat cells and HEK293 cells, and incubating cells with rapamycin decreases the phosphorylation of these sites.Immunoprecipitated epitope-tagged mammalian target of rapamycin (mTOR) phosphorylated Thr-36/45. mTOR also phosphorylated Thr-69 and Ser-64 but only when purified immune complexes were incubated with the activating antibody, mTAb1."
hprd
No evidence text available
sparser
"mTOR-dependent phosphorylation of serine Ser 65 and N-terminal Thr 37 , Thr 46 , and Thr 70 in 4E-BP1 cleaves 4E-BP1 from the translational activator eIF-4E ( xref )."
signor
"Mammalian target of rapamycin-dependent phosphorylation of phas-i in four (s/t)p sites detected by phospho-specific antibodies."
sparser
"Hyperphosphorylation of 4EBP1 by mTOR on Ser65 and Thr70 disrupts the binding of 4EBP1 to eIF4E and results in activation of cap-dependent translation [ xref , xref ]."
sparser
"mTAb1-activated mTOR phosphorylated Thr36, Thr45, Ser64, Thr69, and Ser82 in PHAS-I. All five of these sites fit a (Ser/Thr)-Pro motif and are dephosphorylated in response to rapamycin in rat adipocytes."
hprd
No evidence text available
hprd
No evidence text available
hprd
No evidence text available
biopax:phosphositeplus
No evidence text available
MTOR phosphorylates EIF4EBP1 on T36. 9 / 13
3 | 4 4 1
rlimsp
"mTAb1-activated mTOR phosphorylated Thr36, Thr45, Ser64, Thr69, and Ser82 in PHAS-I."
sparser
"The present study has confirmed that mTOR (or a tightly associated kinase) phosphorylates 4E-BP1 at T 36 and/or T 45 and has identified an mTOR-associated kinase which phosphorylates 4E-BP1 within a[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
signor
"In response to insulin and nutrients, mTORC1, consisting of mTOR, raptor (regulatory-associated protein of mTOR), and mLST8, is activated and phosphorylates eukaryotic initiation factor 4E-binding protein (4EBP) and p70 S6 kinase to promote protein synthesis and cell size."
rlimsp
"We also measured the phosphorylation of the T36/45 residues on 4E-BP1. Importantly, numerous studies have shown that mTOR can directly phosphorylate these residues [42], [43], but much to our surprise, neither mechanical stimulation, nor U0126, altered the amount of T36/45 phosphorylation."
sparser
"RAFT1 phosphorylation of 4E-BP1 on Thr-36 and Thr-45 blocks its association with the cap-binding protein, eIF-4E, in vitro, and phosphorylation of Thr-45 seems to be the major regulator of the 4E-BP1-eIF-4E interaction in vivo."
rlimsp
"RAFT1 phosphorylation of 4E-BP1 on Thr-36 and Thr-45 blocks its association with the cap-binding protein, eIF-4E, in vitro, and phosphorylation of Thr-45 seems to be the major regulator of the 4E-BP1-eIF-4E interaction in vivo."
signor
"Mtorc1 promotes protein synthesis by phosphorylating the eukaryotic initiation factor 4e (eif4e)- binding protein 1 (4e-bp1) and the p70 ribosomal s6 kinase 1 (s6k1). Raft1 phosphorylation of 4e-bp1 on thr-36 and thr-45 blocks its association with the cap-binding protein, eif-4e,in vitro. in response to insulin and nutrients, mtorc1, consisting of mtor, raptor (regulatory-associated protein of mtor), and mlst8, is activated and phosphorylates eukaryotic initiation factor 4e-binding protein (4ebp) and p70 s6 kinase to promote protein synthesis and cell size."
reach
"RAFT1 phosphorylation of 4E-BP1 on Thr 36 and Thr 45 blocks its association with the cap binding protein, eIF-4E, in vitro, and phosphorylation of Thr 45 seems to be the major regulator of the 4E-BP1-eIF-4E interaction in vivo."
signor
"Specifically as part of mTORC1, mTOR directly phosphorylates the ribo- somal protein S6 kinases (S6K1 and S6K2) and the eukaryotic initiation factor 4E (eIF4E)-binding proteins (4E-BP1 and 4E-BP2), both of which control specific steps in the initiation of cap-dependent translation"
Kinase-active MTOR leads to the phosphorylation of EIF4EBP1. 10 / 13
13 |
bel
"In addition, two proteins that interact with mTOR to target it to two important substrates, eukaryotic initiation factor 4E binding protein 1 and ribosomal protein S6 kinase, have been identified."
bel
"Rapamycin blocks both 4E-BP1 phosphorylation and cap-dependent translation ( [6]), and mTOR has been demonstrated to phosphorylate 4E-BP1 directly (discussed in further detail below) ( [14])."
bel
"Activation of mTOR results in phosphorylation of a variety of substrates, including the eIF4E-binding protein (4E-BP1), and the ribosomal protein S6 protein kinase (S6K1; for review, see Gingras et al. 2001)."
bel
"Activation of mTOR by insulin results in phosphorylation of downstream targets, e.g. 4E-BP1, S6K1 and ribosomal protein S6, resulting in activation of the mRNA-binding steps in translation initiation. Activation of the phosphatidylinositol 3-kinase-mammalian target of rapamycin signalling pathway by amino acids Although it is clear that amino acids, and in particular leucine, enhance phosphorylation of proteins downstream of mTOR, e.g. 4E-BP1 and S6K1, and indeed require mTOR to be active in order to be effective, whether or not they directly regulate mTOR activity is undecided."
bel
"exogenously provided PA stimulated the activation of the mTORsubs trate S6 kinase and phosphorylation of another mTORsubs trate eukaryotic initiation factor 4E binding protein-1 (4E-BP1) in HEK293 cells. The effect of PA was sensitive to rapamycin (3, 8) and was dependent on the presence of amino acids (3). The amino acid dependence indicated that the effect of PA was physiologic. Subsequently, Blenis group similarly showed that PA stimulated S6 kinase activity in HEK293 cells (9). The ability of PA to stimulate S6 kinase was suppressed by coexpression of TSC1/2, also suggesting that the PA-induced S6 kinase activity was physiologic. PA was also shown to activate mTORin macrophages in an Akt-dependent manner"
bel
"This function of Rheb is blocked by rapamycin and dominant-negative mTOR."
bel
"Phosphorylation of 4E-BP1 relieves its binding to and inhibition of eIF-4E, which, along with S6K1 activity, leads to increased protein synthesis and cell growth"
bel
"The mechanism involves the ability of this integrin to stimulate the phosphorylation and inactivation of 4E-binding protein (4E-BP1), a translational repressor that inhibits the function of eukaryotic translation initiation factor 4E (eIF-4E). The regulation of 4E-BP1 phosphorylation by alpha 6 beta 4 derives from the ability of this integrin to activate the PI-3K-Akt pathway and, consequently, the rapamycin-sensitive kinase mTOR that can phosphorylate 4E-BP1."
bel
"This response is mediated by mTOR and is believed to result from 4EBP1 phosphorylation, which allows eIF4E to bind eIF4G."
bel
"When part of a complex that also contains the proteins raptor and GbetaL, mTOR phosphorylates substrates like S6K1 and 4E-BP1."
MTOR phosphorylates EIF4EBP1 on T45. 9 / 11
3 | 1 4 2
signor
"Mtorc1 promotes protein synthesis by phosphorylating the eukaryotic initiation factor 4e (eif4e)- binding protein 1 (4e-bp1) and the p70 ribosomal s6 kinase 1 (s6k1). Raft1 phosphorylation of 4e-bp1 on thr-36 and thr-45 blocks its association with the cap-binding protein, eif-4e,in vitro. in response to insulin and nutrients, mtorc1, consisting of mtor, raptor (regulatory-associated protein of mtor), and mlst8, is activated and phosphorylates eukaryotic initiation factor 4e-binding protein (4ebp) and p70 s6 kinase to promote protein synthesis and cell size."
rlimsp
"Mutational analysis of sites in the translational regulator, PHAS-I, that are selectively phosphorylated by mTOR. Results obtained with PHAS-I proteins having Ser to Ala mutations in the five known phosphorylation sites indicate that mTOR preferentially phosphorylates Thr36 and Thr45."
sparser
"Recent findings suggests that Thr36 and Thr45 in PHAS-I are preferentially phosphorylated by mTOR [14] ."
signor
"In response to insulin and nutrients, mTORC1, consisting of mTOR, raptor (regulatory-associated protein of mTOR), and mLST8, is activated and phosphorylates eukaryotic initiation factor 4E-binding protein (4EBP) and p70 S6 kinase to promote protein synthesis and cell size."
sparser
"The present study has confirmed that mTOR (or a tightly associated kinase) phosphorylates 4E-BP1 at T 36 and/or T 45 and has identified an mTOR-associated kinase which phosphorylates 4E-BP1 within a[MISSING/INVALID API KEY: limited to 200 char for Elsevier]"
sparser
"mTAb1-activated mTOR phosphorylated Thr36, Thr45, Ser64, Thr69, and Ser82 in PHAS-I. All five of these sites fit a (Ser/Thr)-Pro motif and are dephosphorylated in response to rapamycin in rat adipocytes."
reach
"A second major target of mTOR is 4E-BP1, which is directly phosphorylated by mTOR at T36 and T45 [XREF_BIBR]."
reach
"RAFT1 phosphorylation of 4E-BP1 on Thr 36 and Thr 45 blocks its association with the cap binding protein, eIF-4E, in vitro, and phosphorylation of Thr 45 seems to be the major regulator of the 4E-BP1-eIF-4E interaction in vivo."
sparser
"RAFT1 phosphorylation of 4E-BP1 on Thr-36 and Thr-45 blocks its association with the cap-binding protein, eIF-4E, in vitro, and phosphorylation of Thr-45 seems to be the major regulator of the 4E-BP1-eIF-4E interaction in vivo."
MTOR phosphorylates EIF4EBP1 on threonine. 9 / 9
| 3 6
reach
"4EBP1 is phosphorylated by mTOR at serine 65 and threonine 70."
reach
"mTOR phosphorylated PHAS-I on serine and threonine residues in vitro, and these modifications inhibited the binding of PHAS-I to eIF-4E."
reach
"4EBP1 is phosphorylated by mTOR at serine 65 and threonine 70 XREF_BIBR."
sparser
"mTOR phosphorylated PHAS-I on serine and threonine residues in vitro, and these modifications inhibited the binding of PHAS-I to eIF-4E. These studies define a role for mTOR in translational control and offer further insights into the mechanism whereby rapamycin inhibits G1-phase progression in mammalian cells."
reach
"Interestingly, there is evidence that mTOR directly phosphorylates 4E-BP1 at threonine 37/46."
reach
"It was shown that mTOR phosphorylated 4E-BP1 at serine and threonine residues in insulin stimulated human embryonic kidney cells and these phosphorylations are inhibited by rapamycin [XREF_BIBR]."
reach
"When 4E-BP1 is phosphorylated by mTOR, first at critical priming threonine (T) 37 and T46 residues and then at other sites, 4E-BP1 is inactivated and releases eIF4E to allow initiation of cap dependent translation."
sparser
"The interaction of 4E-BP1 with eIF4E is negatively regulated by mammalian target of rapamycin (mTORC1)-dependent phosphorylation of serine and threonine residues on 4E-BP1."
sparser
"It was shown that mTOR phosphorylated 4E-BP1 at serine and threonine residues in insulin-stimulated human embryonic kidney cells and these phosphorylations are inhibited by rapamycin [ xref ]."
MTOR phosphorylates EIF4EBP1 on serine. 8 / 8
| 1 3 4
sparser
"It was shown that mTOR phosphorylated 4E-BP1 at serine and threonine residues in insulin-stimulated human embryonic kidney cells and these phosphorylations are inhibited by rapamycin [ xref ]."
reach
"4EBP1 is phosphorylated by mTOR at serine 65 and threonine 70 XREF_BIBR."
sparser
"The interaction of 4E-BP1 with eIF4E is negatively regulated by mammalian target of rapamycin (mTORC1)-dependent phosphorylation of serine and threonine residues on 4E-BP1."
reach
"mTOR phosphorylated PHAS-I on serine and threonine residues in vitro, and these modifications inhibited the binding of PHAS-I to eIF-4E."
rlimsp
"mTOR phosphorylated PHAS-I on serine and threonine residues in vitro, and these modifications inhibited the binding of PHAS-I to eIF-4E."
sparser
"mTOR phosphorylated PHAS-I on serine and threonine residues in vitro, and these modifications inhibited the binding of PHAS-I to eIF-4E. These studies define a role for mTOR in translational control and offer further insights into the mechanism whereby rapamycin inhibits G1-phase progression in mammalian cells."
reach
"4EBP1 is phosphorylated by mTOR at serine 65 and threonine 70."
reach
"It was shown that mTOR phosphorylated 4E-BP1 at serine and threonine residues in insulin stimulated human embryonic kidney cells and these phosphorylations are inhibited by rapamycin [XREF_BIBR]."
MTOR phosphorylates EIF4EBP1 on S83. 6 / 7
1 4 | 1
hprd
No evidence text available
hprd
No evidence text available
sparser
"mTAb1-activated mTOR phosphorylated Thr36, Thr45, Ser64, Thr69, and Ser82 in PHAS-I. All five of these sites fit a (Ser/Thr)-Pro motif and are dephosphorylated in response to rapamycin in rat adipocytes."
signor
"MTOR phosphorylated PHAS-I on serine and threonine residues in vitro, and these modifications inhibited the binding of PHAS-I to eIF-4E."
hprd
No evidence text available
hprd
No evidence text available
Phosphorylated MTOR phosphorylates EIF4EBP1. 4 / 4
| 2 2
sparser
"In general, p-mTOR induces phosphorylation of 4E-BP1 and P70S6K, and initiation of translation and protein synthesis."
reach
"Given the quantitative downregulation of p-mTOR at Ser2448, this finding is sensible as p-mTOR phosphorylates 4EBP1 to allow eventual translation initiation."
sparser
"Phosphorylation of 4E-BP1 by p-mTOR releases 4E-BP1 from the mRNA cap-binding protein eIF4E, triggering the process of translation and protein synthesis [ xref , xref ]."
reach
"In general, p-mTOR induces phosphorylation of 4E-BP1 and P70S6K, and initiation of translation and protein synthesis."
MTOR phosphorylates EIF4EBP1 at position 65. 3 / 3
1 | 2
biopax:inoh
No evidence text available
reach
"4EBP1 is phosphorylated by mTOR at serine 65 and threonine 70."
reach
"4EBP1 is phosphorylated by mTOR at serine 65 and threonine 70 XREF_BIBR."
MTOR phosphorylates EIF4EBP1 at position 46. 2 / 2
1 | 1
biopax:inoh
No evidence text available
reach
"Interestingly, there is evidence that mTOR directly phosphorylates 4E-BP1 at threonine 37/46."
MTOR phosphorylates EIF4EBP1 at position 37. 2 / 2
1 | 1
biopax:inoh
No evidence text available
reach
"Interestingly, there is evidence that mTOR directly phosphorylates 4E-BP1 at threonine 37/46."
Kinase-active MTOR phosphorylates EIF4EBP1 on S65. 2 / 2
2 |
bel
"12912989;17081983"
bel
"The immunosuppressant rapamycin specifically inhibits mTOR activity and retards cancer growth. mTOR phosphorylates 4E-BP1 primarily at residues Ser65 abd Thr73. As a result, eIF4E is released from 4E-BP1 with a subsequent increase in cap-dependent translation (Mamane et al, 2004)."
Modified MTOR leads to the phosphorylation of EIF4EBP1. 2 / 2
| 2
reach
"Importantly, expression of the mTOR AAA mutant in cells activated by amino acids severely diminished the phosphorylation of S6K and 4EBP1 (XREF_FIG) as well as the phosphorylation of a transfected S6K reporter construct (XREF_SUPPLEMENTARY)."
reach
"Silencing Raptor or mTOR expression by RNAi equally decreases phosphorylation of S6K and 4EBP1, the direct downstream targets of mTORC1 XREF_BIBR."
MTOR phosphorylates EIF4EBP1 at position 70. 2 / 2
1 | 1
biopax:inoh
No evidence text available
reach
"4EBP1 is phosphorylated by mTOR at serine 65 and threonine 70."
Kinase-active MTOR phosphorylates EIF4EBP1 on T46. 2 / 2
2 |
bel
"The rapamycin-sensitive component in the 4E-BP1 phosphorylation pathway is FRAP/mTOR (FKBP12-rapamycin associated protein/mammalian target of rapamycin), also known as RAFT1 (rapamycin and 12-kD FK506 binding protein target 1), a member of the PIK (phosphoinositide kinase-related) family of kinases. (from full text) A recombinant FRAP/mTOR protein and a FRAP/mTOR immunoprecipitate were utilized in in vitro kinase assays to phosphorylate 4E-BP1. Phosphopeptide mapping of the in vitro-labeled protein yielded two 4E-BP1 phosphopeptides that comigrated with phosphopeptides produced in vivo. Mass spectrometry analysis indicated that these peptides contain phosphorylated Thr-37 and Thr-46. Thr-37 and Thr-46 are efficiently phosphorylated in vitro by FRAP/mTOR when 4E-BP1 is bound to eIF4E."
bel
"PhosphoElm data from PMID 15212693"
MTOR phosphorylates EIF4EBP1 at position 83. 1 / 1
1 |
biopax:inoh
No evidence text available
MTOR phosphorylates EIF4EBP1 on S44. 1 / 1
1 |
biopax:phosphositeplus
No evidence text available
MTOR phosphorylates EIF4EBP1 on T41. 1 / 1
1 |
biopax:phosphositeplus
No evidence text available
Kinase-active MTOR leads to the phosphorylation of EIF4EBP1 on T70. 1 / 1
1 |
bel
"From full text....In vitro, immunoprecipitated mTOR can phosphorylate 4E-BP1 at Thr36, Thr45, Thr69, Ser64, and Ser82, albeit with low and differing efficiencies (12, 20, 21)."
Kinase-active MTOR phosphorylates EIF4EBP1 on S83. 1 / 1
1 |
bel
"PhosphoElm data from PMID 15212693"
MTOR phosphorylates EIF4EBP1 on S6. 1 / 1
| 1
reach
"mTOR complex 1 (mTORC1), regulated by TSC2, phosphorylates ribosomal S6 kinase 1 (S6K1) and eukaryotic initiation factor 4E binding protein 1 (4E-BP1)."
Kinase-active MTOR phosphorylates EIF4EBP1 on S101. 1 / 1
1 |
bel
"PhosphoElm data from PMID 15212693"
MTOR leads to the phosphorylation of mutated EIF4EBP1. 1 / 1
| 1
reach
"mTORi treatment or conditional expression of a mutant 4E-BP1 that can not be phosphorylated by mTOR was sufficient to disrupt the translation initiation complex and prevent tumor growth."
Active MTOR phosphorylates EIF4EBP1. 1 / 1
| 1
trips
"PRL-activated mTOR may phosphorylate p70S6K and 4E-BP1 by restraining PP2A."