IndraLab

"In addition, depletion of RhoG significantly inhibits EGF stimulated Rac1 activation in these cells."

"A second wave of EGF-induced Rac1 activation was required for EGF-induced cell migration, however, the spatiotemporal regulation of the second wave of EGF-induced Rac1 activation remains largely unclear."

"In contrast, combined Cbl and Cbl-b KD dramatically enhanced the EGF-induced Vav2 phosphorylation in response to EGF stimulation ( xref G ) and enhanced the EGF-induced Rac1 and Cdc42 activation ( xref , A and B )."

"A second wave of EGF induced Rac1 activation was required for EGF induced cell migration, however, the spatiotemporal regulation of the second wave of EGF induced Rac1 activation remains largely unclear."

"Tiam1 expression is required for the second wave of EGF-induced Rac1 activation in A431 cells."

"Both EGF and HGF can induce several Rac1 or Cdc42 specific Guanine nucleotide exchange factors (GEFs) [XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR, XREF_BIBR]; however, it is still unclear which RacGEF (s) are responsible for MLK3 activation."

"Additionally, we showed that Rac1 activation by EGF was prevented by addition of an Akt inhibitor (Figure xref )."

"We also showed that the extent of EGF induced Rac1 activation in MDCK and CIIA-Flag cells was markedly greater than that in MDCK and control cells (XREF_FIG)."

"Indeed, this concept is supported by a previous study showing that EGF treatment of MCF-7 and MDA-MB-231 breast cancer cells promotes the release and activation of RhoA, Rac1 and Cdc42 by Ser174 phosp[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"

"Epithelial growth factor (EGF)-mediated activation of Rho GTPase Rac1 and formation of lamellipodia in TS/A cells were remarkably suppressed by DET treatment."

"In the present study the function of Ash/Grb-2 was investigated by microinjecting cells with an anti-Ash antibody. The antibody abolished both S phase entry and the reorganization of actin assembly to ruffle formation upon stimulation with epidermal growth factor (EGF) and platelet-derived growth factor (PDGF). On the other hand, anti-Ash antibody had no effect on S phase entry or actin stress fiber formation induced by either serum or lysophosphatidic acid. Since the induction of DNA synthesis, ruffle induction and stress fiber formation involve a function of Ras, Rac activation and Rho activation respectively, the findings strongly suggest that Ash plays a critical role in the signaling of both pathways downstream from growth factor receptors to Ras and Rac. Consistent with this, Ash co-precipitated with EGF receptor from EGF-stimulated cells."

"EGF stimulation of wild type Rac1 or Rac1 (R163A) in HeLa cells led to the activation of Rac1 (XREF_FIG)."

"Rac1 activation by EGF was completely eliminate in cells transfected with pSUPER-beta-Pix (Fig. 2C). This result demonstrates that beta-Pix is an essential GEF for Rac1. # beta-PIX = ARHGEF7"