IndraLab

Statements

USP15 affects TRIM25
1 | 14
USP15 deubiquitinates TRIM25. 10 / 13
1 | 12
reach
"The sfRNA of an epidemic DENV strain bound to TRIM25 in a sequence specific manner, and this interaction prevented TRIM25 deubiquitination and stabilization by USP15, thereby blunting the RIG-I-mediated IFN response [XREF_BIBR]."
27792906
reach
"Furthermore, wild-type USP15, but not its catalytically inactive C783A mutant, decreased the polyubiquitylation of TRIM25 that was caused by ectopically expressed HOIL-1L and HOIP (XREF_FIG)."
24399297
reach
"The protein levels of TRIM25 may also have to be at a certain level in order for it to productively ubiquitinate RIG-I, as the ubiquitin protease USP15 deubiquitinates TRIM25 at later time points in viral infection (109)."
31379819
ubibrowser
"Review"
27775833
reach
"Conversely, USP15 and USP4 deubiquitinate TRIM25 and RIG-I, respectively, to stabilize the proteins."
25644461
reach
"USP15 was originally identified as a DUB enzyme that interacts with TRIM25, serving to cleave the K48-ubiquitin chain that maintains TRIM25 in an inactive state; thus, de-ubiquitination of TRIM25 by USP15 potentiates TRIM25 dependent activation of RIG-I [XREF_BIBR]."
28295214
reach
"We found that upon binding to TRIM25, USP15 deubiquitylated TRIM25, which stabilized the protein and thereby led to a sustained RIG-I-dependent antiviral response (XREF_FIG)."
24399297
reach
"We next addressed whether USP15 deubiquitylated endogenous TRIM25 in the context of viral infection."
24399297
reach
"In contrast, USP15 deubiquitylates TRIM25, preventing the LUBAC dependent degradation of TRIM25 [XREF_BIBR]."
33498196
reach
"A study has demonstrated that USP15 deubiquitinates TRIM25, thus counteracting the LUBAC dependent degradation of TRIM25."
33511009
Modified USP15 leads to the deubiquitination of TRIM25. 2 / 2
| 2
reach
"In contrast, expression of wild-type USP15, but not its catalytically inactive mutant, reduced the Lys (48)-linked ubiquitylation of TRIM25, leading to its stabilization."
24399297
reach
"Furthermore, ectopic expression of USP15 substantially decreased the extent of ubiquitylation of endogenous TRIM25 in virus infected cells (XREF_FIG)."
24399297
USP15 affects BARD1
| 10
USP15 deubiquitinates BARD1. 7 / 7
| 7
reach
"Other epigenetic modifications associated with PARPi resistance includ de-ubiquitination of the BARD1 BRCT1 domain by the USP15 enzyme (52)."
34904812
reach
"Subsequently, USP15 deubiquitinates BARD1 BRCT domain, and promotes BARD1-HP1gamma interaction, resulting in BRCA1 and BARD1 retention at DSBs."
30874560
reach
"XREF_FIG, USP15 deubiquitinated the BRCT domain of BARD1 in vitro."
30874560
reach
"USP15 deubiquitinates BARD1 BRCT domain."
30874560
reach
"XREF_FIG, USP15 deubiquitinated BARD1 BRCT domain in vitro, and promoted BARD1-HP1gamma interaction."
30874560
reach
"Deubiquitination of BARD1 BRCT domain by USP15 assisted BRCA1 retention at DSBs and causes PARPi resistance [116]."
32563252
reach
"Because the C terminal of BARD1 is important for its functions in HR, we then investigated if USP15 deubiquitinates BARD1 BRCT domain in vitro and in vivo."
30874560
USP15-S678A deubiquitinates BARD1. 1 / 1
| 1
reach
"Mechanistically, USP15 S678A mutant failed to deubiquitinate BARD1 in response to MMC treatment, and in turn failed to facilitate the interaction between BARD1 and HP1gamma."
30874560
USP15-D967H deubiquitinates BARD1. 1 / 1
| 1
reach
"At the molecular level, USP15 M861V and D967H disrupted USP15-BARD1 interaction and failed to deubiquitinate BARD1 at the BRCT domain."
30874560
USP15-M861V deubiquitinates BARD1. 1 / 1
| 1
reach
"At the molecular level, USP15 M861V and D967H disrupted USP15-BARD1 interaction and failed to deubiquitinate BARD1 at the BRCT domain."
30874560
USP15 affects SMURF2
1 | 8
USP15 deubiquitinates SMURF2. 7 / 7
1 | 6
ubibrowser
"USP15 regulates SMURF2 kinetics through C-lobe mediated deubiquitination"
26435193
reach
"In light of the data presented so far we reasoned that USP15 may also directly deubiquitinate SMURF2."
26435193
reach
"We now establish that USP15 also directly deubiquitinates the E3 ligase SMURF2, opposing the activity of SMURF2 towards the TbetaR complex."
26435193
reach
"Here, we demonstrate that apart from targeting the TbetaR complex directly, USP15 also deubiquitinates SMURF2 resulting in enhanced TbetaR stability and downstream pathway activation."
26435193
reach
"Our results indicate that USP15 directly deubiquitinates SMURF2 potentially resulting in the inhibition of SMURF2 catalytic activity."
26435193
reach
"Eichhorn and co-workers found that USP15 not only targets the TGF-beta type I receptor complex but also deubiquitinates Smurf2."
27155333
reach
"To see if USP15 deubiquitinated SMURF2 in vitro, we purified USP15 and Ub-SMURF2 from extracts of HEK293T cells and proteins were mixed under conditions supporting SMURF2 deubiquitination."
26435193
USP15 deubiquitinates SMURF2 on K734. 2 / 2
| 2
reach
"USP15 deubiquitinates Smurf2 at Lys734, a residue required for Smurf2 transthiolation and catalytic activity."
33718111
reach
"These authors performed proteomic analysis and found that USP15 deubiquitinates Smurf2 on Lys734, a residue required for Smurf2 catalytic activity, leading to enhanced TGF-beta signalling (Iyengar et [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
27155333
USP15 affects NFKBIA
1 | 7
USP15 deubiquitinates NFKBIA. 7 / 7
1 | 6
reach
"CSN associated USP15 can deubiquitinate IkappaBalpha after TNFalpha mediated stimulation of the NF-kappaB pathway XREF_BIBR."
28074857
reach
"In this report, it was postulated that a CSN associated deubiquitinylase (USP15) causes a deubiquitination of IkappaBalpha representing a negative feedback mechanism of IkappaBalpha degradation and NF-kappaB activation."
19656241
reach
"Schweitzer et al. reported that COP9 signalosome (CSN)-associated USP15 can deubiquitinate IkappaBalpha and regulates the activation of NF-kappaB XREF_BIBR."
26061460
reach
"Another report reveals that USP15 deubiquitinates IkappaBalpha and increases its re-accumulation, leading to reduced NF-kappaB activity [XREF_BIBR]."
28245560
ubibrowser
No evidence text available
reach
"The Cylindroma tumour suppressor protein (CYLD) de-ubiquitinates NEMO and TRAF2 [XREF_BIBR - XREF_BIBR], while USP15 reverses betaTRCP mediated ubiquitination of IkappaBalpha [XREF_BIBR]."
22212761
reach
"USP15 deubiquitinates IkappaBalpha and promotes its re-accumulation after TNF-alpha-induced degradation, leading to reduced NF-kappaB activity."
23727018
Modified USP15 leads to the deubiquitination of NFKBIA. 1 / 1
| 1
reach
"An in vitro ubiquitination assay revealed that IkappaBalpha ubiquitination was markedly inhibited by overexpression of CHMP5 or USP15 (XREF_FIG), which is accompanied with pulse-chase labeling experiments showing that IkappaBalpha stability was increased by overexpression of CHMP5 or USP15 (XREF_FIG)."
26195726
USP15 affects DDX58
1 | 7
USP15 deubiquitinates DDX58. 5 / 5
1 | 4
reach
"USP15 specifically deubiquitinates RIG-I by direct physical association and does not interact with other signaling mediators like interferon-beta promoter stimulator 1 (IPS-1), TRAF3, and TBK1 in HEK293 cells."
33138315
reach
"Conversely, USP15 and USP4 deubiquitinate TRIM25 and RIG-I, respectively, to stabilize the proteins."
25644461
reach
"To further confirm USP15 deubiquitinates polyubiquitination of RIG-I, USP15 siRNAs were transfected into HEK293T cells to knockdown endogenous USP15 expression."
26061460
ubibrowser
"USP15 specifically removed lysine 63-linked polyubiquitin chains from RIG-I among the essential components in RIG-I-like receptor-dependent pathway."
26061460
reach
"Coimmunoprecipitation analysis showed that compared with control siRNA transfected cells, knockdown of USP15 substantially increased RIG-I polyubiquitination in WT ubiquitin and mutant Lys63 transfected cells (XREF_FIG)."
26061460
Ubiquitinated USP15 leads to the deubiquitination of DDX58. 2 / 2
| 2
reach
"As shown in Fig. 5g, USP15 knockdown greatly enhanced SEV-induced WT and K63-linked polyubiquitination of RIG-I, further confirming USP15-mediated deubiquitination of RIG-I under physiological conditions."
26061460
reach
"As shown in XREF_FIG, USP15 knockdown greatly enhanced SEV induced WT and K63 linked polyubiquitination of RIG-I, further confirming USP15 mediated deubiquitination of RIG-I under physiological conditions."
26061460
Modified USP15 leads to the deubiquitination of DDX58. 1 / 1
| 1
reach
"The overexpression of USP15-WT, but not of the deubiquitinase deficient mutants (USP15-C269A and USP15-H862A), significantly decreased the ubiquitination of RIG-I (XREF_FIG) and did not block ubiquitination of IPS-1 (XREF_FIG), TRAF3 (XREF_FIG) and TBK1 XREF_FIG), illustrating that USP15 has deubiquitination activity for RIG-I."
26061460
USP15 affects BMPR1A
1 | 7
USP15 deubiquitinates BMPR1A. 7 / 7
1 | 6
reach
"We next asked whether USP15 can deubiquitylate ALK3 in cells."
24850914
reach
"USP15 deubiquitylates ALK3."
24850914
reach
"Here, we demonstrate that USP15 interacts with and deubiquitylates the type I BMP receptor ALK3."
24850914
reach
"Here, we demonstrate that USP15 enhances BMP signalling by associating with and deubiquitylating the BMP type I receptor ALK3."
24850914
ubibrowser
"Review"
27775833
reach
"It was shown that USP15 interacts with type I BMP receptors, including ALK3, co-localises with ALK3 at the membrane and deubiquitylates ALK3, thereby countering the inhibition of the BMP pathway caused by SMAD6 XREF_BIBR."
25007997
reach
"We hypothesized that USP15 deubiquitylates ALK3, thereby opposing the effect of SMAD6 and its associated E3 ubiquitin ligases."
24850914
Mutated USP15 deubiquitinates BMPR1A. 1 / 1
| 1
reach
"To ask whether FLAG-ALK3 deubiquitylation requires the deubiquitylase activity of USP15, we tested the ability of a catalytically inactive mutant of USP15 (USP15 [C269S]; USP15-DD) to deubiquitylate FLAG-ALK3 in HEK293 cells."
24850914
USP15 affects TGFBR1
1 | 6
USP15 deubiquitinates TGFBR1. 7 / 7
1 | 6
reach
"Ubiquitin-specific peptidase 15 (USP15), a deubiquitinating enzyme, binds to the SMAD7-SMURF2 complex and deubiquitinates and stabilizes TGFBR1, resulting in enhanced TGF-β signaling ."
35017630
reach
"Preliminary results by Ten Dijke and colleagues add weight to this theory whereby they identified that the ability of USP15 to deubiquitinate TbetaRI requires USP4, as USP15 was unable to perform this[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
28923280
reach
"USP15 binds to the Smad7 and Smurf2 complex and, like USP4 and USP11, deubiquitinates the TGF-beta type I receptor, thereby maintaining the stability of this receptor and enhancing TGF-beta signalling[MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
27155333
ubibrowser
"Review"
27775833
reach
"For example, the closely related DUBs USP4, USP11 and USP15 have been reported to modulate TGFbeta signalling by deubiquitylating the type I TGFbeta receptor ALK5 [XREF_BIBR - XREF_BIBR]."
24850914
reach
"UCHL37, USP11, and USP15 de-ubiquitinate TbetaRI, XREF_BIBR, XREF_BIBR, XREF_BIBR while USP15, CYLD, and USP9X target SMAD4 or SMAD7."
27853171
reach
"It has been revealed that the DUBs, UCH37, USP11, and USP15, de-ubiquitinate and stabilize TbetaRI."
27853171
USP15 affects PRPF31
1 | 6
USP15 deubiquitinates PRPF31. 6 / 6
1 | 5
reach
"These findings suggest that USP15 deubiquitinates PRP3 as well as PRP31 when they are ubiquitinated by E3 ligase although USP15 may have more preference for PRP31."
28088760
reach
"Moreover, we found that USP15 and USP4 deubiquitinated substrates PRP31 and PRP3 simultaneously."
28088760
reach
"Moreover, it was reported that Sart3, Usp4 and Usp15 form a complex in order to de-ubiquitinate Prp3 and Prp31 simultaneously."
| PMC
reach
"Deubiquitination of PRP31 and PRP3 by the USP15, SART3, and USP4 complex decreases the affinity towards PRP8 and this regulation is important for the proper splicing of chromosome segregation related genes such as Bub1 and alpha-tubulin."
28088760
ubibrowser
"USP15 regulates dynamic protein-protein interactions of the spliceosome through deubiquitination of PRP31"
28132030
reach
"We further confirmed that the modified forms of PRP31 represented a covalent modification of PRP31 with ubiquitin using the denaturing NiNTA-pull down assay and found that USP15 WT but not inactive USP15 C269A led to deubiquitination of PRP31 in the cell."
28088760
USP15-C269A leads to the deubiquitination of PRPF31. 1 / 1
| 1
reach
"We further confirmed that the modified forms of PRP31 represented a covalent modification of PRP31 with ubiquitin using the denaturing NiNTA-pull down assay and found that USP15 WT but not inactive USP15 C269A led to deubiquitination of PRP31 in the cell."
28088760
USP15 affects PRPF3
| 7
USP15 deubiquitinates PRPF3. 7 / 7
| 7
reach
"Moreover, we found that USP15 and USP4 deubiquitinated substrates PRP31 and PRP3 simultaneously."
28088760
reach
"Moreover, it was reported that Sart3, Usp4 and Usp15 form a complex in order to de-ubiquitinate Prp3 and Prp31 simultaneously."
| PMC
reach
"Deubiquitination of PRP31 and PRP3 by the USP15, SART3, and USP4 complex decreases the affinity towards PRP8 and this regulation is important for the proper splicing of chromosome segregation related genes such as Bub1 and alpha-tubulin."
28088760
reach
"However, USP15 deubiquitinated PRP3 in the presence of E3 ligase."
28088760
reach
"PRP3 was deubiquitinated by USP15 in the presence of PRP19."
28088760
reach
"PRP3 was deubiquitinated by USP4 consistent with a previous report but not by USP15."
28088760
reach
"These findings suggest that USP15 deubiquitinates PRP3 as well as PRP31 when they are ubiquitinated by E3 ligase although USP15 may have more preference for PRP31."
28088760
USP15 affects TGFB
| 6
USP15 deubiquitinates TGFB. 5 / 5
| 5
reach
"For example, they are involved in the TGF-beta signaling pathway; USP15 deubiquitinates and stabilizes TGF-beta receptor I or receptor activated SMADs, while USP4 regulates the signaling pathway of TGF-beta receptor I and is associated with a poor prognosis in breast cancer."
28088760
reach
"For example, the closely related DUBs USP4, USP11 and USP15 have been reported to modulate TGFbeta signalling by deubiquitylating the type I TGFbeta receptor ALK5 [XREF_BIBR - XREF_BIBR]."
24850914
reach
"Moreover, Seoane and colleagues reported that the USP15 gene is amplified in breast cancer, ovarian cancer, and glioblastoma, and that USP15 deubiquitinates and stabilizes type I TGFbeta receptor, thereby enhancing TGFbeta signaling and tumor growth [XREF_BIBR] (XREF_FIG)."
27844253
reach
"USP15 binds to the SMAD7-SMAD specific E3 ubiquitin protein ligase 2 (SMURF2) complex and deubiquitinates and stabilizes type I TGF-beta receptor (TbetaR-I), leading to an enhanced TGF-beta signal."
22344298
reach
"USP15 in this trimeric complex deubiquitinates and stabilizes type 1 TGF-beta receptor, upregulating the TGF-beta signaling and providing a critically pathogenic factor for glioblastoma."
27893708
USP15 deubiquitinates TGFB on R1. 1 / 1
| 1
reach
"USP15 Enhances the Proliferation, Migration, and Collagen Deposition of Hypertrophic Scar-Derived Fibroblasts by Deubiquitinating TGF-βR1 In Vitro."
34546211
USP15 affects MDM2
1 | 4
USP15 deubiquitinates MDM2. 5 / 5
1 | 4
reach
"USP15 deubiquitylates MDM2, an E3 ligase that ubiquitylates and degrades P53 as well as NFATc2."
26732666
ubibrowser
"Review"
28801249
reach
"Considering the USP15-MDM2 direct association and the role of USP15 in stabilizing MDM2 and inhibiting MDM2 ubiquitination in activated T cells (XREF_FIG), we examined whether USP15 directly deubiquitinates MDM2."
24777531
reach
"By specifically antagonizing MDM2 auto-ubiquitination, the DUB USP15 prevents NFATc2 dependent gene activation, including IFN-gamma production."
28524749
reach
"Under normal condition, a DUB, Usp15, deubiquitinates MDM2 to inhibit MDM2 degradation, thereby negatively regulating the generation of IFN-gamma-producing Th1 effector T cells."
27012466
USP15 affects KEAP1
1 1 | 3
USP15 deubiquitinates KEAP1. 4 / 4
1 | 3
ubibrowser
"Review"
27775833
reach
"USP15 deubiquitinates Keap1."
23727018
reach
"Here we report that the deubiquitinating enzyme, USP15, specifically deubiquitinates Keap1, which suppresses the Nrf2 pathway."
23727018
reach
"USP15 activates ubiquitin ligase activity by deubiquitinating Keap1 as its adaptor, promoting the proteasomal degradation of Nrf2."
27416755
USP15 deubiquitinates KEAP1 on lysine. 1 / 1
1 |
biopax:reactome
No evidence text available
USP15 affects TGFBR
| 4
USP15 deubiquitinates TGFBR. 4 / 4
| 4
reach
"For example, they are involved in the TGF-beta signaling pathway; USP15 deubiquitinates and stabilizes TGF-beta receptor I or receptor activated SMADs, while USP4 regulates the signaling pathway of TGF-beta receptor I and is associated with a poor prognosis in breast cancer."
28088760
reach
"USP15 can deubiquitinate type I TGF-β receptor (TβR-I) and enhance TGF-β activity; and over-expression of USP15 is closely related to TGF-β activation as well as a poor prognosis for glioblastoma patients."
30319415
reach
"USP15 binds to the SMAD7-SMAD specific E3 ubiquitin protein ligase 2 (SMURF2) complex and deubiquitinates and stabilizes type I TGF-beta receptor (TbetaR-I), leading to an enhanced TGF-beta signal."
22344298
reach
"USP15 in this trimeric complex deubiquitinates and stabilizes type 1 TGF-beta receptor, upregulating the TGF-beta signaling and providing a critically pathogenic factor for glioblastoma."
27893708
USP15 affects PRKN
| 4
USP15 deubiquitinates PRKN. 4 / 4
| 4
reach
"However, we found no evidence that USP15 deubiquitinates Parkin in basal conditions or after exposure to valinomycin (Fig. 5A and B)."
24852371
reach
"At present, the negative regulatory mechanisms against Parkin-mediated ubiquitination have been reported, with the discovery of several deubiquitinases including USP8, USP15 and USP30 that are able to cause the deubiquitination of Parkin and/or OMM proteins."
29934616
reach
"USP15 does not deubiquitylate Parkin under basal conditions or when cells are treated with mitochondrial depolarizing agents."
28959952
reach
"USP15 has been shown to deubiquitinate receptor-activated SMADs and TRIM25 and antagonize Parkin-mediated mitochondrial ubiquitination, and USP15 regulates the TGF-β pathway and is a key factor in glioblastoma pathogenesis (Eichhorn et al., 2012; Pauli et al., 2014; Inui et al., 2011)."
36065190
USP15 affects NFE2L1
1 | 3
USP15 deubiquitinates NFE2L1. 4 / 4
1 | 3
reach
"Under these experimental conditions, USP15 significantly reduced the smear patterns of 3 x Flag-Nrf1, suggesting that USP15 promotes the deubiquitination of Nrf1."
27416755
ubibrowser
"Here, we report that USP15 (Ubiquitin-Specific Protease 15) activates Nrf1 in the nucleus by stabilizing it through deubiquitination."
27416755
reach
"As a result, these data strongly suggest that USP15 stabilizes endogenous NRF1.We further conducted a ubiquitination assay to examine whether USP15 deubiquitinates Nrf1."
27416755
reach
"Taken together, it is fully possible that USP15 deubiquitinates Nrf1 in the nucleus."
27416755
USP15 affects EIF4A1
| 1 3
USP15 leads to the deubiquitination of EIF4A1. 4 / 4
| 1 3
reach
"Together, these data suggested that USP15 can enhance the migration and proliferation of HaCaT cells by promoting EIF4A1 deubiquitination."
34422211
reach
"USP15 Enhances HaCaT Cell Functionality by Promoting EIF4A1 Deubiquitination."
34422211
reach
"At a mechanistic level, USP15 enhanced the functional properties of HaCaT cells by promoting EIF4A1 deubiquitination."
34422211
trips
"USP15 Enhances Re-epithelialization Through Deubiquitinating EIF4A1 During Cutaneous Wound Repair."
32671073
USP15 affects TP53
| 3
USP15 deubiquitinates TP53. 3 / 3
| 3
reach
"USP15 deubiquitinates p53."
27893708
reach
"To examine whether USP15 can deubiquitinate p53, the effect of USP15 on p53 that had been ubiquitinated by overexpressed MDM2 was determined."
27893708
reach
"The mechanism for USP15 to block p53 ubiquitination mediated by MDM2 was analyzed."
27893708
USP15 affects TOP2A
| 3
USP15 leads to the deubiquitination of TOP2A. 3 / 3
| 3
reach
"USP15 overexpression significantly reduced the endogenous ubiquitination of TOP2A, and silencing endogenous USP15 promoted TOP2A ubiquitination."
36065190
reach
"Accordingly, in the presence or absence of MG132, silencing endogenous USP15 promoted TOP2A ubiquitination (Figures 6I and 6J)."
36065190
reach
"The results revealed that USP15 significantly diminished TOP2A ubiquitination, suggesting that USP15 might be a potential TOP2A deubiquitinase (Figure 6B)."
36065190
USP15 affects SQSTM1
1 | 2
USP15 deubiquitinates SQSTM1. 3 / 3
1 | 2
ubibrowser
No evidence text available
reach
"At one point, endosomes leave the cloud, which involves the deubiquitination of SQSTM1 and p62 by the deubiquitinating enzyme USP15, and start their fast bidirectional microtubule based journey in the periphery along microtubules and finally the cortical actin cytoskeleton before reaching the cell surface."
29900632
reach
"At one point, endosomes leave the cloud, which involves the deubiquitination of SQSTM1 and p62 by the deubiquitinating enzyme USP15, and start their fast bidirectional microtubule based journey in the periphery along microtubules and finally the cortical actin cytoskeleton before reaching the cell surface."
29900632
USP15 affects SMAD3
1 1 | 1
USP15 deubiquitinates SMAD3. 1 / 1
1 |
ubibrowser
"Multiple mono-ubiquitination of SMAD3 can be removed by USP15 and mono-ubiquitination of SMAD4 seems to be removed by FAM/USP9X."
25317929
USP15 deubiquitinates SMAD3 ubiquitinated on K81 on K81. 1 / 1
| 1
reach
"In contrast, USP15 deubiquitinates mono-ubiquitinated SMAD3 at K81 and to a lesser degree at K33 and K53 suggesting that USP15 deubiquitinates R-SMADs in the MH1 domain at sites independent of SMURF2 [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
28923280
USP15 deubiquitinates SMAD3 on lysine. 1 / 1
1 |
biopax:reactome
No evidence text available
USP15 affects RORgammat
| 3
Modified USP15 leads to the deubiquitination of RORgammat. 1 / 1
| 1
reach
"Knockdown of USP15 or expression of inactive USP15 impaired Th17 differentiation, suggesting a positive role for USP15 mediated deubiquitination of RORgammat in Th17 differentiation."
27430721
USP15 deubiquitinates RORgammat. 1 / 1
| 1
reach
"To determine whether USP15 can deubiquitinate RORgammat, RORgammat was co-expressed with USP15 WT or C298A and HA-Ub in 293T cells."
27430721
USP15 deubiquitinates RORgammat on K446. 1 / 1
| 1
reach
"To determine whether USP15 can deubiquitinate RORgammat K446 specifically, K446 that has all lysines mutated to arginines except K446 was co-expressed with USP15 WT or C298A and HA-Ub in 293T cells, as we showed that K446 can be ubiquitinated (XREF_FIG)."
27430721
USP15 affects REST
1 | 2
USP15 deubiquitinates REST. 2 / 2
1 | 1
reach
"XREF_BIBR Changes in the efficiency with which USP15 deubiquitylates nascent REST, and in the cellular availability of REST during interphase, may influence expression of the broader palette of REST target genes."
23708518
ubibrowser
"Review"
28279784
Modified USP15 leads to the deubiquitination of REST. 1 / 1
| 1
reach
"Importantly, expression of GFP-USP15 could also abrogate polyubiquitylation of REST (XREF_FIG, lane 3)."
23708518
USP15 affects FHL1
| 3
USP15 deubiquitinates FHL1. 3 / 3
| 3
reach
"As we found that SLIM1 was detected in poly-ubiquitinated forms, as well as a non ubiquitinated form, when SLIM1 and Ub were co-expressed in the presence of the proteasome inhibitor MG-132 in HEK293T [MISSING/INVALID CREDENTIALS: limited to 200 char for Elsevier]"
21219870
reach
"We found a novel protein protein interaction between ubiquitin specific protease 15 (USP15) and skeletal muscle LIM protein 1 (SLIM1) : USP15 and SLIM1 directly bound under cell-free conditions and co-immunoprecipitated from the lysates of the cells, where they were co-expressed; and USP15 deubiquitinated SLIM1, resulting in the increase of protein levels of SLIM1."
21219870
reach
"In the present study, we have shown that USP15 interacts with SLIM1 and deubiquitinates SLIM1, leading to the stabilization of SLIM1."
21219870
USP15 affects BMP
| 3
USP15 deubiquitinates BMP. 3 / 3
| 3
reach
"Herhaus and coworkers showed that USP15 can interact with and deubiquitinate BMP type I receptors, thereby promoting phosphorylation of Smad1 (Herhaus et al., 2014)."
27155333
reach
"Here, we demonstrate that USP15 enhances BMP signalling by associating with and deubiquitylating the BMP type I receptor ALK3."
24850914
reach
"Here, we demonstrate that USP15 interacts with and deubiquitylates the type I BMP receptor ALK3."
24850914
USP15 affects BMI1
| 3
USP15 deubiquitinates BMI1 at position 81. 1 / 1
| 1
reach
"Further analysis showed that USP15 deubiquitinated BMI1 at lysine 81 (Figure 4F), which could also be confirmed by an in vitro deubiquitination assay (Figure 4G)."
31921558
USP15 leads to the deubiquitination of BMI1. 1 / 1
| 1
reach
"As shown in Figure 4D, USP15 knockdown in SUM159‐IL1R2 cells accelerated BMI1 degradation under CHX treatment and enhanced the ubiquitination of BMI1 protein (Figure 4E)."
31921558
USP15 deubiquitinates BMI1 on lysine. 1 / 1
| 1
reach
"Further analysis showed that USP15 deubiquitinated BMI1 at lysine 81 (Figure 4F), which could also be confirmed by an in vitro deubiquitination assay (Figure 4G)."
31921558
USP15 affects valinomycin
| 2
USP15 deubiquitinates valinomycin. 1 / 1
| 1
reach
"However, we found no evidence that USP15 deubiquitinates Parkin in basal conditions or after exposure to valinomycin (Fig. 5A and B)."
24852371
USP15 deubiquitinates valinomycin on A5. 1 / 1
| 1
reach
"However, we found no evidence that USP15 deubiquitinates Parkin in basal conditions or after exposure to valinomycin (Fig. 5A and B)."
24852371
USP15 affects TUT1
| 1 1
USP15 deubiquitinates TUT1. 2 / 2
| 1 1
trips
"USP15 deubiquitinates TUT1 associated with RNA metabolism and maintains cerebellar homeostasis."
32839293
reach
"USP15 deubiquitinates TUT1 associated with RNA metabolism and maintains cerebellar homeostasis."
32839293
USP15 affects TGFbeta receptor
| 2
USP15 deubiquitinates TGFbeta receptor. 2 / 2
| 2
reach
"Moreover, Seoane and colleagues reported that the USP15 gene is amplified in breast cancer, ovarian cancer, and glioblastoma, and that USP15 deubiquitinates and stabilizes type I TGFbeta receptor, thereby enhancing TGFbeta signaling and tumor growth [XREF_BIBR] (XREF_FIG)."
27844253
reach
"For example, the closely related DUBs USP4, USP11 and USP15 have been reported to modulate TGFbeta signalling by deubiquitylating the type I TGFbeta receptor ALK5 [XREF_BIBR - XREF_BIBR]."
24850914
USP15 affects SMAD2_3
| 2
USP15 deubiquitinates SMAD2_3. 2 / 2
| 2
reach
"USP15 has been reported to reverse SMAD2/3 monoubiquitylation, which targets the DNA binding domains of SMAD2/3 and inhibits promoter recognition."
22724073
reach
"Upon TGFbeta signaling, Usp15 deubiquitinates Smad2/3, which would lead to p97 dissociation and transcription activation."
24613342
USP15 affects SMAD2
1 1 |
USP15 deubiquitinates SMAD2. 1 / 1
1 |
ubibrowser
"reports have suggested that USP15 acts as a key regulator of TGF-b receptor-signaling pathways by deubiquitinating the TGF-b receptor itself and its downstream transducers receptor-regulated SMADs (R-SMADs), including SMAD1, SMAD2, and SMAD3, thus activating the TGF-b target genes."
28245560
USP15 deubiquitinates SMAD2 on lysine. 1 / 1
1 |
biopax:reactome
No evidence text available
USP15 affects SMAD1
1 1 |
USP15 deubiquitinates SMAD1 on lysine. 1 / 1
1 |
biopax:reactome
No evidence text available
USP15 deubiquitinates SMAD1. 1 / 1
1 |
ubibrowser
"reports have suggested that USP15 acts as a key regulator of TGF-b receptor-signaling pathways by deubiquitinating the TGF-b receptor itself and its downstream transducers receptor-regulated SMADs (R-SMADs), including SMAD1, SMAD2, and SMAD3, thus activating the TGF-b target genes."
28245560
USP15 affects RIG-I-N
| 2
USP15 leads to the deubiquitination of RIG-I-N. 1 / 1
| 1
reach
"Presence of USP15 reduced K63 linked ubiquitination of RIG-I-N in HEK293T cells (lane 3); in contrast, USP15 failed to reduce K63 linked ubiquitination of RIG-I-N in GLTSCR2 knockdown cells (lane 4)."
27824081
USP15 leads to the deubiquitination of RIG-I-N on K63. 1 / 1
| 1
reach
"Presence of USP15 reduced K63 linked ubiquitination of RIG-I-N in HEK293T cells (lane 3); in contrast, USP15 failed to reduce K63 linked ubiquitination of RIG-I-N in GLTSCR2 knockdown cells (lane 4)."
27824081
USP15 affects OMM proteins
| 2
USP15 deubiquitinates OMM proteins. 2 / 2
| 2
reach
"Parkin mediated ubiquitination of OMM proteins can be reversed by USP15 and USP30."
32213662
reach
"At present, the negative regulatory mechanisms against Parkin-mediated ubiquitination have been reported, with the discovery of several deubiquitinases including USP8, USP15 and USP30 that are able to cause the deubiquitination of Parkin and/or OMM proteins."
29934616
USP15 affects NFE2L2
| 2
USP15 leads to the deubiquitination of NFE2L2. 2 / 2
| 2
reach
"USP15 enhanced the degradation and ubiquitination of NRF2 and induced G6PD expression, leading to the progression of DNP."
35478295
reach
"USP15 promotes ubiquitination and degradation of NRF2, reducing the expression of G6PD."
35478295
USP15 affects NFATC2
| 2
USP15 leads to the deubiquitination of NFATC2. 2 / 2
| 2
reach
"A parallel HEK293 transfection experiment revealed that USP15 did not inhibit the ubiquitination of NFATc2 (XREF_SUPPLEMENTARY)."
24777531
reach
"These data suggest that USP15 promotes the ubiquitination and degradation of activated NFATc2."
24777531
USP15 affects K63-polyUb
| 2
USP15 deubiquitinates K63-polyUb. 2 / 2
| 2
reach
"Further experiment supported the idea that USP15 deubiquitinates K63-polyUb from RIG-I [XREF_BIBR]."
31208841
reach
"Further experiment supported the idea that USP15 deubiquitinates K63-polyUb from RIG-I [16]."
31208841
USP15 affects H2BC21
2 |
USP15 deubiquitinates H2BC21. 2 / 2
2 |
ubibrowser
"The U4/U6 recycling factor SART3 has histone chaperone activity and associates with USP15 to regulate H2B deubiquitination."
24526689
ubibrowser
"?Ubp-M may deubiquitinate several critical proteins that are involved in the condensation of mitotic chromosomes, mainly on ubiquitinated proteins of the chromatin such as histones H2A and H2B?"
20730552
USP15 affects DEK
| 2
USP15 leads to the deubiquitination of DEK. 2 / 2
| 2
reach
"USP15 knockdown increased DEK polyubiquitination and canceled the effect of KIF15 overexpression on reducing DEK polyubiquitination."
34280614
reach
"USP15 overexpression reduced DEK polyubiquitination."
34280614
USP15 affects CARD9
| 1 1
USP15 deubiquitinates CARD9. 2 / 2
| 1 1
reach
"USP15 Deubiquitinates CARD9 to Downregulate C-Type Lectin Receptor Mediated Signaling."
33093067
trips
"USP15 Deubiquitinates CARD9 to Downregulate C-Type Lectin Receptor-Mediated Signaling."
33093067
USP15 affects APC
1 | 1
USP15 leads to the deubiquitination of APC. 2 / 2
1 | 1
ubibrowser
"Review"
27775833
reach
"However, it has been reported that the assembly of beta-catenin destruction complex is attributed to CSN mediated deneddylation and also reliant on CSN associated USP15, which catalyzes deubiquitination of APC [XREF_BIBR]."
28245560
USP15 affects thrb
| 1
USP15 deubiquitinates thrb. 1 / 1
| 1
reach
"USP15 binds to Smurf2 complex and deubiquitinates and stabilizes TRbeta1, and that apparently leads to boosting of TGF-beta-mediated signaling."
25885904
USP15 affects procaspase-3
| 1
Modified USP15 leads to the deubiquitination of procaspase-3. 1 / 1
| 1
reach
"Overexpression of USP15 DUB in paclitaxel treated HeLa cells is shown to cause deubiquitination of procaspase-3 and its release from Cullin1 of SCF complex, resulting in apoptotic cell death."
29479529
USP15 affects p53-R273H
| 1
USP15 leads to the deubiquitination of p53-R273H. 1 / 1
| 1
reach
"In contrast, USP15 knockdown did not mediate increased ubiquitination of p53-R273H in OVCA420 cells."
29593334
USP15 affects X
| 1
USP15 deubiquitinates X. 1 / 1
| 1
reach
"To confirm the deubiquitination of HBx by USP15, we performed an in vivo ubiquitination assay in which HBx was immunoprecipitated and detected by antibody against ubiquitin."
28074857
USP15 affects VDAC1
1 |
USP15 deubiquitinates VDAC1. 1 / 1
1 |
USP15 affects USP9X
| 1
USP15 deubiquitinates USP9X. 1 / 1
| 1
reach
"UCHL37, USP11, and USP15 de-ubiquitinate TbetaRI, XREF_BIBR, XREF_BIBR, XREF_BIBR while USP15, CYLD, and USP9X target SMAD4 or SMAD7."
27853171
USP15 affects USP15
| 1
USP15 deubiquitinates USP15. 1 / 1
| 1
reach
"UCHL37, USP11, and USP15 de-ubiquitinate TbetaRI, XREF_BIBR, XREF_BIBR, XREF_BIBR while USP15, CYLD, and USP9X target SMAD4 or SMAD7."
27853171
USP15 affects TBK1
| 1
USP15 deubiquitinates TBK1. 1 / 1
| 1
reach
"Moreover, we demonstrated that SRA negatively regulates the activity of TBK1 by promoting the recruitment of ubiquitin-specific protease 15 (USP15) to deubiquitinate TBK1."
36342281
USP15 affects SRSF1
| 1
USP15 deubiquitinates SRSF1. 1 / 1
| 1
reach
"As shown in Supplementary Fig. 3A, B, SRSF1 was not deubiquitinated by USP15 or USP4, respectively."
35027535
USP15 affects SMADs
| 1
USP15 deubiquitinates SMADs. 1 / 1
| 1
reach
"For example, they are involved in the TGF-beta signaling pathway; USP15 deubiquitinates and stabilizes TGF-beta receptor I or receptor activated SMADs, while USP4 regulates the signaling pathway of TGF-beta receptor I and is associated with a poor prognosis in breast cancer."
28088760
USP15 affects SMADS
| 1
USP15 deubiquitinates SMADS. 1 / 1
| 1
reach
"Recently, It is reported that USP15 can deubiquitinate receptor activated SMADS (R-SMADS) and deubiquitination is critical for promoter recognition by SMAD complex."
23727018
USP15 affects SMAD7
| 1
USP15 deubiquitinates SMAD7. 1 / 1
| 1
reach
"UCHL37, USP11, and USP15 de-ubiquitinate TbetaRI, XREF_BIBR, XREF_BIBR, XREF_BIBR while USP15, CYLD, and USP9X target SMAD4 or SMAD7."
27853171
USP15 affects SMAD6
1 |
USP15 deubiquitinates SMAD6. 1 / 1
1 |
USP15 affects SMAD4
| 1
USP15 deubiquitinates SMAD4. 1 / 1
| 1
reach
"UCHL37, USP11, and USP15 de-ubiquitinate TbetaRI, XREF_BIBR, XREF_BIBR, XREF_BIBR while USP15, CYLD, and USP9X target SMAD4 or SMAD7."
27853171
USP15 affects SART3
1 |
USP15 deubiquitinates SART3. 1 / 1
1 |
USP15 affects RORC
1 |
USP15 deubiquitinates RORC. 1 / 1
1 |
ubibrowser
"Knockdown of USP15 or expression of inactive USP15 impaired Th17 differentiation, suggesting a positive role for USP15-mediated deubiquitination of RORγt in Th17 differentiation."
27430721
USP15 affects RNF213
| 1
USP15 deubiquitinates RNF213. 1 / 1
| 1
reach
"Moreover, the USP15 long isoform potently deubiquitylated mysterin and maintained its basal expression level, indicating that alternative exon skipping biases the deubiquitylation substrate preference of USP15."
28276505
USP15 affects RI
| 1
USP15 deubiquitinates RI. 1 / 1
| 1
reach
"Co-immunoprecipitation and ubiquitination assays revealed that USP15 interacted with TβRI and deubiquitinated TβRI."
34546211
USP15 affects RELA
| 1
Modified USP15 leads to the deubiquitination of RELA. 1 / 1
| 1
reach
"Additionally, USP15 overexpression significantly inhibited the ubiquitination of NF-kappaBp65."
30459344
USP15 affects RBX1
1 |
USP15 deubiquitinates RBX1. 1 / 1
1 |
USP15 affects R-SMAD
| 1
USP15 deubiquitinates R-SMAD. 1 / 1
| 1
reach
"Supporting our observation, one report found that USP15 deubiquitinates R-SMAD in both the cytoplasm and nucleus [14]."
27416755
USP15 affects Protease
| 1
USP15 deubiquitinates Protease on K446. 1 / 1
| 1
reach
"He et al. [130] recently reported that deubiquitination of K446 by ubiquitin-specific protease USP15 enhanced the recruitment of steroid receptor coactivator 1 (SRC1), thereby stimulating RORγt activity."
28098832
USP15 affects PPIP5K1
| 1
Modified USP15 leads to the deubiquitination of PPIP5K1. 1 / 1
| 1
reach
"The overexpression of USP15-WT, but not of the deubiquitinase deficient mutants (USP15-C269A and USP15-H862A), significantly decreased the ubiquitination of RIG-I (XREF_FIG) and did not block ubiquitination of IPS-1 (XREF_FIG), TRAF3 (XREF_FIG) and TBK1 XREF_FIG), illustrating that USP15 has deubiquitination activity for RIG-I."
26061460
USP15 affects PARP1
| 1
USP15 deubiquitinates PARP1. 1 / 1
| 1
reach
"Here, we demonstrated that the deubiquitinase USP15 interacts with and deubiquitinates PARP1 to promote its stability, thereby stimulating DNA repair, genomic stability and TNBC cell proliferation."
37012401
USP15 affects P-53
| 1
USP15 deubiquitinates P-53. 1 / 1
| 1
trips
"In the current study, we identify that USP15 binds to and stabilizes p53 through deubiquitination in U2OS and HEK293 cells."
27893708
USP15 affects NRF1
1 |
USP15 deubiquitinates NRF1. 1 / 1
1 |
ubibrowser
"we report that USP15 (Ubiquitin-Specific Protease 15) activates Nrf1 in the nucleus by stabilizing it through deubiquitination."
27416755
USP15 affects MFN2
1 |
USP15 deubiquitinates MFN2. 1 / 1
1 |
ubibrowser
No evidence text available
USP15 affects LUBAC
| 1
USP15 leads to the deubiquitination of LUBAC on K48. 1 / 1
| 1
reach
"USP15 deubiquitinates LUBAC-mediated K48-linked ubiquitination of TRIM25 and promotes the protein stability of TRIM25, there by promoting K63-linked ubiquitination of RIG-I and potentiating virus-triggered expression of type I IFN genes [82]."
25651846
USP15 affects IRS2
| 1
Modified USP15 leads to the deubiquitination of IRS2. 1 / 1
| 1
reach
"In HEK293cells, Nedd4 overexpression induced IRS-2 ubiquitination, which was decreased by USP15 co-expression while increased by USP15 knockdown."
28126338
USP15 affects H2BC10
1 |
USP15 deubiquitinates H2BC10. 1 / 1
1 |
USP15 affects H2B-Ub dimers
| 1
USP15 deubiquitinates H2B-Ub dimers. 1 / 1
| 1
reach
"Notably, human Usp15 also preferentially deubiquitinates H2A and H2B-Ub dimers and is stimulated by the splicing factor SART3, although in this case SART3 has the inverse effect : it enhances the activity of Usp15 on H2A and H2B-Ub but not on nucleosomes."
30681413
USP15 affects Dixit 2011
| 1
USP15 deubiquitinates Dixit 2011. 1 / 1
| 1
reach
"USP15 negatively regulates NFκB activation by deubiquitinating IκB (Harhaj and Dixit 2011), while USP7 decreases NFkB signalling by deubiquitinating TRAF6 and IKK (Nanduri et al. 2013)."
30244323
USP15 affects CYLD
| 1
USP15 deubiquitinates CYLD. 1 / 1
| 1
reach
"UCHL37, USP11, and USP15 de-ubiquitinate TbetaRI, XREF_BIBR, XREF_BIBR, XREF_BIBR while USP15, CYLD, and USP9X target SMAD4 or SMAD7."
27853171
USP15 affects CTNNB1
1 |
USP15 deubiquitinates CTNNB1. 1 / 1
1 |
ubibrowser
"Additionally, DUBs USP14, USP15, USP47, and Fam/USP9X have been reported to prevent β-catenin turnover by inhibiting its Ub-proteasomal degradation"
29080116
USP15 affects CRL4 CRBN
| 1
USP15 leads to the deubiquitination of CRL4 CRBN. 1 / 1
| 1
reach
"USP15 antagonizes ubiquitylation of CRL4 CRBN target proteins, thereby preventing their degradation."
34583995
USP15 affects CHUK
1 |
USP15 deubiquitinates CHUK. 1 / 1
1 |
USP15 affects CASP3
1 |
USP15 deubiquitinates CASP3. 1 / 1
1 |
USP15 affects C6orf89
| 1
USP15 deubiquitinates C6orf89. 1 / 1
| 1
reach
"Taken together, these data strongly suggest that USP15 directly deubiquitylates BRAP and thereby promotes its stability in mammalian cells."
23105109
USP15 affects BRAP
1 |
USP15 deubiquitinates BRAP. 1 / 1
1 |
USP15 affects BMP receptor
| 1
USP15 deubiquitinates BMP receptor. 1 / 1
| 1
reach
"Here, we demonstrate that USP15 interacts with and deubiquitylates the type I BMP receptor ALK3."
24850914
USP15 affects BECN1
| 1
USP15 leads to the deubiquitination of BECN1. 1 / 1
| 1
reach
"In addition, biochemical studies revealed that USP15 interacted with BECN1, but not with TRAF6, and induced deubiquitination of BECN1, thereby attenuating autophagy induction."
35422093