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phosphosite cbn pc11 biopax bel_lc signor biogrid tas lincs_drug hprd trrust | geneways tees isi trips rlimsp medscan sparser reach
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TP53 increases the amount of TP53. 5 / 424
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"In particular, climacostol activated p53 inducing the upregulation of p53 protein levels in the nuclei through effects on p53 stability at translational level, as for instance the phosphorylation at Ser15 site."
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"Phosphorylation of p53 by TAF1 inactivates p53 dependent transcription in the DNA damage response."
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"The results of immunohistochemical studies and RT-qPCR showed that Ad-p53 upregulated the expression of p53, and downregulated the expression of TGF-beta1 and alpha-SMA."
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"In a feedback fashion, they work together to directly inhibit the transcriptional activity of p53 and mediate p53 degradation via ubiquitin dependent proteolysis, as MDM2 possesses an E3 ubiquitin ligase activity and its mRNA expression is stimulated by p53, thus, keeping p53 level and activity marginally detectable in most of normal mammalian cells or tissues."
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"p53 gene silencing significantly suppressed p53 and p21 mRNA expression without affecting p27 mRNA expression."
Modified TP53 increases the amount of TP53. 10 / 27
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"In fact, the interaction between the two is a two way street, with several miRNAs being able to regulate p53 expression either through direct targeting, or through regulation of other proteins that in turn modulate p53 expression and function."
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"An increased level of TP53 targeting miRNAs in GC B cells such as miR-21, miR-25 and miR-200c could promote an additional control level of TP53 expression after the activation of the DNA damage response."
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"As demonstrated in XREF_FIG, irradiation of the wild-type p53 expressing HCT116 cells markedly increased p53 levels, which were also upregulated by treatment with valproic acid."
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"For example high levels of p53 expression in embryonic development causes cell death in neuronal cells and retina, moderate levels of p53 expression causes replicative senescence in hematopoietic progenitor cells, whereas low but chronic level of p53 expression causes senescence in pneumocytes and fibrosis in lung and liver tissue [XREF_BIBR]."
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"Suppression of p53 expression by siRNA inhibited the expression of p53 as well as its target genes along with the prevention of apoptosis induction."
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"Overexpression of p53 activates transcription of p53 effector genes, causes growth inhibition and induced apoptosis."
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"In contrast, the p53 antagonizing oncogene mdm2 was scarcely expressed at 0-1 h post-irradiation, but was drastically enhanced at 2-3 h post-irradiation, eventually paralleling p53 expression."
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"Basal levels of wild-type p53 can stimulate PGC-1alpha expression and mitochondrial biogenesis in mice, while severe cellular stress associated with telomere dysfunction and increased p53 levels can repress both PGC-1alpha and PGC-1beta expression [XREF_BIBR, XREF_BIBR]."
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"Presumably, the reductions in mRNA levels were caused by the decrease in expression of p53 proteins from late torpor to arousal, resulting in the restabilization of p53 downstream gene expressions."
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"Next, to investigate whether RITA induced senescence depends on p53 status, we treated the stable-p53-knockdown cell lines HN30-shp53 and HN31-shp53 with RITA and found that knockdown of p53 protein expression markedly reduced both the expression and phosphorylation of p53 (XREF_FIG)."
Mutated TP53 increases the amount of TP53. 10 / 16
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"Mutant p53 does not induce the expression of wild-type (wt) p53 downstream genes because of the loss of the ability to bind to p53 response elements [XREF_BIBR, XREF_BIBR]."
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"Dominant negative effects of mutant p53 have been shown to suppress wild-type p53 expression and function."
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"Mutations of the p53 tumor suppressor gene often result in overexpression of the p53 protein."
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"The overexpression of p53, detected by IHC and qPCR, was mainly caused by TP53 mutations, which correlates with the results obtained by Yemelyanova et al, stating that the immunohistochemical pattern of p53 in tumors is a predictor of TP53 mutations [XREF_BIBR]."
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"Transient expression of mutant p53 protein in TSGH8301 cells may induce an irreversibly stabilization of p53 and increase the steady state of p53 expression."
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"XREF_FIG demonstrates that in the total cell lysate of virtually all the lymphoma cell lines with either wild-type (wt) or mutant p53, doxorubicin treatment increased p53 protein levels starting at 3h, and the increases were sustained at 6 and 24 h (the results from DoHH2, JOK-1, and DHL-4 cells are shown; anti-p53 antibody : FL393)."
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"Tubo-ovarian and peritoneal high-grade serous carcinoma (HGSC) is characterized by aberrant p53 expression induced by a TP53 mutation."
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"Treatment with poly (I : C) had no impact on WT or p53 mutant mediated expression of the p53 target genes p21 and PUMA (BBC3) (XREF_SUPPLEMENTARY)."
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"This result indicates that (1) the abnormal p53 expression is usually not caused by mutations of the p53 gene in PPC and (2) PPC is part of a growing number of tumors that share evidence of p53 dysfunction in the absence of mutation."
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"We thus asked whether mutant p53 would be able to modulate the expression of classical wild-type p53 responsive target genes."
TP53 increases the amount of mutated TP53. 6 / 6
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"It is important to note that no prior study has linked p53 or p73 to the mechanism of action of either prodigiosin or its related structures and no prior study has demonstrated the ability of these compounds to restore p53 pathway signaling in mutant p53 expressing or p53-null cancer cells."
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"These results suggest that NSC59984 restores p53 pathway signaling in mutant p53 expressing SW480 and DLD-1 human colorectal cancer cells."
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"MKP-1 has been shown to be transcriptionally regulated by p53, and mutations of p53 abrogate p53 dependent transcription of MKP-1 in vitro (Yang and Wu, 2004; Liu et al, 2008)."
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"NSC59984 specifically restores p53 pathway signaling in mutant p53 expressing human colorectal cancer cells."
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"XREF_BIBR In addition to CDKN1A and p21 CIP1, other traditional p53 targets, such as GADD45 and 14-3-3sigma, also were up-regulated but perhaps not as a result of p53 activation given that 779e cells express no detectable p53 protein and that E47 repressed the expression of mutant p53 mRNA and protein in the other 4 lines (XREF_FIG A-F)."
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"Thus we determined the need to isolate single cell DLD1 clones with p53-EGFP reporter expression that have fairly low basal EGFP expression and that could be induced to high EGFP expression upon exposure to small molecules that restore p53 pathway signaling in mutant p53 expressing DLD1 cells."
Mutated TP53 increases the amount of mutated TP53. 4 / 4
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"As compared with EV transfected cells, let-7i promoter activity was significantly reduced by exogenous mutant p53 (XREF_FIG), suggesting that mutant p53 represses let-7i transcription."
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"TP53 mutations are highly frequent in cancer and lead to the expression of mutant p53 proteins that are endowed with oncogenic activities and sensitive to stress signaling."
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"Several studies documented the importance of either wild-type or mutant p53 alleles in leading to increased expression of p53 protein increased expression of both wild-type and mutant p53 protein."
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"Despite the partial or complete loss of sequence specific DNA binding, mutant p53 proteins can induce or repress transcription of mutant p53 specific target genes and various mutant p53 proteins can bind to oligonucleotides mimicking non B DNA structures [XREF_BIBR]."
Phosphorylated TP53 increases the amount of TP53. 4 / 4
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"In response to stress and DNA damage, p53 is phosphorylated on multiple serine residues, including Ser 15, resulting in inhibition of degradation and increased p53 levels."
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"Cellular stresses such as irradiation, lead to phosphorylation and dissociation of p53 and MDM2, which allow p53 protein levels to increase."
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"1 For example, phosphorylation and acetylation of p53 have been shown to promote the expression of p53 transcriptional targets, whereas other modifications, such as ubiquitination, sumoylation, and neddylation have been associated with the suppression of p53 mediated transcription and p53 nuclear export."
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"The Chk signal transducers induce cell cycle arrest by phosphorylation of two critical effectors : inhibitory phosphorylation of the Cdc25 phosphatase results in CDK inactivation, while the activating phosphorylation of the p53 tumor suppressor stabilizes p53 and increases transcription of p53 target genes, including the cyclin dependent kinase inhibitor p21/CIP1/WAF-1."
TP53 bound to HTT increases the amount of TP53. 3 / 3
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"Mutant huntingtin interacts with p53, a transcription factor induced in response to DNA damage, and increases the levels and activity of p53 in mouse striatal cells, and in striatum and cerebral cortex from HD patients and R6/2 transgenic mice [XREF_BIBR, XREF_BIBR]."
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"Mutant huntingtin (mHtt) with expanded polyglutamine (polyQ) binds to p53 and upregulates levels of nuclear p53 as well as p53 transcriptional activity in neuronal cultures."
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"Mutant huntingtin binds to p53 and upregulates the levels of nuclear p53 as well as p53 transcriptional activity in neuronal cultures."
Acetylated TP53 increases the amount of TP53. 2 / 2
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"Based on results showing that either ubiquitinated or acetylated p53 is competent to activate transcription of p53 target genes, an activity which requires p53 tetramer formation, we chose to pursue an investigation of the latter hypothesis XREF_BIBR, XREF_BIBR."
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"Subsequent assays indicated gamma-bisabolene eliciting p53 acetylation that enhanced the expression of p53 regulated apoptotic genes."
Sumoylated TP53 increases the amount of TP53. 2 / 2
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"On the other hand, sumoylation of DJ-1 and p53 reactivates p53 transcription through a Topors and p53BPs complex [XREF_BIBR]."
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"Since p53 SUMOylation can increase p53 expression by decreasing its degradation, it is also possible that p53 SUMOylation can increase EC inflammation via regulating KLF2 expression."
TP53 increases the amount of TP53-Y220C. 1 / 1
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"To further address this issue, we also performed the p53 transcriptional reporter assay in Hep3B cells and found that the Y220C mutant of p53 did not activate p53 dependent transcription (XREF_FIG)."
Modified mutated TP53 increases the amount of TP53. 1 / 1
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"We report here that down-regulation of mutant p53 expression in several human cancer cell lines harbouring mutant p53 by siRNA mediated silencing induced the expression of p53 dependent apoptotic and repair genes."
TP53 bound to SOCS3 increases the amount of TP53. 1 / 1
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"Immunoprecipitation analyses revealed that SOCS3 bound to p53 and subsequently increased the expression of p53 and its target genes, contributing to IL-22-mediated HSCs senescence [XREF_BIBR]."
TP53 phosphorylated on amino acid 15 increases the amount of TP53. 1 / 1
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"We measured nuclear phospho-p53 Ser15 levels after HI by Western blot analysis because phosphorylation of p53 at serine 15 activates p53 mediated proapoptotic transcription."
TP53 phosphorylated on S6 increases the amount of TP53. 1 / 1
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"For example, JNK can phosphorylate p53 at Ser6, which inhibits ubiquitin mediated p53 degradation and increases the p53 expression level, resulting in p53 mediated up-regulation of several pro apoptotic genes, including Bax and PUMA [XREF_BIBR, XREF_BIBR]."
TP53-R280T increases the amount of TP53. 1 / 1
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"Although there was no significant difference in expression of CDKN1A, VDR, and TP53INP1 between CD24 + PC3 cells and CD24 - PC3 cells, the transfections of WT TP53, mutants TP53 R273H, TP53 V143A, and TP53 R280T, but not mutant TP53 R175H, induced the expression of these p53 target genes in CD24 - PC3 cells but not in CD24 + PC3 cells (XREF_FIG)."
TP53-R273H increases the amount of TP53. 1 / 1
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"Although there was no significant difference in expression of CDKN1A, VDR, and TP53INP1 between CD24 + PC3 cells and CD24 - PC3 cells, the transfections of WT TP53, mutants TP53 R273H, TP53 V143A, and TP53 R280T, but not mutant TP53 R175H, induced the expression of these p53 target genes in CD24 - PC3 cells but not in CD24 + PC3 cells (XREF_FIG)."
TP53 increases the amount of TP53-R270H. 1 / 1
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"We generated female p53 (lox and lox)/FAK (+/+)/WapCre, p53 (lox and lox)/FAK (flox/+)/WapCre and p53 (lox and lox)/FAK (flox/-)/WapCre mice, and mice with WapCre mediated conditional expression of p53 (R270H), the mouse equivalent of human p53 (R273H) hot spot mutation, together with conditional deletion of FAK, P53 (R270H/+)/FAK (lox/+)/WapCre and p53 (R270H/+)/FAK (flox/-)/WapCre mice."
TP53 bound to DAXX increases the amount of TP53. 1 / 1
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"However, another group suggested that interaction between DAXX and wild-type p53 modulates expression of p53 target genes differentially, for example promoting the activation of Puma and inhibition of p21 and Mdm2."
TP53 phosphorylated on S15 increases the amount of TP53. 1 / 1
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"ultimate carcinogen benzo[a]pyrene diol epoxide (BPDE) Analyses of proteins in treated cells indicated that p53 was phosphorylated at Ser15 but not at Ser20 within 30 min of treatment, and this correlated with an increase in the total amount of p53 protein."
TP53 bound to TOP3A increases the amount of TP53. 1 / 1
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"The binding of TOP3A to both p53 and p21 promoter regions promoted the expression of p53 and p21, and mediated tumor suppression in a p53 dependent manner [XREF_BIBR]."
Methylated TP53 increases the amount of TP53. 1 / 1
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"These near-polyploid cells overexpressed DNA repair genes to reduce the DNA-damage response, as well as methylating p53 promoter regions to silence p53 expression."
Ubiquitinated TP53 increases the amount of TP53. 1 / 1
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"Together, these results indicate that Hoxa2 negatively affects RCHY1 stability and RCHY1 dependent ubiquitination of p53 thereby inducing a stabilization of p53 and an increase in p53 protein level."
TP53 bound to PTEN increases the amount of TP53. 1 / 1
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"Also, PTEN Akt-independently interacts with p53 and cyclin D, and stabilizes p53, whose half-life is short in the nucleus, and promotes transcription of p53 XREF_BIBR XREF_BIBR."
TP53-V143A increases the amount of TP53. 1 / 1
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"Although there was no significant difference in expression of CDKN1A, VDR, and TP53INP1 between CD24 + PC3 cells and CD24 - PC3 cells, the transfections of WT TP53, mutants TP53 R273H, TP53 V143A, and TP53 R280T, but not mutant TP53 R175H, induced the expression of these p53 target genes in CD24 - PC3 cells but not in CD24 + PC3 cells (XREF_FIG)."
TP53 increases the amount of sumoylated TP53. 1 / 1
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"On the other hand, sumoylation of DJ-1 and p53 reactivates p53 transcription through a Topors and p53BPs complex [XREF_BIBR]."
TP53-R175H increases the amount of TP53. 1 / 1
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"Although there was no significant difference in expression of CDKN1A, VDR, and TP53INP1 between CD24 + PC3 cells and CD24 - PC3 cells, the transfections of WT TP53, mutants TP53 R273H, TP53 V143A, and TP53 R280T, but not mutant TP53 R175H, induced the expression of these p53 target genes in CD24 - PC3 cells but not in CD24 + PC3 cells (XREF_FIG)."
TP53 bound to DACH1 increases the amount of TP53. 1 / 1
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"As DACH1 bound p53 and induced p53 target expression, we considered the possibility that DACH1 may enhance p53 dependent transcriptional activity."
TP53 bound to KMT2D increases the amount of TP53. 1 / 1
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"On one hand, KMT2D interacts directly with p53 to promote expression of p53 target genes."
Phosphorylated TP53 increases the amount of mutated TP53. 1 / 1
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"Chronic exposure to BaP induced malignant transformation of cells, in which there were increased levels of mutant p53 (mt-p53) and reduced expression of wild-type p53 (wt-p53) and phosphorylated p53 (p-p53)."