IndraLab

"FoxH1 Represses Both Ligand-dependent and -independent Transactivation of the AR in LNCaP Cells—Next, we examined the possible roles of FoxH1 expression in ligand-dependent and -independent transcription of the PSA promoter induced by endogenous AR by cotransfecting LNCaP cells, the most commonly used androgen-sensitive prostate cancer cell line, with a FoxH1 expression plasmid and a PSA-luciferase reporter gene. As shown in Fig. 1B, DHT activated the AR in a concentration-dependent manner, and an ~10-fold higher induction was observed in the presence of 1 µM DHT compared with vehicle treatment. Cotransfection of the FoxH1 expression plasmid brought about a marked repression of the DHT-induced AR activation at all concentrations of DHT tested (Fig. 1B), and the repression was dose-dependent (Fig. 1C). "