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phosphosite cbn pc11 biopax bel_lc signor biogrid tas lincs_drug hprd trrust | geneways tees isi trips rlimsp medscan sparser reach
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MTOR phosphorylates RPS6KB1. 10 / 395
1 | 1 33 128 220
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"After processing a cell proliferative up-stream signal mediated by the PI3K and Akt pathway, mTOR phosphorylates and activates p70S6K."
sparser
"Phosphorylation of p70S6K by mTOR leads to phosphorylation of insulin receptor substrate-1 (IRS-1) and its degradation via the proteasome [ xref ]."
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"Some studies suggest that mTOR can directly phosphorylate its effectors, such as S6K1 and 4E-BP1, while others claim that the mTOR dependent phosphorylation of S6K1 and 4E-BP1 is an indirect result of the inhibition of PP2A [XREF_BIBR; XREF_BIBR]."
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"Phosphorylation of S6K1 by mTOR in the heart is mediated by the mTOR complex 1 (mTORC1), which is sensitive to rapamycin."
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"5 Although rapamycin rapidly inhibits phosphorylation of p70S6K by mTOR, 4E-BP1 phosphorylation slowly decreases within 2h."
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"Under certain conditions, including nutrient starvation or pharmacological treatment, mTOR may be inhibited, resulting in dephosphorylation of p70S6K, activation of ULK1 and initiation of autophagy."
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"Global protein translation is generally suppressed under hypoxic conditions, and in our study, p70 S6 kinase, which regulates global translation and can be phosphorylated by mammalian target of rapamycin (mTOR), was inactivated in hypoxia (data not shown)."
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"42 However, inhibition of mTOR by some chemicals, such as rapa, will promote AKT phosphorylation by blocking the phosphorylation of insulin receptor substrate 1 and p70 ribosoma S6 kinase, XREF_BIBR, XREF_BIBR which are known to be a part of a negative feedback mechanism of PI3K-AKT signal transduction."
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"In regards to 4EBP1, inhibition of mTOR signaling by small interfering RNA (siRNA) inhibits phosphorylation of both p70S6K and 4EBP1 that results in apoptosis [XREF_BIBR]."
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"In mTORC1, mTOR binds to Raptor, mLST8 and PRAS40, and phosphorylates the translational factors S6K-1 and 4E-BP1 [XREF_BIBR], regulating different cell processes in response to nutrients and/or growth factors [XREF_BIBR]."
MTOR phosphorylates RPS6KB1 on T412. 10 / 57
2 5 1 | 10 16 21
rlimsp
"mTOR phosphorylates and activates p70S6K at threonine389 that serves as a marker of mTOR activity [56]."
hprd
No evidence text available
reach
"ERK phostorylates p70S6K at Thr 421 / Ser 424, while mTOR phosphorylates p70S6K at Thr 389."
rlimsp
"In this context, activation of mTORC1 leads to increased translation and cell growth by two mechanisms: first, phosphorylation of Thr389 of S6K1 by mTOR results in activation of S6K1 and subsequent phosphorylation of five evolutionarily conserved residues (Ser235, Ser236, Ser240, Ser244, and Ser247) of ribosomal protein S6 (RPS6) that activates RPS6."
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"mTOR phosphorylates and activates p70S6K at threonine 389 which serves as a marker of mTOR activity."
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"S6K1 short isoforms can not be phosphorylated by mTOR at Threonine 389 and by phosphoinositide dependent kinase 1 (PDK1) at Threonine 229, as they lack both phosphorylation sites."
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"A widely used method for assessing mTOR activity is by measuring the steady-state levels of downstream targets directly phosphorylated by mTOR, such as p70S6K phosphorylated by mTOR at threonine 389, and 4EBP1 phosphorylated by mTOR at threonine 37/46."
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"mTOR phosphorylates and activates p70S6K at threonine 389 that serves as a marker of mTOR activity XREF_BIBR."
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"mTOR phosphorylates S6K1 at T389 to enhance S6K1 activity, which amongst other things phosphorylates the S6 subunit of the ribosome to promote translation."
sparser
"Furthermore, we found that the overexpression of LPAAT-theta can induce mTOR-dependent p70S6K phosphorylation on Thr389 and 4EBP1 phosphorylation on Ser65 in HEK293T cells."
MTOR phosphorylates RPS6KB1-S6K. 10 / 10
| 10
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"Phosphorylation of p70 S6K is mediated by mTOR, and thus can be used to validate mTOR activation."
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"Furthermore, mTOR phosphorylates p70 S6K to stimulate translation of 5 ' terminal oligopyrmidine tract (5 ' TOP) mRNAs that encode ribosomes and translation factors, thus increasing the translational capacity of the cell 23."
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"RAFT1 phosphorylates p70 (S6k) much more effectively than 4E-BP1, and the phosphorylation sites on the two proteins show little homology."
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"Under high nutrient availability, mTOR is stimulated and promotes eIF4E binding protein (4E-BP1) and p70 S6K phosphorylation to activate translation initiation."
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"Here, we show that RAFT1 directly phosphorylates p70 (S6k), 4E-BP1, and 4E-BP2 and that serum stimulates RAFT1 kinase activity with kinetics similar to those of p70 (S6k) and 4E-BP1 phosphorylation."
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"Our studies and those of others on the molecular mechanisms underlying the observed sensitization to apoptosis revealed an inhibition of both mTOR- and MEK and ERK mediated phosphorylation of the kinase p70 S6K at required sites for activation, as well as down-regulation of the cell cycle regulated proteins cyclin D2 and D3."
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"Activation of mTOR increases phosphorylation of p70 S6K, which activates ribosomal protein S6 (rpS6), a substrate of p70 S6K."
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"In hippo campal neurons, increased intracellular Ca 2+ levels through intracellular stores increased mTOR dependent phosphorylation of p70 S6K, linking intracellular Ca 2+ levels with mTOR activation [XREF_BIBR, XREF_BIBR], and suggests that in these neurons, lithium will lead to an mTOR dependent activation of macroautophagy."
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"FRAP also is shown to phosphorylate PP2A in vitro, consistent with a model in which phosphorylation of PP2A by FRAP prevents the dephosphorylation of 4E-BP1 and p70 (s6k), whereas amino acid deprivation or rapamycin treatment inhibits FRAP 's ability to restrain the phosphatase."
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"Finally, it has been demonstrated that the activation of mTOR via the overexpression of Rheb, a direct activator of mTOR, is sufficient to induce an increase in p70 S6k phosphorylation, cap dependent translation and protein synthesis in skeletal muscles with normal activity."
Phosphorylated MTOR phosphorylates RPS6KB1. 8 / 8
| 1 7
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"In the downstream mTOR signaling pathways, p-mTOR can improve the translation efficiency of 5 '-TOR mRNA and accelerate protein synthesis by phosphorylating its downstream receptors during the translation process, such as elF4E and p70S6 kinase [XREF_BIBR]."
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"mTOR phosphorylation led to the phosphorylation of p70S6K, mTOR dependent event, that was abrogated by the mTOR specific inhibitor rapamycin."
sparser
"In general, p-mTOR induces phosphorylation of 4E-BP1 and P70S6K, and initiation of translation and protein synthesis."
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"The expression of p-mTOR, mTOR mediated phosphorylation of p70 ribosomal S6 protein kinase 1 (p-S6K1), 4E binding protein 4 (p-4E-BP1) as well as p-PI3K was amplified in the dorsal horn of spinal cord of BTZ rats as compared with control rats."
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"The p-mTOR activates p70S6K, and phosphorylated p70S6K combines with translation initiation complexes, to improve the efficiency of mRNA translation [XREF_BIBR]."
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"Because of the apparently discordant results noted above related to mTOR kinase activation (e.g., no change in mTOR and 4E-BP1 phosphorylation, but increased S6K1 phosphorylation), we also assessed key up-stream regulators of mTOR activity."
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"The expression of p-mTOR, mTOR mediated phosphorylation of p70 ribosomal S6 protein kinase 1 (p-S6K1), 4E binding protein 4 (p-4E-BP1), as well as phosphatidylinositide 3-kinase (p-PI3K) pathway were amplified in cyclophosphamide rats as compared with control rats."
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"Critical components in the translational machinery, such as phosphorylated mammalian target of rapamycin (mTOR) and its downstream targets, phosphorylated eukaryotic translation initiation factor and p70 S6 kinase, were up-regulated following NO treatment, and inhibition of mTOR with rapamycin attenuated NO induced increase of cyclin D1 and ODC."
MTOR phosphorylates RPS6KB1 on S394. 7 / 7
1 2 3 | 1
hprd
No evidence text available
hprd
No evidence text available
signor
"S6K1 is a positive regulator of protein synthesis, and its activity is induced by mTOR-mediated phosphorylation."
biopax:phosphositeplus
No evidence text available
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"Inhibition of mTOR by rapamycin attenuates Ser 371 phosphorylation of p70S6 kinase."
signor
"Collectively, these results indicate that Arg, Leu, and Gln act coordinately to stimulate proliferation of pTr cells through activation of the MTOR-RPS6K-RPS6-EIF4EBP1 signal transduction pathway"
hprd
No evidence text available
Kinase-active MTOR leads to the phosphorylation of RPS6KB1. 5 / 5
5 |
bel
"Activation of mTOR by insulin results in phosphorylation of downstream targets, e.g. 4E-BP1, S6K1 and ribosomal protein S6, resulting in activation of the mRNA-binding steps in translation initiation. Activation of the phosphatidylinositol 3-kinase-mammalian target of rapamycin signalling pathway by amino acids Although it is clear that amino acids, and in particular leucine, enhance phosphorylation of proteins downstream of mTOR, e.g. 4E-BP1 and S6K1, and indeed require mTOR to be active in order to be effective, whether or not they directly regulate mTOR activity is undecided."
bel
"In addition, two proteins that interact with mTOR to target it to two important substrates, eukaryotic initiation factor 4E binding protein 1 and ribosomal protein S6 kinase, have been identified."
bel
"When part of a complex that also contains the proteins raptor and GbetaL, mTOR phosphorylates substrates like S6K1 and 4E-BP1."
bel
"once Akt1 is activated, it initiates a cascade of phosphorylation events targeting mammalian target of rapamycin, mTOR, which in turn phosphorylates the p70S6 kinase and GSK3B"
bel
"Activation of mTOR results in phosphorylation of a variety of substrates, including the eIF4E-binding protein (4E-BP1), and the ribosomal protein S6 protein kinase (S6K1; for review, see Gingras et al. 2001)."
MTOR phosphorylates RPS6KB1-S6K on T412. 4 / 4
| 4
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"RAFT1 phosphorylates p70 (S6k) on Thr 389, a residue whose phosphorylation is rapamycin sensitive in vivo and necessary for S6 kinase activity."
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"Yet, in the absence of raptor, the ability of mTOR to phosphorylate p70 s6k T389 was still sensitive to inhibition by rapamycin."
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"mTOR in turn phosphorylates p70 S6K at Thr 389."
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"Increased mTOR activity enhances Thr 389 phosphorylation of p70S6 kinase (S6K) as an indicative of activation of mTOR signaling, and leads to glomerular hypertrophy in diabetic nephropathy."
MTOR phosphorylated on S2448 phosphorylates RPS6KB1. 4 / 4
4 |
biopax:pid
No evidence text available
biopax:pid
No evidence text available
biopax:pid
No evidence text available
biopax:pid
No evidence text available
Kinase-active MTOR phosphorylates RPS6KB1 on T444. 4 / 4
2 2 |
bel
"Recently, it has been demonstrated that Thr229 phosphorylation is mediated by PDK1 (15, 16), whereas Thr389 as well as Ser411, Thr421, and Ser424 phosphorylation have been shown to be regulated by mTOR (17)."
bel
"12586835;11914378;17081983"
bel
"12586835;11914378;17081983"
bel
"Recently, it has been demonstrated that Thr229 phosphorylation is mediated by PDK1 (15, 16), whereas Thr389 as well as Ser411, Thr421, and Ser424 phosphorylation have been shown to be regulated by mTOR (17)."
MTOR phosphorylates RPS6KB1 on T390. 4 / 4
1 3 |
hprd
No evidence text available
hprd
No evidence text available
hprd
No evidence text available
signor
"Thr229 phosphorylation requires prior phosphorylation of the Ser/Thr-Pro sites in the autoinhibitory domain and Thr389 in the linker domain,[…] Moreover, in vitro mTOR directly phosphorylates Ser371, and this event modulates Thr389phosphorylation by mTOR, compatible with earlier in vivo findings."
Kinase-active MTOR phosphorylates RPS6KB1 on T412. 4 / 4
2 2 |
bel
"11914378;16099428;15809305;15718470;15388791"
bel
"Recently, it has been demonstrated that Thr229 phosphorylation is mediated by PDK1 (15, 16), whereas Thr389 as well as Ser411, Thr421, and Ser424 phosphorylation have been shown to be regulated by mTOR (17)."
bel
"11914378;16099428;15809305;15718470;15388791"
bel
"Recently, it has been demonstrated that Thr229 phosphorylation is mediated by PDK1 (15, 16), whereas Thr389 as well as Ser411, Thr421, and Ser424 phosphorylation have been shown to be regulated by mTOR (17)."
MTOR leads to the phosphorylation of RPS6KB1 on T444. 3 / 3
| 1 2
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"Furthermore, the knockdown of Mtor by Mtor siRNA reduced insulin- induced phosphorylation of RPS6KB1 Thr 389, RPS6KB1 Thr 421 / Ser 424, RPS6 Ser 235/236 and EIF4EBP1 Thr 37/46 (XREF_FIG)."
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"Inhibition of mTOR with rapamycin blocked the Thr 389 but not the Thr-421 and Ser-424 phosphorylation of S6K1."
sparser
"Ethanol inhibition of p70S6K phosphorylation could have been mediated by inhibition of Akt, mTOR, or both, as the multiplex-ELISA detected pTpS421/424 -p70S6K; the T421 and S424 residues on p70S6K are phosphorylated by Akt and mTOR ( xref )."
Kinase-active MTOR phosphorylates RPS6KB1 on serine. 3 / 3
3 |
bel
"mTOR can control the mammalian translation machinery by direct phosphorylation and activation of p70 ribosomal S6 kinase (p70rsk)57, as well as phosphorylation of the initiation factor 4E for eukaryotic translation (eIF-4E) inhibitor, PHAS1 or 4E-binding protein 1 (ref. 58)."
bel
"TOR activity leads to phosphorylation and activation of ribosomal S6 kinase-1 (S6K1), and phosphorylation and inhibition of translation initiation factor eIF-4E binding protein-1 (4E-BP1)."
bel
"mTOR stimulates protein synthesis by phosphorylating p70 S6 kinase (p70 S6K) and eIF4E binding proteins 1, 2, and 3 (4E-BPs). In turn, p70 S6K phosphorylates the ribosomal protein S6"
Kinase-active MTOR phosphorylates RPS6KB1 on S447. 3 / 3
2 1 |
bel
"12586835;11914378;17081983"
bel
"Recently, it has been demonstrated that Thr229 phosphorylation is mediated by PDK1 (15, 16), whereas Thr389 as well as Ser411, Thr421, and Ser424 phosphorylation have been shown to be regulated by mTOR (17)."
bel
"Recently, it has been demonstrated that Thr229 phosphorylation is mediated by PDK1 (15, 16), whereas Thr389 as well as Ser411, Thr421, and Ser424 phosphorylation have been shown to be regulated by mTOR (17)."
MTOR phosphorylates RPS6KB1 on S434. 3 / 3
1 2 |
signor
"Collectively, these results indicate that Arg, Leu, and Gln act coordinately to stimulate proliferation of pTr cells through activation of the MTOR-RPS6K-RPS6-EIF4EBP1 signal transduction pathway"
biopax:phosphositeplus
No evidence text available
signor
"S6K1 is a positive regulator of protein synthesis, and its activity is induced by mTOR-mediated phosphorylation."
MTOR-S2215Y phosphorylates RPS6KB1. 2 / 2
| 2
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"mTOR S2215Y also enhanced the phosphorylation of S6K1 but, in the presence of rapamycin, S6K1 phosphorylation was completely abolished."
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"We next examined whether rapamycin, an mTOR inhibitor, inhibits the enhanced phosphorylation of S6K1 by mTOR S2215Y."
MTOR leads to the phosphorylation of RPS6KB1 on S447. 2 / 2
| 1 1
sparser
"Ethanol inhibition of p70S6K phosphorylation could have been mediated by inhibition of Akt, mTOR, or both, as the multiplex-ELISA detected pTpS421/424 -p70S6K; the T421 and S424 residues on p70S6K are phosphorylated by Akt and mTOR ( xref )."
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"Inhibition of mTOR with rapamycin blocked the Thr 389 but not the Thr-421 and Ser-424 phosphorylation of S6K1."
Modified MTOR leads to the phosphorylation of RPS6KB1. 2 / 2
| 2
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"We found that ectopic expression of constitutively active mTOR increased the basal level of phosphorylation of S6K1, but not Akt, in serum starved Rh30 cells (XREF_FIG), suggesting that the constitutively active mTOR was functional in the cells."
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"To this end, we found that curcumin reduced expression of mTOR, Raptor and Rictor protein and mRNA levels, as well as downstream mTORC1 mediated phosphorylation of p70S6K and 4E-BP1."
Kinase-active MTOR phosphorylates RPS6KB1 on S434. 2 / 2
2 |
bel
"9271440;12586835;11914378;14993219;15208671;15809305"
bel
"Recently, it has been demonstrated that Thr229 phosphorylation is mediated by PDK1 (15, 16), whereas Thr389 as well as Ser411, Thr421, and Ser424 phosphorylation have been shown to be regulated by mTOR (17)."
MTOR phosphorylates RPS6KB1 on serine. 1 / 1
| 1
rlimsp
"S104/106 are proline-directed sites, while S118 resides within a sequence that resembles a hydrophobic motif, which are phosphorylated by mTOR in its substrates 4E-BP1 and S6K1, respectively (Reviewed in 34, 35)."
Kinase-active MTOR phosphorylates RPS6KB1 on T390. 1 / 1
1 |
bel
"9271440;12370290;9465032;11914378;14560963"
MTOR leads to the phosphorylation of RPS6KB1 on S40. 1 / 1
| 1
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"mTOR promotes translation initiation by phosphorylating p70S6K kinase and 40S ribosomal protein S6 (Alam et al, 2004)."
MTOR phosphorylates RPS6KB1 at position 389. 1 / 1
1 |
biopax:inoh
No evidence text available
MTOR leads to the phosphorylation of mutated RPS6KB1. 1 / 1
| 1
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"Here, we demonstrate that the raptor and mTOR complex phosphorylates the rapamycin sensitive forms of S6K1, while the distinct rictor and mTOR complex phosphorylates the rapamycin resistant mutants of S6K1."
MTOR phosphorylates RPS6KB1 on threonine. 1 / 1
| 1
rlimsp
"At a molecular level, this dual control of p70 S6K activity is attributable to phosphorylation of the two p70 S6K sites: The Ptd Ins 3,4,5P3-dependent kinasel (PDK1) phosphorylates p70 S6K at a Thr on the activation loop, whereas mTOR phosphorylates a Thr located in a hydrophobic motif carboxyterminal to the catalytic domain."
Kinase-active MTOR phosphorylates RPS6KB1 on S394. 1 / 1
1 |
bel
"12586835;11914378;14993219"
MTOR phosphorylates RPS6KB1 on T252. 1 / 1
| 1
reach
"S6K1 short isoforms can not be phosphorylated by mTOR at Threonine 389 and by phosphoinositide dependent kinase 1 (PDK1) at Threonine 229, as they lack both phosphorylation sites."