"In the present study, bioinformatics analyses and luciferase assays were employed to show that miR-466 was able to directly target the 3 '-untranslated region of insulin receptor substrate 1 (IRS1) gene, negatively regulating the mRNA and the protein expression levels of IRS1 in OS cells."
"These data suggest that miR-126 directly targets IRS-1 3 ' UTR and suppresses IRS-1 expression at the posttranscriptional level."
"Therefore, we further revealed that miR-96 targets 3 ' UTRs of INSR and IRS-1 genes directly to suppress the expression of the INSR and IRS-1 protein, resulting in impaired insulin signaling and glycogen synthesis."
"Downregulation of IRS-1 by IRS-1 siRNA decreased IRS-1 levels as expected and also the levels of phosphorylated GSK-3beta, which is downstream from IRS-1 in signal transduction."
"Our results showed that IRS1 short hairpin RNAs can effectively suppress the expression of IRS1, and inhibit the phosphorylation of AKT in IRS1 pathway; reduce the expression of MMP2, MMP3, MMP13, and MMP14, decrease the expression of TNFRSF11B and RANKL (also known as tumor necrosis factor (ligand) superfamily, member 11), however increase the RANKL and TNFRSF11B ratio; decrease cell survival, proliferation, and mineralization, and impair the differentiation of MC3T3-E1 cells."
"Transfection of IRS-1 siRNA efficiently reduced the level of IRS-1 in three lung cancer cell lines (i.e., A549, H157 and Calu-1) as detected by Western blotting (XREF_FIG A)."
"Mechanistically, insulin receptor substrate 1 (Irs1) is a direct target of miR-191, and dysregulated IRS1 expression antagonizes STAT5 activation."