A database built with INDRA combining content from numerous readers and databases. This page allows you to curate the loaded statements. For more information please see the manual.

IndraLab

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phosphosite cbn pc11 biopax bel_lc signor biogrid tas lincs_drug hprd trrust | geneways tees isi trips rlimsp medscan sparser reach
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AKT1 phosphorylates MTOR. 10 / 16
2 1 | 2 3 8
biopax:panther
No evidence text available
sparser
"Interestingly, we found the active form of MTOR, phospho-(Ser2448)-MTOR, at the base of primary cilia in wild-type MEFs ( xref ) making it likely that phospho-(Ser473)-AKT1 phosphorylates MTOR at primary cilia."
reach
"Furthermore, upstream overexpression or knockdown of Akt2 or Akt2 phosphorylation inhibition, rather than Akt1 or Akt3, resulted in striking hyperphosphorylation or dephosphorylation of mTOR, p70S6K and rpS6 (all P < 0.05), without any change in total proteins expressions."
reach
"This leads to the activation of protein kinase B (Akt) which then phosphorylates the mechanistic target of rapamycin (mTor) [XREF_BIBR, XREF_BIBR]."
signor
"Once phosphorylated, Akt can act on a broad spectrum of substrates that can influence cell survival and proliferation and protein synthesis (65). Phosphorylation of mTOR by Akt leads to mTOR activation (40, 52) and the subsequent activation of p70S6K"
reach
"In Figures XREF_FIG, application of the phosphoinositide 3-kinase (PI 3-K) inhibitor LY294002 (10 muM) during treatment with either EPO (10 ng/ml) or Wnt1 (100 ng/ml) prevented EPO or Wnt1 from phosphorylating mTOR and p70S6K, illustrating that the PI 3-K and Akt1 pathways are necessary for EPO or Wnt1 to phosphorylate mTOR and p70S6K."
sparser
"Akt1 can phosphorylate mTOR ( xref ), which can then in turn trigger phosphorylation of S6 protein for its activation."
reach
"We have investigated the effects of insulin, amino acids, and the degree of muscle loading on the phosphorylation of Ser (2448), a site in the mammalian target of rapamycin (mTOR) phosphorylated by protein kinase B (PKB) in vitro."
reach
"Despite modulation of PRAS40, which regulates the mTOR containing TORC1 complex, mutational activation of PIK3CA or AKT1 did not consistently increase phosphorylation of mTOR at serine 2448 or phosphorylation of mTOR target proteins and their targets, including p70-ribosomal protein S6-kinase (p70S6K), eukaryotic elongation factor 4 binding protein 1 (EIF4EBP1), and ribosomal protein S6."
sparser
"We speculated that RhebL1 might bind to AKT1 directly or indirectly via mTOR since RhebL1 could bind to mTOR and mTOR was phosphorylated by AKT1 [ xref , xref ]."
AKT1 phosphorylates MTOR on S2448. 10 / 10
1 1 3 | 3 2
reach
"mTOR is phosphorylated by Akt1 at Ser2448, leading to downstream signaling by p-mTOR."
sparser
"Activated mTOR is phosphorylated at Ser 2448 by AKT1 and functions in mediating cell growth and proliferation [16] ."
hprd
No evidence text available
sparser
"The mTORC1 is acutely inhibited by rapamycin, which has been used therapeutically and experimentally as a probe to gain insight into mTORC1 regulation and function. xref The mTORC2 is significantly less sensitive to rapamycin than mTORC1. xref mTORC1 is known to suppress autophagy, which is a key catabolic process. xref The mTOR is phosphorylated by Akt1 at Ser2448 to yield phospho-mTOR (p-mTOR), which regulates ribosome biogenesis and protein synthesis, xref and is also present in the cytoplasm and nucleus, with high p-mTOR expression indicating reduced response to tumor treatment. xref , xref "
reach
"Despite modulation of PRAS40, which regulates the mTOR containing TORC1 complex, mutational activation of PIK3CA or AKT1 did not consistently increase phosphorylation of mTOR at serine 2448 or phosphorylation of mTOR target proteins and their targets, including p70-ribosomal protein S6-kinase (p70S6K), eukaryotic elongation factor 4 binding protein 1 (EIF4EBP1), and ribosomal protein S6."
biopax:phosphositeplus
No evidence text available
sparser
"mTOR is phosphorylated by Akt1 at Ser2448, leading to downstream signaling by p-mTOR. xref The premise of our study was to examine the regulatory relationship between the mTOR pathway and PKM2."
hprd
No evidence text available
hprd
No evidence text available
signor
"Although AKT phosphorylated mTOR at two COOH-terminal sites (Thr2446 and Ser2448) in vitro, Ser2448 was the major phosphorylation site in insulin-stimulated or -activated AKT-expressing human embryonic kidney cells. Transient transfection assays with mTOR mutants bearing Ala substitutions at Ser2448 and/or Thr2446 indicated that AKT-dependent mTOR phosphorylation was not essential for either PHAS-I phosphorylation or p70S6K activation in HEK cells."
AKT1 phosphorylates MTOR on T2446. 4 / 4
1 1 2 |
signor
"AKT phosphorylated mTOR at two COOH-terminal sites (Thr2446 and Ser2448) in vitro, Ser2448 was the major phosphorylation site in insulin-stimulated or -activated AKT-expressing human embryonic kidney cells. These results demonstrate that mTOR is a direct target of the PI3K-AKT signaling pathway in mitogen-stimulated cells, and that the identified AKT phosphorylation sites are nested within a repressor domain that negatively regulates the catalytic activity of mTOR."
biopax:phosphositeplus
No evidence text available
hprd
No evidence text available
hprd
No evidence text available
AKT1 phosphorylated on S473 and T308 phosphorylates MTOR on S2448. 2 / 2
2 |
biopax:pid
No evidence text available
biopax:pid
No evidence text available
Modified AKT1 leads to the phosphorylation of MTOR. 2 / 2
| 2
reach
"In this study, we constructed lentiviral vectors expressing constitutively active Akt1 and Akt3, two major isoforms of Akt, which reduced brain infarction in rats We found that overexpression of Akt1 and Akt3 not only promoted protein phosphorylation in the Akt pathway, including p-Akt, p-PTEN, and p-FKHR, but also promoted mTOR phosphorylation in the mTOR pathway."
reach
"Overexpression of Akt1 upregulates glycogen synthase activity and phosphorylation of mTOR in IRS-1 knockdown HepG2 cells."
Kinase-active AKT1 phosphorylates MTOR. 1 / 1
1 |
bel
"once Akt1 is activated, it initiates a cascade of phosphorylation events targeting mammalian target of rapamycin, mTOR, which in turn phosphorylates the p70S6 kinase and GSK3B"
Phosphorylated AKT1 phosphorylates MTOR. 1 / 1
| 1
reach
"In the OHCM group, there was upregulation of activators of insulin signaling including phosphorylated adenosine monophosphate activated protein kinase alpha, protein kinase B, phosphorylated protein kinase B, mammalian target of rapamycin, phosphorylated mammalian target of rapamycin, and phosphoinostitide 3-kinase, and upregulation of inhibitors including phosphorylated insulin receptor substrate 1, phosphorylated insulin receptor substrate 2, and retinol binding protein 4."
Kinase-active AKT1 leads to the phosphorylation of MTOR on S2448. 1 / 1
1 |
bel
"In contrast, HepG2-CA-Akt/PKB cells showed an upregulation in the basal level of phosphorylated mTOR (Ser 2448) (ca. 40%)"