"We determined that EGF activates phosphorylation of Akt, mTOR, p70S6 kinase, ribosomal protein S6, eIF4E and 4E-BP1 in a PI-3-kinase dependent manner, and PI-3-kinase activation may prevent lactogenic differentiation in HC11 mammary epithelial cells by regulating the synthesis of proteins."
"Indeed, western blotting analysis suggests that ouabain treatment reduced EGF-induced phosphorylation of ERK 1/2 and P70S6K (the downstream target of mTOR pathway), but not AKT or 4EBP1 ( xref )."
"In contrast, in MCF10A cells, and those expressing SCRIBWT, EGF stimulation did not significantly increase p70S6K phosphorylation up to 45 minutes after stimulation."
"EGF stimulation induced phosphorylation of p70 in HepG2 and Hep3B, and to a lesser extent in Huh7 cells."
"Also harmine treatment was able to interfere with EGF-stimulated S6K1 phosphorylation, although to a lesser degree ( Figure S3 B)."
"BI-D1870 and SL0101 showed no important change in EGF-induced phosphorylation of rpS6 and p70S6K ( Fig. 7 F and G)."
"BI-D1870 showed no important change in EGF-induced phosphorylation of rpS6 and p70S6K ( Fig. 7 F and G)."
"Mechanistically, MEL specifically inhibited the EGF induced HIF-1alpha expression by suppressing the phosphorylation of ERK, mTOR and p70S6K."
"EGF induced a rapid phosphorylation of Akt, tuberin, p70S6K and 4E-BP1 ( xref )."
"As shown in xref , EGF-induced phosphorylation of mTOR, 4E-BP1, and P70S6K was significantly decreased in SiNUAK1/H1299 cells compared with Scr/H1299 cells."