IndraLab

Statements

CFLAR binds USP40. 5 / 5

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"In addition, we found that overexpression of HA-GMEB1 (325–573), which did not interact with CFLAR , had no significant impact on the interaction between CFLAR and USP40 (Fig. 5c)."

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"GMEB1 promoted the binding of USP40 with CFLAR , conferring a role as an adaptor protein."

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"Data from co-IP assay in 293FT cells show that knock down of GMEB1 weakened the interaction between CFLAR and USP40; overexpression of GMEB1 enhanced the interaction (Fig. 5a and b, respectively)."

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"We found the de-ubiquitination enzyme USP40 bound to CFLAR and GMEB, which enhanced the interaction between USP40 and CFLAR resulting in reduced ubiquitination and degradation of CFLAR ."

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"GMEB1 promoted the binding of USP40 to CFLAR L ."

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CFLAR binds USP40. 3 / 3

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"GMEB1 promoted the interaction between USP40 and CFLAR L ."

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"In addition, we found that overexpression of HA-GMEB1 (325–573), which did not interact with CFLAR L , had no significant impact on the interaction between CFLAR L and USP40 (Fig. xref c)."

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"Data from co-IP assay in 293FT cells show that knock down of GMEB1 weakened the interaction between CFLAR L and USP40; overexpression of GMEB1 enhanced the interaction (Fig. xref a and b, respectively)."

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USP40 activates CFLAR.

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USP40 activates CFLAR. 5 / 5

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"Consequently, USP40 augmented the stability of CFLAR via its de-ubiquitinase activity."

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"These findings demonstrate USP40 promotes the stability of CFLAR at the protein level."

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"Using a reverse experiment in A549 cell lines, we showed that USP40 overexpression slightly increased CFLAR protein when GMEB1 was knocked down (Fig. 5e)."

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"USP40 targeted CFLAR L for K48 linked de-ubiquitination."

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"We found that GMEB1 promoted the stability of CFLAR by de-ubiquitinase USP40."

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USP40 increases the amount of CFLAR.

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USP40 increases the amount of CFLAR. 2 / 2

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"Conversely, overexpression of USP40 in H1299 and A549 cell lines increased the protein level of CFLAR (Fig. 4c)."

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"And, overexpression of USP40 increased the protein level of CFLAR though GMEB1 was knocked down."

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USP40-C62A increases the amount of CFLAR. 1 / 1

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"Data show mutant USP40 (C62A) did not increase the protein level of CFLAR in A549 cell lines (Additional file 1: Figure S4B), but it still interacted with CFLAR (Additional file 1: Figure S4C)."

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USP40 ubiquitinates CFLAR.

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USP40 leads to the ubiquitination of CFLAR. 2 / 2

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"Results show that overexpression of USP40 decreased the ubiquitination of CFLAR (Fig. 4d), and knockdown USP40 increased the ubiquitination of CFLAR (Fig. 4e)."

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"We proposed that USP40 modulated the ubiquitination of CFLAR via its de-ubiquitinase activity."

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USP40 deubiquitinates CFLAR.

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USP40-C62A deubiquitinates CFLAR. 1 / 1

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"GST pull-down assay results show mutant USP40 (C62A) did not de-ubiquitinate CFLAR (Additional file 1: Figure S4D)."

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USP40 leads to the deubiquitination of CFLAR. 1 / 1

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"Results show that overexpression of USP40 decreased the ubiquitination of CFLAR (Fig. 4d), and knockdown USP40 increased the ubiquitination of CFLAR (Fig. 4e)."

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USP40 decreases the amount of CFLAR.

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USP40 decreases the amount of CFLAR. 2 / 2

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"USP40 knockdown did not increase the protein level of CFLAR even though GMEB1 protein level was overexpressed."

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"USP40 knockdown did not increase CFLAR L protein level despite GMEB1 overexpression, suggesting GMEB1 promotes CFLAR L stability via USP40."

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USP40 affects HINT1

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USP40 deubiquitinates HINT1. 2 / 2

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"In conclusion, USP40 bound to Nestin deubiquitinates HINT1, and in consequence upregulates p53."

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"USP40 deubiquitinates HINT1 and stabilizes p53 in podocyte damage."

35605301

USP40 deubiquitinates HINT1 on histidine. 1 / 1

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"We also found that USP40 deubiquitinated histidine triad nucleotide-binding protein 1 (HINT1), an inducer of p53."

35605301

USP40 affects GMEB1

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USP40 binds GMEB1.

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USP40 binds GMEB1. 2 / 2

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"We then examined the interaction between GMEB1 and USP40 using Co-IP assay and found that tagged GMEB1 directly interacted with tagged USP40, which was confirmed by a reverse experiment (Additional file 1: Figure S3A and B)."

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"Glucocorticoid modulatory element binding protein 1 (GMEB1) interacts with the de-ubiquitinase USP40 to stabilize CFLAR"

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USP40 activates GMEB1.

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USP40 activates GMEB1. 1 / 1

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"We found that GMEB1 promoted the stability of CFLAR by de-ubiquitinase USP40."

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Pirinixic acid affects USP40

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Pirinixic acid increases the amount of USP40. 2 / 2

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ctd

No evidence text available

21318169

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ctd

No evidence text available

18301758

Bisphenol A affects USP40

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Bisphenol A increases the amount of USP40.

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Bisphenol A increases the amount of USP40. 1 / 1

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ctd

No evidence text available

29275510

Bisphenol A decreases the amount of USP40.

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Bisphenol A decreases the amount of USP40. 1 / 1

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ctd

No evidence text available

30816183

USP40 affects TP53

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USP40 deubiquitinates TP53.

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USP40 deubiquitinates TP53 on histidine. 1 / 1

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"We also found that USP40 deubiquitinated histidine triad nucleotide-binding protein 1 (HINT1), an inducer of p53."

35605301

USP40 activates TP53.

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USP40 bound to NES activates TP53. 1 / 1

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"In conclusion, USP40 bound to Nestin deubiquitinates HINT1, and in consequence upregulates p53."

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USP40 affects NES

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USP40 binds NES. 2 / 2

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"In conclusion, USP40 bound to Nestin deubiquitinates HINT1, and in consequence upregulates p53."

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"In glomerular endothelial cells, immunoprecipitation confirmed actual protein protein binding of USP40 with nestin, and USP40-small-interfering RNA transfection revealed significant reduction of nestin."

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GMEB1 affects USP40

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USP40 binds GMEB1. 2 / 2

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"Glucocorticoid modulatory element binding protein 1 (GMEB1) interacts with the de-ubiquitinase USP40 to stabilize CFLAR"

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Vinclozolin affects USP40

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Vinclozolin increases the amount of USP40. 1 / 1

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ctd

No evidence text available

23034163

Valproic acid affects USP40

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Valproic acid increases the amount of USP40. 1 / 1

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ctd

No evidence text available

17183730

Urethane affects USP40

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Urethane decreases the amount of USP40. 1 / 1

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ctd

No evidence text available

28818685

Tetrachloromethane affects USP40

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Tetrachloromethane decreases the amount of USP40. 1 / 1

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ctd

No evidence text available

31150632

Sunitinib affects USP40

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Sunitinib increases the amount of USP40. 1 / 1

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ctd

No evidence text available

31533062

Schizandrin B affects USP40

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Schizandrin B decreases the amount of USP40. 1 / 1

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ctd

No evidence text available

31150632

Phorbol 13-acetate 12-myristate affects USP40

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Phorbol 13-acetate 12-myristate decreases the amount of USP40. 1 / 1

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ctd

No evidence text available

25613284

Pentabromodiphenyl ether affects USP40

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Pentabromodiphenyl ether decreases the amount of USP40. 1 / 1

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ctd

No evidence text available

23948077

Paracetamol affects USP40

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Paracetamol decreases the amount of USP40. 1 / 1

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ctd

No evidence text available

21420995

Leflunomide affects USP40

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Leflunomide decreases the amount of USP40. 1 / 1

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ctd

No evidence text available

28988120

Lead diacetate affects USP40

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Lead diacetate increases the amount of USP40. 1 / 1

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ctd

No evidence text available

20542052

Lead atom affects USP40

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Lead atom decreases the amount of USP40. 1 / 1

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ctd

No evidence text available

19921347

Ionomycin affects USP40

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Ionomycin decreases the amount of USP40. 1 / 1

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ctd

No evidence text available

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Indometacin affects USP40

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Indometacin decreases the amount of USP40. 1 / 1

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ctd

No evidence text available

28628672

Hydralazine affects USP40

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Hydralazine increases the amount of USP40. 1 / 1

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ctd

No evidence text available

17183730

Hsa-miR-484 affects USP40

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Hsa-miR-484 decreases the amount of USP40. 1 / 1

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biopax:mirtarbase

No evidence text available

23622248

Hexabromocyclododecane affects USP40

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Hexabromocyclododecane increases the amount of USP40. 1 / 1

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ctd

No evidence text available

29177809

Glycidamide affects USP40

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Glycidamide increases the amount of USP40. 1 / 1

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ctd

No evidence text available

17822822

Dioxygen affects USP40

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Dioxygen increases the amount of USP40. 1 / 1

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ctd

No evidence text available

22629407

Dibutyl phthalate affects USP40

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Dibutyl phthalate decreases the amount of USP40. 1 / 1

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ctd

No evidence text available

21266533

Dexamethasone affects USP40

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Dexamethasone decreases the amount of USP40. 1 / 1

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ctd

No evidence text available

28628672

Cyclosporin A affects USP40

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Cyclosporin A decreases the amount of USP40. 1 / 1

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ctd

No evidence text available

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Coumestrol affects USP40

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Coumestrol decreases the amount of USP40. 1 / 1

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ctd

No evidence text available

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Calcitriol affects USP40

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Calcitriol increases the amount of USP40. 1 / 1

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No evidence text available

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Butanal affects USP40

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Butanal decreases the amount of USP40. 1 / 1

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ctd

No evidence text available

26079696

Bisphenol F affects USP40

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Bisphenol F decreases the amount of USP40. 1 / 1

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ctd

No evidence text available

28628672

Aflatoxin B1 affects USP40

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Aflatoxin B1 increases the amount of USP40. 1 / 1

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ctd

No evidence text available

19770486

USP40 affects UBC

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UBC binds USP40. 1 / 1

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biogrid

No evidence text available

28190767

USP40 affects Parkinson Disease

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USP40 inhibits Parkinson Disease. 1 / 1

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"Interestingly, it has been reported that some polymorphisms of USP24 and USP40 significantly decrease the risk of developing PD [22,23]."

26797011

USP40 affects IL10

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USP40 activates IL10. 1 / 1

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"Knockdown of USP40 significantly decreased ManLAM and Pam3CSK4 induced IL-10 production by WT B cells, whereas IL-10 production by USP40 silenced TLR2 -/- B cells was not reduced compared with scramble RNA treated TLR2 -/- B cells upon stimulation with ManLAM (XREF_FIG C, 5D, and XREF_SUPPLEMENTARY F)."

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USP40 affects GMEB

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USP40 binds GMEB. 1 / 1

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"We found the de-ubiquitination enzyme USP40 bound to CFLAR and GMEB, which enhanced the interaction between USP40 and CFLAR resulting in reduced ubiquitination and degradation of CFLAR ."

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USP40 affects DDB2

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USP40 binds DDB2. 1 / 1

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hprd

No evidence text available

11673459

USP40 affects DDB1

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USP40 binds DDB1. 1 / 1

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hprd

No evidence text available

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UBC affects USP40

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UBC binds USP40. 1 / 1

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biogrid

No evidence text available

28190767

TLR2 affects USP40

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TLR2 bound to ManLAM activates USP40. 1 / 1

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"Molecular mechanism analysis reveals that the interaction of ManLAM and TLR2 induces IL-10 production by B cells mainly via the TLR2/MyD88/PI3K/AKT/AP -1 and NEMO K63 ubiquitination NF-kappaB signaling pathways, and increased USP40."

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STAT6 affects USP40

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STAT6 decreases the amount of USP40. 1 / 1

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biopax:msigdb

No evidence text available

STAT5A affects USP40

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STAT5A decreases the amount of USP40. 1 / 1

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biopax:msigdb

No evidence text available

Phthalic Acids affects USP40

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Phthalic Acids decreases the amount of USP40. 1 / 1

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ctd

No evidence text available

21061450

ManLAM-TLR2 affects USP40

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ManLAM-TLR2 activates USP40. 1 / 1

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"ManLAM-TLR2 induces USP40 increase, which positively regulated the IL-10 production in B cells (XREF_FIG B-5D and XREF_SUPPLEMENTARY C-S5F)."

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GSK-J4 affects USP40

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GSK-J4 decreases the amount of USP40. 1 / 1

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ctd

No evidence text available

29301935

GMEB affects USP40

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USP40 binds GMEB. 1 / 1

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"We found the de-ubiquitination enzyme USP40 bound to CFLAR and GMEB, which enhanced the interaction between USP40 and CFLAR resulting in reduced ubiquitination and degradation of CFLAR ."

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