A database built with INDRA combining content from numerous readers and databases. This page allows you to curate the loaded statements. For more information please see the manual.

IndraLab

Statements

databases
phosphosite cbn pc11 biopax bel_lc signor biogrid tas lincs_drug hprd trrust | geneways tees isi trips rlimsp medscan sparser reach
reading

BRAF phosphorylates MAP2K1. 10 / 62
21 | 1 6 22 10
biopax:inoh
No evidence text available
biopax:inoh
No evidence text available
biopax:inoh
No evidence text available
reach
"We observed that 65% of anti-BRAF autoantibodies activate phosphorylation of MEK1 by BRAF in vitro."
reach
"BRAF directly phosphorylates the dual-specificity kinases MEK1 and MEK2, which in turn phosphorylate and activate the mitogen activated protein kinases, ERK1 and ERK2."
sparser
"We found that not BRAF WT but BRAF V600E dependent phosphorylation of MEK1 was increased in the presence of AA ( xref )."
reach
"BRAF proteins, Ras regulated kinase, phosphorylate MEK1 and MEK2, which in turn phosphorylate MAPK-ERK 1/2."
rlimsp
"Inhibitors may act by prevention of activation (PoA), which involves interfering with phosphorylation of nonactivated MEK1 by the upstream kinase, B-RAF."
biopax:panther
No evidence text available
sparser
"We envisioned two models for how MEK1 binding to KSR1 potentiates the ability of KSR1 to promote BRAF phosphorylation of MEK1."
BRAF phosphorylates MAP2K1 on S218. 10 / 16
1 6 1 4 | 2 1 1
biopax:pid
No evidence text available
hprd
No evidence text available
hprd
No evidence text available
sparser
"B-Raf phosphorylates MEK1 and MEK2 on Ser217 and Ser221, which activates it to dual phosphorylate ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2 [ xref , xref ]."
rlimsp
"B-Raf protein isoforms interact with and phosphorylate Mek-1 on serine residues 218 and 222. The B-raf/c-Rmil proto-oncogene belongs to the raf/mil family of serine/threonine protein kinases. It encodes multiple protein isoforms resulting from alternative splicing of two exons located upstream of the kinase domain. Recent studies suggested that B-Raf could be the intermediate molecule between Ras and Mek-1 (MAP Kinase Kinase) in signalling pathways specific of neural cells. However, there has been no evidence for a direct interaction between B-Raf and Mek-1. We report here that different B-Raf isoforms can be co-immunoprecipitated with anti-Mek-1 antisera in COS-1 cells and that the kinase activity of B-Raf is not required for its interaction with Mek-1. We also show that all B-Raf isoforms tested phosphorylate Mek-1 in a time-dependent manner, whereas kinase defective mutants fail to do so. Finally, we demonstrate that the constitutively activated S218D, S222D and S218D/S222D mutants of Mek-1 interact similarly with B-Raf. However, only the S218D and S222D mutants, and not the S218D/S222D double mutant, can be phosphorylated by B-Raf isoforms. Therefore, serine residues 218 and 222, previously shown to regulate Mek-1 activity, appear to be the major phosphorylation sites by B-Raf in vitro."
rlimsp
"B-Raf protein isoforms interact with and phosphorylate Mek-1 on serine residues 218 and 222."
biopax:pid
No evidence text available
hprd
No evidence text available
biopax:pid
No evidence text available
biopax:pid
No evidence text available
BRAF phosphorylates MAP2K1 on S222. 10 / 14
1 6 1 4 | 1 1
hprd
No evidence text available
biopax:pid
No evidence text available
signor
"Raf-1 phosphorylation of MEK activated it, as judged by its ability to stimulate the phosphorylation of myelin basic protein by glutathione S-transferase-ERK1."
sparser
"B-Raf phosphorylates MEK1 and MEK2 on Ser217 and Ser221, which activates it to dual phosphorylate ERKs, at Thr202/Tyr204 for human ERK1 and Thr185/Tyr187 for human ERK2 [ xref , xref ]."
hprd
No evidence text available
biopax:pid
No evidence text available
biopax:pid
No evidence text available
biopax:pid
No evidence text available
biopax:phosphositeplus
No evidence text available
hprd
No evidence text available
BRAF-V600E phosphorylates MAP2K1. 3 / 4
| 3
reach
"Although both BRAF V600E or BRAF WT phosphorylates MBP but such phosphorylation was not affected in the presence of increasing concentrations of acetoacetate or 3-HB (XREF_FIG and XREF_SUPPLEMENTARY), suggesting that acetoacetate may specifically affect BRAF V600E dependent phosphorylation of MEK1."
reach
"Immunoprecipitated BRAF (V600E) phosphorylated MEK1 protein in vitro (XREF_FIG, lane 5), however, treatment with 17-DMAG clearly inactivated the kinase activity of BRAF (V600E) (XREF_FIG, lane 6), even though equivalent levels of BRAF (V600E) protein were utilized from treated and non treated cell lysates (XREF_FIG, lanes 3 and 4)."
reach
"BRAF V600E increases 3-hydroxy-3-methylglutaryl-CoA lyase, intracellular levels of acetoacetate, and MEK1 phosphorylation, suggesting that there is crosstalk between metabolic pathways and cell signaling networks."
BRAF phosphorylated on T373 phosphorylates MAP2K1 on S218. 2 / 2
2 |
biopax:pid
No evidence text available
biopax:pid
No evidence text available
BRAF phosphorylated on T373 phosphorylates MAP2K1 on S222. 2 / 2
2 |
biopax:pid
No evidence text available
biopax:pid
No evidence text available
BRAF phosphorylates MAP2K1 on S252. 1 / 1
1 |
signor
"Raf-1 phosphorylation of MEK activated it, as judged by its ability to stimulate the phosphorylation of myelin basic protein by glutathione S-transferase-ERK1."
BRAF phosphorylates MAP2K1 on S248. 1 / 1
1 |
signor
"Raf-1 phosphorylation of MEK activated it, as judged by its ability to stimulate the phosphorylation of myelin basic protein by glutathione S-transferase-ERK1."